標(biāo)題: Titlebook: R-Loops; Methods and Protocol Andrés Aguilera,Alexey Ruzov Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen [打印本頁] 作者: 乳缽 時(shí)間: 2025-3-21 18:24
書目名稱R-Loops影響因子(影響力)
書目名稱R-Loops影響因子(影響力)學(xué)科排名
書目名稱R-Loops網(wǎng)絡(luò)公開度
書目名稱R-Loops網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱R-Loops被引頻次
書目名稱R-Loops被引頻次學(xué)科排名
書目名稱R-Loops年度引用
書目名稱R-Loops年度引用學(xué)科排名
書目名稱R-Loops讀者反饋
書目名稱R-Loops讀者反饋學(xué)科排名
作者: 生意行為 時(shí)間: 2025-3-21 21:54
https://doi.org/10.1007/978-1-0716-2477-7DNA-RNA hybrids; Genomic stress and instability; Genome dynamics; Electron microscopy; Topology; Genome-w作者: VEST 時(shí)間: 2025-3-22 02:50
978-1-0716-2479-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: SPASM 時(shí)間: 2025-3-22 08:05 作者: defile 時(shí)間: 2025-3-22 10:53 作者: 健忘癥 時(shí)間: 2025-3-22 14:50
Highly Purified Top1-Bound DNA Fragments,in, CPT) in living cells. Various methods are available to detect Top1ccs in cultured cells, including protocols based on the use of specific antibodies. Here, we describe a protocol to isolate Top1ccs at high purity, which does not depend on antibodies.作者: 松緊帶 時(shí)間: 2025-3-22 19:42
Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids,m.C) marked RNA:DNA hybrids containing the m.C modified RNA strand add another layer of regulation to the R-loop structure. Here we describe the detection of m.C modifications in the context of RNA:DNA hybrids using immunostaining, and identification of specific binding partners of m.C marked RNA:DNA hybrids using pull-down method.作者: 酷熱 時(shí)間: 2025-3-22 23:07 作者: 清洗 時(shí)間: 2025-3-23 01:55 作者: 發(fā)源 時(shí)間: 2025-3-23 08:54
Henriette Stoy,Joel Luethi,Fabienne K. Roessler,Johannes Riemann,Andres Kaech,Massimo Lopesf similarity, e.g. in a closed hemispherical container, also led to serious difficulties. Consequently, the cause of difficulties should be sought in the physical statement of the problem, which probably does not take into account an essential mechanism limiting the growth of velocities in the flow 作者: GROWL 時(shí)間: 2025-3-23 10:49
Konstantina Skourti-Stathakiion of an electric current passing through the fluid with its own magnetic field. It cannot be said that this direction was created ex nihilo: individual studies related to the flows driven in a current-carrying medium in the absence of external magnetic fields appeared in the sixties; in the thirti作者: 冥界三河 時(shí)間: 2025-3-23 17:33 作者: 者變 時(shí)間: 2025-3-23 20:50 作者: Ganglion 時(shí)間: 2025-3-23 22:38
Carolin B. Wagner,Brian Lukeecisively higher than the one of the reserved capacity. This issue leads to a two-fold necessity: implementing a better know-how system in order to better contain the customers load fluctuation and the necessity of distributed generation implementation, in order to help load shedding. The third issu作者: 咒語 時(shí)間: 2025-3-24 05:08
Galina Glousker,Rita Valador Fernandes,Marianna Feretzaki,Joachim Lingnerys which decarbonisation, decentralisation and digitalisation trends in the electricity sector will eventually shape future of distribution grids. It will be relevant to students, researchers, and policymakers interested in energy economics, the political economy of energy, and the electricity sector..978-3-030-98069-6作者: Chronological 時(shí)間: 2025-3-24 08:47 作者: Repatriate 時(shí)間: 2025-3-24 12:25
Jessica Marinello,Giovanni Capranicot, government action on policies, etc. Then, the multi-agent system can simulate dynamic operation of the national economy. We are able to obtain a weekly input–output table through the ARE economic model, since our ARE model could be simulated on a weekly basis. In addition, this chapter also discu作者: BROTH 時(shí)間: 2025-3-24 17:26
émilie Vlachos-Breton,Marc Drolet book. .The book can serve as a compact textbook for lectures, as an introduction for hardware system and electric control systems, and mechanical systems. Chapters for electromagnetism or ones for electric circuits are suitable for a lecture over a semester..978-3-031-44004-5978-3-031-44002-1作者: 驕傲 時(shí)間: 2025-3-24 21:56 作者: 喪失 時(shí)間: 2025-3-25 01:43
Adele Alagia,Ruth F. Ketley,Monika Gullerova electricity deregulation, this book is a must-read for academics, students and researchers with an interest in electric power industry restructuring. It also has direct relevance for engineers, regulators and other decision makers in companies and governmental agencies concerned with energy issues.978-1-4899-8731-0978-1-4419-5612-5作者: BOOM 時(shí)間: 2025-3-25 04:38 作者: 前面 時(shí)間: 2025-3-25 08:04
1064-3745 hooting and avoiding known pitfalls.?.Authoritative and practical, .R-Loops: Methods and Protocols. serves as an ideal resource for those working on R-loop ho978-1-0716-2479-1978-1-0716-2477-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: GLARE 時(shí)間: 2025-3-25 14:25
Direct R-Loop Visualization on Genomic DNA by Native Automated Electron Microscopy,e preparation procedures that protect R-loop stability allows for direct visualization of R-loop structures on genomic DNA, independently from probes. Combining single-molecule information and DNA content assessment, this approach provides direct estimations of R-loop frequency, size, and burden on 作者: FANG 時(shí)間: 2025-3-25 16:47
Detection of R-Loop Structures by Immunofluorescence Using the S9.6 Monoclonal Antibody,me-wide, whereas Immunofluorescence is performed to visualize R-loops in single cells. While mapping R-loops genome-wide is very important for identifying and studying their location-specific role, microscopy offers the advantage of spatial information and the ability to quantify them on a single ce作者: ATP861 時(shí)間: 2025-3-26 00:03 作者: Excise 時(shí)間: 2025-3-26 03:21 作者: Judicious 時(shí)間: 2025-3-26 04:32
Sónia Silva,Cristina Guillén-Mendoza,Andrés Aguilera作者: 誘拐 時(shí)間: 2025-3-26 10:06 作者: FLASK 時(shí)間: 2025-3-26 13:56
1064-3745 expertsThis detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues wi作者: Junction 時(shí)間: 2025-3-26 17:04
Direct R-Loop Visualization on Genomic DNA by Native Automated Electron Microscopy,abundance and/or size has been suggested to interfere with the DNA replication process, contributing to genome instability. Most available approaches to monitor R-loops are based on antibodies/enzymes that cannot effectively distinguish R-loops from DNA–RNA hybrids and assess R-loop size and frequen作者: 形容詞詞尾 時(shí)間: 2025-3-26 22:02 作者: Sinus-Rhythm 時(shí)間: 2025-3-27 04:17 作者: BILK 時(shí)間: 2025-3-27 06:07 作者: 鉤針織物 時(shí)間: 2025-3-27 10:50 作者: Plaque 時(shí)間: 2025-3-27 17:00 作者: 違抗 時(shí)間: 2025-3-27 18:31 作者: 異端 時(shí)間: 2025-3-27 22:45 作者: caldron 時(shí)間: 2025-3-28 04:51
,Detection and Quantification of RNA Modifications on RNA–DNA Hybrids Using SID-UPLC-MS/MS,urce of genomic instability and are involved in regulation of several important biological processes in eukaryotic cells. A growing body of experimental evidence suggests that RNA moieties of RNA–DNA hybrids may convey RNA modifications influencing various aspects of R-loop biology. Here we present 作者: 王得到 時(shí)間: 2025-3-28 06:53 作者: milligram 時(shí)間: 2025-3-28 13:22 作者: Comedienne 時(shí)間: 2025-3-28 14:48
R-Loops and Mitochondrial DNA Metabolism,transcription and other aspects of DNA metabolism. The study of R-loops in mitochondria is in its infancy, and yet there is already clear evidence that they are predominantly located in the major regulatory region of the mammalian?mitochondrial genome. Here, we describe how mitochondrial R-loops hav作者: Condense 時(shí)間: 2025-3-28 19:54 作者: 腐蝕 時(shí)間: 2025-3-29 00:29
R-Loop Immunoprecipitation: A Method to Detect R-Loop Interacting Factors,nt biological roles. However, perturbation of R-loop levels represents a source of DNA damage and genome instability resulting in human diseases, including cancer and neurodegeneration. In this chapter, we describe a protocol which allows detection of R-loop interactors using affinity purification w作者: 與野獸博斗者 時(shí)間: 2025-3-29 06:45
,Studies on Protein–RNA:DNA Hybrid Interactions by Microscale Thermophoresis (MST),. MST uses localized temperature fields to measure the diffusion rates of the free and bound states of a fluorescently labeled protein, and to determine the dissociation constant . by fitting of the binding isotherm with a 1:1 binding model. Here, we describe the use of MST for quantitative analysis作者: GROG 時(shí)間: 2025-3-29 09:08 作者: BILK 時(shí)間: 2025-3-29 12:23
Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids,m.C) marked RNA:DNA hybrids containing the m.C modified RNA strand add another layer of regulation to the R-loop structure. Here we describe the detection of m.C modifications in the context of RNA:DNA hybrids using immunostaining, and identification of specific binding partners of m.C marked RNA:DN作者: 抗體 時(shí)間: 2025-3-29 17:49
In Vitro Binding of GADD45A to RNA:DNA Hybrids,nstability, cells also harness RNA–DNA hybrids in scheduled, “regulatory,” R-loops to control gene expression. One regulatory role involves epigenetic gene regulation by the R-loop reader Growth Arrest and DNA Damage 45A (GADD45A). This small stress related protein promotes DNA demethylation by recr作者: 停止償付 時(shí)間: 2025-3-29 22:34 作者: 共棲 時(shí)間: 2025-3-30 02:02 作者: Surgeon 時(shí)間: 2025-3-30 06:10
,Detection and Quantification of RNA Modifications on RNA–DNA Hybrids Using SID-UPLC-MS/MS,a protocol for quantitative analysis of RNA modifications on RNA–DNA hybrids using stable-isotope dilution ultraperformance liquid chromatography coupled with tandem mass spectrometry (SID-UPLC-MS/MS). Supplemented by other techniques, this method can be instrumental in deciphering the roles of RNA modifications in R-loop metabolism.作者: 勛章 時(shí)間: 2025-3-30 08:25 作者: 使腐爛 時(shí)間: 2025-3-30 13:27
,Studies on Protein–RNA:DNA Hybrid Interactions by Microscale Thermophoresis (MST), of the interaction of the N-terminal his-tagged 6-methyladenine (m.A) reader protein YTHDF2 with m.A modified and unmodified RNA, in single-strand configuration or with RNA:DNA hybrid substrates. The described protocol is also suitable for studies of interactions with proteins binding to double-stranded RNA or DNA substrates.作者: 江湖騙子 時(shí)間: 2025-3-30 17:23 作者: Colonoscopy 時(shí)間: 2025-3-30 22:24
Book 2022allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids .in vitro. and .in vivo.. Several protocols permit studying non-canonical RNA n作者: 拋棄的貨物 時(shí)間: 2025-3-31 03:46
Detection of R-Loops by In Vivo and In Vitro Cytosine Deamination in , genome integrity. Here, we describe the protocols used in the yeast . to infer the presence of?R-loops through increased AID-induced DNA damage, measured as increased recombination or Rad52 foci formation as well as to detect single R-loop molecules and determine their length at particular genomic sites via bisulfite treatment and amplification.作者: Pedagogy 時(shí)間: 2025-3-31 06:11 作者: macular-edema 時(shí)間: 2025-3-31 09:36 作者: 寬度 時(shí)間: 2025-3-31 16:00 作者: 敲詐 時(shí)間: 2025-3-31 18:46
,RNase H1 Hybrid-Binding Domain-Based Tools for Cellular Biology Studies of DNA–RNA Hybrids in Mammanowledge about R loops and factors associated with their formation and removal. Here, we describe the use of fluorescently tagged HBD, the hybrid-binding domain of RNase H1, as a tool for analyzing DNA–RNA hybrids in different contexts using live-cell microscopy and immunofluorescence experiments.作者: 不吉祥的女人 時(shí)間: 2025-3-31 22:37
,DNA–RNA Hybrids at Telomeres in Budding Yeast,ect recovered material, and furthermore allows the precipitation of other proteins from the identical cross-linked material. Although both methods are successful in terms of detecting DNA–RNA hybrids at telomeres, the R-ChIP method yields an approximately ten-fold increased enrichment.作者: 誰在削木頭 時(shí)間: 2025-4-1 03:49
R-Loops and Mitochondrial DNA Metabolism, oft-overlooked issues of RNA–DNA hybrids . being synonymous with R-loops, and adventitious RNA hybridization to DNA, are tackled head on; and possible new approaches are described and placed in the context of future research lines that could reveal the detailed roles of R-loops in the metabolism of mitochondrial DNA.作者: 高射炮 時(shí)間: 2025-4-1 07:20
R-Loop Immunoprecipitation: A Method to Detect R-Loop Interacting Factors,f synthetic competitors and RNase H treatment are described to verify the specificity of identified R-loop-binding factors. The identification of new R-loop interacting factors and the characterization of their involvement in R-loop biology provides a powerful resource to study the role of these important structures in health and disease.作者: 女歌星 時(shí)間: 2025-4-1 11:48
model equations are explained in detail, including a survey on boundary conditions, recombination rate models, carrier and current density expressions. The focus of this thesis is on the modeling of charge transport in quantum optical devices, that are typically operated at extremely low temperatur
