作者: VEST 時(shí)間: 2025-3-21 22:38 作者: 聲音刺耳 時(shí)間: 2025-3-22 02:52 作者: kindred 時(shí)間: 2025-3-22 06:38
1064-3745 known pitfalls.?.Authoritative and cutting-edge, .Non-Natural Nucleic Acids: Methods and Protocols. aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors..978-1-4939-9218-8978-1-4939-9216-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 笨拙處理 時(shí)間: 2025-3-22 12:21 作者: 不規(guī)則的跳動(dòng) 時(shí)間: 2025-3-22 14:52 作者: incontinence 時(shí)間: 2025-3-22 19:20 作者: 宿醉 時(shí)間: 2025-3-22 21:46 作者: Trabeculoplasty 時(shí)間: 2025-3-23 01:50
Nathaniel Shank,Kara M. George Rosenker,Ethan A. Englund,Andrew V. Dix,Elizabeth E. Rastede,Daniel H作者: Interregnum 時(shí)間: 2025-3-23 07:05
Maryam Imani Nejad,Xu Guo,Kurt Housh,Christopher Nel,Zhiyu Yang,Nathan E. Price,Yinsheng Wang,Kent S作者: municipality 時(shí)間: 2025-3-23 10:55 作者: conservative 時(shí)間: 2025-3-23 14:29 作者: JUST 時(shí)間: 2025-3-23 19:50 作者: decipher 時(shí)間: 2025-3-24 02:13 作者: 難管 時(shí)間: 2025-3-24 06:02 作者: Recessive 時(shí)間: 2025-3-24 09:38 作者: 滑動(dòng) 時(shí)間: 2025-3-24 10:49 作者: 注意到 時(shí)間: 2025-3-24 16:40 作者: 畢業(yè)典禮 時(shí)間: 2025-3-24 22:31
,Synthesis and Application of LKγT Peptide Nucleic Acids,th high precision and accuracy provides a distinct advantage over other ligand-display systems, such as polymers, because the number and position of the ligand(s) can be accurately and fully characterized. Here we describe the synthesis of peptide nucleic acids (PNAs), which are oligonucleotide mimi作者: DEVIL 時(shí)間: 2025-3-25 02:27
Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids,obes and medicinally relevant compounds. Simple synthetic procedures designed to easily and quickly manipulate amino sugar (neomycin, kanamycin) to more powerful and selective ligands are presented in this chapter. We describe representative procedures for (a) aminoglycoside conjugation and (b) prel作者: 不透明 時(shí)間: 2025-3-25 03:26 作者: 軍火 時(shí)間: 2025-3-25 10:29
Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-M commonly incorporated into the DNA for subsequent modification by click reaction. However, it is partially oxidized during deprotection during solid-phase synthesis, resulting in a ketone that is no longer accessible for click modification. To enable the high-fidelity solid-phase synthesis of EdU-c作者: Amnesty 時(shí)間: 2025-3-25 14:12 作者: 可耕種 時(shí)間: 2025-3-25 17:02
Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet,fication of DNA with the four-letter genetic alphabet. Here, we describe PCR amplification with a six-letter alphabet that includes the two natural dA-dT and dG-dC base pairs and an unnatural base pair (UBP) formed between the synthetic nucleotides d. and d. or d. or analogs of these synthetic nucle作者: Bureaucracy 時(shí)間: 2025-3-25 22:15
Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis,alth. This chapter describes the application of an artificial nucleoside/nucleotide system that functions as a biochemical probe to quantify TLS activity under in vitro and in vivo conditions. For in vitro studies, the artificial nucleotide, 3-ethynyl-5-nitroindolyl-2′-deoxyriboside triphosphate (3-作者: inveigh 時(shí)間: 2025-3-26 02:55 作者: 雄偉 時(shí)間: 2025-3-26 07:46 作者: 寵愛 時(shí)間: 2025-3-26 10:18 作者: 外形 時(shí)間: 2025-3-26 14:01
Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation,n. Monitoring kinetic aspects of this catalytic process provides mechanistic information regarding polymerase-mediated DNA synthesis and the influences of nucleobase structure. For example, a range of polymerases have different capacities to synthesize DNA depending on the structure of the inserted 作者: Conserve 時(shí)間: 2025-3-26 17:31 作者: NATAL 時(shí)間: 2025-3-26 20:58 作者: BLINK 時(shí)間: 2025-3-27 04:59
1064-3745 expertsThis volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant作者: 收養(yǎng) 時(shí)間: 2025-3-27 07:43
Synthesis of Nucleobase-Functionalized Morpholino Monomers,genations of the nucleobases in suitable position and subsequent Pd-catalyzed Sonogashira and Suzuki reactions. The sequential synthetic steps are described in detail in this chapter, and the examples are shown in tables.作者: 四指套 時(shí)間: 2025-3-27 11:47
Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Montaining DNA, this protocol describes a procedure to perform the click reaction on the solid phase before deprotection. Afterwards, the DNA can be deprotected and purified according to standard procedures, and the full modification of EdU with the azide of choice can be analyzed by HPLC and HPLC/MS.作者: savage 時(shí)間: 2025-3-27 17:30
Book 2019ligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs.? Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, li作者: Inscrutable 時(shí)間: 2025-3-27 19:53 作者: 全國性 時(shí)間: 2025-3-28 00:01 作者: 的闡明 時(shí)間: 2025-3-28 02:38
,2′-,,4′-,-Ethyleneoxy-Bridged 2′-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesiites into oligonucleotides, (3) UV-melting experiments of the duplexes of the modified oligonucleotides and complementary RNA, and (4) nuclease degradation experiments of the modified oligonucleotides.作者: flaggy 時(shí)間: 2025-3-28 08:28 作者: 聰明 時(shí)間: 2025-3-28 11:27 作者: 膠狀 時(shí)間: 2025-3-28 15:04 作者: 松馳 時(shí)間: 2025-3-28 18:55 作者: Stricture 時(shí)間: 2025-3-29 02:23 作者: linguistics 時(shí)間: 2025-3-29 06:29
FRET Assay for Ligands Targeting the Bacterial A-Site RNA,omitant restoration of the donor’s emission. Plotting the relative emission intensity of both the donor and acceptor as a function of ligand concentration followed by fitting of the data points to a dose-response curve yields IC. values, one possible measure of the antibiotic potency of new A-site binders.作者: Neuralgia 時(shí)間: 2025-3-29 08:14 作者: 商品 時(shí)間: 2025-3-29 12:49 作者: 產(chǎn)生 時(shí)間: 2025-3-29 18:03
978-1-4939-9218-8Springer Science+Business Media, LLC, part of Springer Nature 2019作者: MURKY 時(shí)間: 2025-3-29 23:02 作者: 不規(guī)則 時(shí)間: 2025-3-30 03:30 作者: 猜忌 時(shí)間: 2025-3-30 07:07 作者: 構(gòu)成 時(shí)間: 2025-3-30 09:29 作者: Gum-Disease 時(shí)間: 2025-3-30 14:23
Flexible Nucleic Acids (FNAs) as Informational Molecules: Enzymatic Polymerization of fNTPs on DNA The methodology enabling enzymatic and nonenzymatic information transfer with FNAs is described. This methodology includes the chemical synthesis of fNTPs and fN phosphoramidites, in addition to protocols for the enzymatic and nonenzymatic transfer of information.作者: Engaged 時(shí)間: 2025-3-30 19:18
Nathaniel ShankIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 帽子 時(shí)間: 2025-3-30 21:37
Foreign Exchange Rate Determination, be understood as a driver for social evolution. The elements and structures of governance are contingent and in constant transformation. This transformation is driven by the productive tensions between discourses, with the results of each operation serving as input for the next one.作者: 天賦 時(shí)間: 2025-3-31 01:22
Die gonadotrope Funktion des Hypophysenvorderlappens. Gehalt der Hypophyse an gonadotropem Hormon,mu?te ich daraus schlie?en, da? hier ein biologisches Gesetz vorliegt, da? n?mlich das in den Follikelzellen gebildete Hormon auf den Follikel selbst und das Ei ohne jeden Einflu? ist. Der Impuls für die Ovarialfunktion mu?te also an anderer Stelle gesucht werden.作者: obsolete 時(shí)間: 2025-3-31 06:12 作者: 設(shè)施 時(shí)間: 2025-3-31 11:04 作者: giggle 時(shí)間: 2025-3-31 13:25 作者: fledged 時(shí)間: 2025-3-31 17:58
The Analogies of the Soul: Brentano, Aristotle and the Project of a Scientific Psychologyutiny of the 1867 treatise, .. The recourse to Aristotle remains necessary for understanding Brentano’s project, to wit, an empirical psychology that does not ultimately abandon the metaphysical framework from whence it originates.作者: TEN 時(shí)間: 2025-3-31 23:19
commitment to treating talk of propositions—as the things towards which our beliefs are attitudes—as talk of entities that actually exist and that play a constitutive and explanatory role in the activity of th978-981-16-3519-9978-981-16-3517-5作者: xanthelasma 時(shí)間: 2025-4-1 05:17 作者: 啟發(fā) 時(shí)間: 2025-4-1 07:53 作者: 捕鯨魚叉 時(shí)間: 2025-4-1 13:32
