標(biāo)題: Titlebook: Oogenesis; Methods and Protocol Ioannis P. Nezis Book 2016 Springer Science+Business Media New York 2016 plants.oogenesis.mosquitos.chicken [打印本頁(yè)] 作者: 多愁善感 時(shí)間: 2025-3-21 18:42
書(shū)目名稱(chēng)Oogenesis影響因子(影響力)
作者: Creditee 時(shí)間: 2025-3-21 21:02 作者: Resection 時(shí)間: 2025-3-22 03:58 作者: 男學(xué)院 時(shí)間: 2025-3-22 05:20
Analysis of Phagocytosis in the , Ovary,gulfing follicle cells. . strains expressing GFP and mCherry protein fusions can enable a detailed analysis of phagosome maturation. LysoTracker labels highly acidified compartments, marking phagolysosomes. Together these labels can be used to mark the progression of engulfment in . follicle cells.作者: IRK 時(shí)間: 2025-3-22 11:55 作者: Agnosia 時(shí)間: 2025-3-22 14:15 作者: Fortify 時(shí)間: 2025-3-22 19:27
RNA FISH, DNA FISH and Chromosome Painting of Chicken Oocytes,e describe in detail the protocols for FISH based techniques applied to study gene expression dynamics and nuclear architecture of chicken oocytes during meiotic prophase I. These techniques can be easily performed in any molecular and cell biology laboratory and be adapted to different systems and 作者: ADORE 時(shí)間: 2025-3-22 23:29 作者: 生來(lái) 時(shí)間: 2025-3-23 01:57 作者: dyspareunia 時(shí)間: 2025-3-23 08:45 作者: dandruff 時(shí)間: 2025-3-23 09:52 作者: BLANC 時(shí)間: 2025-3-23 17:00
Nathaniel C. Peters,Celeste A. Bergregern. Die Barriere Hornschicht wird durch Insektenstiche überspielt. Anflug- und Landeorgane der Insekten haben sich bereits so an die menschliche Haut adaptiert, da? Stich- oder Bi?reaktionen im gesamt?kologischen System ihren Platz haben. Der rote Faden, der sich durch das Thema: die Haut als Ei作者: 飛鏢 時(shí)間: 2025-3-23 19:20 作者: 可互換 時(shí)間: 2025-3-24 01:14
1064-3745 d tips on troubleshooting andavoiding known pitfalls...Authoritative and cutting-edge, .Oogenesis:Methods and Protocols. aims to ensure successful results in the furtherstudy of this vital field..978-1-4939-8141-0978-1-4939-3795-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: cyanosis 時(shí)間: 2025-3-24 06:11 作者: heterogeneous 時(shí)間: 2025-3-24 06:56 作者: 北極人 時(shí)間: 2025-3-24 11:51
Jean-Michel Carter,Melanie Gibbs,Casper J. Breuker作者: 試驗(yàn) 時(shí)間: 2025-3-24 16:31 作者: 打火石 時(shí)間: 2025-3-24 22:07
Studying Oogenesis in a Non-model Organism Using Transcriptomics: Assembling, Annotating, and Analyof the operations in the primary annotation pipeline can be performed in Galaxy (or equivalent standalone versions of the tools) and through the use of common database operations (e.g. to remove duplicates) but other equivalent programs and/or custom scripts can be implemented for further automation.作者: enchant 時(shí)間: 2025-3-24 23:18 作者: antecedence 時(shí)間: 2025-3-25 03:19
Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting,of the proteins Ddx4 or Ifitm3, alone or in combination with the use of fluorescent reporter mice. Beginning with sample preparation, we provide point-by-point instructions to guide researchers on how to isolate OSCs using flow cytometry.作者: 小蟲(chóng) 時(shí)間: 2025-3-25 10:25 作者: 彩色 時(shí)間: 2025-3-25 15:42
Immuno-Gold Labeling of , Follicles for Transmission Electron Microscopy,ples, which focus at the retention of antigenicity in efforts to mirror the native state of the tissues and cells as closely as possible. Herein, we describe the methodology for immuno-gold labeling of . follicles, following preparation of the samples using the Tokuyasu method for ultracryosectioning.作者: FRET 時(shí)間: 2025-3-25 17:35 作者: 災(zāi)禍 時(shí)間: 2025-3-25 23:29 作者: 小平面 時(shí)間: 2025-3-26 00:18
Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes,oinjection, the meiotic dynamics of intracellular structures, such as chromosomes, can be monitored at high resolution. A combination of this method with approaches for the perturbation of specific proteins opens up opportunities for understanding the molecular and intracellular basis of mammalian meiosis.作者: GUMP 時(shí)間: 2025-3-26 04:47
Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes,re we describe several reliable methods for microtubule and/or microfilaments preservation and visualization in . oocyte extracts, and in situ in live and fixed insect and frog (.) oocytes. In addition, we describe visualization of cytoskeleton-bound RNAs using molecular beacons in live . oocytes.作者: 全國(guó)性 時(shí)間: 2025-3-26 08:47
Book 2016pective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls...Authoritative and cutting-edge, .Oogenesis:Methods and Protocols. aims to ensure successful results in the furtherstudy of this vital field..作者: PAGAN 時(shí)間: 2025-3-26 13:42 作者: 惹人反感 時(shí)間: 2025-3-26 16:48 作者: 敲詐 時(shí)間: 2025-3-27 00:27 作者: 哀悼 時(shí)間: 2025-3-27 04:20 作者: 極小 時(shí)間: 2025-3-27 05:20
John L. Brenner,Tim Schedlns for pituitary adenomas performed by Horsley in 1889 (Horsley, 1906), who used the transfrontal and transtemporal approaches and by Schloffer in 1907, who performed the first transnasal intervention. The challenge has been manyfold. For the surgeon it was mainly the formidable localization of the 作者: 笨拙的我 時(shí)間: 2025-3-27 13:09 作者: 有雜色 時(shí)間: 2025-3-27 17:07 作者: Decrepit 時(shí)間: 2025-3-27 21:22
Anne-Claire Jacomin,Ioannis P. Nezis der Funktion einer partiell holokrinen Drüse zu vergleichen ist. Der Schwerpunkt der Barrierefunktion der Haut gegenüber Fremdstoffen liegt im unteren Anteil der Hornschicht. Der passive Durchtritt von Mikroben ist durch die v?llig intakte Haut nicht m?glich. Es mu? aber zugestanden werden, da? der作者: triptans 時(shí)間: 2025-3-28 00:44
Histological Analysis of the , Gynoecium and Ovules Using Chloral Hydrate Clearing and Differentialveloping plant organs without the need for paraffin embedding and sectioning. This approach is appropriate for the analysis of the developing gynoecium or seedpod of the flowering plant . and many other types of fixed plant material. Early stages of ovule development are observable with this approac作者: machination 時(shí)間: 2025-3-28 06:03 作者: 的染料 時(shí)間: 2025-3-28 09:35
Antibody Staining in , Germaria,ure called the germarium, which harbors the stem cells for both germ and somatic cells. The germarium produces egg chambers, each of which will develop into an egg. Active areas of research in . germaria include stem cell self-renewal, division, and maintenance, cell cycle control and differentiatio作者: lacrimal-gland 時(shí)間: 2025-3-28 12:12 作者: –DOX 時(shí)間: 2025-3-28 16:28 作者: Instinctive 時(shí)間: 2025-3-28 19:55 作者: temperate 時(shí)間: 2025-3-28 23:33 作者: overreach 時(shí)間: 2025-3-29 05:22
Preparation of , Follicles for Transmission Electron Microscopy,res. This is essential for understanding a large variety of cellular processes. In this chapter, we describe a detailed protocol for preparing . follicles in order to be used as a specimen for transmission electron microscopy.作者: Malfunction 時(shí)間: 2025-3-29 10:00
Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles,archers can use either the relative or absolute (standard curve) method to quantify transcript abundance. Characterizing the expression of genes in mosquito ovaries will require use of the standard curve method of quantification. Here, I describe reagents and equipment necessary to run standard curv作者: 親屬 時(shí)間: 2025-3-29 14:36 作者: 長(zhǎng)矛 時(shí)間: 2025-3-29 17:23 作者: 魔鬼在游行 時(shí)間: 2025-3-29 20:23
High-Pressure Freezing Electron Microscopy of Zebrafish Oocytes,ous animals are enormous cells endowed with many, big cellular compartments, which are interconnected through active intracellular transport. The dynamic transport pathways and the big organelles of the oocyte provide the opportunity to study cellular trafficking with outstanding resolution. Hence, 作者: 公共汽車(chē) 時(shí)間: 2025-3-30 00:35
Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes,rious structures and molecules are temporarily or permanently bound to the cytoskeletal elements and their functions rely on cytoskeleton integrity and its timely assembly. Thus the accurate visualization of cytoskeleton is often crucial for studies and analyses of oocyte structure and functions. He作者: Digest 時(shí)間: 2025-3-30 06:23 作者: 冒失 時(shí)間: 2025-3-30 08:49
Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis,ons in collecting sufficient numbers of oocytes can hinder the efficiency of the technique. Here we provide a detailed protocol for the accurate preparation of mouse oocyte samples for Western Blotting analysis.作者: 歌曲 時(shí)間: 2025-3-30 12:55 作者: 魅力 時(shí)間: 2025-3-30 17:08
Monitoring Calcium Oscillations in Fertilized Mouse Eggs,m. The monitoring of these Ca. oscillations is a valuable technique to demonstrate that fertilization has occurred, to study egg activation events elicited downstream of the Ca. signal, as well as to evaluate sperm quality. This chapter describes our protocol to monitor sperm-induced Ca. oscillation作者: finite 時(shí)間: 2025-3-30 22:33
Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes,onfocal live imaging to mouse oocytes, in which meiotic maturation can be induced in vitro after the introduction of fluorescent proteins through microinjection, the meiotic dynamics of intracellular structures, such as chromosomes, can be monitored at high resolution. A combination of this method w作者: 使人入神 時(shí)間: 2025-3-31 03:24
Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting,Cs) or adult female germline stem cells (aFGSCs), has provided a robust system to study female germ cell development under multiple experimental conditions, and in many species. Flow cytometry or fluorescence-activated cell sorting (FACS) is an integral part of many isolation and characterization pr作者: Biomarker 時(shí)間: 2025-3-31 08:08
Ioannis P. NezisIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 男生如果明白 時(shí)間: 2025-3-31 13:14 作者: 中子 時(shí)間: 2025-3-31 17:06
Preparation of , Follicles for Transmission Electron Microscopy,res. This is essential for understanding a large variety of cellular processes. In this chapter, we describe a detailed protocol for preparing . follicles in order to be used as a specimen for transmission electron microscopy.作者: Lamina 時(shí)間: 2025-3-31 21:24
Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis,ons in collecting sufficient numbers of oocytes can hinder the efficiency of the technique. Here we provide a detailed protocol for the accurate preparation of mouse oocyte samples for Western Blotting analysis.作者: 松馳 時(shí)間: 2025-4-1 01:12 作者: Intervention 時(shí)間: 2025-4-1 05:48
Oogenesis978-1-4939-3795-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Living-Will 時(shí)間: 2025-4-1 06:09
Methods in Molecular Biologyhttp://image.papertrans.cn/o/image/701750.jpg作者: 凌辱 時(shí)間: 2025-4-1 10:55 作者: 服從 時(shí)間: 2025-4-1 14:27
der Region zielt. Die Lasten der Vergangenheit und die ungünstigen Ausgangsbedingungen im Südraum Leipzig, einer ?kologisch hochbelasteten l?ndlichen Bergbau- und Industrieregion, verlangen eine Prüfung, ob das Leitbild einer nachhaltigen Entwicklung für diese Region überhaupt tragf?hig und anwendb
