標(biāo)題: Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2021Latest edition Springer Science+Business Media, LLC, part of Spring [打印本頁] 作者: Fixate 時間: 2025-3-21 18:47
書目名稱RNA Remodeling Proteins影響因子(影響力)
作者: Transfusion 時間: 2025-3-21 21:37
1064-3745 ation advice from the experts.This volume.?.provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex 作者: RENAL 時間: 2025-3-22 01:51 作者: excrete 時間: 2025-3-22 07:05
Happy Birthday: 30 Years of RNA Helicases,y the structure of RNA molecules and/or the functionality of ribonucleoprotein complexes. Ultimately, the action of RNA helicases results in changes in gene expression that allow the cell to perform crucial functions. In this chapter, we review established and emerging concepts for DEAD-box and DExH作者: LURE 時間: 2025-3-22 12:16 作者: 合乎習(xí)俗 時間: 2025-3-22 13:54 作者: senile-dementia 時間: 2025-3-22 17:04
Thermal Shift Assay for Characterizing the Stability of RNA Helicases and Their Interaction with Liof various ligands on that stability. It is particularly useful for the assaying of ligands that may stabilize oligomeric helicases, which rely on both substrates (oligonucleotides) and nucleotide cofactors (ATP analogues) for their stability in a functional state. In this chapter, we describe the r作者: crease 時間: 2025-3-22 22:25 作者: BRUNT 時間: 2025-3-23 01:30
,High-Throughput Protein–Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhanchese interactions is crucial for delineating the mechanisms governing transcription, genome duplication, and translation. Here we describe a detailed protocol for the quantitative analysis of protein–nucleic acid interactions based on protein-induced fluorescence enhancement (PIFE). While PIFE has m作者: 動物 時間: 2025-3-23 09:17
Probing RNA Helicase Conformational Changes by Single-Molecule FRET Microscopy,nd total internal reflection fluorescence (TIRF) microscopy to follow the conformational cycling of DEAD-box helicases on the single molecule level, using the eukaryotic translation initiation factor eIF4A as an illustrative example. Confocal microscopy enables the study of donor-acceptor-labeled mo作者: Deceit 時間: 2025-3-23 11:26
A Fluorescent Assay to Monitor Ligand-Dependent Closure of the Hexameric Rho Helicase Ring,ranscripts. During its catalytic cycle, Rho both loads onto and translocates along RNA through a series of tightly regulated, ligand-dependent conformational changes. Here we describe an assay to track Rho as it switches from an open-ring (RNA-loading) to a closed-ring (RNA-translocation) configurat作者: 用不完 時間: 2025-3-23 14:05 作者: LAPSE 時間: 2025-3-23 19:57 作者: Creatinine-Test 時間: 2025-3-23 23:11
Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activitignetic tweezers-based single-molecule investigation of the binding of helicases to G-quadruplex structures and their ATP-dependent unwinding activity, using DHX36 (also known as RHAU and G4R1) helicase and a DNA G-quadruplex structure for an example. We specifically emphasize on the principle and me作者: insecticide 時間: 2025-3-24 05:28
Characterization of the Brr2 RNA Helicase and Its Regulation by Other Spliceosomal Proteins Using Gg and unwinding assays. Here we describe detailed protocols for the production and purification of the spliceosomal Ski2-like RNA helicase, Brr2, and one of its regulatory factors, the Jab1 domain of the Prp8 protein from yeast. Furthermore, we include a production protocol for radioactively labeled作者: 碎石 時間: 2025-3-24 07:22
Monitoring Acetylation of the RNA Helicase DDX3X, a Protein Critical for Formation of Stress Granulhad previously shown that the lysine deacetylase HDAC6 is required for the formation of stress granules, but the substrate important for this function was not known. We recently found that the RNA helicase DDX3X is a novel HDAC6 substrate, which is critical for the formation of stress granules. Thro作者: BILE 時間: 2025-3-24 12:11 作者: Anthropoid 時間: 2025-3-24 18:53
Deciphering the Dynamic Landscape of Transcription-Associated mRNP Quality Control Components Over ir nuclear retention and ultimately the degradation of their mRNA component by the 3′–5′ activity of the exosome-associated exonuclease Rrp6. This mRNP quality control process is stimulated by the NNS complex (Nrd1-Nab3-Sen1), which otherwise mediates termination, processing, and decay of ncRNAs. Th作者: 慌張 時間: 2025-3-24 21:17 作者: conifer 時間: 2025-3-25 01:39
Probing Transcriptome-Wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2, proteins form the largest family in eukaryotes and have been shown to remodel RNA/RNP structures and clamping of RNA-binding proteins, both in vitro and in vivo. Nevertheless, for the majority of these enzymes, it is largely unclear what RNAs are targeted and where they modulate RNA/RNP structures 作者: 松軟無力 時間: 2025-3-25 03:50 作者: Vertical 時間: 2025-3-25 11:00 作者: 媒介 時間: 2025-3-25 13:44
Probing RNA Helicase Conformational Changes by Single-Molecule FRET Microscopy,lecules in solution, revealing the population of different conformational states present. With TIRF microscopy, surface-immobilized molecules can be imaged as a function of time, revealing sequences of conformational states and the kinetics of conformational changes.作者: Allowance 時間: 2025-3-25 16:58 作者: SLUMP 時間: 2025-3-25 23:37 作者: figure 時間: 2025-3-26 01:15 作者: 石墨 時間: 2025-3-26 07:45 作者: 圖表證明 時間: 2025-3-26 09:47 作者: MOAN 時間: 2025-3-26 14:28
Identifying RNA Helicase Inhibitors Using Duplex Unwinding Assays,-throughput modalities. These optimized assays have enabled the application of rapid screening approaches to be applied toward discovering helicase inhibitors. This chapter provides detailed protocols for utilizing a medium to high-throughput approach for inhibitor discovery.作者: Concerto 時間: 2025-3-26 20:47
SRCD and FTIR Spectroscopies to Monitor Protein-Induced Nucleic Acid Remodeling,ynchrotron radiation circular dichroism (SRCD) and FTIR to analyze how proteins may change RNA structure. These include the analysis of RNA melting, or stabilization, of change in helical parameters and base stacking. The effects on the structure of RNA remodeling proteins are also presented.?作者: 其他 時間: 2025-3-27 00:01
A Simple Fluorescence Microplate Assay to Monitor RNA-DNA Hybrid Unwinding by the Bacterial Transcrelectrophoresis. Here, we describe a fluorescence-based microplate assay that informs on Rho helicase activity in a matter of minutes and allows the multiplexed analysis of conditions required for primary biochemical characterization or for drug screening.作者: FER 時間: 2025-3-27 01:06
Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activititing the DHX36-induced changes in the lifetime of the DNA G-quadruplex under tension. The principle of the measurement can be broadly extended to the studies of other DNA or RNA G-quadruplex helicases.作者: filicide 時間: 2025-3-27 08:03
Probing Transcriptome-Wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2,mes in vivo. In this chapter, we describe a protocol for identification of RNA structural changes that are dependent on a . DEAD-box helicase Dbp2. Experiments detailed here can be adapted to the study of other RNA helicases and identification of putative remodeling targets in vivo.作者: 難聽的聲音 時間: 2025-3-27 13:04 作者: 新陳代謝 時間: 2025-3-27 17:04 作者: PAEAN 時間: 2025-3-27 19:56 作者: Vulnerary 時間: 2025-3-27 23:17
Known Inhibitors of RNA Helicases and Their Therapeutic Potential,ases, the different types of compounds explored, the proposed inhibitory mechanisms of the compounds on the proteins, and the therapeutic potential of these compounds in the treatment of various disorders.作者: 改革運動 時間: 2025-3-28 05:49
,High-Throughput Protein–Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhancthroughput. These properties predispose mwPIFE to become a method of choice for routine applications that require multiple parallel measurements such as buffer optimization, competition experiments, or screening chemical libraries for binding modulators.作者: HEPA-filter 時間: 2025-3-28 08:13 作者: 玩笑 時間: 2025-3-28 13:50 作者: 提升 時間: 2025-3-28 18:10 作者: 一再煩擾 時間: 2025-3-28 20:47 作者: cruise 時間: 2025-3-29 00:22
Emmanuel Saridakis,Franck Costen used for such diverse aspects of culture as airborne imagery, computer generated art based on the autoimmune system, motion capture for music and for sign language, the visualisation of time and the long term preservation of these materials. Selected from the EVA London conferences from 2009-2012,作者: Coronary 時間: 2025-3-29 03:46
Frank Wien,Frédéric Geinguenaud,Wilfried Grange,Véronique Arluisonn used for such diverse aspects of culture as airborne imagery, computer generated art based on the autoimmune system, motion capture for music and for sign language, the visualisation of time and the long term preservation of these materials. Selected from the EVA London conferences from 2009-2012,作者: 表兩個 時間: 2025-3-29 07:45
Michael R. Lawson,James M. Bergern used for such diverse aspects of culture as airborne imagery, computer generated art based on the autoimmune system, motion capture for music and for sign language, the visualisation of time and the long term preservation of these materials. Selected from the EVA London conferences from 2009-2012,作者: 易達到 時間: 2025-3-29 15:10
Ewan K. S. McRae,Steven J. Dupas,Negar Atefi,Sean A. McKenna outlined above, while providing stronger security guarantees. Our method does not increase the risk of vote selling compared to the underlying voting protocol to be augmented, and it requires only comparatively weak trust assumptions to achieve this. It can be combined with various voting protocols作者: 清真寺 時間: 2025-3-29 15:54 作者: 不知疲倦 時間: 2025-3-29 21:38 作者: commodity 時間: 2025-3-30 00:06
Makoto Saito,Vytautas Iestamantavicius,Daniel Hess,Patrick Matthiasy jurisdiction in every election—more voters than there are in most U.S. jurisdictions. (iii)?Even with a perfect model of voter behavior, the required number of tests exceeds the number of voters in most U.S. jurisdictions. (iv)?An attacker can target interactions that are intrinsically expensive t作者: 不怕任性 時間: 2025-3-30 04:19
Sarah F. Mitchellelines are expected to be completed through further work. The main differences between the old and the new Council of Europe standards on e-voting are outlined. Correlations are illustrated. The expected use, impact and evolution of the Recommendation and Guidelines are briefly explained.作者: debase 時間: 2025-3-30 11:51
Kévin Moreau,Aurélia Le Dantec,A. Rachid Rahmouniet. Researchers found critical errors in the source code of both individual and universal verifiability. The PIT revealed other, less critical issues. This experience has fuelled the already heated debate over the future development of internet voting in Switzerland. It questions the procedures for 作者: 厚臉皮 時間: 2025-3-30 12:44
Srivathsan Adivarahan,Daniel Zenklusenail to determine whether the result is trustworthy..We show how to apply two RLA strategies, . and ., to General Elections in India. Our main result is a novel method for combining RLAs in constituencies to obtain an RLA of the overall parliamentary election result.作者: nautical 時間: 2025-3-30 18:43
Yu-Hsuan Lai,Elizabeth J. Tranet. Researchers found critical errors in the source code of both individual and universal verifiability. The PIT revealed other, less critical issues. This experience has fuelled the already heated debate over the future development of internet voting in Switzerland. It questions the procedures for 作者: 潔凈 時間: 2025-3-30 22:57
Daniela Toro-Ascuy,Aracelly Gaete-Argel,Victoria Rojas-Celis,Fernando Valiente-Echeverriaure a large chance of correcting the outcome of an election when the reported outcome is wrong. “Nonpartisan” priors, i.e., priors that are invariant under relabeling the candidates, lead to upset probabilities that can be far smaller than the chance of correcting wrong reported outcomes. We demonst作者: nocturnal 時間: 2025-3-31 04:51
Yosser Zina Abdelkrim,Josette Banroques,N. Kyle Tanner作者: Lasting 時間: 2025-3-31 05:46 作者: 推遲 時間: 2025-3-31 12:28
Martina Valentini,Patrick Linders how “gateways” in transactional networks can reinforce or undermine established social boundaries, and reviews the establishment of trust in new payment devices..978-1-4471-2623-2978-1-84996-139-4Series ISSN 2190-6831 Series E-ISSN 2190-684X 作者: NADIR 時間: 2025-3-31 16:42
Jaroslav Fulne?ek,Karel ?íhaed on the autoimmune system, motion capture for music and for sign language, the visualisation of time and the long term preservation of these materials. Selected from the EVA London conferences from 2009-2012,978-1-4471-7235-2978-1-4471-5406-8Series ISSN 2195-9056 Series E-ISSN 2195-9064 作者: BILIO 時間: 2025-3-31 19:15
Linda Krause,Dagmar Klostermeiered on the autoimmune system, motion capture for music and for sign language, the visualisation of time and the long term preservation of these materials. Selected from the EVA London conferences from 2009-2012,978-1-4471-7235-2978-1-4471-5406-8Series ISSN 2195-9056 Series E-ISSN 2195-9064 作者: 小步舞 時間: 2025-3-31 23:36
Isabelle Simon,Marc Boudvillained on the autoimmune system, motion capture for music and for sign language, the visualisation of time and the long term preservation of these materials. Selected from the EVA London conferences from 2009-2012,978-1-4471-7235-2978-1-4471-5406-8Series ISSN 2195-9056 Series E-ISSN 2195-9064 作者: 倔強不能 時間: 2025-4-1 01:49
W. Luke Ward,Rick Russellarrative with sociological analysis, describing the economic.Electronic Value Exchange. examines in detail the transformation of the VISA electronic payment system from a collection of non-integrated, localized, paper-based bank credit card programs into the cooperative, global, electronic value exc
