作者: BRAND 時(shí)間: 2025-3-21 22:17 作者: Pedagogy 時(shí)間: 2025-3-22 02:07 作者: BET 時(shí)間: 2025-3-22 05:26 作者: 沉默 時(shí)間: 2025-3-22 09:36 作者: Barrister 時(shí)間: 2025-3-22 14:04 作者: 人充滿活力 時(shí)間: 2025-3-22 18:34 作者: 橫截,橫斷 時(shí)間: 2025-3-23 00:45
Olga ?strup,Hanne S. Pedersen,Hanne M. Holm,Poul Hyttel作者: Irremediable 時(shí)間: 2025-3-23 03:42 作者: 強(qiáng)所 時(shí)間: 2025-3-23 06:58
Nuclear Transfer in Rabbit, of the mammalian species considered as difficult to clone. This chapter presents the technical protocol used nowadays to achieve the goal to obtain cloned embryos able to develop to term using fresh somatic cumulus cells.作者: 夾克怕包裹 時(shí)間: 2025-3-23 09:47
Visualization of Epigenetic Modifications in Preimplantation Embryos,lize histone modifications in living embryos, a Fab-based live endogenous modification labeling (FabLEM) technique has been developed. Here we describe the methods required for FabLEM experiments, including Fab preparation from IgG, its conjugation with a fluorescent dye, loading into cultured cells or mouse embryos, and imaging.作者: chondromalacia 時(shí)間: 2025-3-23 16:49 作者: kyphoplasty 時(shí)間: 2025-3-23 18:16 作者: 隱語 時(shí)間: 2025-3-23 23:05
978-1-4939-5441-4Springer Science+Business Media New York 2015作者: 發(fā)芽 時(shí)間: 2025-3-24 04:17
Nuclear Reprogramming978-1-4939-1594-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Outshine 時(shí)間: 2025-3-24 06:58 作者: 敵意 時(shí)間: 2025-3-24 14:43 作者: 流出 時(shí)間: 2025-3-24 18:45
Nathalie Beaujean,Hélène Jammes,Alice JouneauIncludes fully updated, cutting-edge methods and protocols for nuclear reprogramming.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from t作者: trigger 時(shí)間: 2025-3-24 19:17
Nuclear Transfer in the Mouse,em (ntES) cells from living or frozen animals. The Piezo-driven direct microinjection NT method has proved to be a valid technique to clone mice and other species. In addition, this method has been broadly used as a versatile tool for many fields of mouse micromanipulation. This chapter describes th作者: Processes 時(shí)間: 2025-3-25 02:30
Nuclear Transfer in Rabbit, of the mammalian species considered as difficult to clone. This chapter presents the technical protocol used nowadays to achieve the goal to obtain cloned embryos able to develop to term using fresh somatic cumulus cells.作者: Cocker 時(shí)間: 2025-3-25 07:13
Nuclear Transfer in Ruminants,t animals (Willadsen, Nature 320(6057):63–65, 1986; Prather et al., Biol Reprod 37(4):859–866, 1987; Wilmut et al., Nature 385(6619):810–813, 1997). Since then, cloning has provided us with a vast amount of knowledge and information on the reprogramming ability of somatic cells to different cell typ作者: Inveterate 時(shí)間: 2025-3-25 08:01
Nuclear Transfer and Transgenesis in the Pig,and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, we introduce our routine workflow for the production of genetically engineered pigs, especially focused o作者: Clinch 時(shí)間: 2025-3-25 12:28
,Embryonic Stem Cell–Somatic Cell Fusion and Postfusion Enucleation,ties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes can be sustained following removal of the dominant ES nucleus. In this chapter, methods are described for the cell fu作者: beta-carotene 時(shí)間: 2025-3-25 16:25
Analysis of Nuclear Reprogramming Following Nuclear Transfer to , Oocyte, nuclear transfer no cell division occurs and no new cell types are generated. However, the transplanted nuclei undergo extensive transcriptional reprogramming..Here, it is first explained how to carry out transplantation of multiple mammalian cell nuclei to . oocytes. It is then described how to pe作者: Panther 時(shí)間: 2025-3-25 22:54
Assessing the Quality of Donor Cells: Karyotyping Methods,nced by the biological material used (oocyte and donor cell quality). Hence, it is crucial to check the normality of the donor cell’s karyotype. Numerical and structural chromosome abnormalities are detected by cytogenetic analysis at minimum using G-banding to identify the chromosomes. Here, we des作者: 羅盤 時(shí)間: 2025-3-26 00:21 作者: gregarious 時(shí)間: 2025-3-26 05:11 作者: 臥虎藏龍 時(shí)間: 2025-3-26 09:32 作者: dysphagia 時(shí)間: 2025-3-26 13:19
Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer,bryos consider only embryos at predefined key stages (e.g., morula or blastocyst), that is, after the bulk of reprogramming has taken place. These retrospective approaches are of limited use to elucidate mechanisms of reprogramming and to predict developmental success. Observing cloned embryo develo作者: Brain-Imaging 時(shí)間: 2025-3-26 20:37
Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming,rence of the reprogramming. This phenomenon is most pronounced when differentiated cells are reprogrammed to totipotency when they are submitted to cloning by somatic cell nuclear transfer. However, when cells are reprogrammed by less fundamental means, as for example treatment by Xenopus extract or作者: glacial 時(shí)間: 2025-3-26 23:57 作者: 勤勉 時(shí)間: 2025-3-27 03:23 作者: arousal 時(shí)間: 2025-3-27 07:59
Studying Bovine Early Embryo Transcriptome by Microarray, genes even if the sample of interest contains small quantities of genetic material. Here we describe the protocols developed by the EmbryoGENE Network to study the bovine transcriptome in early embryo using a microarray experimental design.作者: Debility 時(shí)間: 2025-3-27 11:13 作者: LITHE 時(shí)間: 2025-3-27 16:10 作者: 不自然 時(shí)間: 2025-3-27 18:05
Assessing Reprogramming by Chimera Formation and Tetraploid Complementation,n more, tetraploid complementation. Whether iPS cells in general are functionally equivalent to normal ESCs is difficult to establish. Here, we present the detailed procedure for chimera formation and tetraploid complementation, the most stringent criterion, to assessing pluripotency.作者: THE 時(shí)間: 2025-3-27 22:29
Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology,thods of probe labeling and hybridization detection are available nowadays. Meanwhile the technique was adapted to be used on many different species and has evolved from a manual to a larger scale and automated procedure. Standardized automated protocols improve the chance to compare different exper作者: 容易生皺紋 時(shí)間: 2025-3-28 05:35
Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer,tone H2b-GFP expressing mouse embryos, to record cell divisions up to the blastocyst stage. This protocol, which can be adapted to observe other reporters such as Oct4-GFP or Nanog-GFP, allowed us to quantitatively analyze cleavage kinetics of cloned embryos.作者: 神圣不可 時(shí)間: 2025-3-28 09:52
Nuclear Transfer in the Mouse,le manipulation procedure. This protocol describes the isolation and collection of oocytes, treatment of donor cells, visualization of spindle-chromosomal complex, direct injection and enucleation, activation of reconstructed embryos and their in vitro culture and transfer into pseudopregnant mice.作者: 強(qiáng)所 時(shí)間: 2025-3-28 13:02
Nuclear Transfer in Ruminants,t cases the micromanipulation procedures remain the same. However, differences between species require different technical considerations. In this chapter, we describe in detail somatic cell nuclear transfer which is the foremost method for cloning ruminants with specific reference to sheep and cattle.作者: Spinal-Tap 時(shí)間: 2025-3-28 16:17
Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transracticed technique. Thus, it is very important to find simple and reproducible methods for improving the success rate of SCNT. In this chapter, we will review our recent protocols, which seem to be the simplest and most reliable method to date to improve development of SCNT embryos.作者: 爆米花 時(shí)間: 2025-3-28 21:44
,Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos,io output, suitable for as few as 100 cells. This chapter provides a detailed protocol for μChIP from early mammalian embryos, also suitable for any sample of limited numbers of cells. Minor modifications of this optimized high signal to noise ChIP protocol make it a reliable tool for the use with any cell number (100–10.).作者: 使高興 時(shí)間: 2025-3-29 00:46
1064-3745 results.Contains key notes and implementation advice from t.Nuclear Reprogramming: Methods and Protocols, Second Edition. includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embr作者: –scent 時(shí)間: 2025-3-29 06:22
Nuclear Transfer and Transgenesis in the Pig,available for this species. In this chapter, we introduce our routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, SCNT using in vitro matured oocytes, and laparoscopic embryo transfer.作者: Androgen 時(shí)間: 2025-3-29 11:02
Analysis of Nuclear Reprogramming Following Nuclear Transfer to , Oocyte,ogramming..Here, it is first explained how to carry out transplantation of multiple mammalian cell nuclei to . oocytes. It is then described how to perform RT-qPCR, Western Blot, Chromatin Immunoprecipitation, and live imaging analysis to monitor transcriptional reprogramming of the nuclei transplanted to oocytes.作者: labyrinth 時(shí)間: 2025-3-29 15:03
Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining, to look at cell position and to analyze and count the proportions between the different cell types. Thus after any kind of embryo manipulation such as nuclear transfer (NT), the analysis of the three cell lineages by immunofluorescence will provide criteria for good or poor development.作者: defile 時(shí)間: 2025-3-29 18:42 作者: 龍蝦 時(shí)間: 2025-3-29 23:44
Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level,e thus developed a very efficient protocol for methylation studies on individual oocytes or cleavage-stage embryos. All the different steps were optimized, from DNA extraction, to limit DNA degradation and give a high success rate of bisulfite converted DNA, to amplification of the bisulfite modified DNA.作者: 兩棲動(dòng)物 時(shí)間: 2025-3-30 01:28
,Embryonic Stem Cell–Somatic Cell Fusion and Postfusion Enucleation,sion of mouse tetraploid ES cells with somatic cells and enrichment of the resulting heterokaryons. We next describe the conditions for the differential removal of the ES cell nucleus, allowing for the recovery of somatic cells.作者: cavity 時(shí)間: 2025-3-30 05:36
Assessing the Quality of Donor Cells: Karyotyping Methods,cribe the classical protocols that are needed to perform complete cytogenetic analyses, i.e., G-banding to identify chromosome aberrations, followed by Fluorescent In Situ Hybridization (FISH) of specific probes for a more sensitive detection and precise identification of the rearrangement.作者: 領(lǐng)導(dǎo)權(quán) 時(shí)間: 2025-3-30 10:55 作者: Incumbent 時(shí)間: 2025-3-30 14:29
Book 2015Latest editionalso several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflec作者: Fecundity 時(shí)間: 2025-3-30 18:13 作者: laceration 時(shí)間: 2025-3-30 23:09 作者: Eulogy 時(shí)間: 2025-3-31 03:20
The State and the Regulation of Marriage: Sefwi Wiawso (Ghana), 1900–40rs earlier. Between about 1925 and his death in 1932, . Kwame Tano I and the Sefwi Wiawso State Council passed several amendments to customary law relating to marriage. Further amendments were made in the subsequent decade.作者: Magnificent 時(shí)間: 2025-3-31 07:16
https://doi.org/10.1007/978-3-663-11088-0sibility of not only the current information but also previously hidden information. In this chapter, we first introduce the structure of RNN and then mainly focus on two important RNN types: long short-term memory (LSTM) and gated recurrent unit (GRU). Finally, we use case studies to deepen the understanding of RNN and LSTM.作者: 證明無罪 時(shí)間: 2025-3-31 12:06
Expression of the Local GPS Solution in the Regional Reference Frame ETRF2000,transformed into ETRF2000. The minimum constraints approach has been applied preserving original characteristic of the local GPS solution, and in the same time, the local GPS solution was expressed in the regional reference frame through the mathematical definition of the ETRS89.作者: choleretic 時(shí)間: 2025-3-31 14:29 作者: BLANK 時(shí)間: 2025-3-31 20:19
What We Think We Know About Metal Thievesdivergierende soziologische Theorieperspektiven konstruktiv aufeinander zu beziehen. Ziel des Theorienvergleichs ist dabei nicht l?nger die Elimination konkurrierender Theorieans?tze, sondem das Ausloten von Identit?ten und Differenzen, die Lokalisierung von überg?ngen und abgebrochenen Verbindungen作者: 使熄滅 時(shí)間: 2025-3-31 22:18
Franklin E. Roachnction of wild animals led to the enactment of the first Indian law in 1887 followed by another in 1912 and many more legislations thereafter. However, the initial conservation laws were primarily concerned with regulating hunting privileges but did not completely prohibit trade in wildlife products作者: 機(jī)警 時(shí)間: 2025-4-1 03:26
Adrenal Incidentalomaslly limited (2–5). The other important question is whether early diagnosis and treatment is more effective in presymptomatic patients and whether the beneficial effects justify the costs. Studies on this issue are subject to lead-time bias, which refers to fictitious extension of life due to an anti作者: OPINE 時(shí)間: 2025-4-1 06:34 作者: uveitis 時(shí)間: 2025-4-1 13:49
Jan TachezyPresents the most recent knowledge of these "unusual sons of the mitochondrial family", including the theory of their evolution.Includes supplementary material: 作者: Enteropathic 時(shí)間: 2025-4-1 15:52 作者: 針葉 時(shí)間: 2025-4-1 19:05
