標(biāo)題: Titlebook: Non-Ribosomal Peptide Biosynthesis and Engineering; Methods and Protocol Michael Burkart,Fumihiro Ishikawa Book 2023 The Editor(s) (if appl [打印本頁] 作者: 和善 時(shí)間: 2025-3-21 18:43
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering影響因子(影響力)
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering影響因子(影響力)學(xué)科排名
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering網(wǎng)絡(luò)公開度
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering被引頻次
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering被引頻次學(xué)科排名
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering年度引用
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering年度引用學(xué)科排名
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering讀者反饋
書目名稱Non-Ribosomal Peptide Biosynthesis and Engineering讀者反饋學(xué)科排名
作者: 奇思怪想 時(shí)間: 2025-3-21 22:26 作者: Geyser 時(shí)間: 2025-3-22 03:35 作者: ICLE 時(shí)間: 2025-3-22 05:20
Unraveling Structural Information of Multi-Domain Nonribosomal Peptide Synthetases by Using Photo-Crdy the communication (COM) domains, a special pair of docking domains of unknown structure between two interacting subunits of one NRPS system, this cross-linking approach was also found to be useful to interrogate the spatial proximity of domains that are not connected on the level of the primary s作者: 羞辱 時(shí)間: 2025-3-22 10:07 作者: 比目魚 時(shí)間: 2025-3-22 13:56
Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonancental readouts necessary to assess the integrity of the sample and maintain loading on CPs. Our approach provides a basis to conduct subsequent NMR experiments and obtain kinetic, thermodynamic, dynamic, and structural parameters of substrate-loaded CPs alone or in the presence of other domains.作者: Minutes 時(shí)間: 2025-3-22 19:27
Ketan D. Patel,Syed Fardin Ahmed,Monica R. MacDonald,Andrew M. Gulick作者: 糾纏 時(shí)間: 2025-3-22 23:00
Desirae A. Mellor,Javier O. Sanlley,Michael Burkart作者: 西瓜 時(shí)間: 2025-3-23 03:47 作者: Gratulate 時(shí)間: 2025-3-23 06:50 作者: 痛打 時(shí)間: 2025-3-23 10:47 作者: incite 時(shí)間: 2025-3-23 17:21 作者: Acquired 時(shí)間: 2025-3-23 18:31
Y. T. Candace Ho,Yongwei Zhao,Julien Tailhades,Max J. Cryle作者: 標(biāo)準(zhǔn) 時(shí)間: 2025-3-24 00:11
Nadya Abbood,Leonard Pr?ve,Kenan A. J. Bozhueyuek,Helge B. Bode作者: admission 時(shí)間: 2025-3-24 03:32 作者: Wernickes-area 時(shí)間: 2025-3-24 10:16 作者: CUMB 時(shí)間: 2025-3-24 14:22
1064-3745 s..?Authoritative and cutting-edge, .Non-Ribosomal Peptide Biosynthesis and Engineering: Methods and Protocols .aims to feature methods that will be beneficial to new researchers, and those wanting to adopt new methodologies into their research..978-1-0716-3216-1978-1-0716-3214-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: JIBE 時(shí)間: 2025-3-24 16:57 作者: 分散 時(shí)間: 2025-3-24 21:38 作者: ANNUL 時(shí)間: 2025-3-25 02:19 作者: intimate 時(shí)間: 2025-3-25 05:44
Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/667020.jpg作者: intention 時(shí)間: 2025-3-25 10:10
https://doi.org/10.1007/978-1-0716-3214-7Non-ribosomal peptide synthetases (NRPSs); Peptide natural products; Biosynthetic enzymes; Bioinformati作者: Monotonous 時(shí)間: 2025-3-25 13:18
Norine: Bioinformatics Methods and Tools for the Characterization of Newly Discovered Nonribosomal PIn this chapter, we present Norine (.), the unique resource dedicated to nonribosomal peptides. First, the content of the knowledgebase and the related tools are described. Then, a study case shows how to query Norine by annotations or structure and how to interpret the obtained results.作者: ellagic-acid 時(shí)間: 2025-3-25 16:25
Michael Burkart,Fumihiro IshikawaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: NATAL 時(shí)間: 2025-3-25 21:22 作者: ectropion 時(shí)間: 2025-3-26 02:08
s à partir des travaux de J.J. Kohn..Lorsque la frontière est strictement pseudo-convexe ou analytique réelle, on constate (cf. Chapitre I) que les problèmes les plus importants sont résolus..Soit donc Ω un domaine de ?. faiblement pseudo-convexe à frontière ?...Ce travail porte sur l‘existence de c作者: echnic 時(shí)間: 2025-3-26 06:03
Chitose Maruyama,Yoshimitsu Hamanos à partir des travaux de J.J. Kohn..Lorsque la frontière est strictement pseudo-convexe ou analytique réelle, on constate (cf. Chapitre I) que les problèmes les plus importants sont résolus..Soit donc Ω un domaine de ?. faiblement pseudo-convexe à frontière ?...Ce travail porte sur l‘existence de c作者: Inexorable 時(shí)間: 2025-3-26 12:01 作者: exhibit 時(shí)間: 2025-3-26 15:15 作者: GLUE 時(shí)間: 2025-3-26 20:08 作者: arcane 時(shí)間: 2025-3-26 22:42
Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-Producing Non-ribosomal Pepdenylation (A) domains in NRPSs are responsible for the incorporation of amino acid building blocks and can be considered as engineering domains; therefore, advanced techniques are required to not only rapidly verify expression and folding, but also accelerate the functional prediction of the A-doma作者: 指令 時(shí)間: 2025-3-27 03:36 作者: 松緊帶 時(shí)間: 2025-3-27 07:03
Chemo-Enzymatic Synthesis of Non-ribosomal Macrolactams by a Penicillin-Binding Protein-Type Thioestubstrate scopes from the well-exploited ribosomal peptide cyclases and traditional non-ribosomal peptide cyclases. Their unique properties, as well as their stand-alone nature, highlight PBP-type TEs as valuable candidates for development as biocatalysts for peptide macrocyclization. Here in this ch作者: ACTIN 時(shí)間: 2025-3-27 11:45 作者: Irremediable 時(shí)間: 2025-3-27 17:11
Unraveling Structural Information of Multi-Domain Nonribosomal Peptide Synthetases by Using Photo-Cr serve as important pharmaceutical products. Typically, NRPSs contain one module for the incorporation of one amino acid into the growing peptide chain. A module consists of the domains required for activation, covalent binding, condensation, termination, and optionally modification of the aminoacyl作者: lesion 時(shí)間: 2025-3-27 18:11 作者: dowagers-hump 時(shí)間: 2025-3-28 00:21
Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Usingides and related natural products. The A-domain transfers an acyl substrate onto its cognate carrier protein (CP). The proper interactions between an A-domain and the cognate CP are important for functional substrate transfer. To stabilize the transient interactions sufficiently for structural analy作者: Kindle 時(shí)間: 2025-3-28 04:16
A Practical Guideline to Engineering Nonribosomal Peptide Synthetasesatural products like scaffolds with changed or improved properties. However, the rational (re-)design of these often gigantic assembly-line proteins is by no means trivial and needs in-depth insights into structural flexibility, inter-domain communication, and the role of proofreading by catalytic d作者: 搖曳的微光 時(shí)間: 2025-3-28 07:31
Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonancevalently hold the substrates and intermediates leading to the final product. Thus, how CPs and their partner domains recognize and engage with each other as a function of CP cargos is paramount to understanding and engineering NRPSs. However, rapid hydrolysis of the labile thioester bonds holding su作者: 反感 時(shí)間: 2025-3-28 13:03 作者: 含鐵 時(shí)間: 2025-3-28 15:16 作者: NEX 時(shí)間: 2025-3-28 22:08 作者: 恩惠 時(shí)間: 2025-3-29 02:02
1064-3745 ation advice from the experts.This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistr作者: 轉(zhuǎn)折點(diǎn) 時(shí)間: 2025-3-29 07:04 作者: liaison 時(shí)間: 2025-3-29 08:53 作者: 過剩 時(shí)間: 2025-3-29 15:17 作者: 凹室 時(shí)間: 2025-3-29 18:58
Chemical Labeling of Protein 4′-Phosphopantetheinylation in Surfactin-Producing Nonribosomal Peptidee chemical labeling of 4′-phosphopantethylated NRPSs. In this chapter, we describe the design and synthesis of an activity-based protein profiling probe and summarize our work toward developing a series of protocols for the labeling and visualization of 4′-phosphopantetheinylation of endogenous NRPSs in complex proteomes.作者: 等待 時(shí)間: 2025-3-29 20:37
The Assembly-Line Enzymology of Nonribosomal Peptide Biosynthesise chain before releasing the complete peptide. Adenylation, thiolation, condensation, and thioesterase domains play central roles in these reactions. This chapter focuses on the current understanding of these central domains in NRPS assembly-line enzymology.作者: N防腐劑 時(shí)間: 2025-3-30 01:42 作者: modifier 時(shí)間: 2025-3-30 04:14
Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-Producing Non-ribosomal Pepobust analytical platform for A-domains and provides insights into their enzyme–substrate specificity. In this chapter, we describe the design and synthesis of these ABPP probes and provide a summary of our work on the development of a series of protocols for labeling, visualizing, and analyzing endogenous NRPSs in complex biological systems.作者: RUPT 時(shí)間: 2025-3-30 10:11 作者: 使虛弱 時(shí)間: 2025-3-30 16:11
A Practical Guideline to Engineering Nonribosomal Peptide Synthetasessult of nearly one decade of NRPS engineering in the Bode lab, we provide valuable insights into the strategies we have developed during this time for the successful engineering and cloning of these fascinating molecular machines.作者: 豪華 時(shí)間: 2025-3-30 18:33 作者: 統(tǒng)治人類 時(shí)間: 2025-3-30 21:53
In Vitro Biochemical Characterization of Excised Macrocyclizing Thioesterase Domains from Non-ribosodes substrate required for characterization of the TE. In this method, we describe the cloning and expression of NRPS TEs, the synthesis of thioester peptides, and the . biochemical characterization of the enzyme.作者: 錢財(cái) 時(shí)間: 2025-3-31 04:16
Chemo-Enzymatic Synthesis of Non-ribosomal Macrolactams by a Penicillin-Binding Protein-Type Thioest their stand-alone nature, highlight PBP-type TEs as valuable candidates for development as biocatalysts for peptide macrocyclization. Here in this chapter, we describe the scheme for the chemoenzymatic synthesis of non-ribosomal macrolactam by SurE, a representative member of PBP-type TEs.作者: 侵略主義 時(shí)間: 2025-3-31 07:26
Ribosomal Synthesis of Peptides Bearing Noncanonical Backbone Structures via Chemical Posttranslatiocursor residues and their chemical posttranslational modification processes. In this chapter, we describe the detailed procedures for the in vitro translation of peptides containing the precursor residues by means of genetic code reprogramming technology and posttranslational generation of objective noncanonical backbone structures.作者: 外向者 時(shí)間: 2025-3-31 11:00 作者: minaret 時(shí)間: 2025-3-31 16:18
10樓作者: 放逐 時(shí)間: 2025-3-31 18:44
10樓
