作者: 格言 時(shí)間: 2025-3-21 22:12 作者: 現(xiàn)代 時(shí)間: 2025-3-22 02:47 作者: Metastasis 時(shí)間: 2025-3-22 08:00 作者: 容易懂得 時(shí)間: 2025-3-22 10:44 作者: Engaged 時(shí)間: 2025-3-22 16:00
Ricardo Raúl Cevallos,Md Emon Hossain,Ruowen Zhang,Kejin Hut and proceeded to pass laws. The present constitution, which came into force on May 1, 1934. begins with the statement :— “In the Name of God the Almighty from Whom all right proceeds, the Austrian people receives this Constitution for its Christian, German, Federal State on a corporative foundatio作者: FIR 時(shí)間: 2025-3-22 18:23
Erik McIntire,Kimberly Leonhard,Seth Taapken,Anna Lisa Larsong out a precarious pastoral existence by the breeding of camels, sheep and goats. Bounded on the north by Iraq and Transjordan (Palestine), it is enclosed on the other three sides by the sea—the Red Sea on the west, the Arabian Sea on the south and the Persian Gulf on the east. The land-surface of t作者: Nonporous 時(shí)間: 2025-3-22 23:47 作者: 重畫只能放棄 時(shí)間: 2025-3-23 03:28 作者: 并入 時(shí)間: 2025-3-23 07:11
Pig Cloning Using Somatic Cell Nuclear Transfer,etically modified pigs have been produced by somatic cell nuclear transfer using genetically modified porcine fetal fibroblasts. The method of preparing genetically modified pigs is critical for establishing pig models for human diseases, and for generating donor animals for future xenotransplantati作者: 樂器演奏者 時(shí)間: 2025-3-23 13:00
Cloning of Monkeys by Somatic Cell Nuclear Transfer,omedical research. We have recently cloned monkeys by optimization of the SCNT protocols and inclusion of the epigenetic modulator. Here, we describe the protocol for generation of cloned monkeys by somatic cell nuclear transfer.作者: JAUNT 時(shí)間: 2025-3-23 17:29
Production of Cardiomyocyte-Like Cells by Fibroblast Reprogramming with Defined Factors,s into another type of differentiated cells, such as direct reprogramming of fibroblasts into cardiomyocytes, without passage through an undifferentiated pluripotent stage. Discovery of direct cardiac reprogramming offers a promising therapeutic strategy to prevent/attenuate cardiac fibrotic remodel作者: Initial 時(shí)間: 2025-3-23 19:23 作者: otic-capsule 時(shí)間: 2025-3-23 23:13
Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene E This phenomenon can be mediated in the starting somatic cells by the introduction of lineage-specific master transcription factors or by pluripotency factors routinely used in iPS cell generation. In the latter process known as .luripotency factor-mediated .irect .eprogramming (PDR), the pluripoten作者: patriot 時(shí)間: 2025-3-24 02:52
Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts,ing the fibroblast identity and evoking a pan-neuronal state. In contrast to induced pluripotent stem cell-derived neurons, miRNA-induced neurons (miNs) retain the biological age of the starting fibroblasts through direct fate conversion and thus provide a human neuron-based platform to study cellul作者: 支形吊燈 時(shí)間: 2025-3-24 07:17 作者: Fillet,Filet 時(shí)間: 2025-3-24 14:44 作者: Panacea 時(shí)間: 2025-3-24 17:26
Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood MononuT4, SOX2, KLF4, and c-MYC. Sustained expression of the transgenes during reprogramming is crucial for the successful derivation of iPSCs. Integrating retroviruses have been used to achieve the required prolonged expression; however, issues of undesirable transgene expression in the iPSC-derived cell作者: ANTI 時(shí)間: 2025-3-24 22:49
Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling,ells (e.g., fibroblasts), without the need for destruction of human embryos. This provides an unprecedented opportunity where patient-specific iPSCs can be subsequently differentiated to many cell types, e.g., cardiac cells and neurons, so that we can use these iPSC-derived cells to study patient-sp作者: prosthesis 時(shí)間: 2025-3-25 00:50 作者: 躺下殘殺 時(shí)間: 2025-3-25 04:55
Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators,ctors, human fibroblasts can be reprogrammed into induced pluripotent stem cells (iPSCs). Here, we describe a method for the derivation of iPSCs from human fibroblasts using episomal plasmids encoding CRISPRa components. This chapter also provides procedure to assemble guide RNA cassettes and genera作者: anagen 時(shí)間: 2025-3-25 09:31 作者: 不公開 時(shí)間: 2025-3-25 12:47
Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance, proliferation of human pluripotent stem cells (hPSCs). Here, we describe the necessary steps to derive, expand, harvest, inactivate, plate, and use MEFs as feeders for iPSC generation and maintenance.作者: conifer 時(shí)間: 2025-3-25 16:13
Evaluating Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotencyf ethical restrictions. One common and initial approach to prove the pluripotency of an established human iPSC line is to demonstrate expression of a set of established surface and intracellular pluripotency markers. This chapter provides procedures of immunocytochemistry of the established HiPSC li作者: acquisition 時(shí)間: 2025-3-25 21:14
G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures,hat regular testing of genetic integrity be conducted. G-banded karyotyping is a widely used genetic assay that is capable of detecting chromosomal abnormalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for k作者: 煩擾 時(shí)間: 2025-3-26 01:05
Become Competent in Generating RNA-Seq Heat Maps in One Day for Novices Without Prior R Experience,gical scientists are afraid to prepare heat maps independently because R is a programming platform. Here, using RNA-seq data for 16 differentially expressed genes in . pathway between embryonic stem cells and fibroblasts,?I share a tutorial for novices without any prior R experience to master the sk作者: Morphine 時(shí)間: 2025-3-26 04:18 作者: COLON 時(shí)間: 2025-3-26 08:29
https://doi.org/10.1007/978-1-0716-1084-8iPSC reprogramming; cardiac progenitors; fibroblasts; episomal vectors; retroviral vectors作者: 預(yù)感 時(shí)間: 2025-3-26 16:29
978-1-0716-1086-2Springer Science+Business Media, LLC, part of Springer Nature 2021作者: 攤位 時(shí)間: 2025-3-26 17:20
Nuclear Reprogramming978-1-0716-1084-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: CHANT 時(shí)間: 2025-3-26 23:14
Kejin HuIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: BOOST 時(shí)間: 2025-3-27 04:11
Cloning of Monkeys by Somatic Cell Nuclear Transfer,omedical research. We have recently cloned monkeys by optimization of the SCNT protocols and inclusion of the epigenetic modulator. Here, we describe the protocol for generation of cloned monkeys by somatic cell nuclear transfer.作者: 飛行員 時(shí)間: 2025-3-27 07:47
Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance, proliferation of human pluripotent stem cells (hPSCs). Here, we describe the necessary steps to derive, expand, harvest, inactivate, plate, and use MEFs as feeders for iPSC generation and maintenance.作者: MOTIF 時(shí)間: 2025-3-27 10:58 作者: 有抱負(fù)者 時(shí)間: 2025-3-27 13:57 作者: ENACT 時(shí)間: 2025-3-27 21:19 作者: Libido 時(shí)間: 2025-3-27 22:49
Book 2021ic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful .Method作者: Tartar 時(shí)間: 2025-3-28 05:45
Pig Cloning Using Somatic Cell Nuclear Transfer,ng genetically modified pigs is critical for establishing pig models for human diseases, and for generating donor animals for future xenotransplantation. This chapter describes detailed procedures for generating cloned pigs using fetal fibroblasts as nuclear donors.作者: Semblance 時(shí)間: 2025-3-28 10:10
Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral V the chemically defined medium. Fibroblasts are the most common starting cells for iPSC generation. Here, we provide a detailed protocol for iPSC generation from human fibroblasts using the GFP-expressing lentiviral vectors in the chemically defined medium.作者: 惰性氣體 時(shí)間: 2025-3-28 10:50 作者: CREST 時(shí)間: 2025-3-28 18:04
Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA, have limitations. Here, I describe a method to reprogram human fibroblasts using a cocktail of mRNAs without any base modification that increases reprogramming efficiency, reduces the RNA-associated toxicity, and yields iPSCs ready for expansion and characterization in as short as 10–14?days.作者: PANT 時(shí)間: 2025-3-28 21:42
Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators,human fibroblasts using episomal plasmids encoding CRISPRa components. This chapter also provides procedure to assemble guide RNA cassettes and generation of multiplexed guide plasmids for readers who want to design their own guide RNAs.作者: Intrepid 時(shí)間: 2025-3-29 00:31
G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures,normalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for karyotyping, including sample preparation and analysis, as well as the interpretation of hPSC karyotype results.作者: cinder 時(shí)間: 2025-3-29 05:29 作者: 表被動 時(shí)間: 2025-3-29 09:39 作者: 衰老 時(shí)間: 2025-3-29 13:56
Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts,g disorders that affect specific neuronal subtypes. Here, we describe the miR-9/9.-124-based neuronal reprogramming protocols for the generation of several disease-relevant neuronal subtypes: striatal medium spiny neurons, cortical neurons, and spinal cord motor neurons.作者: backdrop 時(shí)間: 2025-3-29 16:13 作者: Efflorescent 時(shí)間: 2025-3-29 22:49 作者: 羽飾 時(shí)間: 2025-3-30 00:46
Kejin Huor special purposes. There are a President, two Vice-Presidents and a ministry, divided into six departments—of Foreign Relations and Worship; Finance; Government and Public Works; Justice and Industry; War and Colonisation; and Education and Agriculture.作者: 先驅(qū) 時(shí)間: 2025-3-30 04:52
his mountainous district and the similar district of the Yemen, Arabia is a barren country consisting of vast tracts of steppe-desert, sand-waste and mountainous wilderness. It is a country of insignificant rainfall (the Yemen and Oman excepted) ; here and there, scattered oases, or oasis-groups, are formed.作者: 倔強(qiáng)不能 時(shí)間: 2025-3-30 08:26
ophers, and social historians. The contributors examine utopias hitherto never or rarely reviewed in the English language, making this book of interest to students and scholars in economics, political science a978-1-4614-2776-6978-1-4419-7500-3Series ISSN 1572-1744 Series E-ISSN 2197-5892 作者: 陳列 時(shí)間: 2025-3-30 14:41 作者: 現(xiàn)暈光 時(shí)間: 2025-3-30 19:25 作者: FLOUR 時(shí)間: 2025-3-31 00:39 作者: 原始 時(shí)間: 2025-3-31 03:04
Ricardo Raúl Cevallos,Md Emon Hossain,Ruowen Zhang,Kejin Hu the Chancellor’s countersignature. The Cultural Council will consist of 30 to 40 representatives of the Churches and religious communities, of education, learning, and the arts, who have reached the age of 26.作者: 盤旋 時(shí)間: 2025-3-31 08:18 作者: CODA 時(shí)間: 2025-3-31 12:48 作者: grovel 時(shí)間: 2025-3-31 15:37
Production of Cardiomyocyte-Like Cells by Fibroblast Reprogramming with Defined Factors,al cardiac, tail-tip, and adult cardiac fibroblasts), retroviral preparation of reprogramming factors, and iCM generation by fibroblast reprogramming via retroviral transduction of Gata4, Mef2c, and Tbx5. A detailed written protocol will help many other laboratories, inexperienced in this area, to u作者: 確定的事 時(shí)間: 2025-3-31 20:48
Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene Ethe episomal vectors that incorporate a suicide gene . (encoding cytosine deaminase) that allows rapid and efficient generation of a homogenous population of transgene-free human-induced neural progenitor cells (hiNPCs). The resulting NESTIN./PAX6./CDH2. hiNPCs can be expanded and cryopreserved and
