作者: 剝削 時間: 2025-3-22 00:16 作者: 精美食品 時間: 2025-3-22 02:39 作者: Ringworm 時間: 2025-3-22 05:58
CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in ,g. The class 2 Type VI CRISPR-Cas13 system is an RNA-guided RNA-nuclease system capable of binding and cleaving target single-stranded RNA substrates in a sequence-specific manner. In addition to RNA interference, the Cas13a system has application from manipulating RNA modifications, to editing RNA 作者: lattice 時間: 2025-3-22 11:31 作者: 為敵 時間: 2025-3-22 15:38
Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning,s. Widely used in animal research, still few studies on plants have been carried out. We have applied this technique to the plant–nematode interaction by isolating feeding cells (giant cells; GCs) immersed inside complex swelled root structures (galls) induced by root-knot nematodes. For this purpos作者: Fibrinogen 時間: 2025-3-22 19:47 作者: 有法律效應(yīng) 時間: 2025-3-22 21:38
Quantitative Analysis of Plant miRNA Primary Transcripts, controlled since both excess and deficiency of a given microRNA may be deleterious to plant cell. MicroRNA expression regulation can occur at several stages of their biogenesis pathway. One of the most important of these regulatory checkpoints is transcription efficiency. mirEX database is a tool f作者: Maximizer 時間: 2025-3-23 03:04 作者: 修剪過的樹籬 時間: 2025-3-23 09:17
,Analysis of RBP Regulation and Co-regulation of mRNA 3′ UTR Regions in a Luciferase Reporter Systemelements can be fused to the luciferase gene, and as a result effect protein output by changing rates of transcription, rates of translation, or mRNA stability. This protocol focuses on probing the function of RNA-binding proteins (RBPs) through their interactions with the 3′ untranslated region (UT作者: 轉(zhuǎn)換 時間: 2025-3-23 10:03 作者: SHOCK 時間: 2025-3-23 14:19 作者: 清楚 時間: 2025-3-23 21:40
Purification and Analysis of Chloroplast RNAs in ,,phere. Additionally, chloroplasts regulate energy production, metabolite synthesis, and stress responses in plants and algae. Chloroplasts possess a notably complex RNA metabolism that includes RNA processing, editing, splicing, and regulation by various RNA-binding proteins. Highly purified chlorop作者: 取消 時間: 2025-3-24 01:10
In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes, patterns. Therefore, in situ detection of mature miRNAs is important for understanding the functions for both miRNAs and their targets. The construction of promoter-reporter fusions and examination of their in planta expression has been widely used and the results obtained thus far are rather infor作者: Friction 時間: 2025-3-24 05:55
Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs,ence of a transcript but also indicates size and relative comparison of transcript abundance on a single membrane. In recent years it has been aptly adapted to validate and study the size and expression of small noncoding RNAs. Here, we describe protocols employed in our laboratory for conventional 作者: Lucubrate 時間: 2025-3-24 08:18 作者: 暗語 時間: 2025-3-24 12:51
Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from , and , and are implicated in significant diseases and metabolic disorders. Elucidation of biogenesis mechanisms and functional characterization of sRNAs is often achieved using tools such as separation of small-sized RNA and deep sequencing. Although RNA interference pathways, such as quelling and meiotic si作者: CUMB 時間: 2025-3-24 14:50 作者: Pulmonary-Veins 時間: 2025-3-24 22:03 作者: cortex 時間: 2025-3-25 02:20
Book 2021Latest editionntional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational 作者: 加劇 時間: 2025-3-25 06:24
CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in ,iviral defense. We cover all the necessary information for cloning the Cas13 protein, crRNA guide cassette, performing transient .-mediated expression of the necessary Cas13a components and target RNA-virus, visualization of virus infection, and molecular quantification of viral accumulation using quantitative PCR.作者: cataract 時間: 2025-3-25 09:12
Strand-Specific RNA-Seq Applied to Malaria Samples,this method, we prepared high-quality strand-specific RNA-seq libraries from RNA extracted from the human malaria parasite . The libraries are compatible with Illumina.’s sequencers Genome Analyzer and Hi-Seq. The method can however be easily adapted to other platforms.作者: LATER 時間: 2025-3-25 12:15 作者: Sinus-Node 時間: 2025-3-25 18:36
Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses,its use. This chapter provides a simple, step-by-step dissection protocol for the rapid isolation of aphid bacteriocytes. We then describe an adapted protocol for efficient extraction and purification of bacteriocyte RNA that can be used for most downstream transcriptomic analyses.作者: 線 時間: 2025-3-25 22:40
Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequentiahod). The isolated fungal cells are free of contaminants from the host plants, and remain viable, providing high-quality RNA for library construction. This method can be modified to isolate the infection structures of many other plant pathogens from plant tissue.作者: Colonnade 時間: 2025-3-26 00:28
Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning,e, a protocol that combines good morphology preservation with RNA integrity maintenance was developed, and successfully applied to Arabidopsis and tomato galls. Specifically, early developing GCs at 3 and 7?days post-infection (dpi) were analyzed; RNA from LCM GCs was amplified and used successfully for microarray assays.作者: Soliloquy 時間: 2025-3-26 06:04
,Analysis of RBP Regulation and Co-regulation of mRNA 3′ UTR Regions in a Luciferase Reporter SystemR), thus examining gene expression regulation on the mRNA level. Assessment of 3′ UTR sequence requirements, as well as single and co-regulatory roles of RBPs in regulation of mRNAs will be discussed.作者: Decibel 時間: 2025-3-26 11:45 作者: 兇猛 時間: 2025-3-26 16:17 作者: Enzyme 時間: 2025-3-26 17:51 作者: 戰(zhàn)役 時間: 2025-3-26 22:52
Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs,northern analysis with total RNA/mRNA to study gene expression and validation of small noncoding RNAs using low molecular weight fraction of RNAs. A brief account on the recent advancements for improving the sensitivity and efficiency of northern blot detection is also included in this chapter.作者: 小官 時間: 2025-3-27 02:29
978-1-0716-0745-9Springer Science+Business Media, LLC, part of Springer Nature 2021作者: 放棄 時間: 2025-3-27 08:52
RNA Abundance Analysis978-1-0716-0743-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 慷慨援助 時間: 2025-3-27 13:03 作者: 江湖騙子 時間: 2025-3-27 16:24
https://doi.org/10.1007/978-1-0716-0743-5mRNAs; Small RNAs; Gene expression; RNA expression; Next-generation sequencing; RNA-protein interactions作者: Cursory 時間: 2025-3-27 18:53 作者: 濃縮 時間: 2025-3-28 01:42 作者: 懸掛 時間: 2025-3-28 06:01 作者: Insubordinate 時間: 2025-3-28 07:01 作者: Virtues 時間: 2025-3-28 11:02
Xueqing Maggie Lu,Karine Le Rochelang, zahlreiche namhafte Autoren aus Wissenschaft und Praxis zu gewinnen, deren Erkenntnisse in der ersten Auflage, die Anfang 1999 erschienen ist, zusammenzuführen. Der Erfolg des Buches best?tigte seine K978-3-409-38990-7978-3-322-93181-8作者: 不能和解 時間: 2025-3-28 17:13
1064-3745 ion. provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies..978-1-0716-0745-9978-1-0716-0743-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: nominal 時間: 2025-3-28 20:49 作者: 公式 時間: 2025-3-29 02:41
Ankur R. Bhardwaj,Ritu Pandey,Manu Agarwal,Surekha Katiyar-Agarwalram, and the trend toward conducting EC with mobile devices. ?Other major developments are the expansion of EC globally, especially in China where you can find the world‘s largest EC company. ?Much at978-3-319-86460-0978-3-319-58715-8Series ISSN 2192-4333 Series E-ISSN 2192-4341 作者: Increment 時間: 2025-3-29 06:52 作者: 血友病 時間: 2025-3-29 09:59
Book 2021Latest editiontips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .RNA Abundance Analysis: Methods and Protocols, Second Edition. provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies..作者: 忙碌 時間: 2025-3-29 12:31
rn und Dienstleistungen. Nach . (1997, S. 71) geht es beim Electronic Commerce grunds?tzlich um ?Transaktionen auf elektronischen Marktpl?tzen“. Diese umfassende Beschreibung l??t sich genauer fassen. Nach unserer Meinung beschreibt Electronic Commerce die . und . unterschiedlicher . und . auf der G作者: 英寸 時間: 2025-3-29 19:30 作者: 縮短 時間: 2025-3-29 20:55 作者: 諷刺 時間: 2025-3-30 03:10 作者: 廚房里面 時間: 2025-3-30 05:36 作者: Metastasis 時間: 2025-3-30 10:17 作者: Fibrinogen 時間: 2025-3-30 13:04 作者: 疾馳 時間: 2025-3-30 18:05 作者: 鞭子 時間: 2025-3-30 21:32 作者: 暫時過來 時間: 2025-3-31 04:30
