標題: Titlebook: Quantitative Real-Time PCR; Methods and Protocol Roberto Biassoni,Alessandro Raso Book 2014 Springer Science+Business Media New York 2014 D [打印本頁] 作者: Hazardous 時間: 2025-3-21 16:37
書目名稱Quantitative Real-Time PCR影響因子(影響力)
書目名稱Quantitative Real-Time PCR影響因子(影響力)學科排名
書目名稱Quantitative Real-Time PCR網(wǎng)絡公開度
書目名稱Quantitative Real-Time PCR網(wǎng)絡公開度學科排名
書目名稱Quantitative Real-Time PCR被引頻次
書目名稱Quantitative Real-Time PCR被引頻次學科排名
書目名稱Quantitative Real-Time PCR年度引用
書目名稱Quantitative Real-Time PCR年度引用學科排名
書目名稱Quantitative Real-Time PCR讀者反饋
書目名稱Quantitative Real-Time PCR讀者反饋學科排名
作者: CAMEO 時間: 2025-3-21 21:49
Selection of Reliable Reference Genes for RT-qPCR Analysis,nce gene quality in subsequent studies. For screening studies including a large, unbiased set of genes (e.g., complete miRNome) an alternative normalization method can be used: global mean normalization. This chapter also describes how the data from such studies can be used to identify reference gen作者: 運氣 時間: 2025-3-22 00:45 作者: 貞潔 時間: 2025-3-22 06:39
Clinical Applications Using Digital PCR,ib. These agents are effective at reducing out-of-control cell cycling and tumor proliferation, but only if downstream signaling kinases and phosphatases are not mutated. Known oncogenes such as BRAF V600E and KRAS G12/13 that are constitutively activated render these treatments ineffective. The use作者: antecedence 時間: 2025-3-22 10:57
Book 2014y materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and authoritative, .Quantitative Real-Time PCR: Methods and Protocols. aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of inv作者: 莎草 時間: 2025-3-22 13:54
Gemma Johnson,Afif Abdel Nour,Tania Nolan,Jim Huggett,Stephen Bustin作者: 輕信 時間: 2025-3-22 18:44
Benedikt Kirchner,Vijay Paul,Irmgard Riedmaier,Michael W. Pfaffl作者: 巫婆 時間: 2025-3-22 22:49
Simon Wadle,Stefanie Rubenwolf,Michael Lehnert,Bernd Faltin,Manfred Weidmann,Frank Hufert,Roland Zen作者: 易受騙 時間: 2025-3-23 03:33
Eddi Di Marco in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec978-3-030-36235-5978-3-030-36233-1Series ISSN 0166-6991 Series E-ISSN 2542-8292 作者: 敲詐 時間: 2025-3-23 09:23 作者: optional 時間: 2025-3-23 11:49 作者: 凌辱 時間: 2025-3-23 16:39
Michael Eikmans,Jacqueline D. H. Anholts,Frans H. J. Claas in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec978-3-030-36235-5978-3-030-36233-1Series ISSN 0166-6991 Series E-ISSN 2542-8292 作者: 撤退 時間: 2025-3-23 18:11 作者: 賠償 時間: 2025-3-23 23:55
Francisco Bizouarn in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec978-3-030-36235-5978-3-030-36233-1Series ISSN 0166-6991 Series E-ISSN 2542-8292 作者: arterioles 時間: 2025-3-24 06:09
in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec978-3-030-36235-5978-3-030-36233-1Series ISSN 0166-6991 Series E-ISSN 2542-8292 作者: pineal-gland 時間: 2025-3-24 07:32 作者: athlete’s-foot 時間: 2025-3-24 13:44
e older Hotelling-inspired theory of cake-eating and the economics of search and R&D designed to uncover new and cheaper sources of supply. It provides an entree to the whole subject of resource economics, as well as many new discoveries which will be of interest to experienced researchers.作者: FLING 時間: 2025-3-24 17:27
Domenico Russo,Mauro Severo Malnatiility, and so the author sets forth a logical variant of the massive modularity hypothesis in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec作者: expository 時間: 2025-3-24 19:50
Francesco Broccoloility, and so the author sets forth a logical variant of the massive modularity hypothesis in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec作者: Medley 時間: 2025-3-25 02:37 作者: 巨頭 時間: 2025-3-25 06:30
Samantha Mascelliility, and so the author sets forth a logical variant of the massive modularity hypothesis in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec作者: 收養(yǎng) 時間: 2025-3-25 11:32
Angela N. Barrett,Lyn S. Chittyility, and so the author sets forth a logical variant of the massive modularity hypothesis in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec作者: GUILT 時間: 2025-3-25 12:57 作者: 拔出 時間: 2025-3-25 19:10 作者: ABIDE 時間: 2025-3-25 21:40 作者: obtuse 時間: 2025-3-26 03:29 作者: Anticonvulsants 時間: 2025-3-26 06:48 作者: archenemy 時間: 2025-3-26 08:29
Developing Noninvasive Diagnosis for Single-Gene Disorders: The Role of Digital PCR,small qPCR reactions, some of which contain the target molecule and some which do not; the proportion of positive reactions can be used to calculate the concentration of targets in the initial sample. Here we discuss the use of digital PCR as an accurate approach to NIPD for single-gene disorders.作者: Mystic 時間: 2025-3-26 13:04
Real-Time PCR Detection of , in the Diagnosis of Community-Acquired Pneumonia,ime PCR..Here we describe the Scorpion-probe real-time PCR-based assay that offers thermodynamic advantages due to its kinetic reaction and provides faster performances compared to a classical double-labeled probe-based assays.作者: JAUNT 時間: 2025-3-26 19:19
Mediator Probe PCR: Detection of Real-Time PCR by Label-Free Probes and a Universal Fluorogenic Repequence tag, the mediator. Further protocols for the setup of reactions, to detect either DNA or RNA targets with clinical diagnostic target detection as models, are explained. Ready to use designs for URs are suggested and guidelines for their de novo design are provided as well, including a protocol for UR signal generation characterization.作者: chiropractor 時間: 2025-3-26 21:44
Posttranscriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA e real mRNA and miRNA transcriptomic data as well as in silico target predictions. This chapter addresses the workflow and methods one can apply for expression profiling and the integrative analysis of mRNA and miRNA data, as well as how to analyze and interpret results, and how to build up models of posttranscriptional regulatory networks.作者: HERTZ 時間: 2025-3-27 03:37
Book 2014bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications作者: strdulate 時間: 2025-3-27 08:26
Twenty Years of qPCR: A Mature Technology?,ts use has been grown exponentially. More recent technological advancements have extended the field of applications ranging from high-resolution melting detection to digital PCR. Nowadays, it is a very accessible technique, but some pitfalls should be overcome in order to achieve robust and reliable作者: RAGE 時間: 2025-3-27 10:06 作者: Bumble 時間: 2025-3-27 15:46 作者: 不可救藥 時間: 2025-3-27 18:23
Introduction to Digital PCR,a routine molecular biology assay. This exciting new technique provides quantitative and detection capabilities that by far surpass other methods currently used. This chapter is an overview of some of the applications currently being performed using dPCR as well as the fundamental concepts and techn作者: 膽小鬼 時間: 2025-3-28 01:19
mRNA and microRNA Purity and Integrity: The Key to Success in Expression Profiling,s, using micro-array, RT-qPCR (Reverse-Transcription quantitative PCR), or Next-Generation-Sequencing by RNA-Seq or small-RNA Seq. Therefore, assessment of RNA integrity and purity is very essential prior to gene expression analysis of sample RNA to ensure the accuracy of any downstream applications作者: concubine 時間: 2025-3-28 04:39 作者: vocation 時間: 2025-3-28 08:51 作者: 指派 時間: 2025-3-28 12:23
A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detect of neoplastic progression. HR-HPV detection and genotyping is the most effective and accurate approach in screening of the early cervical lesions and cervical cancer, although also the HR-HPV DNA load is considered an ancillary marker for persistent HPV infection..Here, it is described an in-house 作者: Handedness 時間: 2025-3-28 16:13
Real-Time PCR Detection of , in the Diagnosis of Community-Acquired Pneumonia,ic acid can be amplified by PCR upon clinical sample extraction using specific primers for classical qualitative PCR and primers and probes for real-time PCR..Here we describe the Scorpion-probe real-time PCR-based assay that offers thermodynamic advantages due to its kinetic reaction and provides f作者: 觀點 時間: 2025-3-28 20:09
A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition,ue cultures is a major problem because it can determine various and severe alterations in cellular function..Thus methods able to detect and trace cell cultures with Mollicutes contamination are needed in the monitoring of cells grown under good manufacturing practice conditions, and cell lines in c作者: 滔滔不絕的人 時間: 2025-3-29 01:07 作者: Inoperable 時間: 2025-3-29 03:19
A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR,umor. Up-to-date conclusive diagnosis is generally achieved through fluorescence in situ hybridization or karyotyping analysis..Herein, we report an alternative, reliable assay for rapidly defining vertical cancer transmission to the fetus by using quantitative polymerase chain reaction. Our assay i作者: 圓錐體 時間: 2025-3-29 08:18 作者: LIMN 時間: 2025-3-29 11:58
Posttranscriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA miRNA) regulatory interactions facilitate the understanding of fine-tuning of gene expression and might allow better estimation of protein synthesis. However, in silico predictions of mRNA–microRNA interactions do not take into account the specific transcriptomic status of the biological system and 作者: connoisseur 時間: 2025-3-29 16:41
Clinical Applications Using Digital PCR, (Strain and Richman, Curr Opin HIV AIDS 8:106–110, 2013) and becoming prevalent in the nonscientific literature. Instead of generically using a “one treatment fits all” approach that may have varying levels of effectiveness to different patients, patient-specific molecular profiling based on the ge作者: 加入 時間: 2025-3-29 21:42
Developing Noninvasive Diagnosis for Single-Gene Disorders: The Role of Digital PCR, material for noninvasive prenatal diagnosis (NIPD) from early pregnancy. The relatively high levels of maternal background can make detection of paternally inherited point mutations challenging. Diagnosis of inheritance of autosomal recessive disorders using qPCR is even more challenging due to the作者: esoteric 時間: 2025-3-30 03:05
1064-3745 notes and implementation advice from the experts.Includes su.Quantitative Real-Time PCR: Methods and Protocols. focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which r作者: Detonate 時間: 2025-3-30 07:57 作者: coagulate 時間: 2025-3-30 09:21 作者: 使服水土 時間: 2025-3-30 12:46
Methods in Molecular Biologyhttp://image.papertrans.cn/q/image/780966.jpg作者: electrolyte 時間: 2025-3-30 19:31 作者: 曲解 時間: 2025-3-31 00:20
Circulating Cell-Free DNA in Cancer,This papers deals with the preanalytical and analytical phase of cell-free DNA analysis, highlighting some criticism on sample collection and extraction. We describe a method to accurately quantify total cfDNA in plasma and our particular approach to the measurement of tumor deriving cfDNA.作者: 600 時間: 2025-3-31 04:22 作者: 易受騙 時間: 2025-3-31 05:01 作者: Creditee 時間: 2025-3-31 10:07
Introduction to Digital PCR,a routine molecular biology assay. This exciting new technique provides quantitative and detection capabilities that by far surpass other methods currently used. This chapter is an overview of some of the applications currently being performed using dPCR as well as the fundamental concepts and techniques this technology is based on.作者: Fallibility 時間: 2025-3-31 16:52
