標題: Titlebook: Nucleic Acid Crystallography; Methods and Protocol Eric Ennifar Book 2016 Springer Science+Business Media New York 2016 DNA.Macromolecule P [打印本頁] 作者: postpartum 時間: 2025-3-21 17:36
書目名稱Nucleic Acid Crystallography影響因子(影響力)
書目名稱Nucleic Acid Crystallography影響因子(影響力)學科排名
書目名稱Nucleic Acid Crystallography網絡公開度
書目名稱Nucleic Acid Crystallography網絡公開度學科排名
書目名稱Nucleic Acid Crystallography被引頻次
書目名稱Nucleic Acid Crystallography被引頻次學科排名
書目名稱Nucleic Acid Crystallography年度引用
書目名稱Nucleic Acid Crystallography年度引用學科排名
書目名稱Nucleic Acid Crystallography讀者反饋
書目名稱Nucleic Acid Crystallography讀者反饋學科排名
作者: Irksome 時間: 2025-3-21 23:15 作者: prostate-gland 時間: 2025-3-22 00:32
Eric Westhoften by 16 leading authors with experience of pig research an.The domestic pig is perceived as an animal with intelligence and character and yet, in the industrialized world, the majority of people have had little or no contact with them. Pigs are subject to a wide range of environments from the trop作者: 非秘密 時間: 2025-3-22 08:08 作者: 菊花 時間: 2025-3-22 12:31 作者: LAITY 時間: 2025-3-22 14:54
Alla Peselis,Ang Gao,Alexander Serganovten by 16 leading authors with experience of pig research an.The domestic pig is perceived as an animal with intelligence and character and yet, in the industrialized world, the majority of people have had little or no contact with them. Pigs are subject to a wide range of environments from the trop作者: Inelasticity 時間: 2025-3-22 17:21
Clément Dégut,Alexandre Monod,Franck Brachet,Thibaut Crépin,Carine Tisnémake it possible to produce more and more efficiently. However, the basic requirements for animal well-being are not always taken into account, partly because they need to be defined more precisely in order to be able to implement them. In the meeting on the welfare of pigs, organised under the supe作者: POWER 時間: 2025-3-22 23:28 作者: Cardioversion 時間: 2025-3-23 04:58 作者: 永久 時間: 2025-3-23 08:10
Preparation of Short 5′-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical ny RNAs form specific interactions with proteins often using short nucleotide sequences for protein recognition. Biochemical and structural studies of such RNA–protein complexes demand preparation of short RNAs. Although short RNAs can be synthesized chemically, certain proteins require monophosphat作者: 高射炮 時間: 2025-3-23 12:24
Preparation and Crystallization of Riboswitchesite-sensing segments of mRNAs called riboswitches are unique. Discovered over a decade ago in all three kingdoms of life, riboswitches specifically and directly interact with various metabolites and regulate expression of multiple genes, often associated with metabolism and transport of small molecu作者: 小歌劇 時間: 2025-3-23 16:05 作者: 影響帶來 時間: 2025-3-23 18:55 作者: 萬神殿 時間: 2025-3-23 23:04 作者: Verify 時間: 2025-3-24 04:32 作者: Radiculopathy 時間: 2025-3-24 10:01
Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H:DNA Complexes a considerable portion of the total number of oligo-2′-deoxynucleotide structures determined over the last 30 years. The dodecamer has commonly served as a template to analyze the effects of sequence on DNA conformation, the conformational properties of chemically modified nucleotides, DNA–ligand i作者: Bernstein-test 時間: 2025-3-24 14:05
Isothermal Titration Calorimetry: Assisted Crystallization of RNA–Ligand Complexesble biophysical approaches, isothermal titration calorimetry (ITC) is certainly a method of choice. ITC can be used in a wide range of experimental conditions to monitor in real time the formation of the RNA– or DNA–ligand complex, with the advantage of providing in addition the complete binding pro作者: Mosaic 時間: 2025-3-24 18:19 作者: BATE 時間: 2025-3-24 22:06
Advanced Crystallographic Data Collection Protocols for Experimental Phasingucture determination. Continuous advances at third-generation synchrotron sources have enabled the deployment of rapid data collection protocols that are capable of recording SAD or MAD data sets. However, procedural simplifications driven by the pursuit of high throughput have led to a loss of soph作者: 全部 時間: 2025-3-24 23:57
Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobasesum modifications, researchers can determine novel RNA structures via convenient SAD and MAD phasing. As one of the naturally occurring tRNA modifications, 2-seleno-uridine, which presents almost exclusively at the wobble position of anticodon loop in various bacterial tRNAs (Ching et al., Proc Natl 作者: Flustered 時間: 2025-3-25 05:04 作者: 和諧 時間: 2025-3-25 08:47
Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Datals available for solving protein structures are not available for RNA. We have developed a reliable means to use hexammine cations to address this challenge. The process involves engineering the RNA to introduce a reliable hexammine binding site into the structure, then soaking crystals of these RNA作者: genesis 時間: 2025-3-25 14:18 作者: Leaven 時間: 2025-3-25 16:59
Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?ing continuous helices in the crystal. This arrangement of individual molecules in “infinite” pseudo helical structures in crystal may be the reason why the molecular replacement fails to find a unique position of the search atomic model as the method requires. The Patterson function, calculated as 作者: Defense 時間: 2025-3-25 21:26
1064-3745 Protocols.?presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands..978-1-4939-4641-9978-1-4939-2763-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Intrepid 時間: 2025-3-26 02:26 作者: Mosaic 時間: 2025-3-26 05:47 作者: 起皺紋 時間: 2025-3-26 09:48 作者: 使?jié)M足 時間: 2025-3-26 13:48
Preparation of Short 5′-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical nucleotide for structural and biochemical applications. The chapter describes the principles of construct design, in vitro transcription and RNA purification applied for characterization of a protein that targets the 5′ end of RNA.作者: legitimate 時間: 2025-3-26 18:34
Preparation and Crystallization of Riboswitchesol, it is important to elucidate how riboswitches interact with cognate and discriminate against non-cognate ligands. Here we outline the methodology to synthesize riboswitch RNAs and prepare riboswitch–ligand complexes for crystallographic and biochemical studies. The chapter describes how to desig作者: debacle 時間: 2025-3-26 21:45 作者: EXCEL 時間: 2025-3-27 04:43
Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H:DNA Complexesement does not produce a solution or when crystals of the DNA alone cannot be grown. It is based on the discovery that many dodecamers of the above sequence can be readily co-crystallized with . RNase H, whereby the enzyme is unable to cleave the DNA. Determination of the structure of the complex us作者: EXCEL 時間: 2025-3-27 06:34
Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases Acids Res 40:5171–5179, 2012). In this protocol, we present the efficient chemical synthesis of 2-seleno-uridine triphosphate (.UTP) and the facile transcription and purification of .U-containing RNAs (.U-RNA).作者: 壁畫 時間: 2025-3-27 12:33 作者: chastise 時間: 2025-3-27 16:02 作者: critique 時間: 2025-3-27 20:24
Book 2016s on troubleshooting and avoiding known pitfalls..Authoritative and thorough, .Nucleic Acid Crystallography:?Methods and Protocols.?presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands..作者: Minutes 時間: 2025-3-28 00:38
life and to indicate what the future holds in terms of pig welfare. The text is aimed at researchers and teachers working in veterinary and animal science together with those working in the pig industry and for governmental and non-governmental animal welfare organizations..978-90-481-8024-0978-1-4020-8909-1Series ISSN 1572-7408 作者: Synthesize 時間: 2025-3-28 03:55 作者: 浸軟 時間: 2025-3-28 08:53 作者: 楓樹 時間: 2025-3-28 12:47
life and to indicate what the future holds in terms of pig welfare. The text is aimed at researchers and teachers working in veterinary and animal science together with those working in the pig industry and for governmental and non-governmental animal welfare organizations..978-90-481-8024-0978-1-4020-8909-1Series ISSN 1572-7408 作者: Gum-Disease 時間: 2025-3-28 14:34
Nikita Vasilyev,Alexander Serganov life and to indicate what the future holds in terms of pig welfare. The text is aimed at researchers and teachers working in veterinary and animal science together with those working in the pig industry and for governmental and non-governmental animal welfare organizations..978-90-481-8024-0978-1-4020-8909-1Series ISSN 1572-7408 作者: depreciate 時間: 2025-3-28 21:47 作者: 付出 時間: 2025-3-28 23:26
Clément Dégut,Alexandre Monod,Franck Brachet,Thibaut Crépin,Carine Tisnéuture research. The meeting was a fruitful first step in bringing together different opinions in order to arrive at a universally acceptable common solution for modern pig production. W. Sybesma SESSION I BEHAVIOURAL AND PHYSIOLOGICAL NEEDS OF PIGS IN GENERAL I. BASIC NEEDS Chairman: J.P. Signoret BEHAVIOURAL978-94-011-9574-4作者: Forehead-Lift 時間: 2025-3-29 06:22 作者: 一致性 時間: 2025-3-29 10:34 作者: 嘮叨 時間: 2025-3-29 13:36
Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction DataAD or MAD phasing. In many cases, suitable derivatives can be obtained by soaking the hexammine into RNA crystals that have not been engineered. Considerations for using this method and example protocols are presented.作者: APRON 時間: 2025-3-29 16:23 作者: palliate 時間: 2025-3-29 22:12
Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA by changing the percentage and reticulation of the gel material. In this protocol, we focus on the preparation of milligram-scale amounts of ~200 nucleotides (nt) RNA molecules that were used in subsequent crystallization experiments.作者: Tracheotomy 時間: 2025-3-30 03:04 作者: Deadpan 時間: 2025-3-30 07:47
Isothermal Titration Calorimetry: Assisted Crystallization of RNA–Ligand Complexesfile of the interaction. Following the ITC experiment, the complex is ready to be concentrated for crystallization trials. This chapter describes a detailed experimental protocol for using ITC as a tool for monitoring RNA/small molecule binding, followed by co-crystallization.作者: Resistance 時間: 2025-3-30 08:50
Advanced Crystallographic Data Collection Protocols for Experimental Phasings well as from the instrument. This chapter also emphasizes data processing for “on-the-fly” decision-making during data collection, a critical process when data quality depends directly on information gathered while at the synchrotron.作者: Magnitude 時間: 2025-3-30 15:03 作者: 先兆 時間: 2025-3-30 19:34 作者: 偽造者 時間: 2025-3-30 23:20 作者: RAG 時間: 2025-3-31 04:53
Eric EnnifarIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 確保 時間: 2025-3-31 08:55
978-1-4939-4641-9Springer Science+Business Media New York 2016
