標(biāo)題: Titlebook: RNA Structure Determination; Methods and Protocol Douglas H. Turner,David H. Mathews Book 2016 Springer Science+Business Media New York 201 [打印本頁] 作者: abandon 時間: 2025-3-21 18:48
書目名稱RNA Structure Determination影響因子(影響力)
作者: 帶來墨水 時間: 2025-3-21 21:09
Secondary Structure Prediction of Single Sequences Using RNAstructure, energy minimization, prediction of base pairing probabilities, prediction of structures composed of highly probably base pairs, and prediction of structures with pseudoknots. A user-friendly graphical user interface is provided, and this interface works on Windows, Apple OS X, and Linux. This chapt作者: DAMP 時間: 2025-3-22 01:11
Prediction of Secondary Structures Conserved in Multiple RNA Sequences, much more accurate than predicting the structure for a single sequence. RNAstructure is a software package that includes the programs Dynalign, Multilign, TurboFold, and PARTS for predicting conserved RNA secondary structure. This chapter provides protocols for using these programs.作者: 感激小女 時間: 2025-3-22 04:52
,Predicting RNA–RNA Interactions Using RNAstructure,ause of the competition between intramolecular and intermolecular structure formation. A complete picture of RNA–RNA binding includes an understanding of single-stranded folding and binding site accessibility, and is strongly concentration-dependent. This chapter provides guidance for using RNAstruc作者: 含糊其辭 時間: 2025-3-22 12:45 作者: 銀版照相 時間: 2025-3-22 16:13 作者: 我不明白 時間: 2025-3-22 18:28
Traditional Chemical Mapping of RNA Structure In Vitro and In Vivo,gands. The method monitors the reactivity of each nucleotide towards chemicals of various specificities reflecting the hydrogen bonding environment of each nucleotide within the RNA molecule. In addition, information can be obtained on the binding site of a ligand (noncoding RNAs, protein, metabolit作者: 語言學(xué) 時間: 2025-3-23 00:28
High-Throughput Nuclease Probing of RNA Structures Using FragSeq,ultaneously. Such assays accelerate RNA structure analysis and provide researchers with new technologies to tackle biological questions on a transcriptome-wide scale. FragSeq is an experimental assay for transcriptome-wide RNA structure probing using RNA-Seq, coupled with data analysis tools that al作者: 中和 時間: 2025-3-23 02:21 作者: 使高興 時間: 2025-3-23 06:22
Experiment-Assisted Secondary Structure Prediction with RNAstructure,age of enzymatic cleavage data, FMN cleavage data, traditional chemical modification reactivity data, and SHAPE reactivity data for secondary structure modeling. This chapter provides protocols for using experimental probing data with RNAstructure to restrain or constrain RNA secondary structure pre作者: 招待 時間: 2025-3-23 11:51
RNA Secondary Structure Determination by NMR,lding space. Chemical mapping results typically restrain certain nucleotides not to be in AU or GC pairs. Two-dimensional nuclear magnetic resonance (NMR) spectra can reveal the order of AU, GC, and GU pairs in double helixes. This chapter describes a program, NMR-assisted prediction of secondary st作者: cornucopia 時間: 2025-3-23 17:04
Modeling Small Noncanonical RNA Motifs with the Rosetta FARFAR Server,f only ten nucleotides in size. Unfortunately, despite their small size, there is no reliable method to determine the ensemble of lowest energy structures of junctions and loops at atomic accuracy. This chapter outlines straightforward protocols using a webserver for Rosetta Fragment Assembly of RNA作者: lanugo 時間: 2025-3-23 20:13
Automated RNA 3D Structure Prediction with RNAComposer,experimentally assessing structures of large RNAs using NMR, X-ray crystallography, or cryo-microscopy, there is currently great demand for new high-resolution 3D structure prediction methods. Recently we reported on RNAComposer, a knowledge-based method for the fully automated RNA 3D structure pred作者: 加花粗鄙人 時間: 2025-3-24 00:17 作者: 鐵砧 時間: 2025-3-24 06:02 作者: ostracize 時間: 2025-3-24 06:46 作者: Dissonance 時間: 2025-3-24 13:25
The Quick and the Dead: A Guide to Fast Phasing of Small Ribozyme and Riboswitch Crystal Structures folds. Visualization of such molecules at near-atomic resolution can enhance our understanding of how chemical groups are organized spatially, thereby providing novel insight into function. This approach has its challenges, which mainly entail sample crystallization followed by the application of e作者: 用不完 時間: 2025-3-24 15:37
Modeling Small Noncanonical RNA Motifs with the Rosetta FARFAR Server, with Full Atom Refinement (FARFAR) (.) to model the 3D structure of small noncanonical RNA motifs for use in visualizing motifs and for further refinement or filtering with experimental data such as NMR chemical shifts.作者: 絕緣 時間: 2025-3-24 22:20 作者: LITHE 時間: 2025-3-25 00:20 作者: 弄污 時間: 2025-3-25 05:45 作者: 狂熱文化 時間: 2025-3-25 08:54 作者: integrated 時間: 2025-3-25 14:31
Crumple: An Efficient Tool to Explore Thoroughly the RNA Folding Landscape,method is independent of free-energy minimization and allows the user to incorporate experimental data such as chemical probing, SELEX data on RNA–protein binding motifs, and phylogenetic covariation.作者: 亞麻制品 時間: 2025-3-25 16:43 作者: Hyperlipidemia 時間: 2025-3-25 23:06
Traditional Chemical Mapping of RNA Structure In Vitro and In Vivo,an be obtained either by using end-labeled RNA molecules or by primer extension using reverse transcriptase. The goal of this chapter is to provide the reader with an experimental guide to probe the structure of RNA in vitro and in vivo with the most suitable chemical probes.作者: Albumin 時間: 2025-3-26 03:30 作者: 慎重 時間: 2025-3-26 05:47 作者: 盲信者 時間: 2025-3-26 10:17
Book 2016ary structure prediction, single sequence modeling, Crumple,? RNAstructure to model conserved secondary structures with multiple homologs,? the prediction of bimolecular secondary structures with RNAstructure, STarMir, protocols for structure mapping, mapping data to constrain or restrain RNA second作者: constitutional 時間: 2025-3-26 14:07 作者: 專橫 時間: 2025-3-26 18:34
Zhenjiang Zech Xu,David H. Mathewsn including cost optimisation;..- quality assurance and statistical process analysis and control;..- reliability models for electronic products and..- assembly accuracy..978-1-84996-586-6978-1-84628-354-3作者: 易彎曲 時間: 2025-3-26 23:05
Zhenjiang Zech Xu,David H. Mathewsn including cost optimisation;..- quality assurance and statistical process analysis and control;..- reliability models for electronic products and..- assembly accuracy..978-1-84996-586-6978-1-84628-354-3作者: 飛來飛去真休 時間: 2025-3-27 04:40 作者: 細(xì)節(jié) 時間: 2025-3-27 05:55 作者: Uncultured 時間: 2025-3-27 11:00 作者: miscreant 時間: 2025-3-27 16:02 作者: 博識 時間: 2025-3-27 18:05 作者: BIPED 時間: 2025-3-27 22:06 作者: 食草 時間: 2025-3-28 04:13 作者: Figate 時間: 2025-3-28 09:28
Jonathan L. Chen,Stanislav Bellaousov,Douglas H. Turnern including cost optimisation;..- quality assurance and statistical process analysis and control;..- reliability models for electronic products and..- assembly accuracy..978-1-84996-586-6978-1-84628-354-3作者: 隨意 時間: 2025-3-28 11:57 作者: 混雜人 時間: 2025-3-28 16:10 作者: Excise 時間: 2025-3-28 22:24 作者: 合同 時間: 2025-3-28 23:45 作者: Exclaim 時間: 2025-3-29 03:20
Pawel Piatkowski,Joanna M. Kasprzak,Deepak Kumar,Marcin Magnus,Grzegorz Chojnowski,Janusz M. Bujnickion. The voltage applied to the transformer is, of course, alternating since otherwise the magnetic field is not continuously changing. A transformer is an extremely useful circuit component because of the job it does and because it is extremely efficient and easy to maintain. Transformers are widel作者: LITHE 時間: 2025-3-29 08:23
Paul Dallaire,Fran?ois Majorommon emitter, common base and common collector (or emitter follower); and for the FET there is the common source, common gate and common drain (or source follower). It is found on comparison.between similar modes (for example, common emitter, common source) that the overall amplifier characteristic作者: 殘酷的地方 時間: 2025-3-29 11:34 作者: CANE 時間: 2025-3-29 16:54
Jermaine L. Jenkins,Joseph E. Wedekindine the chemical energy of petrol is converted to the mechanical energy of movement of the car, in the electrical generator mechanical energy of whatever drives the generator is turned into electrical energy (see chapter 4) and in the electric fire or the electric lamp (the incandescent type) electr作者: craving 時間: 2025-3-29 23:38 作者: 硬化 時間: 2025-3-29 23:57 作者: 緩和 時間: 2025-3-30 07:52
STarMir Tools for Prediction of microRNA Binding Sites,s an implementation of logistic prediction models developed with high-throughput miRNA binding data from cross-linking immunoprecipitation (CLIP) studies. The models incorporated comprehensive thermodynamic, structural, and sequence features, and were found to make improved predictions of both seed 作者: d-limonene 時間: 2025-3-30 09:59
Mapping RNA Structure In Vitro with SHAPE Chemistry and Next-Generation Sequencing (SHAPE-Seq),can then be used to infer RNA structures by restraining RNA structure prediction algorithms. Here, we provide a state-of-the-art protocol describing how to perform in vitro RNA structure probing with SHAPE chemistry using next-generation sequencing to quantify cDNA pools and estimate reactivities (S作者: 襲擊 時間: 2025-3-30 16:11 作者: glamor 時間: 2025-3-30 20:02
RNA 3D Structure Modeling by Combination of Template-Based Method ModeRNA, Template-Free Folding wit overcomes major limitations by combining two complementary approaches: template-based modeling that is capable of predicting global architectures based on similarity to other molecules but often fails to predict local unique features, and template-free modeling that can predict the local folding, 作者: dyspareunia 時間: 2025-3-30 21:01
NMR Methods for Characterization of RNA Secondary Structure, and sample considerations that can maximize data quality are discussed along with some details for optimal data acquisition and processing parameters. Approaches for identifying base pair types in both unlabeled and isotopically labeled RNA are covered. Common problems, such as missing signals and 作者: discord 時間: 2025-3-31 01:12
The Quick and the Dead: A Guide to Fast Phasing of Small Ribozyme and Riboswitch Crystal Structuresction (SAD or MAD) phasing. Test cases include an autolytic 62-mer RNA enzyme known as the hairpin ribozyme, and a 33-mer riboswitch that binds the modified guanine metabolite preQ.. The crystals were derivatized with iridium (III) hexammine and osmium (III) pentaammine triflate, respectively. Each 作者: CHOIR 時間: 2025-3-31 06:25
at transfer techniques. This has been especially true in the evolution of electronics packaging for digital computers. Since the development of the first electronic digital computers in the 1940s, the removal of heat has played a major role in ensuring their reliable operation. Early digital compute作者: 弄臟 時間: 2025-3-31 12:32 作者: cataract 時間: 2025-3-31 16:06 作者: 積習(xí)已深 時間: 2025-3-31 17:55
Zhenjiang Zech Xu,David H. Mathewseader will learn the latest ideas for reducing the scale of .In electronics manufacture, the expanding range of products and the smaller-and-smaller scale of increasingly integrated components is producing a trend towards complex and fault-susceptible processes. This fact, coupled with shorter produ作者: 能量守恒 時間: 2025-4-1 00:28
Laura DiChiacchio,David H. Mathewseader will learn the latest ideas for reducing the scale of .In electronics manufacture, the expanding range of products and the smaller-and-smaller scale of increasingly integrated components is producing a trend towards complex and fault-susceptible processes. This fact, coupled with shorter produ
