作者: 使厭惡 時(shí)間: 2025-3-21 22:50
Isolation of Stromal Stem Cells From Human Adipose Tissue,es procedures for isolating millions of highly purified stromal stem cells from human adipose tissue and methods of establishing polyclonal and monoclonal cultures of adipose tissue-derived stem cells.作者: 百科全書 時(shí)間: 2025-3-22 03:49
Polyethylene Glycol-Mediated Cell Fusion, of cells. Using this method, we have successfully introduced mammalian minichromosomes into a variety of vertebrate cells. The technique described here can be adapted for uses in other cell fusion involved research. This protocol, in principle, provides guidelines for further development of PEG mediated cell fusion technology.作者: Ossification 時(shí)間: 2025-3-22 05:54 作者: Ossification 時(shí)間: 2025-3-22 09:03
Pluripotency: ,,soderm and endoderm, respectively. In the future, differentiated cells may be also generated from human ES cells by cultivation of early embryos or from reprogrammed cells derived by nuclear transfer. Such cells could represent potential sources for tissue repair of serious human diseases.作者: 兵團(tuán) 時(shí)間: 2025-3-22 14:57
Chromatin Immunoprecipitation Assay for Mammalian Tissues,) allows researchers to determine the status of both histone modifications and the nuclear effector proteins located at gene of interest. ChIP, if applied globally, can also reveal how chromatin structures are dynamically changed when cells respond to certain stimuli. In this chapter, we describe this powerful technique in detail.作者: helper-T-cells 時(shí)間: 2025-3-22 17:24
Nuclear Transplantation in ,,er, the methods used to transplant living nuclei into enucleated eggs of . are described. A method also is described for transplanting multiple somatic cell nuclei into nonenucleated oocytes, a procedure that achieves reprogramming of gene expression in the absence of cell division.作者: 議程 時(shí)間: 2025-3-22 21:35 作者: 節(jié)約 時(shí)間: 2025-3-23 02:44 作者: 遺傳學(xué) 時(shí)間: 2025-3-23 09:11
Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos,Immunofluorescence using antibodies against 5-methylcytosine is an invaluable method for the large-scale screening of genome-wide methylation. This method has a high degree of reproducibility, essential for the analysis of small numbers of valuable samples, providing information on methylation profiles of individual cells and embryos.作者: 巨大沒(méi)有 時(shí)間: 2025-3-23 11:04 作者: 雪白 時(shí)間: 2025-3-23 16:10
1064-3745 ransfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telome作者: propose 時(shí)間: 2025-3-23 21:35 作者: effrontery 時(shí)間: 2025-3-23 23:37
Steve PellsIncludes supplementary material: 作者: 過(guò)于光澤 時(shí)間: 2025-3-24 03:56 作者: 嘲笑 時(shí)間: 2025-3-24 07:40
Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis,e of the zygote should be brought about in the early cleavage stages. DNA methylation is a typical epigenetic mark modified during pre-implantation development. Here, we describe how to analyze DNA methylation profiles in early-stage embryos.作者: 河潭 時(shí)間: 2025-3-24 13:21 作者: 偶然 時(shí)間: 2025-3-24 15:08
https://doi.org/10.1385/1597450057Activation; DNA; Ethylen; Monoclonal Antibodies; PCR; Telomere; Vivo; transcription作者: 新字 時(shí)間: 2025-3-24 21:10
Stephen Sullivan,Martin Waterfall,Ed J. Gallagher,Jim McWhir,Steve Pellsof seventy years (1952-2022), Lundgren challenges the idea that many of the recent controversies concerning religious diversity are new. She argues that many of the discussions held today regarding the accommod978-3-031-42157-0978-3-031-42155-6Series ISSN 2214-5281 Series E-ISSN 2214-529X 作者: 抱怨 時(shí)間: 2025-3-24 23:28
Rong Wu,Anna V. Terry,David M. Gilbertophers, and social historians. The contributors examine utopias hitherto never or rarely reviewed in the English language, making this book of interest to students and scholars in economics, political science a978-1-4614-2776-6978-1-4419-7500-3Series ISSN 1572-1744 Series E-ISSN 2197-5892 作者: terazosin 時(shí)間: 2025-3-25 07:25
Jacques-E. Fléchonophers, and social historians. The contributors examine utopias hitherto never or rarely reviewed in the English language, making this book of interest to students and scholars in economics, political science a978-1-4614-2776-6978-1-4419-7500-3Series ISSN 1572-1744 Series E-ISSN 2197-5892 作者: Encumber 時(shí)間: 2025-3-25 09:19 作者: Default 時(shí)間: 2025-3-25 14:38 作者: 搜集 時(shí)間: 2025-3-25 17:39 作者: 金盤是高原 時(shí)間: 2025-3-25 22:28
Anne-Mari H?kelien,Kristine G. Gaustad,Philippe CollasECD states.These themes are explored in detail through contributions by economists, philosophers, and social historians. The contributors examine utopias hitherto never or rarely reviewed in the English language, making this book of interest to students and scholars in economics, political science a作者: 后來(lái) 時(shí)間: 2025-3-26 03:01
Megan Munsie,Peter Mountford,Jennifer NicholsECD states.These themes are explored in detail through contributions by economists, philosophers, and social historians. The contributors examine utopias hitherto never or rarely reviewed in the English language, making this book of interest to students and scholars in economics, political science a作者: BRIEF 時(shí)間: 2025-3-26 06:46 作者: apiary 時(shí)間: 2025-3-26 10:53 作者: 無(wú)思維能力 時(shí)間: 2025-3-26 16:05 作者: liaison 時(shí)間: 2025-3-26 17:47
Bruce A. Fenderson,Maria P. De Miguel,April D. Pyle,Peter J. DonovanECD states.These themes are explored in detail through contributions by economists, philosophers, and social historians. The contributors examine utopias hitherto never or rarely reviewed in the English language, making this book of interest to students and scholars in economics, political science a作者: 誘使 時(shí)間: 2025-3-26 23:46 作者: Assault 時(shí)間: 2025-3-27 01:23 作者: PANT 時(shí)間: 2025-3-27 07:11 作者: 來(lái)就得意 時(shí)間: 2025-3-27 11:25
Nuclear Reprogramming by Cell Fusion,m switching is mediated by .-acting factors. Other possible uses of this cell fusion protocol in stem cell biology and transplantation of genetically engineered cells for tissue regeneration are briefly discussed.作者: 書法 時(shí)間: 2025-3-27 14:42
Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells,clei. Interestingly, nuclear reprogramming for conferring pluripotency on somatic nuclei also occurs via cell fusion between pluripotential stem cells and somatic cells. Furthermore, it has been shown that spontaneous cell fusion contributes to generating the intrinsic plasticity of tissue stem cell作者: Rinne-Test 時(shí)間: 2025-3-27 20:51 作者: Notorious 時(shí)間: 2025-3-28 01:58
Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones,hromosome capping. Telomere-mediated signaling of cellular senescence has been established for many somatic cell types in vitro, except for germ cells, cancer lines, and regenerative tissues in which telomere length is maintained primarily by the ribonucleoprotein telomerase, a reverse transcriptase作者: 完全 時(shí)間: 2025-3-28 04:18
Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens,res; (2) examine the spatial distribution of stem cells and differentiated derivatives in monolayer cultures and embryoid bodies; and (3) isolate populations of differentiated and undifferentiated cells by immunomagnetic or fluorescence-activated cell sorting for cellular and molecular analyses.作者: 繁重 時(shí)間: 2025-3-28 07:28 作者: 吞沒(méi) 時(shí)間: 2025-3-28 12:59
Methylation-Sensitive Polymerase Chain Reaction,um bromide-stained agarose gel is proportional to the amount of methylated DNA in each embryo. By comparing quadruplicate values obtained for each embryo against a standard curve, we are able to ensure the validity of our results for each individual embryo. Compared with bisulphite sequencing method作者: 泰然自若 時(shí)間: 2025-3-28 17:05
1064-3745 eosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.978-1-61737-530-9978-1-59745-005-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: shrill 時(shí)間: 2025-3-28 22:41
Nuclear Transplantation in ,,ifferentiating cells, and larval-differentiated cells. Transplanted nuclei are reprogrammed to entirely new patterns of gene expression. In this chapter, the methods used to transplant living nuclei into enucleated eggs of . are described. A method also is described for transplanting multiple somati作者: Injunction 時(shí)間: 2025-3-29 02:21 作者: ITCH 時(shí)間: 2025-3-29 03:15
Protocols for Nuclear Transfer in Mice,tulate the developmental program. This chapter summarizes the method used since 1998 for mouse cloning, which differs from that for large animal cloning. A Piezo-drill micromanipulator allows direct injection of nuclei into enucleated mice oocytes instead of the electrofusion method used for introdu作者: OTTER 時(shí)間: 2025-3-29 07:17
Isolation of Stromal Stem Cells From Human Adipose Tissue,l. Stromal stem cells offer a potentially large therapeutic potential in the field of regenerative medicine. Adipose tissue contains a large number of stromal stem cells, is relatively easy to obtain in large quantities, and thus constitutes a very convenient source of stromal stem cells. Importantl作者: 農(nóng)學(xué) 時(shí)間: 2025-3-29 14:24
Nuclear Reprogramming by Cell Fusion,e expression was pioneered by Harris and Ringertz more than three decades ago. The ability to make heterokaryons with cells from different species or genetic strains is especially useful because genetic differences in gene products allow the origin of .-acting regulatory factors to be determined. He作者: cunning 時(shí)間: 2025-3-29 16:59 作者: aplomb 時(shí)間: 2025-3-29 23:33
Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells,cells between mouse embryonic stem cells and somatic cells, which is a type of nonchemically induced and nonvirus-mediated cell fusion, is introduced here as a highly effective, reproducible, and biomedically safe in vitro system. Under optimized electrofusion conditions, cells are aligned and form 作者: 聯(lián)合 時(shí)間: 2025-3-30 03:35 作者: 錯(cuò) 時(shí)間: 2025-3-30 04:55 作者: 警告 時(shí)間: 2025-3-30 09:03
Transgenic Systems in Nuclear Reprogramming, fusion hybrids to providing a means to display levels and distribution of specific gene products after nuclear transfer. Here, the method of piezo-assisted direct injection, which has been used previously to generate blastocysts and subsequently embryonic stem cell lines by transfer of nuclei from 作者: 東西 時(shí)間: 2025-3-30 15:32 作者: 哭得清醒了 時(shí)間: 2025-3-30 16:40
Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones,es and postnatal abnormalities observed after nuclear transplantation in domestic animal clones. One chromosome structure that is altered in dividing somatic cells is telomere length—the terminal ends of linear chromosomes capped by repetitive sequences of G-rich noncoding DNA, (TTAGGG)., and specif作者: gratify 時(shí)間: 2025-3-30 22:21
Pluripotency: ,,ed by two unique properties, unlimited self-renewal capacity and the ability to differentiate into all cells of the body. Because of the high in vitro differentiation potential, ES cells have been used as model system in cell and developmental biology. Here we present methods that use mouse embryoni作者: 暫停,間歇 時(shí)間: 2025-3-31 02:21 作者: 殘暴 時(shí)間: 2025-3-31 07:00 作者: Suggestions 時(shí)間: 2025-3-31 10:08
Methylation-Sensitive Polymerase Chain Reaction,peat sequences of individual pre-implantation ovine embryos produced by different embryo technologies. This method allows the comparison of embryos produced by nuclear transfer with other production and embryo culture methods, accounting for the heterogeneity between embryos within a single treatmen作者: 放肆的我 時(shí)間: 2025-3-31 14:01 作者: ACTIN 時(shí)間: 2025-3-31 19:20 作者: poliosis 時(shí)間: 2025-4-1 00:54 作者: Melodrama 時(shí)間: 2025-4-1 02:19 作者: Valves 時(shí)間: 2025-4-1 06:34 作者: DOLT 時(shí)間: 2025-4-1 12:29 作者: Hdl348 時(shí)間: 2025-4-1 18:03
Rong Wu,Anna V. Terry,David M. Gilbertrtually unknown and/or have never been studied in English.UsThis book examines utopias in classical political economy and is based on the papers presented by leading scholars at the 22nd Heilbronn Symposium in the Economics and the Social Sciences.The book focuses on the tension between the State an作者: 不愿 時(shí)間: 2025-4-1 18:35
Dean H. Betts,Steven Perrault,Lea Harrington,W. Allan Kingthe Economics and the Social Sciences.The book focuses on the tension between the State and utopia (the State as utopia vs. utopia instead of a state). The contributors also study the question of whether seafaring and landlocked states visualize the commonwealth differently and develop different uto
