標(biāo)題: Titlebook: RNA Silencing; Methods and Protocol Gordon G. Carmichael Book 2005 Humana Press 2005 DNA.Vivo.gene expression.genes.molecular biology.regul [打印本頁] 作者: Colossal 時(shí)間: 2025-3-21 17:06
書目名稱RNA Silencing影響因子(影響力)
書目名稱RNA Silencing影響因子(影響力)學(xué)科排名
書目名稱RNA Silencing網(wǎng)絡(luò)公開度
書目名稱RNA Silencing網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱RNA Silencing被引頻次
書目名稱RNA Silencing被引頻次學(xué)科排名
書目名稱RNA Silencing年度引用
書目名稱RNA Silencing年度引用學(xué)科排名
書目名稱RNA Silencing讀者反饋
書目名稱RNA Silencing讀者反饋學(xué)科排名
作者: Dappled 時(shí)間: 2025-3-21 23:08 作者: 哺乳動物 時(shí)間: 2025-3-22 02:42 作者: Bumptious 時(shí)間: 2025-3-22 06:17 作者: antiandrogen 時(shí)間: 2025-3-22 09:12
siRNA Delivery In Vivo,RNAs) (.–.). This is now driven predominantly by siRNAs, as they are not technically demanding as traditional antisense and ribozyme technologies (.,.). The success of siRNAs as therapeutics, however, is largely dependent on the development of a delivery vehicle that can efficiently deliver them in vivo.作者: 核心 時(shí)間: 2025-3-22 15:39 作者: opprobrious 時(shí)間: 2025-3-22 17:27 作者: 難管 時(shí)間: 2025-3-22 21:12 作者: POWER 時(shí)間: 2025-3-23 03:30
Nathaniel R. Dudley,Bob Goldstein (.MLCT) excited state is lowest in energy. The pronounced solvatochromic and rigidochromic effects of the Ir. compounds are responsible for the reversal of the order of the lowest excited states. Mixing between the π-π* and MLCT excited states is reflected in the oscillator strengths, luminescence 作者: 虛弱 時(shí)間: 2025-3-23 07:22 作者: CHANT 時(shí)間: 2025-3-23 11:10
M. Florian Mette,Werner Aufsatz,Tatsuo Kanno,Lucia Daxinger,Philipp Rovina,Marjori Matzke,Antonius J-selective, and line-narrowed spectra of chromophores doped into matrices; the interplay between states of ligand-centered .ππ* and .MLCT character; localization versus delocalization behavior; radiative decay properties; zero-field splittings; spin-lattice relaxations via direct and Orbach mechanis作者: 使乳化 時(shí)間: 2025-3-23 15:59 作者: transplantation 時(shí)間: 2025-3-23 20:35
Peng Liu,Mary Louise Stover,Alexander Lichtler,David W. Roweengineering tools for improving pattern control and array efficiency including lattice selection, subarrray technology and pattern synthesis. Equations and figurers quantify the phenomena being described and provide the reader with the tools to tradeoff various performance features. The discussions 作者: 我怕被刺穿 時(shí)間: 2025-3-24 00:37
Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method,nctions by an endogenous pathway important for normal development in many organisms, including gene regulation, virus resistance, and chromatin remodeling (.–.). Although some components of the RNAi cellular machinery have been identified, the overall picture is far from clear. We describe the tande作者: 心胸狹窄 時(shí)間: 2025-3-24 05:32
Down-Regulating Gene Expression by RNA Interference in ,, epitope tagging can also be effected through homologous recombination (.). For general reviews of the peculiarities of trypanosome gene expression and RNA processing, see .. and ., and for pre-RNAi methods, such as inducible gene expression and knockouts by homologous recombination, see ... A few r作者: surrogate 時(shí)間: 2025-3-24 08:11
Inhibition of Gene Expression In Vivo Using Multiplex siRNA,ate the various phases of angiogenesis. A major regulator of angiogenesis, vascular endothelial growth factor (VEGF), was upregulated in tumor angiogenesis, and inhibition of this factor with a specific monoclonal antibody was recently approved for clinical use in colorectal cancer (.). However, ang作者: IRATE 時(shí)間: 2025-3-24 10:43
Peptide-Based Strategy for siRNA Delivery into Mammalian Cells, cells and in vivo (.). So far, although siRNA transfection can be achieved with classical laboratory-cultured cell lines using lipid-based formulations, siRNA delivery remains a major challenge for many cell lines and there is still no reasonably efficient method for in vivo application (.). The mo作者: 有斑點(diǎn) 時(shí)間: 2025-3-24 17:17 作者: 大門在匯總 時(shí)間: 2025-3-24 20:21 作者: calorie 時(shí)間: 2025-3-25 01:30 作者: 環(huán)形 時(shí)間: 2025-3-25 04:18 作者: Infraction 時(shí)間: 2025-3-25 08:16
Marianthi Kiriakidou,Peter Nelson,Stella Lamprinaki,Anup Sharma,Zissimos Mourelatosdemics, and enthusiasts seeking a comprehensive understanding of magnetic properties and critical phenomena within diverse spinel materials.978-3-031-40612-6978-3-031-40613-3Series ISSN 2192-1091 Series E-ISSN 2192-1105 作者: 抗生素 時(shí)間: 2025-3-25 12:18
1064-3745 uction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.978-1-61737-568-2978-1-59259-935-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 密碼 時(shí)間: 2025-3-25 16:59
RNA Interference in ,,systemic in that the silencing can spread between tissues throughout the adult as well as its progeny (.,.,.). Many neurons, however, are refractory to the spreading effect in wild-type backgrounds (.).作者: 不可比擬 時(shí)間: 2025-3-25 20:16 作者: 顯示 時(shí)間: 2025-3-26 00:54
Gene Silencing by a DNA Vector-Based RNAi Technology, other proteins. The siRNA guides the RISC to the homologous mRNA. Finally, the endoribonuclease in the RISC cleaves the targeted mRNA at the vicinity of the binding site, followed by further degradation of the mRNA by an exoribonuclease. As a result, gene silencing is established.作者: Concomitant 時(shí)間: 2025-3-26 04:24
Separation of , RNA Silencing Complexes by Native Gel Electrophoresis,holds promise for additional mechanistic studies, particularly those that are limited by sample size [such as experiments with mutant lysates (.)]. In this chapter, we describe the procedures involved in reproducibly detecting . siRNA/protein complexes and discuss significant issues as well as techniques for optimization.作者: ABASH 時(shí)間: 2025-3-26 10:08
Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silenche genome, such as DNA cytosine (C) methylation and histone methylation (.). This chapter focuses on a specific aspect of the third type of silencing: RNA-directed promoter methylation and transcriptional gene silencing (TGS).作者: 性行為放縱者 時(shí)間: 2025-3-26 14:26 作者: 變形詞 時(shí)間: 2025-3-26 17:06
Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietiof RNAi is required, such as in human immunodeficiency virus (HIV)-1-infected individuals. Lentiviral vectors are able to transduce nondividing cells, with sustained long-term expression of genes contained in the vector backbone.作者: 中子 時(shí)間: 2025-3-27 00:47 作者: 滲透 時(shí)間: 2025-3-27 02:43 作者: SEVER 時(shí)間: 2025-3-27 09:10
Silencing Gene Expression with Dicer-Generated siRNA Pools,. Binding of the antisense strand to the target mRNA triggers cleavage of the mRNA (.). siRNA-mediated gene silencing is very specific, most likely because of the high specificity of nucleotide base-pairing.作者: AWL 時(shí)間: 2025-3-27 11:36
High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes,nd . as posttranscriptional negative regulators for several genes, including ., ., and . genes (.). In general miRNAs bind to the 3′ untranslated regions (UTRs) of their target mRNAs with imperfect homology (.).作者: 移動 時(shí)間: 2025-3-27 14:11 作者: Infinitesimal 時(shí)間: 2025-3-27 20:56
Separation of , RNA Silencing Complexes by Native Gel Electrophoresis,xes and their precursors can often be resolved and analyzed by native gel electrophoresis. Although other techniques exist for large-scale separations, native gels require very little sample, making them superior for analytical applications. We have developed a native gel system for rapidly and reli作者: 有機(jī)體 時(shí)間: 2025-3-27 23:59 作者: 貧窮地活 時(shí)間: 2025-3-28 05:47
RNA Interference in ,,. This RNA-mediated interference, or RNAi, effectively shuts down expression of the target gene and can phenocopy loss-of-function mutations. RNAi is also remarkably potent, requiring only substoichiometric amounts of dsRNA to elicit a response (.). Another notable aspect of RNAi in . is that it is 作者: PACT 時(shí)間: 2025-3-28 08:22 作者: 600 時(shí)間: 2025-3-28 11:47 作者: glomeruli 時(shí)間: 2025-3-28 16:00
Strategies for the Design of Random siRNA Libraries and the Selection of anti-GFP siRNAs,nown, small, interfering RNAs (siRNAs) have been considered ideal for functional analysis and gene target validation (.–.). Furthermore, the technology has unprecedented target flexibility. By performing RNAi on a large scale using siRNA libraries, this reverse-genetic method can essentially be used作者: effrontery 時(shí)間: 2025-3-28 22:13 作者: 侵略主義 時(shí)間: 2025-3-29 02:17
Inhibition of Gene Expression In Vivo Using Multiplex siRNA,velopment, the physiological wound healing process, and the female menstrual cycle, blood vessel growth is coordinated concomitantly with tissue growth. However, during pathological processes such as solid cancer development, diabetic retinopathy, rheumatoid arthritis, and psoriasis, abnormal angiog作者: 改進(jìn) 時(shí)間: 2025-3-29 05:54
Gene Silencing by a DNA Vector-Based RNAi Technology, an RNase III-like dsRNA-specific endonuclease, Dicer, cleaves long dsRNA into 21- to 23-nucleotide (nt) small, interfering RNAs (siRNAs). Second, each resulting siRNA is incorporated into an RNA-induced silencing complex (RISC), which consists of eIF2C1, eIF2C2, Gemin3 (an RNA helicase), Gemin4 and作者: CRUE 時(shí)間: 2025-3-29 09:30 作者: critic 時(shí)間: 2025-3-29 13:52
siRNA Delivery In Vivo,RNAs) (.–.). This is now driven predominantly by siRNAs, as they are not technically demanding as traditional antisense and ribozyme technologies (.,.). The success of siRNAs as therapeutics, however, is largely dependent on the development of a delivery vehicle that can efficiently deliver them in 作者: Mobile 時(shí)間: 2025-3-29 16:15
Peptide-Based Strategy for siRNA Delivery into Mammalian Cells,very of the RNA interference pathway in a wide variety of eukaryotic organisms has provided a novel means of characterizing gene function in mammalian cells and new perspectives in both molecular biology and future therapeutic developments (.–.). Short, interfering RNAs (siRNAs) constitute a powerfu作者: 許可 時(shí)間: 2025-3-29 22:34
Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietiis a useful tool for studying gene function and regulation, but it also has potential for therapeutic applications. In many cases, a long-term effect of RNAi is required, such as in human immunodeficiency virus (HIV)-1-infected individuals. Lentiviral vectors are able to transduce nondividing cells,作者: 驕傲 時(shí)間: 2025-3-30 01:16 作者: 損壞 時(shí)間: 2025-3-30 06:51 作者: Bronchial-Tubes 時(shí)間: 2025-3-30 09:11
Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro,ecursor RNAs (pre-miRNAs) (.–.). miRNAs bind to Argonaute (Ago) proteins, such as Ago-2 (also known as eIF2C2) (.,.), and typically associate with additional proteins to form microribonucleoproteins (miRNPs) (.). Another class of approx 22-nt RNAs, termed short, interfering RNAs (siRNAs), is functio作者: Postulate 時(shí)間: 2025-3-30 13:08 作者: Preserve 時(shí)間: 2025-3-30 17:51 作者: FLEET 時(shí)間: 2025-3-30 23:11
Book 2005chniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, 作者: neutral-posture 時(shí)間: 2025-3-31 01:45 作者: 蒼白 時(shí)間: 2025-3-31 07:23 作者: Condescending 時(shí)間: 2025-3-31 09:21 作者: invulnerable 時(shí)間: 2025-3-31 14:19
Christine E. Clayton,Antonio M. Estévez,Claudia Hartmann,Vincent P. Alibu,Mark Field,David Hornolved and time-resolved emission and excitation spectra of per-protonated, per-deuterated, and partially deuterated [Pt(bpy).]., [Rh(bpy).]., [Ru(bpy).]., and [Os(bpy).].. Emphasis is placed on the use of the enormous amount of information displayed in well-resolved vibrational satellite structures.作者: investigate 時(shí)間: 2025-3-31 20:34
M. Florian Mette,Werner Aufsatz,Tatsuo Kanno,Lucia Daxinger,Philipp Rovina,Marjori Matzke,Antonius Jolved and time-resolved emission and excitation spectra of per-protonated, per-deuterated, and partially deuterated [Pt(bpy).]., [Rh(bpy).]., [Ru(bpy).]., and [Os(bpy).].. Emphasis is placed on the use of the enormous amount of information displayed in well-resolved vibrational satellite structures.作者: CREST 時(shí)間: 2025-3-31 22:50
Marianthi Kiriakidou,Peter Nelson,Stella Lamprinaki,Anup Sharma,Zissimos Mourelatosrstanding of critical phenomena and magnetic order.Presents This book explores magnetic properties and critical temperatures in inverse ferrite Fe??(M??Fe??)O? spinels (e.g., Fe, Co, Ni). It calculates transition and Curie Weiss temperatures, providing insights into their thermodynamic behavior. Usi作者: AGATE 時(shí)間: 2025-4-1 02:30
Mouldy Sioud,?ystein R?sok method, are performed to investigate both electronic and magnetic properties of the Fe.O.. Polarized spin and spin–orbit coupling are included in calculations within the framework of the antiferromagnetic state between two adjacent Fe plans. The antiferromagnetic and ferromagnetic energies of Fe.O.作者: Microgram 時(shí)間: 2025-4-1 08:25 作者: Euthyroid 時(shí)間: 2025-4-1 12:23
Einleitung,wesentliches Merkmal des Stahles die H?rtbarkeit angesehen. Es gibt aber eine ganze Reihe von St?hlen, die sich nicht h?rten lassen, die durch das Abschrecken aus hohen Temperaturen im Gegenteil sogar weicher, z?her werden.作者: Infantry 時(shí)間: 2025-4-1 16:25
Book 2011 doch als eines der wichtigsten Motivationsinstrumente für Mitarbeiter. Andreas Hack bietet dem Leser einen umfassenden überblick über die wissenschaftlichen Erkenntnisse zur Motivation von Mitarbeitern aus ?konomischer und psychologischer Perspektive. In einer umfangreichen empirischen Analyse bele
