標(biāo)題: Titlebook: RNA Scaffolds; Methods and Protocol Luc Ponchon Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2021 [打印本頁(yè)] 作者: ATE 時(shí)間: 2025-3-21 19:59
書目名稱RNA Scaffolds影響因子(影響力)
書目名稱RNA Scaffolds影響因子(影響力)學(xué)科排名
書目名稱RNA Scaffolds網(wǎng)絡(luò)公開(kāi)度
書目名稱RNA Scaffolds網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書目名稱RNA Scaffolds被引頻次
書目名稱RNA Scaffolds被引頻次學(xué)科排名
書目名稱RNA Scaffolds年度引用
書目名稱RNA Scaffolds年度引用學(xué)科排名
書目名稱RNA Scaffolds讀者反饋
書目名稱RNA Scaffolds讀者反饋學(xué)科排名
作者: 公式 時(shí)間: 2025-3-21 22:49
https://doi.org/10.1007/978-1-0716-1499-0Hammerhead Ribozymes; Aptazyme-Based Riboswitches; RNA Mango aptamers; In-Cell SHAPE; RNA-protein intera作者: 喃喃訴苦 時(shí)間: 2025-3-22 03:30
Luc PonchonIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 漫步 時(shí)間: 2025-3-22 06:53
978-1-0716-1501-0Springer Science+Business Media, LLC, part of Springer Nature 2021作者: Pulmonary-Veins 時(shí)間: 2025-3-22 10:14
RNA Scaffolds978-1-0716-1499-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 極小量 時(shí)間: 2025-3-22 14:13 作者: pus840 時(shí)間: 2025-3-22 20:19 作者: Frequency 時(shí)間: 2025-3-23 00:36
Jaehyung Lee,Andrew C. Keates,Chiang J. Lil for companies. This book should be a good resource for scholars and practitioners that need to understand the pervasive effects of eWOM..978-3-319-52458-0978-3-319-52459-7Series ISSN 2191-5482 Series E-ISSN 2191-5490 作者: 共棲 時(shí)間: 2025-3-23 03:31
1064-3745 .Cutting-edge and comprehensive, .RNA Scaffolds: Methods and Protocols, Second Edition. is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field.?.978-1-0716-1501-0978-1-0716-1499-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 背帶 時(shí)間: 2025-3-23 06:54 作者: acetylcholine 時(shí)間: 2025-3-23 11:57
Xiaojun Xu,Shi-Jie Chenments. More specifically, based on these two case studies it analyses four different challenges on the processing of personal data in remote electronic voting under the GDPR: the definitions and categories of personal data processed in online voting projects; the separation of duties between data co作者: 租約 時(shí)間: 2025-3-23 16:17
Grégoire De Bisschop,Bruno Sargueil the hearsay rule..In ascribing weight to the admitted CEEM, this paper proposes a standard that is based on the Relative Plausibility Theory; this standard will ensure that parties’ evidence is fairly evaluated and that winners are not made into losers in the courts.作者: Glaci冰 時(shí)間: 2025-3-23 20:07 作者: Gratulate 時(shí)間: 2025-3-23 22:56
Changrui Lu,Rujie Cai,Jason C. Grigg,Ailong Keaudits can improve the efficiency of the manual count; (3) that practical and organizational challenges must be overcome to keep audits repeatable, in particular it must be possible to identify individual ballots repeatedly and reliably; (4) it is possible to arrange an audit for the result of an ea作者: Adjourn 時(shí)間: 2025-3-24 05:45
Konstantin R?der,Samuela Pasqualistraints. The primary benefit is that ballot papers no longer pose a privacy risk. The solution has the major benefit of resolving the conflict between auditability and forward secrecy of printers, a problem left open by the most recent work in this area. Additional benefits include practical privac作者: 惰性女人 時(shí)間: 2025-3-24 09:17
Margot El Khouri,Marjorie Catala,Bili Seijo,Johana Chabal,Frédéric Dardel,Carine Tisné,Luc Ponchonith . dependence on transformations of the votes can easily be transformed to canonical form for SHANGRLA. We illustrate the approach by constructing assertions for party-list elections such as Hamiltonian free list elections and elections using the D’Hondt method, expanding the set of social choice作者: 抗體 時(shí)間: 2025-3-24 14:11 作者: 有特色 時(shí)間: 2025-3-24 14:54
José-Antonio Daròse United States slowed significantly. The 2002 federal and state elections took place without a trace of consideration for Internet voting, and the United States no longer occupies the ‘leading edge’ in the use of Internet technology for voting. In fact, the United States has fallen behind many Euro作者: Hectic 時(shí)間: 2025-3-24 19:46 作者: 大范圍流行 時(shí)間: 2025-3-25 00:31 作者: 案發(fā)地點(diǎn) 時(shí)間: 2025-3-25 04:43
Qikun Yu,Ru Zheng,Manojkumar Narayanan,Mingxu Youe United States slowed significantly. The 2002 federal and state elections took place without a trace of consideration for Internet voting, and the United States no longer occupies the ‘leading edge’ in the use of Internet technology for voting. In fact, the United States has fallen behind many Euro作者: GIDDY 時(shí)間: 2025-3-25 08:59 作者: 悄悄移動(dòng) 時(shí)間: 2025-3-25 15:24
Yoko Nomura,Yohei Yokobayashineously. Developed?new WPCB recycling?methods should take into account industrial applicability potential, automation in pretreatment, reducing processes and reagents, and paying more attention to nonmetallic fraction. WPCBs should be regarded as important secondary material sources for achieving hi作者: CODA 時(shí)間: 2025-3-25 16:47 作者: 消息靈通 時(shí)間: 2025-3-25 21:07 作者: stratum-corneum 時(shí)間: 2025-3-26 00:52 作者: Trigger-Point 時(shí)間: 2025-3-26 04:55 作者: inflame 時(shí)間: 2025-3-26 09:01 作者: 錫箔紙 時(shí)間: 2025-3-26 13:16
,FRET Analysis of RNA –Protein Interactions Using Spinach Aptamers,r this, the Spinach-DFHBI aptamer as RNA fusion and the red fluorescent mCherry as protein fusion was used..The methods presented here do not require any chemical modification of proteins or RNAs which make them relatively easy to use and to adopt on other systems. As all fluorophores are fusion tag作者: 姑姑在炫耀 時(shí)間: 2025-3-26 18:54
Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics,ic biology and clinical success in cancer immunotherapy provide renewed momentum for developing bacteria-based cancer immunotherapy for cancer treatment and should allow greater potential for the development of novel therapeutic approaches for this devastating disease.作者: 緩解 時(shí)間: 2025-3-27 00:33 作者: 空氣傳播 時(shí)間: 2025-3-27 01:15
RNA Footprinting Using Small Chemical Reagents, differences that are known as the ligand “footprint.” Such footprints allow for the identification of the precise site of the ligand interaction, but also reveals?RNA structural rearrangement upon ligand binding. Here we provide an experimental and analytical workflow to carry RNA footprinting experiments.作者: micronized 時(shí)間: 2025-3-27 05:33
Predicting RNA Scaffolds with a Hybrid Method of Vfold3D and VfoldLA,computational studies for RNA structures, at both the two-dimensional and the three-dimensional levels, led to several highly promising new developments. In this chapter, we describe a hybrid method, which combines the motif template-based Vfold3D model and the loop template-based VfoldLA model, to 作者: Admonish 時(shí)間: 2025-3-27 13:19
RNA Footprinting Using Small Chemical Reagents,onal structures that may form specific binding sites not only for proteins but for all sorts of molecules. Since the early days of molecular biology, strategies to probe RNA structure have been developed. Such probes are small molecules or RNases that most of the time specifically react with single 作者: 山頂可休息 時(shí)間: 2025-3-27 17:42
Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment,d viral RNAs. Here, we describe a protocol that combines postcrystallization dehydration and ion replacement that dramatically improved the diffraction quality of crystals of a large gene-regulatory tRNA–mRNA complex. Through this method, the resolution limit of X-ray data extended from 8.5 to 3.2??作者: 主動(dòng)脈 時(shí)間: 2025-3-27 20:08 作者: 形容詞 時(shí)間: 2025-3-27 23:03
RNA Modeling with the Computational Energy Landscape Framework,of RNA molecules at an atomic level of detail. As RNA molecules are known to adopt multiple conformations with comparable energies, but different two-dimensional structures, all-atom models are necessary to better describe the structural ensembles for RNA molecules. This point is important because d作者: Cacophonous 時(shí)間: 2025-3-28 05:01
,Coexpression and Copurification of RNA–Protein Complexes in , that allows for efficient in vivo expression of recombinant RNA in .. We have extended the “tRNA scaffold” method to RNA–protein coexpression in order to express and purify RNA by affinity in native condition. As a proof of concept, we present the expression and the purification of the AtRNA-mala i作者: Implicit 時(shí)間: 2025-3-28 06:20
In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold,itro. Herein, we describe an alternative approach in which RNAs of interest are expressed as a fusion with a 5S rRNA-derived scaffold. The scaffold provides protection against cellular ribonucleases resulting in cellular accumulations comparable to those of regular ribosomal RNAs. After isolation of作者: AVOID 時(shí)間: 2025-3-28 10:38
Production of Circular Recombinant RNA in , Using Viroid Scaffolds,ery stable scaffolds to produce recombinant RNA in .. Coexpression of an RNA precursor consisting of a viroid monomer, in which the RNA of interest is inserted, flanked by domains of the viroid hammerhead ribozyme, along with a host plant tRNA ligase, the enzyme that catalyzes viroid circularization作者: 駁船 時(shí)間: 2025-3-28 17:23 作者: 碎石頭 時(shí)間: 2025-3-28 20:37 作者: 協(xié)迫 時(shí)間: 2025-3-29 00:38
Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging,ly used for the cellular imaging, but the choice of targets is still limited. Recently, fluorogenic RNA-based sensors gained lots of attention. This novel sensor system can function as a general platform for various cellular targets. Here, we describe the steps to rationally design, optimize, and ap作者: 星球的光亮度 時(shí)間: 2025-3-29 05:57 作者: 楓樹(shù) 時(shí)間: 2025-3-29 09:18
,FRET Analysis of RNA –Protein Interactions Using Spinach Aptamers,ery of fluorescent light-up aptamers (FLAPs), short RNA aptamers that switch the fluorescence of small, cell-permeable, and nontoxic organic chromophores on, paved the road for their utilization in direct RNA–protein interactions. The combination with fluorescent proteins as biological fluorophores 作者: 斗志 時(shí)間: 2025-3-29 15:15
Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vi aptamer domain and a self-cleaving ribozyme. The utilization of aptazymes for conditional gene expression displays several advantages over employing conventional transcription factor-based techniques as aptazymes require minimal genomic space, fulfill their function without the need of protein cofa作者: detach 時(shí)間: 2025-3-29 15:51
Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells,itch libraries are constructed by randomizing the key nucleotides that couple the molecular recognition function of an aptamer with the self-cleavage activity of a ribozyme. The allosteric ribozyme (aptazyme) candidates are cloned in the 3′ untranslated region (UTR) of a reporter gene mRNA. The plas作者: inclusive 時(shí)間: 2025-3-29 23:37
,Folding RNA–Protein Complex into Designed Nanostructures,otifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nanoobjects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we desc作者: Lymphocyte 時(shí)間: 2025-3-29 23:57 作者: Biguanides 時(shí)間: 2025-3-30 05:02 作者: 阻擋 時(shí)間: 2025-3-30 08:40 作者: 偏離 時(shí)間: 2025-3-30 13:06 作者: 善辯 時(shí)間: 2025-3-30 19:52 作者: 輕而薄 時(shí)間: 2025-3-30 22:32 作者: 萬(wàn)神殿 時(shí)間: 2025-3-31 04:38
Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria,the riboswitch. This method describes how reporters for live cell imaging, such as yellow fluorescent protein (YFP), can be placed under genetic control by metabolite-sensing riboswitches in the bacterium .. Specifically, a protocol for generating a fluorescent YFP reporter, based on a c-di-GMP responsive riboswitch, is outlined below.作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-31 08:38
Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment,, enabling structure determination. Although this protocol was developed for a particular RNA complex, the general importance of solvent and counterions in nucleic acid structure may render it generally useful for crystallographic analysis of other RNAs.作者: BLA 時(shí)間: 2025-3-31 10:30
In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold, the chimeric RNA from the cells, the scaffold can be removed, if necessary, by deoxyribozyme-catalyzed cleavage followed by preparative electrophoretic separation of the reaction products. The protocol is designed for sustained production of high quality RNA on the milligram scale.作者: critic 時(shí)間: 2025-3-31 15:10
Identification of RNA-Binding Proteins Associated to RNA Structural Elements,nalysis to identify novel interactors recognizing RNA structural domains. This approach takes advantage of tRNA-scaffold guided expression, allowing the identification of factors belonging to networks involved in RNA and protein metabolism.作者: ventilate 時(shí)間: 2025-3-31 21:18 作者: 柏樹(shù) 時(shí)間: 2025-3-31 23:37
Predicting RNA Scaffolds with a Hybrid Method of Vfold3D and VfoldLA,e assembly from templates of motifs and loops. For illustration, we use the ZIKV xrRNA1 as an example to show the template-based prediction of RNA 3D structures from the 2D structure. The web server for the hybrid model is freely accessible at ..作者: 粘土 時(shí)間: 2025-4-1 02:02
Production of Circular Recombinant RNA in , Using Viroid Scaffolds,ccumulation most probably results from viroid scaffold stability, resistance to exonucleases due to circularity, and accumulation as a ribonucleoprotein complex with tRNA ligase. Purification of the recombinant RNA from total . RNA is also facilitated by the circular structure of the product.作者: ICLE 時(shí)間: 2025-4-1 07:42 作者: 黃油沒(méi)有 時(shí)間: 2025-4-1 10:45
Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells,zymes can be cloned into the 3′ UTR of a desired gene to obtain a logic gate response to multiple chemical signals. This screening strategy complements other methods to engineer robust mammalian riboswitches to control gene expression.
