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標(biāo)題: Titlebook: Non-Viral Gene Delivery Vectors; Methods and Protocol Gabriele Candiani Book 2016 Springer Science+Business Media New York 2016 conventiona [打印本頁]

作者: 方言 時間: 2025-3-21 17:43
書目名稱Non-Viral Gene Delivery Vectors影響因子(影響力)

書目名稱Non-Viral Gene Delivery Vectors影響因子(影響力)學(xué)科排名

書目名稱Non-Viral Gene Delivery Vectors網(wǎng)絡(luò)公開度

書目名稱Non-Viral Gene Delivery Vectors網(wǎng)絡(luò)公開度學(xué)科排名

書目名稱Non-Viral Gene Delivery Vectors被引頻次

書目名稱Non-Viral Gene Delivery Vectors被引頻次學(xué)科排名

書目名稱Non-Viral Gene Delivery Vectors年度引用

書目名稱Non-Viral Gene Delivery Vectors年度引用學(xué)科排名

書目名稱Non-Viral Gene Delivery Vectors讀者反饋

書目名稱Non-Viral Gene Delivery Vectors讀者反饋學(xué)科排名

作者: orient 時間: 2025-3-21 22:30
Quantitative Intracellular Localization of Cationic Lipid–Nucleic Acid Nanoparticles with FluorescenA NPs, perform fluorescence microscopy and properly analyze the data to measure the intracellular distribution of nanoparticles and fluorescent signal. We provide software which allows data from multiple cells to be averaged together and yield statistically significant results. The second method, fl

作者: defendant 時間: 2025-3-22 03:16
Biodegradable Three-Layered Micelles and Injectable HydrogelsEI-based systems. The polyplex micelles have superb stability and stealth properties. Moreover, we describe a method to prepare fully biodegradable and biocompatible injectable hydrogels for use in localized gene therapy.

作者: Hormones 時間: 2025-3-22 06:33
Cationic Lipid-Coated Polyplexes (Lipopolyplexes) for DNA and Small RNA Deliveryns containing different liposomes and certain branched or linear low-molecular weight PEIs are given. This also includes optimal parameters for lipopolyplex generation, based on various PEIs, N/P ratios, lipids, lipid/PEI ratios, and preparation conditions..Importantly, certain lipopolyplexes retain

作者: 橫條 時間: 2025-3-22 11:28

作者: 軍械庫 時間: 2025-3-22 16:47
Book 2016n the highlysuccessful .Methods in Molecular Biology. series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls..Cutting-edge a

作者: Lipoprotein 時間: 2025-3-22 18:56
1064-3745 ductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls..Cutting-edge a978-1-4939-8118-2978-1-4939-3718-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

作者: Barrister 時間: 2025-3-22 22:00
Emile Jubeli,William P. D. Goldring,Michael D. Pungente

作者: 吹牛大王 時間: 2025-3-23 04:22
Edilberto Ojeda,Mireia Agirre,Ilia Villate-Beitia,Mohamed Mashal,Gustavo Puras,Jon Zarate,Jose L. Pe

作者: 廢墟 時間: 2025-3-23 08:07
Danielle Campiol Arruda,Anne Schlegel,Pascal Bigey,Virginie Escriou

作者: murmur 時間: 2025-3-23 12:36
Margarida I. Sim?o Carlos,Andreas Sch?tzlein,Ijeoma Uchegbu

作者: FELON 時間: 2025-3-23 13:53

作者: PATRI 時間: 2025-3-23 18:36

作者: catagen 時間: 2025-3-23 23:20

作者: 不容置疑 時間: 2025-3-24 02:31
Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micellesng interaction with nucleic acid. Herein the main issues related to complex formation and in vitro administration are illustrated with key examples, such as liposome-DNA plasmid (pDNA) association and micelles-siRNA complexes.

作者: abject 時間: 2025-3-24 09:16
Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposome protocols for the elaboration of niosomes and nioplexes and their physicochemical characterization that guarantees the quality criteria of the formulation in terms of size, morphology, ζ-potential, and stability.

作者: aviator 時間: 2025-3-24 14:18
Polymer Based Gene Silencing: In Vitro Delivery of SiRNA). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a polymer are presented in this chapter using EAGC as an example. The steps include: the transfection of cells with EAGC-siRNA polyplexes and protein detection by a Western Blotting assay.

作者: 使乳化 時間: 2025-3-24 17:51

作者: Recess 時間: 2025-3-24 21:55

作者: Immortal 時間: 2025-3-25 01:28
Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA an AL, a zwitterionic lipid (ZL), and a plasmid DNA (pDNA), their electrostatic interaction being necessarily mediated by divalent cations, such as Ca.. In the present chapter, we summarize the protocols optimized for the mentioned characterization techniques.

作者: Dislocation 時間: 2025-3-25 05:55
Polyallylamine Derivatives: Novel NonToxic Transfection Agentsf polyplexes using dynamic light scattering, ζ-potential measurements, gel retardation assay, and atomic force microscopy. Moreover, we provide protocols for the transfection of HeLa cell line by polyplexes, determination of their cytotoxicity, cell uptake, and intracellular trafficking.

作者: Nuance 時間: 2025-3-25 08:33
1064-3745 ation advice from the experts.Includes supplementary materiaThis volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I i

作者: 萬靈丹 時間: 2025-3-25 13:51

作者: 混合 時間: 2025-3-25 17:00
Book 2016 delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II cov

作者: narcotic 時間: 2025-3-26 00:01

作者: Facet-Joints 時間: 2025-3-26 03:25

作者: 謙虛的人 時間: 2025-3-26 07:20
https://doi.org/10.1007/978-1-4939-3718-9conventional bolus gene delivery vectors; cell culture medium; stimuli-responsive bolus transfectants;

作者: metropolitan 時間: 2025-3-26 11:16
Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells corrective gene therapy for the treatment of neoplasms and other disorders of a genetic origin. Here, a step-wise account of the assembly and characterization of hepatocellular carcinoma cell-specific DNA lipoplexes and their cytotoxicity assessment in and delivery to the human hepatocellular carcinoma cell line HepG2 is given.

作者: Fresco 時間: 2025-3-26 12:58

作者: GRACE 時間: 2025-3-26 18:28
Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA Deliverysed on targeted anionic lipid-coated polyplexs for therapeutic delivery of microRNA (miRNA) mimics. These LNPs are particularly suited for therapeutic delivery of oligonucleotide agents to leukemia cells.

作者: MAG 時間: 2025-3-26 23:53
From Artificial Amino Acids to Sequence-Defined Targeted Oligoaminoamideslp of these oligoamino acids multifunctional nucleic acid (NA) carriers can be designed and produced in highly defined topologies. Here we describe the synthesis of the artificial oligoamino acid Fmoc-Stp(Boc.)-OH, the subsequent assembly into sequence-defined oligomers and the formulation of tumor-targeted plasmid DNA (pDNA) polyplexes.

作者: Dungeon 時間: 2025-3-27 02:27

作者: Type-1-Diabetes 時間: 2025-3-27 06:05

作者: BROTH 時間: 2025-3-27 09:33

作者: 沙文主義 時間: 2025-3-27 17:18

作者: Perennial長期的 時間: 2025-3-27 21:31
Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micellesre promising transfection agents and do not suffer from limitations related to toxicity and immunogenic effects. In particular, lipid-based aggregates are generally considered biocompatible and versatile nanocarriers whose composition can be designed to include a cationic molecule which ensures stro

作者: 共和國 時間: 2025-3-27 22:16

作者: Myelin 時間: 2025-3-28 03:27

作者: QUAIL 時間: 2025-3-28 08:12

作者: Alcove 時間: 2025-3-28 13:49
Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells corrective gene therapy for the treatment of neoplasms and other disorders of a genetic origin. Here, a step-wise account of the assembly and characterization of hepatocellular carcinoma cell-specific DNA lipoplexes and their cytotoxicity assessment in and delivery to the human hepatocellular carci

作者: Gingivitis 時間: 2025-3-28 17:47

作者: Rct393 時間: 2025-3-28 20:32
Polymer Based Gene Silencing: In Vitro Delivery of SiRNARNA is dependent on its delivery to the intracellular space. This chapter describes the delivery of siRNA by .-(2-ethylamino)-6-.-glycolchitosan (EAGC). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a pol

作者: acclimate 時間: 2025-3-29 02:28
Polyallylamine Derivatives: Novel NonToxic Transfection Agentse in the context of using cationic polymers as carriers is the balance between the high transfection efficiency and low cytotoxicity. In this chapter, we report the preparation of polyallylamine derivatives mainly based on substitution of amino groups by glycidyltrimethylammonium chloride. The resul

作者: 帶子 時間: 2025-3-29 07:09
Biodegradable Three-Layered Micelles and Injectable Hydrogelscationic polymer polyethylene imine (PEI) has shown relatively high condensation and transfection efficiencies. Additionally, polyethylene glycol (PEG) modification of polymeric gene vectors has dramatically improved their biological properties, including enhanced biocompatibility, prolonged circula

作者: 生氣地 時間: 2025-3-29 09:14