標(biāo)題: Titlebook: RNA Detection; Methods and Protocol Imre Gaspar Book 2018 Springer Science+Business Media LLC 2018 Drosophila.ECHO-liveFISH.FISH.RCas9.FISS [打印本頁(yè)] 作者: 毛發(fā) 時(shí)間: 2025-3-21 18:48
書目名稱RNA Detection影響因子(影響力)
書目名稱RNA Detection影響因子(影響力)學(xué)科排名
書目名稱RNA Detection網(wǎng)絡(luò)公開度
書目名稱RNA Detection網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱RNA Detection被引頻次
書目名稱RNA Detection被引頻次學(xué)科排名
書目名稱RNA Detection年度引用
書目名稱RNA Detection年度引用學(xué)科排名
書目名稱RNA Detection讀者反饋
書目名稱RNA Detection讀者反饋學(xué)科排名
作者: CRACY 時(shí)間: 2025-3-21 23:31
Caroline Medioni,Florence Besseities and means for intellectualization of this process concerning such an important information source as the academic texts. In this case the user is faced with the task of finding fragments relevant to the subject of interest, within the vast textual documents often written in a foreign language.作者: ENNUI 時(shí)間: 2025-3-22 01:37 作者: B-cell 時(shí)間: 2025-3-22 05:48 作者: 高射炮 時(shí)間: 2025-3-22 09:51 作者: Neuralgia 時(shí)間: 2025-3-22 12:53
Toshiaki Shigeoka,Jane Jung,Christine E. Holt,Hosung Jungout ICT. As web technology has evolved from Web 1.0, consisting of simple computer systems, to Web 4.0, involving Artificial Intelligence and the Internet of Things, so has e-governance. As a result, there has been a noticeable transformation from a digital government to an algorithmic government. H作者: 精致 時(shí)間: 2025-3-22 17:41 作者: ascetic 時(shí)間: 2025-3-23 00:40 作者: jumble 時(shí)間: 2025-3-23 02:31 作者: Tonometry 時(shí)間: 2025-3-23 06:46 作者: anesthesia 時(shí)間: 2025-3-23 11:45
Joshua S. Titlow,Lu Yang,Richard M. Parton,Ana Palanca,Ilan Davisome one of the top priorities for customs administrations around the world. Customs has a role in safeguarding public values such as safety and security, revenue collection, and stimulation of the economy. Customs administrations are now looking into innovative ways to be able to fulfil their duties作者: 極深 時(shí)間: 2025-3-23 17:07
Catherine Rabouillend recipes for their successful implementation, these initiatives are taking much longer and facing more difficulties than it has been expected. During the outbreak of the Covid-19 pandemic and with the implementation of social distancing, digital transformation has become a major issue. The world h作者: 叫喊 時(shí)間: 2025-3-23 21:07
Yao Xu,Zhi Zhengmework is developed through a qualitative hermeneutic approach involving several different literatures. The framework goes beyond the prevalent focus on substantive needs. It identifies four needs related to the BE process: process-security, relational security, discretion and efficiency and effecti作者: 無(wú)法破譯 時(shí)間: 2025-3-24 01:35
Viravuth P. Yinmany local governments have started smart city initiatives in order to address community issues, improve quality of life, achieve sustainable development, and, overall, make their cities smarter. As a multidimensional concept, smart city contains different components. In addition, to successfully im作者: 外露 時(shí)間: 2025-3-24 03:41 作者: 正論 時(shí)間: 2025-3-24 07:56
Yantao Yang,Mingming Chen,Christopher J. Krueger,Andrew Tsourkas,Antony K. Chennd recipes for their successful implementation, these initiatives are taking much longer and facing more difficulties than it has been expected. During the outbreak of the Covid-19 pandemic and with the implementation of social distancing, digital transformation has become a major issue. The world h作者: Constrain 時(shí)間: 2025-3-24 13:50
Mingming Chen,Yantao Yang,Christopher J. Krueger,Antony K. Chenmmunication Technologies (ICT) may help improve the public sector by contributing to new services and processes that address citizens as well as government-to-government services, involve citizens more directly in decisions being made, and contribute to streamlining work processes and standardizatio作者: CAB 時(shí)間: 2025-3-24 16:17 作者: 維持 時(shí)間: 2025-3-24 23:02
Jasmine Chamiolo,Imre Gaspar,Anne Ephrussi,Oliver Seitzssing Working Group 8.5 (Information Systems in Public Administration), or IFIP WG 8.5, has organized the EGOV series of conferences, which has solidly - tablished itself as one of three core conferences in the research domain of e- Government, e-Governance, and e-Participation. Until last year, EGO作者: 戰(zhàn)役 時(shí)間: 2025-3-25 02:30
Murat Sunbul,Ankita Arora,Andres J?schkebe used to better understand the use of the resources and to suggest improvements. The objective of this paper is to investigate the complementariness of the smart cities and big and Open Data research streams. Two inductive cases concerning different aspects of smart cities, energy and mobility, ar作者: BROTH 時(shí)間: 2025-3-25 04:06
Identifying the m6A Methylome by Affinity Purification and Sequencing,tor. Recent research has uncovered insight into the location and function of m.A sites on a large scale, in part due to the transcriptome-wide identification of m.A sites by high-throughput sequencing (m.A-seq). Here, we present a protocol for m.A-seq, which maps the m.A methylome by affinity purification and sequencing.作者: Humble 時(shí)間: 2025-3-25 08:56
LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled thod utilizes off-the-shelf reagents and direct lysis of the cells without RNA purification, making it a simple and relatively cheap method with high reproducibility and sensitivity compared to previous methods. The advantage with LCM-seq is also that tissue sections are kept intact and thus the positional information of each cell is preserved.作者: CYN 時(shí)間: 2025-3-25 14:30
Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections, and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous . and . mRNAs in . oocytes.作者: preservative 時(shí)間: 2025-3-25 16:04
In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes,netically nonmodified loci. Here, we describe the design, synthesis and injection of nuclease resistant FIT probes into developing . oocytes to detect endogenous localizing mRNAs as wells as to probe function of structural RNA elements.作者: 駁船 時(shí)間: 2025-3-25 22:57
978-1-4939-8418-3Springer Science+Business Media LLC 2018作者: 平 時(shí)間: 2025-3-26 00:27 作者: Modify 時(shí)間: 2025-3-26 05:13 作者: Onerous 時(shí)間: 2025-3-26 10:51
https://doi.org/10.1007/978-1-4939-7213-5Drosophila; ECHO-liveFISH; FISH; RCas9; FISSEQ作者: Flatter 時(shí)間: 2025-3-26 16:11
Imre GasparIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 大吃大喝 時(shí)間: 2025-3-26 18:47
Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas,st can be obtained by performing a series of ISH experiments, followed by a process of image registration and gene expression averaging. Using the overlapping fraction of the genes, concomitantly obtained scRNAseq data can be fitted into the spatial context of the gene expression atlas, complementing the coverage by genes.作者: Heretical 時(shí)間: 2025-3-26 21:42 作者: 乞丐 時(shí)間: 2025-3-27 03:16 作者: 無(wú)所不知 時(shí)間: 2025-3-27 07:17 作者: 腐敗 時(shí)間: 2025-3-27 09:33
Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryafish embryos, an smFISH protocol optimized for zebrafish cryosections, and a user-friendly, automated analysis pipeline for cell segmentation and transcript detection. The software is freely available and can be used to analyze sections of any multicellular organism.作者: 機(jī)制 時(shí)間: 2025-3-27 14:33
In Situ Detection of MicroRNA Expression with RNAscope Probes, provides simultaneous single molecule detection of individual microRNA and its target gene. This method allows for rapid and sensitive detection of noncoding RNA transcripts in frozen tissue sections.作者: 憤慨一下 時(shí)間: 2025-3-27 20:46
Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH, entangled experimental results. Using this method, we observe in living brain tissue spatially constrained nuclear RNA foci under dynamic regulation in response to cellular transcriptional activity with individual cell heterogeneity.作者: 膽大 時(shí)間: 2025-3-27 23:33
Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers,orophores and also dual-color imaging of two distinct RNAs in live bacteria. Our approach uses small RNA tags and small molecule fluorophores for labeling, thereby minimal perturbation on the function and dynamics of the RNA of interest is expected.作者: AMOR 時(shí)間: 2025-3-28 02:40 作者: Neonatal 時(shí)間: 2025-3-28 07:23 作者: wangle 時(shí)間: 2025-3-28 14:24 作者: grounded 時(shí)間: 2025-3-28 14:58 作者: 商業(yè)上 時(shí)間: 2025-3-28 20:17 作者: 有助于 時(shí)間: 2025-3-29 01:28
,Quantification of 2′-,-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Pr recently published accurate and sensitive detection of these modifications on native RNA at a single base resolution by high-throughput sequencing technologies. Relative quantification of these modifications is still under progress and would probably reduce the number of false positives due to 3D R作者: 廚房里面 時(shí)間: 2025-3-29 05:22 作者: lymphedema 時(shí)間: 2025-3-29 07:50 作者: 高腳酒杯 時(shí)間: 2025-3-29 13:10
Axon-TRAP-RiboTag: Affinity Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo, tagged ribosomal protein. We developed axon-TRAP-RiboTag, a TRAP-based method that allows purification and identification of translated mRNAs from distal neuronal axons in mouse, and identified more than 2000 of translated mRNAs in retinal ganglion cell (RGC) axons in vivo. The use of Cre-negative 作者: 鋼筆尖 時(shí)間: 2025-3-29 18:32
LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled thod utilizes off-the-shelf reagents and direct lysis of the cells without RNA purification, making it a simple and relatively cheap method with high reproducibility and sensitivity compared to previous methods. The advantage with LCM-seq is also that tissue sections are kept intact and thus the pos作者: GNAW 時(shí)間: 2025-3-29 23:22 作者: 線 時(shí)間: 2025-3-30 00:22
Single mRNA Molecule Detection in ,,s increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolu作者: Encumber 時(shí)間: 2025-3-30 05:40
Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embrygmentation, transcripts can be assigned to different cellular compartments resulting in quantitative information on transcript levels at subcellular resolution. The use of smFISH in zebrafish has been limited by the lack of protocols and an automated image analysis pipeline for samples of multicellu作者: 蜿蜒而流 時(shí)間: 2025-3-30 11:11 作者: BLUSH 時(shí)間: 2025-3-30 12:57 作者: 可憎 時(shí)間: 2025-3-30 18:12 作者: Debrief 時(shí)間: 2025-3-30 21:13 作者: 刻苦讀書 時(shí)間: 2025-3-31 03:58 作者: concubine 時(shí)間: 2025-3-31 08:11 作者: 懸掛 時(shí)間: 2025-3-31 11:04 作者: 礦石 時(shí)間: 2025-3-31 14:38
Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH,iving cells. Using chemically engineered linear oligonucleotide probes that emit fluorescence upon hybridization to target RNA, we have devised an imaging method suitable for studies of the dynamic regulation of nuclear RNPs, an important and yet poorly understood cellular pathway of gene expression作者: placebo 時(shí)間: 2025-3-31 18:09 作者: 幻影 時(shí)間: 2025-3-31 22:26
Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers, Here, we describe a novel live-cell RNA imaging method using fluorophore- and quencher-binding aptamers, which can be genetically fused to the ROI. In this method, new membrane permeable and nonfluorescent fluorophore–quencher conjugates were utilized, and we showed that their fluorescence increase作者: lavish 時(shí)間: 2025-4-1 04:24 作者: diskitis 時(shí)間: 2025-4-1 06:09 作者: 盤旋 時(shí)間: 2025-4-1 12:19
Lilia Ayadi,Yuri Motorin,Virginie Marchandroblog account type prediction framework “POP-MAP”. To study the problem at a cross-language level, we harvested and learned from a multi-lingual Twitter dataset, which allows us to achieve better classification performance, as compared to various state-of-the-art baselines.
