標題: Titlebook: Nucleic Acid and Peptide Aptamers; Methods and Protocol Günter Mayer Book 2009 Humana Press 2009 Ligands.Protein modification.RNA.Target mo [打印本頁] 作者: exposulate 時間: 2025-3-21 18:48
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書目名稱Nucleic Acid and Peptide Aptamers讀者反饋學科排名
作者: pulse-pressure 時間: 2025-3-21 21:06 作者: 紅腫 時間: 2025-3-22 01:25 作者: 發(fā)電機 時間: 2025-3-22 04:40
Beatrix Suess,Julia E. Weigands show variety in the employed methods, from multivariate anIn ‘The arena of everyday life‘ nine authors look back and forward at developments in the sociology of consumers and households. Nine chapters show variety in the employed methods, from multivariate analyses of survey data to classical essa作者: 凝乳 時間: 2025-3-22 08:47 作者: 一瞥 時間: 2025-3-22 15:28 作者: 角斗士 時間: 2025-3-22 20:51 作者: accrete 時間: 2025-3-22 22:48 作者: Morbid 時間: 2025-3-23 02:40 作者: 平息 時間: 2025-3-23 06:07 作者: 得罪 時間: 2025-3-23 11:58 作者: Aggressive 時間: 2025-3-23 14:06
In Vitro Selection of ssDNA Aptamers Using Biotinylated Target ProteinsAptamers have been described for a series of target molecules including peptides, proteins, and even living cells. Besides RNA and 2′-modified RNA molecules also ssDNA molecules can be subjected to in vitro selection protocols aiming at the enrichment of ssDNA aptamers. ssDNA aptamers can be selecte作者: 芭蕾舞女演員 時間: 2025-3-23 18:39 作者: cravat 時間: 2025-3-24 00:25 作者: 老巫婆 時間: 2025-3-24 04:25 作者: creatine-kinase 時間: 2025-3-24 09:35 作者: 粗鄙的人 時間: 2025-3-24 11:50
Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interactionons based on a change of diffusion times (Rigler and Elson, (2001) .. Springer, Berlin; Haustein, and Schwille, (2004) .., 531–540). This technique can be applied to determine the dissociation constant of a complex formed by a fluorescence-labelled target and its corresponding RNA aptamer selected v作者: 預兆好 時間: 2025-3-24 15:18 作者: 效果 時間: 2025-3-24 19:25
Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallographyin RNA purification and crystallographic methods have lead to the visualization of an increasing number of unique structures, providing new insights into its function at the atomic level. This article presents general protocols which have streamlined the process of obtaining a homogeneous sample of 作者: 入伍儀式 時間: 2025-3-25 00:25 作者: COWER 時間: 2025-3-25 05:44
Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay a cocktail of two reverse transcriptase (RT) inhibitors and a protease inhibitor. Despite the success of this regimen, there is a continuing need for innovative drug to overcome problems with tolerability and the emergence of viral resistance. The present protocol describes a novel strategy to rapi作者: Amylase 時間: 2025-3-25 09:25 作者: DECRY 時間: 2025-3-25 15:17
Aptamers and Biosensorsbiolayers, the interaction of DNA anti-thrombin aptamer with the protein thrombin was used as a model system. The aptamer was immobilized to a two-dimensional alkanethiol SAM via carboxylamide bonds and to a three-dimensional dextran matrix via streptavidin–biotin interaction. The calculated .. valu作者: Buttress 時間: 2025-3-25 18:50 作者: 長矛 時間: 2025-3-25 21:20 作者: artless 時間: 2025-3-26 03:15
Selection of Peptides Interfering with Protein–Protein Interaction. Consequently, peptides, which are able to specifically interfere with such adventitious interactions, are of high interest for analytical as well as medical purposes. One of the most abundant protein interaction domains is the coiled-coil motif, and thus provides a premier target. Coiled coils, wh作者: Accomplish 時間: 2025-3-26 05:40 作者: albuminuria 時間: 2025-3-26 12:11
Jan Barciszewski,Michael Medgaard,Troels Koch,Jens Kurreck,Volker A. Erdmann writing their PhD dissertation, or (former) colleagues of Niehof, covers the many issues and reflecting her work and interest. The arena of everyday life is what her research and teaching evolved around, as sh978-90-8686-775-2作者: Hay-Fever 時間: 2025-3-26 13:57 作者: 頭腦冷靜 時間: 2025-3-26 19:22 作者: 針葉 時間: 2025-3-26 22:19 作者: Perceive 時間: 2025-3-27 03:29 作者: 下垂 時間: 2025-3-27 05:33
Annette Gaida,Urs B. Hagemann,Dinah Mattay,Christina R?uber,Kristian M. Müller,Katja M. Arndt writing their PhD dissertation, or (former) colleagues of Niehof, covers the many issues and reflecting her work and interest. The arena of everyday life is what her research and teaching evolved around, as sh978-90-8686-775-2作者: 尋找 時間: 2025-3-27 12:51 作者: 浸軟 時間: 2025-3-27 16:42
Shawn K. Piasecki,Bradley Hall,Andrew D. Ellington作者: Mingle 時間: 2025-3-27 18:43 作者: 寒冷 時間: 2025-3-28 00:56
Marguerite Watrin,Eric Dausse,Isabelle Lebars,Bernard Rayner,Anthony Bugaut,Jean-Jacques Toulmé作者: 單獨 時間: 2025-3-28 02:28 作者: 造反,叛亂 時間: 2025-3-28 09:28 作者: Incompetent 時間: 2025-3-28 10:59 作者: 內(nèi)部 時間: 2025-3-28 16:21 作者: parsimony 時間: 2025-3-28 19:26 作者: Deadpan 時間: 2025-3-29 02:12 作者: 人充滿活力 時間: 2025-3-29 04:37 作者: 工作 時間: 2025-3-29 07:34 作者: Range-Of-Motion 時間: 2025-3-29 12:35 作者: 幼稚 時間: 2025-3-29 19:05
Selection of Peptides Interfering with Protein–Protein Interactionthrough all steps of such a process, starting with library options and cloning, detailing suitable selection techniques and ending with purification for further down-stream characterization. Such generated peptides will function as versatile tools to interfere with the natural function of their targ作者: chapel 時間: 2025-3-29 19:52 作者: MIRTH 時間: 2025-3-30 00:05 作者: 你正派 時間: 2025-3-30 05:52 作者: 北極人 時間: 2025-3-30 11:06
Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX)resis (CE) as an enrichment step greatly improves the efficiency of the process. CE–SELEX is capable of isolating high-affinity aptamers in as little as 2–4 rounds of selection, shortening the process time from several weeks to as little as a few days.作者: NEX 時間: 2025-3-30 12:44
Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interactionia systematic evolution of ligands by exponential enrichment (SELEX) (Schürer, et al. (2001) .. ., 47948). Here, an FCS titration experiment is described in detail, where the binding properties of tetramethylrhodamine (TMR) labelled Moenomycin A to its corresponding RNA aptamer were determined (Schürer, et al. (2001) .. ., 47948).作者: orthodox 時間: 2025-3-30 16:33 作者: Hypomania 時間: 2025-3-30 22:35
Aptamers and Biosensorses of about 260 and 267?nM, respectively, were comparable, while the amount of bound analyte varied by a factor of 2, depending on the accessibility of the immobilized aptamer. Differences in the specificity were shown by use of the similar protein elastase.作者: 無目標 時間: 2025-3-31 01:35 作者: 谷物 時間: 2025-3-31 05:11
Nucleic Acid Pool Preparation and Characterization complexity of a nucleic acid pool can sometimes task even an experienced researcher. The following protocol takes the reader through the steps necessary for the preparation of a pool of known complexity.作者: FANG 時間: 2025-3-31 11:03 作者: 一再煩擾 時間: 2025-3-31 16:19 作者: flaggy 時間: 2025-3-31 19:34 作者: 博愛家 時間: 2025-3-31 22:09
Günter MayerProvides an easily accessible reference volume for in vitro selection procedures written by leading researchers in the field.Covers a wide range of in vitro selection protocols targeting different mol作者: 背書 時間: 2025-4-1 03:37
Nucleic Acid and Peptide Aptamers978-1-59745-557-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
