標(biāo)題: Titlebook: Nonribosomal Peptide and Polyketide Biosynthesis; Methods and Protocol Bradley S. Evans Book 2016 Springer Science+Business Media New York [打印本頁(yè)] 作者: 手鐲 時(shí)間: 2025-3-21 19:05
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis影響因子(影響力)
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis影響因子(影響力)學(xué)科排名
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書目名稱Nonribosomal Peptide and Polyketide Biosynthesis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis被引頻次
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis被引頻次學(xué)科排名
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis年度引用
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis年度引用學(xué)科排名
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis讀者反饋
書目名稱Nonribosomal Peptide and Polyketide Biosynthesis讀者反饋學(xué)科排名
作者: COM 時(shí)間: 2025-3-21 23:36
Marisa Till,Paul R. Race to find ways to manage policy better from the centre (see Seidman 1998; Arnold 2000), the various coordination mechanisms that have been created rarely have been sufficiently powerful to overcome the divisions that exist within the administrative system.作者: Heterodoxy 時(shí)間: 2025-3-22 00:44
Swedish administrative culture has been described as ‘cooperative, consensus seeking, problem oriented and pragmatic and is characterized by a high degree of informality, bridging organizational borders and hierarchical levels’ (Hustedt and Tiessen 2006: 38).作者: HERE 時(shí)間: 2025-3-22 05:54 作者: 北京人起源 時(shí)間: 2025-3-22 12:33 作者: byline 時(shí)間: 2025-3-22 14:40
Chitose Maruyama,Haruka Niikura,Masahiro Takakuwa,Hajime Katano,Yoshimitsu HamanoSwedish administrative culture has been described as ‘cooperative, consensus seeking, problem oriented and pragmatic and is characterized by a high degree of informality, bridging organizational borders and hierarchical levels’ (Hustedt and Tiessen 2006: 38).作者: angiography 時(shí)間: 2025-3-22 19:47
Ralph A. Cacho,Yi Tangal, and intellectual – allowed Niels Bohr to establish this “Mecca” of quantum theory outside of traditional and more powerful centres of science. Transitory international postdoctoral fellows, rather than established professors, developed a culture of research that became the source of major innova作者: 收到 時(shí)間: 2025-3-22 22:13
Haoran Zhang,Lei Fang,Marcia S. Osburne,Blaine A. Pfeifermportant issues of surgical approach, bearing options, acetabular preparation and, cup orientation and fixation. The final part focuses on patient management and includes a collection of standard and complex clinical cases to which surgeons can refer when planning surgery..978-3-662-51989-9978-3-642-18396-6作者: 有害處 時(shí)間: 2025-3-23 05:27 作者: neologism 時(shí)間: 2025-3-23 08:52 作者: Painstaking 時(shí)間: 2025-3-23 12:55
Delphine Debois,Marc Ongena,Hélène Cawoy,Edwin De Pauwmportant issues of surgical approach, bearing options, acetabular preparation and, cup orientation and fixation. The final part focuses on patient management and includes a collection of standard and complex clinical cases to which surgeons can refer when planning surgery..978-3-662-51989-9978-3-642-18396-6作者: intrude 時(shí)間: 2025-3-23 15:07 作者: 吞吞吐吐 時(shí)間: 2025-3-23 18:44 作者: 身心疲憊 時(shí)間: 2025-3-24 00:22 作者: acclimate 時(shí)間: 2025-3-24 04:26
Valérie Leclère,Tilmann Weber,Philippe Jacques,Maude Pupinmportant issues of surgical approach, bearing options, acetabular preparation and, cup orientation and fixation. The final part focuses on patient management and includes a collection of standard and complex clinical cases to which surgeons can refer when planning surgery..978-3-662-51989-9978-3-642-18396-6作者: 變異 時(shí)間: 2025-3-24 08:48 作者: 載貨清單 時(shí)間: 2025-3-24 11:45
Guillermin Agüero-Chapin,Gisselle Pérez-Machado,Aminael Sánchez-Rodríguez,Miguel Machado Santos,Agosmportant issues of surgical approach, bearing options, acetabular preparation and, cup orientation and fixation. The final part focuses on patient management and includes a collection of standard and complex clinical cases to which surgeons can refer when planning surgery..978-3-662-51989-9978-3-642-18396-6作者: CIS 時(shí)間: 2025-3-24 14:59 作者: 招募 時(shí)間: 2025-3-24 21:58
Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytocrrier protein domains. This route utilizes Fmoc-chemistry suppressing epimerization of racemization-prone aryl glycine derivatives and affords high yields and excellent purities, requiring only a single step of simple solid phase extraction for chromatographic purification. With this, comprehensive 作者: 四目在模仿 時(shí)間: 2025-3-25 00:56 作者: assail 時(shí)間: 2025-3-25 07:11 作者: 蹣跚 時(shí)間: 2025-3-25 08:54 作者: 疲憊的老馬 時(shí)間: 2025-3-25 12:45
Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domainsrs), which in a first step uses protein four-color maps to represent A-domains. In a second step, simple topological indices (TIs), called spectral moments, are derived from the graphical representations of known A-domains (positive dataset) and of unrelated but well-characterized sequences (negativ作者: Parallel 時(shí)間: 2025-3-25 17:38 作者: Eeg332 時(shí)間: 2025-3-25 21:01 作者: Flatus 時(shí)間: 2025-3-26 04:02
1064-3745 rence for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field..978-1-4939-8034-5978-1-4939-3375-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: FIG 時(shí)間: 2025-3-26 04:41 作者: Decibel 時(shí)間: 2025-3-26 11:20
The Assembly Line Enzymology of Polyketide Biosynthesis, agrochemicals, and veterinary medicines. In bacteria complex polyketides are biosynthesized by giant multifunctional megaenzymes, termed modular polyketide synthases (PKSs), which construct their products in a highly coordinated assembly line-like fashion from a pool of simple precursor substrates作者: 混亂生活 時(shí)間: 2025-3-26 14:14
Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydrox are considered the gatekeepers of NRPSs since they select, activate, and load the carboxylic acid substrate onto a downstream peptidyl carrier protein (PCP) domain of the NRPS. We describe a coupled continuous kinetic assay for NRPS adenylation domains that substitutes the PCP domain with hydroxyla作者: 窗簾等 時(shí)間: 2025-3-26 20:35
Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Usi adenylation (A) domains in nonribosomal peptide synthetases (NRPSs). We recently developed an affinity probe for A domains that could be used to facilitate the specific isolation and identification of NRPS modules. Our synthetic probe, which is a biotinylated variant of .-Phe-AMS (.-Phe-AMS-biotin)作者: Inexorable 時(shí)間: 2025-3-27 00:12
Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetasesomains determine the amino-acid building blocks to be incorporated during nonribosomal peptide biosynthesis. The A-domains mediate ATP-dependent activation of amino-acid substrates as aminoacyl-.-AMP with pyrophosphate (PPi) release. Traditionally, the enzymatic activity of the A-domains has been me作者: 錢財(cái) 時(shí)間: 2025-3-27 02:27
Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytocds all stems from in vivo biosynthesis, understanding the mechanisms of the natural assembly system—consisting of a nonribosomal-peptide synthetase machinery (NRPS) and further modifying enzymes—is vital. In order to address the later steps of peptide biosynthesis, which are catalyzed by Cytochrome 作者: Fsh238 時(shí)間: 2025-3-27 06:25
Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In VitroNRPSs). However, there remain challenges for the characterization of these megasynthases. While genetic methods in native hosts are critical in elucidation of the function of fungal NRPS, in vitro assays of intact heterologously expressed proteins provide deeper mechanistic insights in NRPS enzymolo作者: Ergots 時(shí)間: 2025-3-27 12:12
The Continuing Development of , as a Heterologous Host for Complex Natural Product Biosynthesisn motivated by limitations inherent in native production hosts, the heterologous biosynthetic process begins with a candidate host regarded as technically advanced relative to original producing organisms. Given this requirement, . has been a top choice for heterologous biosynthesis attempts as asso作者: 有害處 時(shí)間: 2025-3-27 13:45
Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC-MS/MS-Basroughput fashion. Herein we report a protocol using LC-MS/MS-based proteomics for the screening of enzymes involved in natural product biosynthesis, such as nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) from bacterial strains. Taking advantage of the large size of modular 作者: 說明 時(shí)間: 2025-3-27 18:04 作者: 冬眠 時(shí)間: 2025-3-27 22:07
In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionizationnal mapping of a broad variety of biomolecules present at the surface of a sample. In many applications including pharmaceutical studies or biomarker discovery, the distribution of proteins, lipids or drugs, and metabolites may be visualized within tissue sections. More recently, MALDI MSI has becom作者: 抵押貸款 時(shí)間: 2025-3-28 04:57 作者: 不吉祥的女人 時(shí)間: 2025-3-28 07:12 作者: 食草 時(shí)間: 2025-3-28 13:59 作者: ungainly 時(shí)間: 2025-3-28 17:06
The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathwolyketide and nonribosomal peptide biosynthetic pathways have been characterized leading to a surfeit of genetic data on polyketide and nonribosomal peptide biosynthesis. We developed the ClusterMine360 database, which stores the antiSMASH-based annotation of gene clusters in the NCBI database, link作者: 財(cái)政 時(shí)間: 2025-3-28 20:15
Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains/proteome level and allow inferring the structure of oligopeptides with relevant biological activities. However, that is challenging task due to the high sequence diversity of A-domains (~10–40 % of amino acid identity) and their selectivity for 50 different natural/unnatural amino acids. Altogether作者: 暫時(shí)別動(dòng) 時(shí)間: 2025-3-29 00:00 作者: 規(guī)范要多 時(shí)間: 2025-3-29 05:50 作者: 碎片 時(shí)間: 2025-3-29 10:09
ective decision-making, producing a system ‘which is collective in the extreme and individual ministers may decide themselves only in exceptional circumstances’ (Larsson 1995). The government — not individual ministers — issues directives to administration, although the individual minister can take 作者: accordance 時(shí)間: 2025-3-29 14:41 作者: Retrieval 時(shí)間: 2025-3-29 16:50
Fumihiro Ishikawa,Hideaki Kakeya effective coordination than do governments in other countries. The American political system was designed by its founding fathers. to be ‘a(chǎn)ll anchor and no sail’. To that institutionally divided and highly contentious political system has been added a bureaucratic system that is itself very divided作者: breadth 時(shí)間: 2025-3-29 22:58 作者: DAMP 時(shí)間: 2025-3-30 01:26 作者: 通便 時(shí)間: 2025-3-30 06:49
Ralph A. Cacho,Yi Tang, but of a recent or actual Ph.D. student just embarking on an uncertain academic career in economically hard times. Quantum mechanics exploded on to the intellectual scene between 1925 and 1927, with more than 200 publications across the world, the majority of them authored by young scientists unde作者: parallelism 時(shí)間: 2025-3-30 10:25
Haoran Zhang,Lei Fang,Marcia S. Osburne,Blaine A. Pfeiferienced surgeons.Discusses in detail all practical aspects of.The Corail? Hip System was developed in 1986 as an innovative solution for hip arthroplasty and has since become one of the most used hip systems in the world. This book is designed as a practical manual to primary and revision arthroplast作者: debacle 時(shí)間: 2025-3-30 13:13
Yunqiu Chen,Ryan A. McClure,Neil L. Kelleherienced surgeons.Discusses in detail all practical aspects of.The Corail? Hip System was developed in 1986 as an innovative solution for hip arthroplasty and has since become one of the most used hip systems in the world. This book is designed as a practical manual to primary and revision arthroplast作者: languor 時(shí)間: 2025-3-30 18:04 作者: Defense 時(shí)間: 2025-3-30 23:05 作者: 使害羞 時(shí)間: 2025-3-31 01:05
Maria I. Vizcaino,Jason M. Crawfordienced surgeons.Discusses in detail all practical aspects of.The Corail? Hip System was developed in 1986 as an innovative solution for hip arthroplasty and has since become one of the most used hip systems in the world. This book is designed as a practical manual to primary and revision arthroplast作者: Conserve 時(shí)間: 2025-3-31 07:51
