作者: mortuary 時間: 2025-3-21 23:40
Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Usiminoacyl-tRNA synthetase (aaRS) pairs from ., we have developed CFPS methods for the highly productive and efficient multiple incorporation of ncAAs. In this chapter, we describe our methods for the preparation of the S30 and the orthogonal tRNA. and PylRS pair, and two CFPS protocols for ncAA incorporation.作者: RENAL 時間: 2025-3-22 02:24 作者: monogamy 時間: 2025-3-22 07:43
Site-Specific Incorporation of Sulfotyrosine Using an Expanded Genetic Codeas noncanonical amino acids (ncAAs) encoded by an expanded genetic code. Here, we describe methods for the co-translational incorporation of one or multiple sulfotyrosines into proteins using standard or genomically recoded . stains, thereby achieving the direct expression of site-specifically tyrosine sulfated proteins in vivo.作者: 謊言 時間: 2025-3-22 11:05
Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivll cycle in synchronized yeast populations. This method thereby provides unprecedented insights into the molecular events controlling chromatin reorganization in mitosis. As similar tools are available for other organisms, it should be possible to derive similar strategies for these and for other synchronizable processes.作者: 確保 時間: 2025-3-22 16:49 作者: SPASM 時間: 2025-3-22 19:54 作者: BULLY 時間: 2025-3-22 22:14
Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering handles (azides and alkynes) and hydrophobic fluorinated side chains. Here, we describe the efficient residue-specific replacement of methionine by azidonorleucine in an engineered green fluorescent protein using a bacterial expression system to introduce a single reactive site for the strain-promoted azide-alkyne cycloaddition.作者: syncope 時間: 2025-3-23 01:56 作者: Parallel 時間: 2025-3-23 07:44 作者: 公式 時間: 2025-3-23 13:05
Generation of Stable Amber Suppression Cell Linesediated transposition. A general protocol for the generation of stable amber suppression cell lines is described here. Using the modular plasmid system, homogenous and highly efficient amber suppression in a wide range of cell lines can be achieved.作者: 弄臟 時間: 2025-3-23 17:22 作者: 谷類 時間: 2025-3-23 19:17 作者: osteoclasts 時間: 2025-3-24 00:55
978-1-4939-8525-8Springer Science+Business Media, LLC 2018作者: Climate 時間: 2025-3-24 06:25 作者: 雪上輕舟飛過 時間: 2025-3-24 06:36 作者: 壟斷 時間: 2025-3-24 13:18
Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/667175.jpg作者: 孤獨(dú)無助 時間: 2025-3-24 18:46 作者: DOSE 時間: 2025-3-24 22:37 作者: 冷淡一切 時間: 2025-3-25 02:27
Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugatemylglycine-generating enzyme (FGE) is one enzyme that can be exploited in this fashion. FGE binds its consensus sequence (CXPXR, known as the “aldehyde-tag”) and converts the cysteine to a formylglycine (fGly). fGly-containing proteins contain a bioorthogonal aldehyde on their surface that can be mo作者: 不幸的人 時間: 2025-3-25 04:52
Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypepticodon boxes consisting of synonymous codons that redundantly code for the same amino acid. Here, we describe the protocol of a recently published method to artificially divide such family codon boxes and encode multiple nonproteinogenic amino acids in addition to the 20 proteinogenic ones in a repro作者: irreparable 時間: 2025-3-25 08:31 作者: 噱頭 時間: 2025-3-25 14:32
Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acidsrporates noncanonical tyrosine derivatives to the terminal carboxylic acid of proteins containing a 14-amino acid recognition sequence called Tub-tag. The tyrosine derivative carries a unique chemical reporter allowing for a subsequent bioorthogonal modification of proteins with a great variety of p作者: BLANC 時間: 2025-3-25 18:13 作者: 切割 時間: 2025-3-25 21:41
Genetic Code Expansion in Enteric Bacterial Pathogensies into proteins of interest in bacteria, yeast, mammalian cells, and even animals. This technique allows precise labeling as well as manipulation of a given protein to dissect its physiological and/or pathological roles under living conditions. Here, we demonstrate the extension of a recently emer作者: 物種起源 時間: 2025-3-26 03:43 作者: Bricklayer 時間: 2025-3-26 08:08
Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineeringcantly broadened the scope of protein engineering allowing introduction of amino acids with non-native functionalities, such as bioorthogonal reactive handles (azides and alkynes) and hydrophobic fluorinated side chains. Here, we describe the efficient residue-specific replacement of methionine by a作者: 妨礙 時間: 2025-3-26 11:04 作者: 感情脆弱 時間: 2025-3-26 14:29 作者: 綁架 時間: 2025-3-26 19:11 作者: Affable 時間: 2025-3-26 23:17
Site-Specific Incorporation of Sulfotyrosine Using an Expanded Genetic Codeon of homogenously sulfated proteins is particularly difficult, limiting our study and application of tyrosine-sulfated proteins. With the recent development of genomically recoded organisms and orthogonal translation components, we can often treat otherwise posttranslationally-modified amino acids 作者: nugatory 時間: 2025-3-27 03:44 作者: nauseate 時間: 2025-3-27 09:02
Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkersas X-ray crystallography and nuclear magnetic resonance can investigate in detail only isolated protein complexes that are reconstituted in an artificial environment. Alternative methods are needed to investigate protein interactions in a physiological context, as well as to characterize protein com作者: coddle 時間: 2025-3-27 10:52
Generation of Stable Amber Suppression Cell Lineso acids have been incorporated using the . pyrrolysyl-tRNA synthetase/tRNACUA (PylRS/PylT) in mammalian cell systems in proof of principle experiments, for (1)?minimal genetically encoded fluorescence or affinity tagging, (2) photo-control of enzymes, (3) genetically encoded posttranslational protei作者: Delude 時間: 2025-3-27 13:44
Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Viv UV-activatable crosslinker amino acids allow the formation of a covalent bond between interaction partners in living cells with nearly perfect spatial and temporal control. Here, we describe how this method can be employed to map chromatin interactions and to follow these interactions across the ce作者: 尾隨 時間: 2025-3-27 19:53 作者: incisive 時間: 2025-3-27 23:49
zu den 1.500 meistverwendeten Arzneimitteln.Detaillierte Inf.Haben Sie Fragen zu Arzneimittelauswahl und Therapieführung? Die 13. Auflage des Taschenbuchs der Arzneibehandlung bietet zuverl?ssige Informationen zur genauen Beurteilung der therapeutischen Wirkung der 1.500 meistverwendeten Arzneimitte作者: insightful 時間: 2025-3-28 04:24
Robyn M. Barfield,David Rabukazu den 1.500 meistverwendeten Arzneimitteln.Detaillierte Inf.Haben Sie Fragen zu Arzneimittelauswahl und Therapieführung? Die 13. Auflage des Taschenbuchs der Arzneibehandlung bietet zuverl?ssige Informationen zur genauen Beurteilung der therapeutischen Wirkung der 1.500 meistverwendeten Arzneimitte作者: 流出 時間: 2025-3-28 09:06
Yoshihiko Iwane,Takayuki Katoh,Yuki Goto,Hiroaki Sugazu den 1.500 meistverwendeten Arzneimitteln.Detaillierte Inf.Haben Sie Fragen zu Arzneimittelauswahl und Therapieführung? Die 13. Auflage des Taschenbuchs der Arzneibehandlung bietet zuverl?ssige Informationen zur genauen Beurteilung der therapeutischen Wirkung der 1.500 meistverwendeten Arzneimitte作者: Lipoprotein 時間: 2025-3-28 13:13
Eiko Seki,Tatsuo Yanagisawa,Shigeyuki Yokoyamazu den 1.500 meistverwendeten Arzneimitteln.Detaillierte Inf.Haben Sie Fragen zu Arzneimittelauswahl und Therapieführung? Die 13. Auflage des Taschenbuchs der Arzneibehandlung bietet zuverl?ssige Informationen zur genauen Beurteilung der therapeutischen Wirkung der 1.500 meistverwendeten Arzneimitte作者: 寵愛 時間: 2025-3-28 15:40 作者: BRIBE 時間: 2025-3-28 19:38
zu den 1.500 meistverwendeten Arzneimitteln.Detaillierte Inf.Haben Sie Fragen zu Arzneimittelauswahl und Therapieführung? Die 13. Auflage des Taschenbuchs der Arzneibehandlung bietet zuverl?ssige Informationen zur genauen Beurteilung der therapeutischen Wirkung der 1.500 meistverwendeten Arzneimitte作者: Ligneous 時間: 2025-3-29 00:59 作者: febrile 時間: 2025-3-29 04:03 作者: nurture 時間: 2025-3-29 08:44
Ying Ma,Martino L. Di Salvo,Nediljko Budisach, da? Verkehrsflüsse, die einander gef?hrden würden, das Wegerecht nacheinander erhalten und damit die Kreuzung ungef?hrdet überqueren k?nnen. Dieses Nacheinander hat natürlich zur Folge, da? die Kreuzung einem Verkehrsstrom nur einen Bruchteil der Zeit zur Verfügung steht. überschreitet der Zeitb作者: 聲明 時間: 2025-3-29 13:26 作者: Flirtatious 時間: 2025-3-29 18:43 作者: disparage 時間: 2025-3-29 20:12 作者: murmur 時間: 2025-3-30 02:48
Michael Lammerse problematischste Rolle spielt. Gespeichert werden müssen neben den Eingabedaten, Zwischenergebnissen und Ausgabedaten auch das jeweilige Verarbeitungsprogramm und h?ufig erstaunlich viele Organisationsprogramme. Dies erfordert neben riesiger Speicherkapazit?t bei ertr?glichen Kosten eine mit der h作者: 松果 時間: 2025-3-30 04:47
Xiang Li,Chang C. Liue problematischste Rolle spielt. Gespeichert werden müssen neben den Eingabedaten, Zwischenergebnissen und Ausgabedaten auch das jeweilige Verarbeitungsprogramm und h?ufig erstaunlich viele Organisationsprogramme. Dies erfordert neben riesiger Speicherkapazit?t bei ertr?glichen Kosten eine mit der h作者: PRE 時間: 2025-3-30 11:37
Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptiged with nonproteinogenic amino acids by means of flexizymes, the nonproteinogenic and proteinogenic aminoacyl-tRNAs can decode the NNC and NNG codons in the same family codon box independently. In this protocol, we describe expression of model peptides, including a macrocyclic peptide containing th作者: 破布 時間: 2025-3-30 15:46 作者: 壕溝 時間: 2025-3-30 18:32
Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in , ATCC 15439enables use of other aaRS or aaRS/tRNA pairs for the incorporation of other UAAs; and the vector backbone used allows the system to be transferred to diverse . species via both protoplast transformation and conjugation.作者: 詼諧 時間: 2025-3-30 22:46
Expression and Purification of Site-Specifically Lysine-Acetylated and Natively-Folded Proteins for uding X-ray crystallography. This protocol describes the expression and purification of site-specifically lysine-acetylated proteins in . using the genetic-code expansion concept (GCEC) and subsequent steps to assess the successful incorporation of AcK by immunoblotting and mass-spectrometry.作者: 連鎖,連串 時間: 2025-3-31 01:50 作者: Forehead-Lift 時間: 2025-3-31 08:09
harmakologischen Institutionen. Im Taschenbuch von Schwabe/Scholz finden Sie hilfreiche Informationen für eine rationale und kostenbewusste Arzneitherapie..978-3-540-26502-3Series ISSN 0937-7433 Series E-ISSN 2512-5214 作者: 巫婆 時間: 2025-3-31 11:03
