標(biāo)題: Titlebook: RNA Isolation and Characterization Protocols; Ralph Rapley,David L. Manning Book 1998 Springer Science+Business Media New York 1998 [打印本頁] 作者: Enkephalin 時(shí)間: 2025-3-21 16:15
書目名稱RNA Isolation and Characterization Protocols影響因子(影響力)
書目名稱RNA Isolation and Characterization Protocols影響因子(影響力)學(xué)科排名
書目名稱RNA Isolation and Characterization Protocols網(wǎng)絡(luò)公開度
書目名稱RNA Isolation and Characterization Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱RNA Isolation and Characterization Protocols被引頻次
書目名稱RNA Isolation and Characterization Protocols被引頻次學(xué)科排名
書目名稱RNA Isolation and Characterization Protocols年度引用
書目名稱RNA Isolation and Characterization Protocols年度引用學(xué)科排名
書目名稱RNA Isolation and Characterization Protocols讀者反饋
書目名稱RNA Isolation and Characterization Protocols讀者反饋學(xué)科排名
作者: Diatribe 時(shí)間: 2025-3-21 20:46
Nonradioactive Northern Blotting,nt of chemiluminescent substrates (e.g., CSPD, CPD, CPD-Star from Troptx) has improved the sensitivity of the detection to a range that was not achieved with calorimetric detection via chromogenic substrates (e.g., 5-chloro-4-bromo-indolylphosphate) for alkaline phosphatase.作者: Occupation 時(shí)間: 2025-3-22 04:04
Isolation of Total RNA from Tissues or Cell Lines, procedure involves four major steps: complete disruption of the cells or tissues; effective denaturation of the nuclear protein complex; inactivation of endogenous ribonuclease (RNase) activity; and purification of the RNA from the contaminating DNA, protein, and carbohydrates.作者: Astigmatism 時(shí)間: 2025-3-22 08:18
Formaldehyde Gel Electrophoresis of Total RNA,ently, the electrophoresis of RNA needs to be performed under denaturing conditions. Heat denaturing the RNA sample prior to electrophoresis is insufficient, as secondary structures will simply reform unless a denaturing system is used.作者: conspicuous 時(shí)間: 2025-3-22 12:07
1064-3745 olecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecule作者: forager 時(shí)間: 2025-3-22 15:59 作者: 令人不快 時(shí)間: 2025-3-22 20:46 作者: 保全 時(shí)間: 2025-3-22 22:17
An Improved Rapid Method of Isolating RNA from Cultured Cells,l RNA. Most protocols rely on sodium dodecyl sulfate (SDS) (.) or guamdium thiocyanate (.,.) to simultaneously lyse cells and mactivate endogenous ribonucleases. In those procedures, the RNA 1s separated from cellular DNA and proteins by centrifugation through 5.7 . CsC1, or by acid phenol extractio作者: conference 時(shí)間: 2025-3-23 02:22
Isolating RNA with the Cationic Surfactant, Catrimox-14,phase, and the cationic head groups bind to the nucleic acid electrostatically. The precipitate can be dissolved in organic solvents, or it can be redissolved in water by the addition of salt. Selected cationic surfactants are capable of lysing cells, by solubilizmg their protem and lipid components作者: 方便 時(shí)間: 2025-3-23 08:01 作者: 一大群 時(shí)間: 2025-3-23 10:38
Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides,eing a difficult source from which to isolate high-quality RNA with good yield. This difficulty is primarily due to the presence of naturally occurrmg polysaccharides and/or polyphenols that are released during cell disruption. These compounds form complexes with nucleic acids during tissue extracti作者: 不適 時(shí)間: 2025-3-23 15:56
Isolation of Plant Mitochondrial RNA from Green Leaves,fied mtRNA is necessary not only for construction of a mitochondrial cDNA library, but also for the analysis of plant mitochondrial transcription. Several methods have been frequently used for isolation of plant mtRNA (.–.). However, these mtRNA preparations may be heavily contaminated by chloroplas作者: 駭人 時(shí)間: 2025-3-23 21:18
Extraction of RNA from Fresh and Frozen Blood,ogemzation as is necessary with solid tissues. However, blood is a particularly problematic tissue from which to isolate RNA because RNA is extremely prone to degradation by ribonucleases, of which red cells are a rich source. Furthermore, blood constituents or their derivatives may inhibit PCR reac作者: 出處 時(shí)間: 2025-3-24 01:57
Isolation of Total RNA from Bacteria,sually occurs complexed with protein from which it must be released. Precautions to be taken against exogenous RNase include the use of plastic gloves, autoclavmg solutions after adding 0.1% (v/v) diethyl pyrocarbonate (DEPC; except Trrs, which reacts), and baking glassware, spatulas, and so forth a作者: ornithology 時(shí)間: 2025-3-24 02:20 作者: agitate 時(shí)間: 2025-3-24 06:57
UV Spectrophotometric Analysis of Ribonucleic Acids,the majority of situations this is carried out using spectrophotometry, which is nondestructive and allows the sample to be recovered for further analysis or manipulation. Spectrophotometry makes use of the fact that there is a relationship between the absorption of ultraviolet light by RNA and its 作者: 星星 時(shí)間: 2025-3-24 13:18 作者: Interlocking 時(shí)間: 2025-3-24 14:54 作者: 急性 時(shí)間: 2025-3-24 20:07
Nonradioactive Northern Blotting,ein,” and “biotin” which are linked through a spacer to a nucleotide (in most cases UTP or dUTP) and are incorporated into specific gene-probes by various methods (e.g., random-priming, nick-translation, or PCR). The detection is based on the specific interaction of the labels with appropriate prote作者: 小溪 時(shí)間: 2025-3-25 01:39 作者: neologism 時(shí)間: 2025-3-25 05:08 作者: Exposition 時(shí)間: 2025-3-25 08:31 作者: BUMP 時(shí)間: 2025-3-25 12:45 作者: MIRE 時(shí)間: 2025-3-25 18:48 作者: constitutional 時(shí)間: 2025-3-25 21:31 作者: exorbitant 時(shí)間: 2025-3-26 01:22 作者: ECG769 時(shí)間: 2025-3-26 08:09
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820174.jpg作者: Epidural-Space 時(shí)間: 2025-3-26 12:10 作者: Humble 時(shí)間: 2025-3-26 16:01
RNA Isolation and Characterization Protocols978-1-59259-570-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Arrhythmia 時(shí)間: 2025-3-26 16:49
Introduction to Isolating RNA,Ribonucleic acid (RNA) is an unbranched polymer of purine (adenine, guanine) and pyrimidine (cytosine, uracil) nucleotides joined by phosphodiester bonds.作者: paltry 時(shí)間: 2025-3-27 00:14
Isolation of Messenger RNA,mRNA comprises approx l–5% of total cellular RNA. Although the actual amount depends on the type of cell and its physiological state, at any given time approx 12,000 genes are being transcribed with approx 500,000 mRNA molecules present in each mammalian cell.作者: Charade 時(shí)間: 2025-3-27 04:28
Isolating RNA with the Cationic Surfactant, Catrimox-14,phase, and the cationic head groups bind to the nucleic acid electrostatically. The precipitate can be dissolved in organic solvents, or it can be redissolved in water by the addition of salt. Selected cationic surfactants are capable of lysing cells, by solubilizmg their protem and lipid components.作者: 分期付款 時(shí)間: 2025-3-27 06:11 作者: 阻撓 時(shí)間: 2025-3-27 12:16 作者: aristocracy 時(shí)間: 2025-3-27 14:42
RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues,many purposes, a simple qualitative analysis of the presence of a specific RNA in tissues may be sufficient, for instance, to establish the persistence in tissues of the genome of an RNA virus. However, when gene expression levels are needed, quantiation of the specific mRNA is an important task.作者: 兇兆 時(shí)間: 2025-3-27 19:42
Wolfgang Uckert,Wolfgang Walther,Ulrike Steine- rienced scientists from universities, research laboratories and industry have the opportunity to discuss their most recent results, and where students and young scientists can learn about the present status of research and applications from some of the most eminent workers in their field. In comm作者: definition 時(shí)間: 2025-3-27 23:22
Christopher E. Dahle,Donald E. Macfarlanee- rienced scientists from universities, research laboratories and industry have the opportunity to discuss their most recent results, and where students and young scientists can learn about the present status of research and applications from some of the most eminent workers in their field. In comm作者: 討厭 時(shí)間: 2025-3-28 03:38
Giorgio Stanta,Serena Bonin,Rosella Perinrtion. This phase is related to the choice of the origin of coordinates and then to the translational symmetry of the system. Hence this sliding motion, or sliding conductivity, is sensitive to the impurity scattering and the Umklapp scattering [8]. These scattering mechanisms result in impurity and作者: muscle-fibers 時(shí)間: 2025-3-28 08:38 作者: SIT 時(shí)間: 2025-3-28 11:43 作者: 業(yè)余愛好者 時(shí)間: 2025-3-28 14:56 作者: syncope 時(shí)間: 2025-3-28 21:00
John Heptinstall magneto- resistance devices, and new‘ magnetic materials needed to be covered. The sections on dielectric properties, ferroelectricity, piezoelectricity, electrostric- tion, and thermoelectric properties have been expanded. Of course, the entire text was critically reviewed, updated, and improved. However, t978-3-642-86538-1作者: 鴕鳥 時(shí)間: 2025-3-29 01:25
Tapas Mukhopadhyay,Jack A. Roth magneto- resistance devices, and new‘ magnetic materials needed to be covered. The sections on dielectric properties, ferroelectricity, piezoelectricity, electrostric- tion, and thermoelectric properties have been expanded. Of course, the entire text was critically reviewed, updated, and improved. However, t978-3-642-86538-1作者: 乳汁 時(shí)間: 2025-3-29 03:52 作者: machination 時(shí)間: 2025-3-29 10:17 作者: 富足女人 時(shí)間: 2025-3-29 12:08 作者: MILK 時(shí)間: 2025-3-29 18:13
Donald E. Macfarlane,Christopher E. Dahlehere students and young scientists can learn about the present status of research and applications from some of the most eminent workers in their field. In comm978-3-642-84867-4978-3-642-84865-0Series ISSN 0171-1873 Series E-ISSN 2197-4179 作者: Between 時(shí)間: 2025-3-29 21:58
David B. Batt,Gordon G. Carmichael,Zhong Liuhere students and young scientists can learn about the present status of research and applications from some of the most eminent workers in their field. In comm978-3-642-84867-4978-3-642-84865-0Series ISSN 0171-1873 Series E-ISSN 2197-4179 作者: 盡管 時(shí)間: 2025-3-30 00:10 作者: 易發(fā)怒 時(shí)間: 2025-3-30 05:50 作者: 跳動(dòng) 時(shí)間: 2025-3-30 09:43
Rainer L?wmorphous ferromagnetics. The fourth edition has been revised and updated with an emphasis on the applications sections, which now cover devices of the next generation of electronics. .978-1-4899-9841-5978-1-4419-8164-6作者: 單獨(dú) 時(shí)間: 2025-3-30 14:25
Dominique Belinmorphous ferromagnetics. The fourth edition has been revised and updated with an emphasis on the applications sections, which now cover devices of the next generation of electronics. .978-1-4899-9841-5978-1-4419-8164-6作者: 閑蕩 時(shí)間: 2025-3-30 19:04
Rai Ajit K. Srivastavamorphous ferromagnetics. The fourth edition has been revised and updated with an emphasis on the applications sections, which now cover devices of the next generation of electronics. .978-1-4899-9841-5978-1-4419-8164-6作者: CANON 時(shí)間: 2025-3-30 23:29 作者: 發(fā)誓放棄 時(shí)間: 2025-3-31 03:10 作者: 合唱團(tuán) 時(shí)間: 2025-3-31 08:42 作者: dragon 時(shí)間: 2025-3-31 10:58 作者: travail 時(shí)間: 2025-3-31 17:12 作者: 后退 時(shí)間: 2025-3-31 21:11
Analysis of RNA by Northern Blotting Using Riboprobes,containing formaldehyde and transferred onto mtrocellulose or nylon membrane. The transferred RNAs on the membrane are then denatured and probed with a labeled .DNA probe or a riboprobe. While a cDNA probe gives the same mformation as the riboprobe, the sensitivity of the detection of mRNA is increa作者: 甜得發(fā)膩 時(shí)間: 2025-4-1 01:03
Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing,ple-to-sample variation in the final yield of the PCR. The efficiency of amplification is also affected by the sequence of the PCR primers, as well as the size and, to some extent, the sequence of the PCR product. For these reasons a prereqursite for an accurate quantitative PCR analysis is that an 作者: floodgate 時(shí)間: 2025-4-1 05:36
Book 1998e means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been devel作者: Solace 時(shí)間: 2025-4-1 07:46
onic properties of graphene heterostructures.Experimentally The last decade has witnessed the discovery of, and dramatic progress in understanding the physics of graphene and related two-dimensional materials. The development of methods for manufacturing and aligning high-quality two-dimensional cry作者: nurture 時(shí)間: 2025-4-1 13:00
Donald E. Macfarlane,Christopher E. DahleTyrol) Austria, was the sixth in a series of meetings to be held at this venue. Four of the earlier meetings were dedicated to issues in the field of conducting polymers, while the winter school held in 1990 was devoted to the new discipline of high-T c superconductivity. This year‘s meeting constit作者: 逃避系列單詞 時(shí)間: 2025-4-1 15:35
Wolfgang Uckert,Wolfgang Walther,Ulrike Steintria, was the sixth in a series of meetings to be held at this venue. Four of the earlier meetings were dedicated to issues in the field of conducting polymers, while the winter school held in 1990 was devoted to the new discipline of high-T c superconductivity. This year‘s meeting constituted a for作者: Arrhythmia 時(shí)間: 2025-4-1 21:00
David B. Batt,Gordon G. Carmichael,Zhong LiuTyrol) Austria, was the sixth in a series of meetings to be held at this venue. Four of the earlier meetings were dedicated to issues in the field of conducting polymers, while the winter school held in 1990 was devoted to the new discipline of high-T c superconductivity. This year‘s meeting constit作者: 貧困 時(shí)間: 2025-4-2 01:56 作者: Cpr951 時(shí)間: 2025-4-2 02:59
Giorgio Stanta,Serena Bonin,Rosella Perinimensional systems, both experimentally and theoretically [1–4]. Above all, systems having a Peierls transition [5] have been investigated in detail. The novelty of this Peierls—Fr?hlich (PF) [6] state lies in the novel possibility of the transport phenomenon being associated with the collective deg作者: reflection 時(shí)間: 2025-4-2 09:57
