作者: nostrum 時(shí)間: 2025-3-21 22:40
Mouldy Sioud,Lina Cekaitef these genes (Wong and Neumann, 1982; Neumann ., 1982; Potter ., 1984) has opened up new possibilities in molecular biology and genetic engineering research. Electric modification of cell membrane permeability, or “electroporation,” is believed to be the basis of the DNA entry. No less an accomplis作者: Matrimony 時(shí)間: 2025-3-22 04:08
Seungil Ro,Wei Yantic phenomena attributed to electroporation include rupture, reversible electrical breakdown, the transient high-permeability state, and electrical fusion. All are associated with transmembrane potentials significantly larger than cellular resting potentials. Generally, a theory of electroporation s作者: carbohydrate 時(shí)間: 2025-3-22 05:29
Rui Song,Seungil Ro,Wei Yan recent years, dielectrophoresis has developed rapidly and its present-day field of application is broad and diverse both in inanimate and in biological systems. For example, using dielectrophoresis it may be possible to filter nonconductive liquids (Fielding ., 1975), to separate mineral powder mix作者: 濕潤(rùn) 時(shí)間: 2025-3-22 11:50 作者: 冒號(hào) 時(shí)間: 2025-3-22 13:46
Jet-Injection of Short Hairpin RNA-Encoding Vectors into Tumor Cells,e corresponding MDR1/P-gp protein is no longer detectable in the tumors after anti-MDR1 shRNA vector injection. Furthermore, combination of two intratumoral jet-injections of anti-MDR1 shRNA vectors with two intravenous administrations of doxorubicin is sufficient for a complete reversal of the MDR 作者: GOAT 時(shí)間: 2025-3-22 17:06 作者: entice 時(shí)間: 2025-3-23 00:43 作者: Tracheotomy 時(shí)間: 2025-3-23 04:10 作者: limber 時(shí)間: 2025-3-23 08:48 作者: Bucket 時(shí)間: 2025-3-23 10:33 作者: 小丑 時(shí)間: 2025-3-23 17:30 作者: 過(guò)剩 時(shí)間: 2025-3-23 20:51 作者: 分發(fā) 時(shí)間: 2025-3-23 23:59
Seungil Ro,Wei Yandding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.作者: Insul島 時(shí)間: 2025-3-24 03:17 作者: 戰(zhàn)勝 時(shí)間: 2025-3-24 08:33
Debra J. Taxman,Chris B. Moore,Elizabeth H. Guthrie,Max Tze-Han Huang作者: GIST 時(shí)間: 2025-3-24 11:11 作者: seruting 時(shí)間: 2025-3-24 15:14
Christina S?ten Fjeldbo,Kristine Misund,Clara-Cecilie Günther,Mette Langaas,Tonje Str?mmen Steigedal作者: 緊張過(guò)度 時(shí)間: 2025-3-24 20:02 作者: SLING 時(shí)間: 2025-3-24 23:23
1064-3745 ymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful .Methods in Molecular Biology.? series format, the cutting-edge protocol chapters contain introductions to their re978-1-4939-6151-1978-1-60761-657-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 繁榮地區(qū) 時(shí)間: 2025-3-25 04:30 作者: neolith 時(shí)間: 2025-3-25 09:35 作者: 咒語(yǔ) 時(shí)間: 2025-3-25 14:18
https://doi.org/10.1007/978-1-60761-657-3DNA; Microarray; PCR; Transplantat; Tumor; cloning; gene expression; genes; hybridization; miRNAs; molecular b作者: Heretical 時(shí)間: 2025-3-25 19:06
Progress in siRNA Delivery Using Multifunctional Nanoparticles,icles need to efficiently encapsulate siRNA, actively target sites of interest, and release siRNA intracellularly. This chapter reviews recent progress using a multifunctional approach to design and engineer polymeric nanoparticles for siRNA delivery.作者: 捐助 時(shí)間: 2025-3-26 00:00
Functional Studies on RNA-Transfected Cell Microarrays,ays of transfected cells are formed in which the phenotypic consequences of gene suppression can be investigated. Using reporter plasmids with fluorescence intensity as output data, we have developed a strategy for statistical analysis of the intensity data from medium-scale functional studies using data from several experimental replicates.作者: 模仿 時(shí)間: 2025-3-26 01:14 作者: CRACK 時(shí)間: 2025-3-26 08:00 作者: Mortal 時(shí)間: 2025-3-26 09:26 作者: Blood-Vessels 時(shí)間: 2025-3-26 14:37
Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1, three separate siRNA sequences targeting unique sites in HIV-1. This methodological overview explains some crucial aspects of lhRNA design and cloning as well as facile experiments to determine their efficacy in cell culture.作者: 放棄 時(shí)間: 2025-3-26 17:29
Profiling of miRNA Expression and Prediction of Target Genes,. This chapter highlights the technical advances in miRNA gene profiling and discusses the limitations of target prediction programs and the necessity of defining the roles that govern miRNA specificity and target discrimination in vivo.作者: 值得 時(shí)間: 2025-3-26 22:05
In Situ Hybridization Detection of microRNAs,scribe a protocol for miRNA in situ hybridization on mouse tissue cryosections using locked nucleic acid (LNA) probes. LNA probes demonstrate a much higher affinity to their complimentary RNAs compared to conventional RNA and DNA oligo probes, which allow detection of miRNAs in tissue sections with excellent specificity.作者: 睨視 時(shí)間: 2025-3-27 01:19
1064-3745 rapeutic proteins.Features the translation of RNA-based ther.Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutic作者: magnanimity 時(shí)間: 2025-3-27 06:54
Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interfereduced the number of unwanted off-target genes without interfering with siRNA silencing potency of the anticipated target gene. This chapter describes how to separate gene silencing from immunostimulation. Also, it discusses the impact of these findings on the design of effective cancer vaccines.作者: Gastric 時(shí)間: 2025-3-27 10:21 作者: 分開(kāi) 時(shí)間: 2025-3-27 14:47
Quantitative Analysis of RNA Modifications,. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of techniques, including oligonucleotide-affinity selection, site-specific cleavage and radiolabeling, nuclease digestion, and thin layer chromatography.作者: 背信 時(shí)間: 2025-3-27 20:46
Systemic Delivery of Synthetic siRNAs,llenge have been described, including cationic lipids, high-pressure injection, viral vectors, and chemical modifications of the siRNAs. This chapter describes cationic lipid delivery of siRNAs to adult mice.作者: 半球 時(shí)間: 2025-3-28 00:46
Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migratio cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.作者: 侵略 時(shí)間: 2025-3-28 05:45 作者: 講個(gè)故事逗他 時(shí)間: 2025-3-28 09:58 作者: 軟膏 時(shí)間: 2025-3-28 11:18
RNA Modifications: A Mechanism that Modulates Gene Expression,l modifications, including 2?-O-methylation and pseudouridylation, which are functionally important. In this chapter, we highlight the functional aspects of the modifications of spliceosomal snRNA and rRNA and provide a framework for understanding how posttranscriptional modifications are capable of influencing gene expression.作者: lymphedema 時(shí)間: 2025-3-28 16:25
Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells,ation, function, and survival can be modulated by small interfering RNAs (siRNAs) targeting genes involved in immune suppression. This chapter describes detailed protocols for introducing siRNAs into human monocytes and dendritic cells using standard electroporation techniques.作者: 拒絕 時(shí)間: 2025-3-28 21:24
Inhibition of the microRNA Pathway in Zebrafish by siRNA,this important vertebrate model because of unspecific developmental defects. We have recently shown that these side effects can be attributed to inhibition of the miRNA pathway by siRNAs at early embryonic stages. This review highlights these findings and the function of microRNAs in zebrafish development.作者: admission 時(shí)間: 2025-3-29 02:16
RNA Modifications: A Mechanism that Modulates Gene Expression,and protein translation are two major steps in eukaryotic gene expression that necessitate the production of accurate gene products. Both processes occur in large complexes, consisting of both proteins and noncoding RNAs. Interestingly, the RNA components contain a large number of posttranscriptiona作者: expository 時(shí)間: 2025-3-29 06:31
Quantitative Analysis of RNA Modifications, modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of te作者: 縱火 時(shí)間: 2025-3-29 07:27 作者: 誹謗 時(shí)間: 2025-3-29 14:12
Progress in siRNA Delivery Using Multifunctional Nanoparticles,icles need to efficiently encapsulate siRNA, actively target sites of interest, and release siRNA intracellularly. This chapter reviews recent progress using a multifunctional approach to design and engineer polymeric nanoparticles for siRNA delivery.作者: 膝蓋 時(shí)間: 2025-3-29 17:37
Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells, Unfortunately, its curative potential is in general limited by immunosuppressive proteins that negatively regulate dendritic (DC) and/or T-cell function. The recent discovery of RNA interference (RNAi) has facilitated the study of gene function in immune cells and recent data indicate that DC matur作者: 放肆的你 時(shí)間: 2025-3-29 21:43 作者: 逢迎春日 時(shí)間: 2025-3-30 02:12 作者: 變白 時(shí)間: 2025-3-30 07:21
Using OligoWalk to Identify Efficient siRNA Sequences,ression of specific mRNAs. In practice, a number of factors influence whether an siRNA sequence will elicit RNAi and knockdown target gene expression. One factor that significantly influences the efficiency of an siRNA is the effect of RNA secondary structure. Self-structure in either the siRNA sequ作者: GROUP 時(shí)間: 2025-3-30 11:38 作者: Ferritin 時(shí)間: 2025-3-30 14:15 作者: 儲(chǔ)備 時(shí)間: 2025-3-30 18:26
Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1, mutants, however, is a major impediment to the use of RNAi effectors to treat highly mutable viruses such as HIV-1. A combinatorial approach is therefore required for long-term inhibition of gene expression. RNA Pol III-driven long hairpin RNA (lhRNA) duplexes can be cleaved several times by Dicer,作者: 上腭 時(shí)間: 2025-3-30 22:05 作者: Vulnerable 時(shí)間: 2025-3-31 02:37 作者: 碎石 時(shí)間: 2025-3-31 08:46 作者: Nonconformist 時(shí)間: 2025-3-31 09:56 作者: 防水 時(shí)間: 2025-3-31 13:55
Profiling of miRNA Expression and Prediction of Target Genes, of human genes, thus underlying their influence on almost all cellular pathways. Recent studies have shown miRNAs to be differentially expressed in cancers. Furthermore, several miRNAs are associated with fragile sites, preferential sites of translation, deletion and amplification that are often al作者: 要控制 時(shí)間: 2025-3-31 21:18 作者: 神圣將軍 時(shí)間: 2025-3-31 22:54 作者: 爆米花 時(shí)間: 2025-4-1 02:58
Detection and Quantitative Analysis of Small RNAs by PCR, critical biological processes. Functional studies of these small RNAs require a simple, sensitive, and reliable method for detecting and quantifying levels of small RNAs. Here, we describe such a method that has been widely used for the validation of cloned small RNAs and also for quantitative anal作者: 煩人 時(shí)間: 2025-4-1 08:29 作者: 屈尊 時(shí)間: 2025-4-1 12:01 作者: GILD 時(shí)間: 2025-4-1 14:34
Mises and the Problem of Induction, in the social sciences. This involves a rejection of behaviorism. (3) Finally, all holistic, essentialist, and even many macroeconomic approaches contradict Mises’ individualism and are thus rejected. Further traits of Mises’ philosophy of science include realism, Wertfreiheit (value freedom), and a rejection of polylogism.作者: 整潔漂亮 時(shí)間: 2025-4-1 18:45
Book 1998rkl?ren k?nnen. Diese Modelle zeichnen sich dadurch aus, da? sie wichtige stilisierte Fakten wie die Mean-reversion des Sozialprodukts als Folge eines transitorischen Technologieschocks reproduzieren k?nnen, wozu vergleichbare Modelle nicht in der Lage sind.
