標(biāo)題: Titlebook: RNA Methylation; Methods and Protocol Alexandra Lusser Book 2017 Springer Science+Business Media LLC 2017 posttranscriptional base modifica [打印本頁] 作者: 灰塵 時間: 2025-3-21 19:20
書目名稱RNA Methylation影響因子(影響力)
書目名稱RNA Methylation影響因子(影響力)學(xué)科排名
書目名稱RNA Methylation網(wǎng)絡(luò)公開度
書目名稱RNA Methylation網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱RNA Methylation被引頻次
書目名稱RNA Methylation被引頻次學(xué)科排名
書目名稱RNA Methylation年度引用
書目名稱RNA Methylation年度引用學(xué)科排名
書目名稱RNA Methylation讀者反饋
書目名稱RNA Methylation讀者反饋學(xué)科排名
作者: Type-1-Diabetes 時間: 2025-3-21 20:49 作者: Cytokines 時間: 2025-3-22 01:06
Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation determination of modified nucleosides in both DNA and RNA. Here, we describe a protocol to analyze m.A in RNA by LC-ESI-MS/MS. And this protocol also can be extended to the analysis of other modified nucleosides in both DNA and RNA.作者: muffler 時間: 2025-3-22 08:38
Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq).A positive RNA fragments are subsequently sequenced by RNA-seq in parallel with background control non-immunoprecipitated input RNA fragments. Analyses reveal peaks of m.A enrichment containing sites of modifications analogous to chromatin modification immunoprecipitation experiments.作者: fender 時間: 2025-3-22 08:46
Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencingissues. Although the nature of the bisulfite sequencing protocol makes it comparably easy to translate from a low to a high-throughput approach, several critical points require attention before starting such a project. We describe a step-by-step protocol for planning and performing the experiment and analyzing the data.作者: manifestation 時間: 2025-3-22 14:34
Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP) we describe how to use these mutational signatures to map m6A residues at nucleotide resolution. Taken together, our protocol allows for high-throughput detection of individual m6A residues throughout the transcriptome.作者: Gratulate 時間: 2025-3-22 19:37
Illustrating the Epitranscriptome at Nucleotide Resolution Using Methylation-iCLIP (miCLIP)thyl-6-adenosine (m6A) modification at nucleotide resolution in the human transcriptome. Here we describe the m5C-miCLIP protocol, discuss how it yields the nucleotide-resolution RNA modification maps, and comment on how these have contributed to the new field of molecular genetics research coined “epitranscriptomics.”作者: 愛好 時間: 2025-3-23 00:58
Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencingysis of cytosine methylation in low abundance poly(A)RNA using a combination of commercially available kits and standard lab methods to ensure reproducible results. Furthermore, useful information on optimizing the method, suitable controls for almost all steps, and general troubleshooting guides are provided.作者: dysphagia 時間: 2025-3-23 02:50
Book 2017focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-作者: 一小塊 時間: 2025-3-23 06:26
Detection and Quantification of ,,-Methyladenosine in Messenger RNA by TLCs often useful to directly measure and compare ..-methyladenosine levels between samples. Two dimensional chromatography of radiolabeled nucleotides, following specific nuclease treatments, provides a robust, sensitive, and reproducible assay for this modification.作者: Scintigraphy 時間: 2025-3-23 11:10
RiboMeth-seq: Profiling of 2′, in RNAes alkaline fragmentation and a specialized library construction protocol based on 5.-OH and 2.,3. cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2.-.-Me sites.作者: Nutrient 時間: 2025-3-23 16:33
High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)rotocols, resulting in inefficient detection of methyl-modified RNAs. Here, we describe a procedure to demethylate RNAs containing m.A, m.C, or m.G using the . dealkylating enzyme AlkB, along with instructions for subsequent processing with widely used protocols for small RNA sequencing.作者: Alveolar-Bone 時間: 2025-3-23 21:51
LC-MS Analysis of Methylated RNAation of gene expression, immune response, or epigenetic alterations. Therefore, it is necessary to have methods available, which are extremely sensitive and accurate, for instance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Here, we describe the preparation of RNA samples by enzymati作者: Glycogen 時間: 2025-3-24 02:16
Comparative Analysis of Ribonucleic Acid Digests (CARD) by Mass Spectrometry during RNase digestion, which allows the direct comparison of a tRNA of unknown modification status against a reference tRNA, whose sequence or modification status is known. The reference sample is labeled with .O during RNase digestion while the candidate (unknown) sample is labeled with .O. These作者: 殺人 時間: 2025-3-24 02:39 作者: 神經(jīng) 時間: 2025-3-24 08:50
Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq)It allows the study of the impact of various perturbations on m.A modification distribution and the study of m.A functions. Herein, we describe the m.A-seq protocol, which entails RNA immunoprecipitation (RIP) performed on fragmented poly(A) RNA utilizing anti-m.A antibodies. The captured/enriched m作者: 罵人有污點 時間: 2025-3-24 12:24 作者: dearth 時間: 2025-3-24 17:03
Detection and Quantification of ,,-Methyladenosine in Messenger RNA by TLCs can change dependent upon environmental conditions, cell differentiation state, or following knockdown of members of the methylase complex, and it is often useful to directly measure and compare ..-methyladenosine levels between samples. Two dimensional chromatography of radiolabeled nucleotides, 作者: Petechiae 時間: 2025-3-24 22:13 作者: LEERY 時間: 2025-3-25 01:04 作者: 斗爭 時間: 2025-3-25 04:40
Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencingmbination of bisulfite treatment of RNA with today’s high-throughput sequencing techniques opens the door to methylation studies at nucleotide resolution on a transcriptome-wide scale. Below we describe a protocol for the transcriptome-wide analysis of total or fractionated poly(A)RNA in cells and t作者: electrolyte 時間: 2025-3-25 10:25 作者: CHOIR 時間: 2025-3-25 11:49
High-Throughput Mapping of 2,-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMee physico-chemical methods require purification of the RNA of interest almost to homogeneity. To overcome these limitations, methods based on RT-driven primer extension have been developed and successfully used, sometimes in combination with a specific chemical treatment. Nowadays, some of these app作者: INCH 時間: 2025-3-25 16:29 作者: Brain-Imaging 時間: 2025-3-25 22:55 作者: 可以任性 時間: 2025-3-26 02:00
High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)difications alter Watson-Crick base-pairing, and cause pauses or stops during reverse transcription required for most high-throughput RNA sequencing protocols, resulting in inefficient detection of methyl-modified RNAs. Here, we describe a procedure to demethylate RNAs containing m.A, m.C, or m.G us作者: Acupressure 時間: 2025-3-26 04:40
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820180.jpg作者: 形容詞詞尾 時間: 2025-3-26 10:20
https://doi.org/10.1007/978-1-4939-6807-7posttranscriptional base modifications; N6-methyladenosine (m6A); 5-methyl cytosine (m5C); mRNAs; long n作者: itinerary 時間: 2025-3-26 13:15
Analysis of High-Throughput RNA Bisulfite Sequencing DataMethylation of the 5-cytosine (m.C) is a common but not well-understood RNA modification, which can be detected by sequencing of bisulfite-treated transcripts (RNA-BSseq). In this Chapter, we discuss computational RNA-BSseq data analysis methods for transcriptome-wide identification and quantification of m.C.作者: Chipmunk 時間: 2025-3-26 19:55 作者: Ceremony 時間: 2025-3-26 22:08 作者: Flirtatious 時間: 2025-3-27 04:42
978-1-4939-8304-9Springer Science+Business Media LLC 2017作者: Entropion 時間: 2025-3-27 05:54
RNA Methylation978-1-4939-6807-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 送秋波 時間: 2025-3-27 11:32 作者: nautical 時間: 2025-3-27 14:29
hing he/she is. Security is also achieved through a secure transmission process including the Internet such that a document signed through an electronic signature is not tampered with by a third person and reaches the recipient in the form in which it left the signatory.作者: Guileless 時間: 2025-3-27 18:28
Kathrin Thüring,Katharina Schmid,Patrick Keller,Mark Helmhat the severest deficits only occurred in those who had lacked visual experience in their earliest years. A spatial sensor that could supply relevant information, albeit through a different sense, seemed like an exciting possibility for remedying — if not fully redressing — some of the deficit. Giv作者: 斥責(zé) 時間: 2025-3-28 00:46 作者: 莊嚴(yán) 時間: 2025-3-28 02:44 作者: conifer 時間: 2025-3-28 08:16 作者: 現(xiàn)實 時間: 2025-3-28 14:22 作者: 清醒 時間: 2025-3-28 17:03 作者: 愛了嗎 時間: 2025-3-28 19:44
heory of boundary value problems for ordinary differential equations, the existence and locations of the zeros of the solutions of such equations are often of central importance. After reviewing some elementary knowledge on the theory of second-order linear ordinary differential equations, we introd作者: comely 時間: 2025-3-29 02:30
Harry George,Jernej Ule,Shobbir Hussainthematical theorem in [.], we show that in many simple but interesting cases, the properties of electronic states in ideal low-dimensional systems and finite crystals can be understood, how the energies of these electronic states depend on the size and/or the shape of the system can be predicted, an作者: Exposition 時間: 2025-3-29 03:40 作者: 免費 時間: 2025-3-29 07:35
Eva Hrabeta-Robinson,Erin Marcus,Aaron E. Cozen,Eric M. Phizicky,Todd M. Lowesemi-infinite one-dimensional crystals is developed. In addition, there are various revisions and improvements, including using the Kronig-Penney model to illustrate the analytical theory and make it easier to understand. This book is a valuable resource for solid-state physicists and material scien作者: 以煙熏消毒 時間: 2025-3-29 15:27 作者: indigenous 時間: 2025-3-29 17:49
High-Throughput Mapping of 2,-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMegy for detection and relative quantification of 2.-O-methylations with a high sensitivity and reproducibility even with a limited amount of starting material (1 ng of total RNA). Altogether this technique unlocks a technological barrier since it will be applicable for routine parallel treatment of b作者: vasculitis 時間: 2025-3-29 23:37 作者: Oligarchy 時間: 2025-3-30 01:54
Zsuzsanna Bodi,Rupert G. Frayool mates had the same feeling. Among many solid state physics books, the author found that only in the classic book Dynamic Theory of Crystal Lattices by Born 978-1-4419-2087-4978-0-387-26304-5Series ISSN 0081-3869 Series E-ISSN 1615-0430 作者: oxidize 時間: 2025-3-30 04:31 作者: headway 時間: 2025-3-30 11:13 作者: 哪有黃油 時間: 2025-3-30 15:29
1064-3745 Cutting-edge and thorough, .RNA Methylation: Methods and Protocols?.is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations...?.978-1-4939-8304-9978-1-4939-6807-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
