標(biāo)題: Titlebook: Opioid Research; Methods and Protocol Zhizhong Z. Pan Book 2003 Humana Press 2003 [打印本頁(yè)] 作者: HAND 時(shí)間: 2025-3-21 16:56
書目名稱Opioid Research影響因子(影響力)
書目名稱Opioid Research影響因子(影響力)學(xué)科排名
書目名稱Opioid Research網(wǎng)絡(luò)公開度
書目名稱Opioid Research網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Opioid Research被引頻次
書目名稱Opioid Research被引頻次學(xué)科排名
書目名稱Opioid Research年度引用
書目名稱Opioid Research年度引用學(xué)科排名
書目名稱Opioid Research讀者反饋
書目名稱Opioid Research讀者反饋學(xué)科排名
作者: MAIZE 時(shí)間: 2025-3-21 21:06
Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cellsse is commonly exploited to study the responsiveness to opioid ligands at the cellular level. In most of the cell systems studied, acute activation of opioid receptors leads to inhibition of adenylyl cyclase activity and a decrease in intracellular cAMP levels (.).作者: Defraud 時(shí)間: 2025-3-22 02:31
Immunohistochemical Localization of μ-, δ- and K-Opioid Receptors Within the Antinociceptive Brainsttinociception. It has been hypothesized that the analgesic effects of opiates are mediated indirectly through inhibition of inhibitory GABAergic neurons within the PAG-RVM-spinal cord circuit (.,.–.).作者: Allodynia 時(shí)間: 2025-3-22 05:04 作者: 隱士 時(shí)間: 2025-3-22 11:20
Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate charged phosphate group alters the structure of the protein and regulates its functional properties. Protein phosphorylation is a highly regulated reversible process and thus the level of receptor phosphorylation in neurons is constantly modified by neurotransmitters and other cellular signals released by surrounding neurons.作者: Admire 時(shí)間: 2025-3-22 16:17
Hybridization in Neural Tissuese or nonradioactive techniques and quantitated using specialized image analysis programs. This chapter will describe a sensitive radioactive method of . hybridization, with references given to other variants in technique.作者: 預(yù)防注射 時(shí)間: 2025-3-22 20:47
Opioid Receptor Oligomerizationby the activation of multiple signal transduction pathways including the inhibition of adenylyl cyclase, modulation of inwardly rectifying K. channels or voltage-dependent calcium channels and regulation of mitogen-activated protein (MAP) kinase activity (.–.).作者: compose 時(shí)間: 2025-3-22 21:23 作者: Inexorable 時(shí)間: 2025-3-23 04:39 作者: 無(wú)辜 時(shí)間: 2025-3-23 05:54 作者: mitten 時(shí)間: 2025-3-23 12:56
Opioid Research978-1-59259-379-8Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: tackle 時(shí)間: 2025-3-23 16:03
Methods in Molecular Medicinehttp://image.papertrans.cn/o/image/702429.jpg作者: 圓錐體 時(shí)間: 2025-3-23 20:37
Receptor Knock-Out and Gene Targetingnterpretation of the experimental data was complicated because of the poor selectivity of opioid compounds. The precise contribution of each receptor to the effects of opioid drugs remained to be elucidated.作者: APEX 時(shí)間: 2025-3-24 01:13 作者: Demonstrate 時(shí)間: 2025-3-24 05:14 作者: 柔軟 時(shí)間: 2025-3-24 06:50
Expression of Opioid Receptors in Mammalian Cell Linesied by molecular cloning. Although each of the cloned opioid receptors is derived from a single gene, a number of alternatively spliced variants from their own genes have been isolated (.–.). One extraordinary example is the mouse μ opioid receptor (.) gene in which alternative splicing of the fourt作者: Cerebrovascular 時(shí)間: 2025-3-24 12:00
Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cellsceptors, translate extracellular messages into cellular function. Following receptor activation, adenylyl cyclase is either activated or inhibited via the α-subunit of G. or G. protein, respectively (.). Regulation of adenylyl cyclase activity consequently leads to changes in intracellular levels of作者: Constant 時(shí)間: 2025-3-24 17:22
Analysis of Opioid-Induced Kinase Activationtalyze phosphorylation of a protein and transfer the γ-phosphate of adenosine triphosphate (ATP) onto the serine, threonine, or tyrosine residue. It has been shown that stimulation of opioid receptors regulates activities of numerous protein kinases including protein kinase C (PKC), cAMP-dependent p作者: 樸素 時(shí)間: 2025-3-24 22:37 作者: FLOAT 時(shí)間: 2025-3-24 23:30
Opioid Receptor Coupling to GIRK Channels3), adenylyl cyclases, and voltage-dependent calcium channels (.). GIRK channels have been shown to be involved in opioid-induced analgesia (.). These channels are activated by G protein-coupled receptors (GPCRs) such as opioid, nociceptin/orphanin FQ, M2 muscarinic, α. adrenergic, and D. dopaminerg作者: 調(diào)情 時(shí)間: 2025-3-25 05:19
Identification of Alternatively Spliced Variants from Opioid Receptor Genesernative splicing of its 14 exons (.–.). Furthermore, the region-specific expression of the splice variants suggests an important role of alternative splicing in regulating gene functions (.–.). Genomic sequences from various species are available from many genome projects, but it is still challengi作者: 昏暗 時(shí)間: 2025-3-25 09:03 作者: 傾聽 時(shí)間: 2025-3-25 15:13
Hybridization in Neural TissuesNA (mRNA) of interest in its neuroanatomical context and in a relatively quantitative manner. . hybridization can be performed using either radioactive or nonradioactive techniques and quantitated using specialized image analysis programs. This chapter will describe a sensitive radioactive method of作者: Respond 時(shí)間: 2025-3-25 17:24
Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genesnd PCR confuse the interpretation of results. This chapter demonstrates three refinements of the standard RT-PCR strategy, which together provide explicit measurements of single-cell gene expression in terms of mRNA molecules per cell. (1) The entire cell is harvested and reverse transcribed. (2) Mu作者: 伸展 時(shí)間: 2025-3-25 22:56
Gene Arrays and Proteomicser to control cellular functions. In the opioid field, we concern ourselves both with signaling mechanisms within cells and the functions of neural circuitry in mediating the behavioral effects of opioids. All of these mechanisms identified to date have proven to be extremely complex, suggesting tha作者: EVADE 時(shí)間: 2025-3-26 02:47 作者: 比喻好 時(shí)間: 2025-3-26 04:17 作者: Conclave 時(shí)間: 2025-3-26 11:21 作者: minimal 時(shí)間: 2025-3-26 15:55
Pharmacological Interventions at the Spinal Cord some of the intrinsic spinal circuitry underlying spinal reflexes. To understand the modulation of spinal processing by local spinal innervations and descending fibers from supraspinal structures to the spinal cord, a pharmacological approach is often more useful than a surgical one. The developmen作者: 蜿蜒而流 時(shí)間: 2025-3-26 20:26
Opioid Tolerance in Adult and Neonatal Ratshe most effective analgesics and are widely used in current clinical management of various pain conditions. They are particularly required to chronically treat severe and persistent pain in patients suffering from chronic diseases, such as cancer. Repeated use of opioid drugs, however, induces the d作者: electrolyte 時(shí)間: 2025-3-27 00:44
reiheit ‘84 — Macht oder Ohnmacht der Medien?” Der Saal war überfüllt. Auf dem Podium sa?en Politiker, Journalisten, Wissenschaftler. Rechts neben mir der damalige Korrespondent des ZDF in Paris, Horst Sch?ttle. Wir langweilten uns und begannen ein Gespr?ch über mein Lieblingsthema zur damaligen Zei作者: Formidable 時(shí)間: 2025-3-27 01:16 作者: 神圣將軍 時(shí)間: 2025-3-27 07:16 作者: flaggy 時(shí)間: 2025-3-27 11:45 作者: Inoperable 時(shí)間: 2025-3-27 17:32
Zhizhong Z. Pant der Problematik, die bedingungslose Gewinnerzielung, wird offensichtlich von Milton Friedman. in dessen viel zitiertem Aufsatz ?The Social Responsibility of Business is to Increase its Profits“ markiert. Danach haben Unternehmen ausschlie?lich die Verantwortung, sich am Markt zu bew?hren und ihren作者: CANON 時(shí)間: 2025-3-27 21:07
Molecular Cloning of Opioid Receptors by cDNA Library Screeningd probe is ideal for screening its homologs, or associated splicing variants or full-length cDNAs. If only a partial protein sequence is on hand, degenerate primers can be designed to screen cDNA libraries with a direct polymerase chain reaction (PCR) or with a hybridization procedure. Alternatively作者: Notify 時(shí)間: 2025-3-28 01:29
Analysis of Opioid-Induced Kinase Activationoid signaling to other signal pathways. Furthermore, protein kinases activated by nonopioid signal pathways play important roles in heterologous regulation of opioid functions. Therefore, opioid researchers often face the challenge of determining changes in the activities of protein kinases in study作者: 偏狂癥 時(shí)間: 2025-3-28 04:32 作者: ALOFT 時(shí)間: 2025-3-28 08:27 作者: MOCK 時(shí)間: 2025-3-28 14:24
Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genesces illustrates this approach. In the case of GPD, RT yields one amplifiable cDNA molecule for every 2–3 mRNA molecules. Sensory neurons maintain GPD mRNA in the nanomolar range, but with considerable variability (1.03±0.61 n.). Competitive PCR can be applied to virtually any message sequence. In ne作者: Angiogenesis 時(shí)間: 2025-3-28 15:32 作者: 貞潔 時(shí)間: 2025-3-28 22:49
Recombinant Opioid Receptorsl tail. Comparison of their deduced protein sequences revealed that opioid receptors are about 60% identical, with the greatest identity found in the transmembrane domains (73–76%) and intracellular loops (86–100%) (.). Despite these similarities, δ, μ, and κ opioid receptors bind specific ligands w作者: 鍍金 時(shí)間: 2025-3-29 01:58 作者: 意外的成功 時(shí)間: 2025-3-29 04:56 作者: chassis 時(shí)間: 2025-3-29 09:57
Deepak R. Thakker,Hatice Z. Ozsoy,Kelly M. Standifer作者: Neutropenia 時(shí)間: 2025-3-29 13:19 作者: 小官 時(shí)間: 2025-3-29 16:35 作者: CRAB 時(shí)間: 2025-3-29 20:42
tischen Standort gefragt.. Sie kam zu dem gleichen Ergebnis wie die Allensbacher Umfrage. Von politischer Ausgewogenheit der deutschen Medien k?nne demnach nicht die Rede sein, argumentierte ich. “Das überzeugt mich nicht”, sagte Sch?ttle. “Warum untersuchen Sie nicht lieber den . der Medien, auf de作者: Inculcate 時(shí)間: 2025-3-30 01:11
Ying-Xian Pangarien, Polen und Ungarn bei der offiziellen Staatsbezeichnung nie über das Stadium der ‘Volksrepublik’ hinauskamen; Polens Aufstieg wurde durch das teilweise noch existierende Privateigentum an Grund und Boden. sowie durch eine politische Struktur, die Züge eines Mehrparteiensystems trug,. blockier作者: 背叛者 時(shí)間: 2025-3-30 07:10
Ying-Xian Pangarien, Polen und Ungarn bei der offiziellen Staatsbezeichnung nie über das Stadium der ‘Volksrepublik’ hinauskamen; Polens Aufstieg wurde durch das teilweise noch existierende Privateigentum an Grund und Boden. sowie durch eine politische Struktur, die Züge eines Mehrparteiensystems trug,. blockier作者: Ardent 時(shí)間: 2025-3-30 08:36 作者: 露天歷史劇 時(shí)間: 2025-3-30 12:54
Zhizhong Z. Pannische Ziele. Diese Feststellung wird auch von Vertretern der Finanzindustrie bejaht, wobei es nicht immer leicht f?llt, Lippenbekenntnisse und von der Mode beeinflusste ?u?erungen einerseits von echter Verantwortung getragenem Verhalten andererseits zu differenzieren. Immerhin finden sich heute ver作者: perimenopause 時(shí)間: 2025-3-30 17:31 作者: 熔巖 時(shí)間: 2025-3-30 22:51 作者: 大酒杯 時(shí)間: 2025-3-31 03:45
in) had a broad spectrum of carcinogenicity affecting a variety of organs. Several agents led to tumors in the hematopoietic and lymphatic systems in mice or rats or both. The correlation to the general immunosuppressive action is not quite clear. 6-Mercaptopurine induces lymphosarcomas in mice.作者: 瑪瑙 時(shí)間: 2025-3-31 08:40
Construction of a Digital SHG: Two Examples,In the previous chapter the various components required for an SHG build were discussed in detail. In this chapter, a couple of design concepts are presented which could be easily constructed by the amateur and then combine these solutions with the option of using two different imaging cameras.作者: Anal-Canal 時(shí)間: 2025-3-31 11:50 作者: 有花 時(shí)間: 2025-3-31 17:14
