標(biāo)題: Titlebook: Quantitative Methods in Proteomics; Katrin Marcus,Martin Eisenacher,Barbara Sitek Book 2021Latest edition Springer Science+Business Media, [打印本頁] 作者: GERM 時間: 2025-3-21 19:19
書目名稱Quantitative Methods in Proteomics影響因子(影響力)
書目名稱Quantitative Methods in Proteomics影響因子(影響力)學(xué)科排名
書目名稱Quantitative Methods in Proteomics網(wǎng)絡(luò)公開度
書目名稱Quantitative Methods in Proteomics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Quantitative Methods in Proteomics被引頻次
書目名稱Quantitative Methods in Proteomics被引頻次學(xué)科排名
書目名稱Quantitative Methods in Proteomics年度引用
書目名稱Quantitative Methods in Proteomics年度引用學(xué)科排名
書目名稱Quantitative Methods in Proteomics讀者反饋
書目名稱Quantitative Methods in Proteomics讀者反饋學(xué)科排名
作者: dialect 時間: 2025-3-21 20:17 作者: vibrant 時間: 2025-3-22 03:41
Quantitative Methods in Proteomics978-1-0716-1024-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 清真寺 時間: 2025-3-22 07:27 作者: Largess 時間: 2025-3-22 12:13
https://doi.org/10.1007/978-1-0716-1024-4Westernblotting; 2D-DIGE; iTRAQ; Plasma analysis,; Quantitative Secretomics; Proteomics作者: 辭職 時間: 2025-3-22 15:23 作者: dominant 時間: 2025-3-22 20:58
Important Issues in Planning a Proteomics Experiment : Statistical Considerations of Quantitative Pimes neglected part in detecting differential proteins is the statistical analysis. Data from proteomics experiments are usually high-dimensional and hence require profound statistical methods. It is especially important to already correctly design a proteomic experiment before it is conducted in th作者: Schlemms-Canal 時間: 2025-3-22 21:40
Good Old-Fashioned Protein Concentration Determination by Amino Acid Analysis,still this relevance within proteomics for multiple reasons especially in the quantitative protein analysis. Amino acid analysis enables indirectly both the protein and peptide concentration determination which are essential for using the same amounts for comparative quantitative experiments. Moreov作者: aggravate 時間: 2025-3-23 01:56
,Protein Quantification Using the “Rapid Western Blot” Approach,tion of a target protein based on the use of specific antibodies. However, the whole procedure is often very time-consuming. Nevertheless, with the development of fast blotting systems and further development of immunostaining methods, a reduction of the processing time can be achieved. Major challe作者: 詼諧 時間: 2025-3-23 09:01
The Whereabouts of 2D Gels in Quantitative Proteomics,teomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series. However, quantitative proteomics using two-dimensional gels is critically dependent on the performances of the protein dete作者: HATCH 時間: 2025-3-23 10:44
Proteome Analysis with Classical 2D-PAGE,on, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF). There are two important variants of IEF, which are carrier-ampholine (CA)-based IEF and immobilized pH-gradient (IPG)-based IEF. In the second dimension, proteins are further separated by their el作者: hemophilia 時間: 2025-3-23 15:55 作者: 虛情假意 時間: 2025-3-23 20:21 作者: ALIEN 時間: 2025-3-24 00:01 作者: Aggressive 時間: 2025-3-24 03:56 作者: justify 時間: 2025-3-24 08:56 作者: saturated-fat 時間: 2025-3-24 12:46 作者: Obligatory 時間: 2025-3-24 16:23
Quantitative Approach Using Matrix-Assisted Laser Desorption/ Ionization Time-of-Flight (MALDI-ToF)latforms are typically employed, as they provide highly reproducible outputs for batch processing of multiple samples. Quantitation using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF) mass spectrometry, while less commonly adopted, offers the ability to monitor analytes at作者: 脆弱吧 時間: 2025-3-24 22:26 作者: LIMN 時間: 2025-3-25 00:56 作者: 蔑視 時間: 2025-3-25 04:29
High-Throughput Profiling of Proteome and Posttranslational Modifications by 16-Plex TMT Labeling aure the dynamics of proteome with high throughput and deep coverage. The reproducibility of quantification benefits not only from the fascinating developments in high-performance liquid chromatography (LC) and high-resolution MS with enhanced scan rates but also from the invention of multiplexed iso作者: 招致 時間: 2025-3-25 11:19 作者: 欲望 時間: 2025-3-25 13:47 作者: entitle 時間: 2025-3-25 18:20
,2nSILAC for Quantitative Proteomics of Prototrophic Baker’s Yeast,arative analysis of (sub)proteomes. It is based on the metabolic incorporation of stable isotope-coded amino acids during growth of cells or organisms. Here, complete labeling of proteins with the amino acid(s) selected for incorporation needs to be guaranteed to enable accurate quantification on a 作者: 淡紫色花 時間: 2025-3-25 20:19 作者: Debark 時間: 2025-3-26 03:25
1064-3745 ation advice from the experts.This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative stud作者: Coronation 時間: 2025-3-26 06:43
Good Old-Fashioned Protein Concentration Determination by Amino Acid Analysis,n detergents or chaotropes are present in the sample buffer. Furthermore, it is highly sensitive. Nevertheless, amino acid analysis needs a certain experience to be set up and is time-consuming compared to other protein concentration determination techniques.作者: persistence 時間: 2025-3-26 09:29
An Approach for Triplex-IPTL,on of the proteins by endoproteinase Lys-C, followed by three combinations of selective dimethylation of the peptide N-termini and subsequent dimethylation of the lysine residues at the C-termini. Data analysis is performed using Mascot for database searches and the freely available software package IsobariQ for quantification.作者: 搖擺 時間: 2025-3-26 13:05 作者: conformity 時間: 2025-3-26 20:08
Application of SILAC Labeling in Phosphoproteomics Analysis,ing label-based quantification strategies the methods of choice in many experiments. Here, we present a protocol for SILAC labeling and the subsequent isolation of phosphopeptides using TiO. affinity chromatography. We outline the corresponding LC–MS/MS analysis and the essential steps of data processing.作者: Immortal 時間: 2025-3-26 22:06
Quantification and Identification of Post-Translational Modifications Using Modern Proteomics Approometry to be a high-throughput, label-free PTM quantification approach. Below we describe a detailed protocol to process tissue by homogenization and proteolytically digest proteins, followed by immunoaffinity enrichment of lysine-acetylated peptides to identify and quantify relative changes of acetylation comparing different conditions.作者: explicit 時間: 2025-3-27 04:33
Differential Proteome Analysis Using 2D-DIGE,s field. It provides multiplexing of up to three samples in one gel, higher sensitivity compared to normal protein staining methods, and a higher linear range for quantitation. This article gives detailed protocols for 2D-DIGE, including both minimal and saturation labeling.作者: 鴿子 時間: 2025-3-27 06:06
Book 2021Latest editionass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative studies, gel-based and mass spectrometry-based quantification techniques, TMT, IPTL, PRM, MALDI Imaging, SILAC, PTM analysis, DIA, cross-linking, and the up-to-date topics of software作者: Etching 時間: 2025-3-27 12:19 作者: 名字 時間: 2025-3-27 16:28
The Whereabouts of 2D Gels in Quantitative Proteomics,ction methods used after the electrophoretic separations. This chapter therefore examines critically the various detection methods, (radioactivity, dyes, fluorescence, and silver) as well as the data analysis issues that must be taken into account when quantitative comparative analysis of two-dimensional gels is performed.作者: Terrace 時間: 2025-3-27 19:37 作者: 倔強(qiáng)不能 時間: 2025-3-28 01:51
Quantitative Mass Spectrometry-Based Proteomics: An Overview,d finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.作者: Chemotherapy 時間: 2025-3-28 05:44
Tandem Mass Tags for Comparative and Discovery Proteomics,ex) to 10 samples (TMT10plex) and a nowadays of up to 16 samples (TMTpro 16plex). Here, we describe a straightforward protocol to perform relatively deep proteome quantitative analyses using TMT10plex.作者: 防銹 時間: 2025-3-28 08:13
Metabolic Labeling of , Proteins,ultivation in liquid .N-substituted medium followed by cultivation steps on solid .N-substituted medium. The described procedure results in a label incorporation rate higher than 97%. Cells prepared by the following method can be used as standard for relative quantification approaches of, e.g., the membrane or surface proteome of ..作者: 先鋒派 時間: 2025-3-28 14:18
l disease-orientated courses, by promoting new educational initiatives. One of these is the cloister seminars, short meetings intended for highly qualified oncologists and dealing with specific, controversial aspects of clinical practice and research. Another is the institution of permanent study gr作者: 內(nèi)閣 時間: 2025-3-28 17:02
Karin Schork,Katharina Podwojski,Michael Turewicz,Christian Stephan,Martin Eisenacheratment of cancer. There are two main reasons why such an initiative was considered necessary. Firstly, the teaching of oncology requires a rigorously multidiscipli- nary approach which is difficult for the Universities to put into practice since their system is mainly disciplinary orientated. Second作者: Boycott 時間: 2025-3-28 20:31 作者: TRACE 時間: 2025-3-29 01:56 作者: Suppository 時間: 2025-3-29 06:51 作者: Schlemms-Canal 時間: 2025-3-29 09:28
Caroline May,Frederic Brosseron,Kathy Pfeiffer,Kristin Fuchs,Helmut E. Meyer,Barbara Sitek,Katrin Male explored in Brazil.Skin accounts for approximately 15% of an adult’s total body weight, with a surface of about 2m2. It provides an entry and exit barrier to various substances, offers effective protection against harmful substances, microorganisms and ultraviolet radiation, and is also paramount作者: 慢慢啃 時間: 2025-3-29 12:02
Thierry Rabilloudle explored in Brazil.Skin accounts for approximately 15% of an adult’s total body weight, with a surface of about 2m2. It provides an entry and exit barrier to various substances, offers effective protection against harmful substances, microorganisms and ultraviolet radiation, and is also paramount作者: Guileless 時間: 2025-3-29 18:41
Caroline May,Frederic Brosseron,Piotr Chartowski,Kristin Fuchs,Helmut E. Meyer,Barbara Sitek,Katrin re is now more focus on measuring or estimating target unbound drug concentrations in the brain and evaluating the quantitative aspects of drug transport across the blood–brain barrier (BBB). The techniques for investigation of the rate and extent of BBB transport of new chemical entities (NCEs) are作者: 龍卷風(fēng) 時間: 2025-3-29 23:00 作者: Cardioplegia 時間: 2025-3-30 01:45 作者: 不給啤 時間: 2025-3-30 08:08 作者: 其他 時間: 2025-3-30 10:07
Katalin Barkovits,Weiqiang Chen,Michael Kohl,Thilo Brachts differences, in vitro/in vivo differences, age-related differences, and normal/disease differences, and these need to be understood in order to guide rational drug discovery and development. To address this issue, we have developed an absolute quantification method of protein expression, called qu作者: Pelago 時間: 2025-3-30 16:08 作者: HARD 時間: 2025-3-30 18:20 作者: habitat 時間: 2025-3-30 21:31 作者: ADORE 時間: 2025-3-31 02:46
Kaiwen Yu,Zhen Wang,Zhiping Wu,Haiyan Tan,Ashutosh Mishra,Junmin Pengracted much attention through the years as it holds a promise that this drug property can be predicted in silico from the chemical drug structure. Various in vivo methodologies have been used to define and quantify drug exposure in the brain, the most commonly used parameter being logBB, which is th作者: infelicitous 時間: 2025-3-31 09:01
Anja Holtz,Nathan Basisty,Birgit Schillingre is now more focus on measuring or estimating target unbound drug concentrations in the brain and evaluating the quantitative aspects of drug transport across the blood–brain barrier (BBB). The techniques for investigation of the rate and extent of BBB transport of new chemical entities (NCEs) are作者: 退潮 時間: 2025-3-31 11:21
Thilo Werner,Michael Steidel,H. Christian Eberl,Marcus Bantschefffunction may be parlayed into meaningful disease applications. The development of impactful human therapies is a difficult and lengthy process; nonetheless, many researchers desire to see their work applied to the treatment of human disease. Significant financial reward will follow functional soluti作者: Musculoskeletal 時間: 2025-3-31 15:09 作者: 不自然 時間: 2025-3-31 18:12
Anke Trautwein-Schult,Jürgen Bartel,Sandra Maa?,D?rte Bechery and immortalized brain endothelial cells have been described, issues regarding the retention of BBB properties and scalability have hampered their use for certain applications. Differentiation of stem cells to endothelial cells exhibiting BBB properties offers advantages including high barrier, hu作者: 實現(xiàn) 時間: 2025-4-1 00:28
e subjects discussed. It Wa& decided to keep the layout very simple in order to keep costs to a minimum and make the monographs available in the shortest possible time, thus overcoming a common problem in medical literature: that of the material being outdated even before publication.978-3-642-73079-5978-3-642-73077-1作者: 有偏見 時間: 2025-4-1 01:50
Karin Schork,Katharina Podwojski,Michael Turewicz,Christian Stephan,Martin Eisenachertherapy, radiotherapy alone or integrated), it is the ambition of the European School of Oncology to fill a cultural and scientific gap and, thereby, create a bridge between the University and Industry and between these two and daily medical practice. One of the more recent initiatives of ESO has be作者: 背景 時間: 2025-4-1 06:28
Caroline May,Bettina Serschnitzki,Katrin Marcusemotherapy, radiotherapy alone or integrated), it is the ambition of the European School of Oncology to fill a cultural and scientific gap and, thereby, create a bridge between the University and Industry and between these two and daily medical practice. One of the more recent initiatives of ESO has作者: FIS 時間: 2025-4-1 12:00
Katalin Barkovits,Kathy Pfeiffer,Britta Eggers,Katrin Marcustful or “marginally useful” [1]. They are responsible for many side-effects, unrealistic expectations of regaining former vitality and frequent hospitalisation. Furthermore, this therapeutical attitude contributes a great deal to the rising costs of medical intervention. The ever-increasing expendit作者: IVORY 時間: 2025-4-1 15:35
