標(biāo)題: Titlebook: p53 Protocols; Sumitra Deb,Swati Palit Deb Book 2003 Humana Press 2003 [打印本頁] 作者: Deleterious 時間: 2025-3-21 16:32
書目名稱p53 Protocols影響因子(影響力)
書目名稱p53 Protocols影響因子(影響力)學(xué)科排名
書目名稱p53 Protocols網(wǎng)絡(luò)公開度
書目名稱p53 Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱p53 Protocols被引頻次
書目名稱p53 Protocols被引頻次學(xué)科排名
書目名稱p53 Protocols年度引用
書目名稱p53 Protocols年度引用學(xué)科排名
書目名稱p53 Protocols讀者反饋
書目名稱p53 Protocols讀者反饋學(xué)科排名
作者: finite 時間: 2025-3-21 23:46 作者: GET 時間: 2025-3-22 02:00
Melissa L. Dumble,Lawrence A. Donehower,Xiongbin Lueit scheinen nicht vorhanden. Und tats?chlich: Logistische Prinzipien vereinen Effektivit?ts- und Effizienzdenken, stetiger W- del ist zu antizipieren, neue Konzepte sind an Kundenanforderungen anzupassen. Logistik und IT determinieren in Industrie und Handel den Erfolg des Kerngesch?fts der Unterne作者: 詞匯記憶方法 時間: 2025-3-22 05:28
Susanne Stein,Yong-Jig Cho,Roger S. Jackson II,Peng Lianggen. In diesem Kapitel steht die erste Forschungsfrage im Vordergrund: Inwieweit ist in der ?ffentlichen Verwaltung eine integrative und ganzheitliche Handhabung physischer und digitaler Dokumente von Bedeutung? Dazu werden zuerst in Kapitel 2.1 allgemein die Charakteristika der ?ffentlichen Verwalt作者: Mutter 時間: 2025-3-22 11:24 作者: Meander 時間: 2025-3-22 13:38
Maria Patricia Molina,Christine Cain,Jill Bargonettior- und nachgelagerten Gesch?ftsprozessen (z.B. industrielle Anfrage-/Angebotsabwicklung) gilt. Dies drückt sich in den vorrangigen Zielen vieler Unternehmen aus, Durchlaufzeiten und Proze?kosten solcher Gesch?ftsabl?ufe zu verringern sowie ihre Termintreue zu steigern [z.B. FIR94, VDI94]. Veranscha作者: 危險 時間: 2025-3-22 20:15
Michael Berger,Ygal Hauptnge rates. These movements have been accompanied by large swings in real exchange rates, reflecting the fact that nominal exchange rate variations have not closely followed changes in relative prices of traded goods. The short-run variability of exchange rates — whether measured in real or nominal t作者: 使激動 時間: 2025-3-22 22:47 作者: 發(fā)出眩目光芒 時間: 2025-3-23 02:56 作者: 進步 時間: 2025-3-23 09:15
Sumitra Deb,Swati Palit DebIncludes supplementary material: 作者: 總 時間: 2025-3-23 11:25 作者: ABASH 時間: 2025-3-23 16:28
Book 2003this protein. Several unique properties of p53 posed a challenge to understa- ing its normal function in the initial phase of its research. The low levels of p53 in normal cells, its stabilization under situations of genotoxic stress, induction of growth arrest, and apoptosis with stabilization of t作者: evanescent 時間: 2025-3-23 19:07 作者: commonsense 時間: 2025-3-23 23:35
Purification of Recombinant p53 from Sf9 Insect Cells,h with no flanking sequences and its expression is driven by the baculovirus polyhedron promoter. We also describe how to concentrate the p53 up to 0.9 mg/mL. By gel filtration analysis, we demonstrate that 20% of the p53 forms a tetramer, and 80% forms a monomer. In a DNA binding assay known as the作者: 泛濫 時間: 2025-3-24 05:42 作者: Prologue 時間: 2025-3-24 09:10 作者: reperfusion 時間: 2025-3-24 10:54 作者: BURSA 時間: 2025-3-24 15:06
,Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR? Green I,nalysis as a target of p53 transrepression and mutant p53 transactivation. A p53-null cell line derived from lung carcinoma, H1299, was infected with recombinant adenovirus expressing wild-type (WT) p53, mutant p53-D281G, or β-galactosidase as a control. After 24 h of infection, RNA was harvested an作者: FLAIL 時間: 2025-3-24 19:10
Transactivation and Transrepression Studies with p53, and p73). Once a gene of interest is identified, its presumptive promoter region can be cloned upstream of a luciferase gene in a plasmid. The most common reason for transfection experiments is to study gene expression patterns in the presence or absence of a particular gene product (e.g., p53). Th作者: 變化 時間: 2025-3-25 01:00
Methods to Study p53-Repressed Promoters,n also function as a sequence-specific transcriptional repressor of a separate set of genes. However, elucidation of the mechanism whereby p53 functions as a transcriptional repressor has been obscured by the use of artificial assays to measure this activity; these assays include transient transfect作者: Concerto 時間: 2025-3-25 05:08 作者: 幻想 時間: 2025-3-25 09:00
Interaction of p53 with the Adenovirus E1B-55 kDa Protein,raction of E1B-55 kDa with p53; (.) inhibition of p53-induced transcription; (.) relocalization of p53 from the nucleus to the cytoplasm; and (.) in the simultaneous presence of E1B-55 kDa and the adenovirus E4-34 kDa (E4orf6) protein, extensive destabilization of p53. These activities can be observ作者: cardiopulmonary 時間: 2025-3-25 12:41 作者: muffler 時間: 2025-3-25 17:29 作者: eucalyptus 時間: 2025-3-25 23:48 作者: BRAVE 時間: 2025-3-26 01:26
Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress, nuclear export of p53 via the CRM1 pathway. However, proteasomes are abundant in both cytosol and nucleus. We recently analyzed HDM2-mediated degradation of endogenous p53 in the presence of various CRM1 blockers. We found that significant HDM2-mediated degradation takes place despite nuclear expor作者: micturition 時間: 2025-3-26 05:57
Mutational Analysis of p53 in Human Tumors,d from the tumor samples and then used as a template to amplify the p53 coding sequences by polymerase chain reaction (PCR). The PCR products are characterized by agarose gel electrophoresis. Subsequently, mutations can be detected by direct DNA sequencing of the PCR products, followed by acrylamide作者: 貨物 時間: 2025-3-26 10:01 作者: Flatus 時間: 2025-3-26 12:38 作者: 其他 時間: 2025-3-26 19:33 作者: SAGE 時間: 2025-3-26 23:01 作者: Liability 時間: 2025-3-27 02:51
Transactivation and Transrepression Studies with p53,e first method is ideal for the study of transactivation and transrepression properties of p53 (or other transcription factors). The last two are more suited for experiments where larger numbers of transfected cells are needed. Several examples of transfections and their respective results are provided.作者: 我沒有命令 時間: 2025-3-27 06:55
Interaction of p53 with Cellular Proteins,not require specialized expertise to establish in the laboratory, give reliable and reproducible data, and are cost-efficient, because few reagents are needed. This chapter gives a basic description of these two techniques and provides tips that are not found in other protocol manuals on how to achieve the best results.作者: Orgasm 時間: 2025-3-27 13:23 作者: Hot-Flash 時間: 2025-3-27 15:16 作者: 單片眼鏡 時間: 2025-3-27 20:04
Purification of Recombinant p53 from Sf9 Insect Cells,9 mg/mL. By gel filtration analysis, we demonstrate that 20% of the p53 forms a tetramer, and 80% forms a monomer. In a DNA binding assay known as the electromobility shift assay, the purified p53/DNA complex forms a single band the gel. This simple procedure should be useful for investigations into the biochemistry of the p53 protein.
