標(biāo)題: Titlebook: Notch Signaling; Methods and Protocol Hugo J. Bellen,Shinya Yamamoto Book 2014 Springer Science+Business Media New York 2014 Notch activity [打印本頁(yè)] 作者: 瘦削 時(shí)間: 2025-3-21 19:20
書(shū)目名稱(chēng)Notch Signaling影響因子(影響力)
作者: 發(fā)芽 時(shí)間: 2025-3-22 00:08
978-1-4939-5510-7Springer Science+Business Media New York 2014作者: Torrid 時(shí)間: 2025-3-22 01:53
Notch Signaling978-1-4939-1139-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 鳴叫 時(shí)間: 2025-3-22 07:01 作者: tariff 時(shí)間: 2025-3-22 09:44
https://doi.org/10.1007/978-1-4939-1139-4Notch activity in cells; Notch interactome; Notch signaling; genetic methods; image Notch signaling; post作者: 成績(jī)上升 時(shí)間: 2025-3-22 15:27 作者: 驚奇 時(shí)間: 2025-3-22 17:53
,Assay to Probe Proteolytic Processing of Notch by γ-Secretase,s become important. Here we describe a cell-based method to analyze the cleavage of Notch at the S3 site by γ-secretase. HEK cells are transfected with an N-terminal truncated and myc-labeled mNotchΔE construct which can be easily and quantitatively detected by western blotting.作者: 易發(fā)怒 時(shí)間: 2025-3-22 22:56
editor. The problem of random walk as it is originally stated by Pearson (Nature 72:294–294, 1905a) applied to Donsker’s (Mem Am Math Soc 6:1–10, 1951) invariance principle as a functional extension of central limit theorem. The Kalman filter approach together with its extended dynamic version capt作者: Dictation 時(shí)間: 2025-3-23 02:33
Shinya Yamamoto,Karen L. Schulze,Hugo J. Belleneasure, which was the result of a compromise between Congress and the Truman administration, threatened the . of multilateral cooperation between the United States and its Western European allies in East-West trade policy. Forcing nations receiving economic and military assistance from Washington to作者: Clinch 時(shí)間: 2025-3-23 07:10 作者: intimate 時(shí)間: 2025-3-23 11:32
Jessica Leonardi,Hamed Jafar-Nejadmic Consequences of University cosponsored a conference on Government Deficits. " This was the sixth annual Economic Policy Con- ference sponsored by the Center, and the first it has cosponsored with the Institute. This book contains the papers and comments delivered at that conference. Recent and p作者: Biomarker 時(shí)間: 2025-3-23 15:43 作者: 鋪?zhàn)?nbsp; 時(shí)間: 2025-3-23 21:58 作者: 付出 時(shí)間: 2025-3-24 01:36
Lydie Couturier,Fran?ois Schweisguth"The Economic Consequences of University cosponsored a conference on Government Deficits. " This was the sixth annual Economic Policy Con- ference sponsored by the Center, and the first it has cosponsored with the Institute. This book contains the papers and comments delivered at that conference. Re作者: 得意人 時(shí)間: 2025-3-24 03:06 作者: 遷移 時(shí)間: 2025-3-24 09:15
Benjamin E. Housden,Jinghua Li,Sarah J. Brays, deficits as a proportion of GNP, or full employment equivalent deficits are more appropriate measures of fiscal distress and, in turn, are less problematic than the nominal level would suggest. This qualification aside, however, current nominal deficits and, ., those that are forecast for the nex作者: 新陳代謝 時(shí)間: 2025-3-24 12:37
Swananda Marathe,Lavinia Alberito Germany from Turkey, (the former) Yugoslavia, Italy, Greece and Spain in the late sixties and early seventies, when the federal labor office was actively hiring guest workers through recruitment treaties. At that time it was expected, both by the German authorities and by the guest workers themse作者: PRE 時(shí)間: 2025-3-24 16:09
Jinghua Li,Benjamin E. Housden,Sarah J. Brayto Germany from Turkey, (the former) Yugoslavia, Italy, Greece and Spain in the late sixties and early seventies, when the federal labor office was actively hiring guest workers through recruitment treaties. At that time it was expected, both by the German authorities and by the guest workers themse作者: 領(lǐng)先 時(shí)間: 2025-3-24 21:47
Ma. Xenia G. Ilagan,Raphael Kopandate account of Keynes‘ impact on the evolution of internati.First published in December 1919, this global bestseller attacking those who had made the peace in Paris after the First World War, sparked immediate controversy. It also made John Maynard Keynes famous overnight and soon came to define ho作者: BIPED 時(shí)間: 2025-3-24 23:40
Ma. Xenia G. Ilagan,Raphael Kopanbeing destroyed the practical influence of economic theory.” That seems not unlike the situation at the moment. A heated discussion between rival schools has been going on in the blogosphere; of this, hardly an echo appears even in the financial press. The foremost battle concerns the effects of the作者: Airtight 時(shí)間: 2025-3-25 03:42 作者: HEAVY 時(shí)間: 2025-3-25 07:46 作者: 中和 時(shí)間: 2025-3-25 13:23
Pat Whiteman,Christina Redfield,Penny A. Handfordime mortgage default problem in the United States has created the greatest economic catastrophe since the Great Depression. As I pointed out in two recent articles (Davidson, 2008a,. Davidson 2008b,.) it is the deregulation of the financial system that began in the 1970s in the United States that is作者: jagged 時(shí)間: 2025-3-25 16:15
Shinako Kakuda,Robert S. Haltiwangerew theories and models are singularly useful in explaining and understanding the ongoing credit crises. Moreover, it argues that the understanding based on these theories has significant policy implications about how the crises may be alleviated. While the formal articulation of these theories took 作者: generic 時(shí)間: 2025-3-25 22:19 作者: Mawkish 時(shí)間: 2025-3-26 03:21 作者: SPALL 時(shí)間: 2025-3-26 05:48 作者: 欲望小妹 時(shí)間: 2025-3-26 12:05 作者: 谷物 時(shí)間: 2025-3-26 16:01 作者: ciliary-body 時(shí)間: 2025-3-26 18:48
Immunohistochemical Tools and Techniques to Visualize Notch in ,,s during tissue development and homeostasis. Here we describe the procedure to visualize Notch localization in whole-mount preparations of several . organs using confocal microscopy. The use of monoclonal antibodies directed to distinct portions of Notch allows one to follow the fate of the receptor作者: 威脅你 時(shí)間: 2025-3-27 00:32 作者: Ascendancy 時(shí)間: 2025-3-27 03:43
Tracking Trafficking of Notch and Its Ligands in Mammalian Cells,n Notch signaling activation and modulation. In mammalian cultured cells it is possible to track these cell surface molecules by pulse-labeling these proteins in vivo. One labeling protocol consists in the covalent linkage of membrane-impermeable biotin followed by western blotting. An alternative p作者: 清醒 時(shí)間: 2025-3-27 08:27
Visualizing Notch Signaling In Vivo in , Tissues, have been developed to enable monitoring of pathway activity in ., including endogenous Notch-responsive genes and synthetic transcriptional reporter constructs. Here we summarize some of the different Notch signaling reporters that are available, discuss their relative merits, and describe two met作者: sultry 時(shí)間: 2025-3-27 11:43
Monitoring Notch Activity in the Mouse,ifying Notch signaling with high temporal and spatial resolution. This research has contributed substantially to our understanding of the role of Notch in cell specification and cellular physiology. Here, we present two protocols to monitor Notch activity in the mouse brain: (1) by intraventricular 作者: 食物 時(shí)間: 2025-3-27 15:17
Notch Signaling Assays in , Cultured Cell Lines,sessing whether specific enhancers or genes are regulated by Notch. In this chapter, we will describe two different types of assays that can be used to monitor Notch activation in Kc167 and S2 cells. One involves activating Notch in cultured cells and measuring the change in endogenous gene expressi作者: Geyser 時(shí)間: 2025-3-27 17:58
Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays,rive reporter gene expression provides a flexible and robust approach for monitoring signaling activity. In this chapter, we present an overview of Notch transcriptional reporter assays and discuss different methods for ligand-independent and ligand-dependent activation of Notch in mammalian cells.作者: Panacea 時(shí)間: 2025-3-27 22:41
Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays,ing partner CSL to recruit the coactivator MAML and additional cofactors to ultimately activate target gene expression. Taking advantage of the specific interaction between NICD and these factors, we have developed a luciferase complementation imaging (LCI)-based reporter system to quantitatively mo作者: Nostalgia 時(shí)間: 2025-3-28 03:22 作者: 完成才能戰(zhàn)勝 時(shí)間: 2025-3-28 09:36
Proteomic Analysis of the Notch Interactome,r several model organisms. Defining protein interactions on a proteome-wide scale has led to a better understanding of the cellular functions of many proteins, especially those that have not been studied by classical molecular genetic approaches. Here we describe the resources, methods, and techniqu作者: exquisite 時(shí)間: 2025-3-28 13:52 作者: 頌揚(yáng)本人 時(shí)間: 2025-3-28 18:05
Analyzing the Posttranslational Modification Status of Notch Using Mass Spectrometry,D) is modified with .glycosylation and at least three types of .glycosylation (.fucose, .glucose, and .GlcNAc), while the intracellular domain is hydroxylated, phosphorylated, and ubiquitinated. In order to analyze the structure and function of the .glycans decorating the ECD, we have developed semi作者: 針葉類(lèi)的樹(shù) 時(shí)間: 2025-3-28 21:27 作者: 發(fā)出眩目光芒 時(shí)間: 2025-3-29 01:07 作者: chemoprevention 時(shí)間: 2025-3-29 04:08
Identifying Direct Notch Transcriptional Targets Using the GSI-Washout Assay,wever these techniques lack the ability to resolve primary and secondary transcriptional events. In contrast, the γ-secretase inhibitor (GSI) washout assay takes advantage of the reversibility of GSI, a pharmacological inhibitor of Notch signaling, along with the ability of cycloheximide to prevent 作者: CAGE 時(shí)間: 2025-3-29 10:42
1064-3745 key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical,. Notch Signaling: Methods and Protocols. is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor. .978-1-4939-5510-7978-1-4939-1139-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: machination 時(shí)間: 2025-3-29 11:52
Introduction to Notch Signaling,al disorders, stroke, and especially cancer. Pharmacological studies are actively pursuing key components of the pathway as drug targets for potential therapy. In this chapter, we will provide a brief historical overview of Notch signaling research and discuss the basic principles of Notch signaling作者: 發(fā)源 時(shí)間: 2025-3-29 16:37
Genetic Screens to Identify New Notch Pathway Mutants in ,,long with clonal analysis via FRT-mediated mitotic recombination. These F1 clonal screens allow rapid phenotypic screening within clones of mutant cells induced at specific developmental stages and in tissues of interest, bypassing the pleiotropic effects of isolated mutations. More importantly, sin作者: 碎石 時(shí)間: 2025-3-29 20:13 作者: 鑲嵌細(xì)工 時(shí)間: 2025-3-30 02:31
Emiliana Tognon,Thomas Vaccariuents, and bureaus, facilitating their grazing rights and opportunities. There is little incentive to economize on the use of this common resource, since no political actor has to fear being held responsible for the problem. The common pool is a common(s) problem.作者: Salivary-Gland 時(shí)間: 2025-3-30 04:13 作者: 脾氣暴躁的人 時(shí)間: 2025-3-30 10:07
Patricia Chastagner,Christel Brouuents, and bureaus, facilitating their grazing rights and opportunities. There is little incentive to economize on the use of this common resource, since no political actor has to fear being held responsible for the problem. The common pool is a common(s) problem.作者: Relinquish 時(shí)間: 2025-3-30 16:25
Benjamin E. Housden,Jinghua Li,Sarah J. Brayuents, and bureaus, facilitating their grazing rights and opportunities. There is little incentive to economize on the use of this common resource, since no political actor has to fear being held responsible for the problem. The common pool is a common(s) problem.作者: FACET 時(shí)間: 2025-3-30 19:00 作者: 草率女 時(shí)間: 2025-3-30 23:32 作者: reserve 時(shí)間: 2025-3-31 03:20 作者: 熄滅 時(shí)間: 2025-3-31 07:04 作者: 全神貫注于 時(shí)間: 2025-3-31 10:57
K. G. Guruharsha,Kazuya Hori,Robert A. Obar,Spyros Artavanis-Tsakonast this is a re-run of the debates about policies for the Great Depression. Keynes wrote his . to refute the “Treasury View” of the 1920s that the only effect of public spending was to “crowd out” private spending.作者: 使害怕 時(shí)間: 2025-3-31 14:56 作者: 鍵琴 時(shí)間: 2025-3-31 18:31
Shinako Kakuda,Robert S. Haltiwangersion making under conditions of uncertainty it is often the case that the decision maker’s knowledge about the likelihood of contingent events is consistent with more than one probability distribution.作者: calamity 時(shí)間: 2025-4-1 00:02 作者: Macronutrients 時(shí)間: 2025-4-1 05:46 作者: NADIR 時(shí)間: 2025-4-1 06:01 作者: GROSS 時(shí)間: 2025-4-1 12:01
Shinya Yamamoto,Karen L. Schulze,Hugo J. Bellenroduced a major review of the direction and scope of policy in CoCom. Yet when faced with the decision of whether or not to terminate military assistance to allies that violated the Battle Act provisions, the American government chose to preserve the unity of the Western alliance instead of placating a hostile Congress and public opinion.作者: Stress-Fracture 時(shí)間: 2025-4-1 15:56 作者: 西瓜 時(shí)間: 2025-4-1 20:48
