標(biāo)題: Titlebook: Nitric Oxide; Methods and Protocol Alexander Mengel,Christian Lindermayr Book 2018 Springer Science+Business Media, LLC, part of Springer N [打印本頁] 作者: hexagon 時(shí)間: 2025-3-21 16:46
書目名稱Nitric Oxide影響因子(影響力)
書目名稱Nitric Oxide影響因子(影響力)學(xué)科排名
書目名稱Nitric Oxide網(wǎng)絡(luò)公開度
書目名稱Nitric Oxide網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Nitric Oxide被引頻次
書目名稱Nitric Oxide被引頻次學(xué)科排名
書目名稱Nitric Oxide年度引用
書目名稱Nitric Oxide年度引用學(xué)科排名
書目名稱Nitric Oxide讀者反饋
書目名稱Nitric Oxide讀者反饋學(xué)科排名
作者: 健談 時(shí)間: 2025-3-21 23:41
Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrodear [NO] measurement utilizing an L-shaped amperometric integrated NO-selective electrode. This method not only provides an elegant and convenient approach to real-time the measurement of NO in physiological environments but also mimics the loss of NO caused by rapid NO diffusion combined with its reactivity in the biological milieu.作者: 情感 時(shí)間: 2025-3-22 03:57
Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Mollusksxclusively associated to an enzyme family known as Nitric Oxide Synthases (NOSs). The digestive glands of mollusks are a major target for oxidative disruption related to environmental stress. A simple EPR-methodology to asses both, the presence of NO and its rate of generation in tissues from different mollusk species, is reported here.作者: stress-response 時(shí)間: 2025-3-22 07:59
Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Techniquen made possible by the development of the Biotin-Switch Technique (BST) in the early 2000s. Here, we describe the BST protocol we routinely use to check in vitro S-nitrosylation of recombinant proteins induced by NO donors.作者: HOWL 時(shí)間: 2025-3-22 10:51
A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems: Bell Pepper Fruits as a Models. In this chapter a method to treat plant materials with NO is provided using bell pepper fruits as a model. This method is cheap, free of side effects, and easy to apply since it only requires common chemicals and tools available in any biology laboratory.作者: dialect 時(shí)間: 2025-3-22 13:14
Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps NO. signals. Here, we describe detailed methods suitable for imaging of NO. signals in mammalian cells. This novel approach may facilitate a broad range of studies to (re)investigate the complex NO. biochemistry in living cells.作者: 享樂主義者 時(shí)間: 2025-3-22 19:02
Direct Measurement of S-Nitrosothiols with an Orbitrap Fusion Mass Spectrometer: S-Nitrosoglutathionrapid assay to estimate protein SNO abundance with intact protein electrospray ionization mass spectrometry. Originally designed using recombinant GSNOR, these methods are applicable to any purified protein to test for or further study nitrosatable cysteines.作者: 細(xì)菌等 時(shí)間: 2025-3-22 21:35 作者: 珊瑚 時(shí)間: 2025-3-23 04:47
1064-3745 ation advice from the experts.This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part on作者: Customary 時(shí)間: 2025-3-23 05:35 作者: assent 時(shí)間: 2025-3-23 12:48
A Proteomics Workflow for Dual Labeling Biotin Switch Assay to Detect and Quantify Protein S-Nitroylrage using a single tag. Here, we present a parallel dual labeling strategy followed by an optimized proteomics workflow, which maximizes the overall detection of SNO by reducing the labeling bias derived from the use of a single tag-capture approach.作者: 廣口瓶 時(shí)間: 2025-3-23 15:33
Identification of Tyrosine and Nitrotyrosine with a Mixed-Mode Solid-Phase Extraction Cleanup Followpreparation assay based on mixed-mode solid-phase extraction (SPE) which was developed for the quantification of trace NO.Tyr in . extracts by liquid chromatography–electrospray time-of-flight mass spectrometry (LC-TOFMS).作者: Contort 時(shí)間: 2025-3-23 18:19
Detection of Nitric Oxide by Membrane Inlet Mass Spectrometryies from the bulk sample into the mass spectrometer. Silastic tubing allows the introduction of small gaseous molecules including nitric oxide (NO) into the high vacuum of a mass spectrometer. We describe the measurement of NO generated chemically from nitrite and MAHMA NONOate as well as enzymatically by nitric oxide synthase (NOS).作者: LUT 時(shí)間: 2025-3-24 00:52
Surface Plasmon Resonance Spectroscopy for Detection of ,-Nitrosylated Proteins describe a surface plasmon resonance (SPR) spectroscopy method for detecting .nitrosylated proteins. This method can be applied for the indirect determination of .nitrosylated proteins in biological samples.作者: 廣口瓶 時(shí)間: 2025-3-24 04:55 作者: miracle 時(shí)間: 2025-3-24 08:08
José M. Palma,Carmelo Ruiz,Francisco J. Corpasllenging similarity Galileo perceived between what he held to be true concerning pendulum motion on the one hand and accelerated motion on inclined planes on the other hand, resulted in an intense phase of work in which he inquired deeply into the relation of swinging and rolling. Galileo did not ac作者: 一小塊 時(shí)間: 2025-3-24 11:32 作者: conception 時(shí)間: 2025-3-24 15:41
Emrah Eroglu,Helmut Bischof,Suphachai Charoensin,Markus Waldeck-Weiermaier,Wolfgang F. Graier,Roland should ever the closer approach the time to fall along the arc, which he believed was the same as the time to swing along the same arc, the closer the actual path of motion considered approximated the arc. With this understanding, Galileo returned to the measurement data of an experiment in which h作者: Incorporate 時(shí)間: 2025-3-24 19:51
Heaseung Sophia Chung,Christopher I. Murray,Jennifer E. Van Eyknd or to appreciate the Swiss contribution to international banking during recent years without first considering its past and its background. While it might seem odd that a non-Swiss economist should even consider researching and writing on the subject of Swiss banking history and theory, there is 作者: Fillet,Filet 時(shí)間: 2025-3-25 02:11
Lili Zhang,Pengpeng Shang,Changbao Chen,Jie Zhou,Shuhua Zhund or to appreciate the Swiss contribution to international banking during recent years without first considering its past and its background. While it might seem odd that a non-Swiss economist should even consider researching and writing on the subject of Swiss banking history and theory, there is 作者: 從容 時(shí)間: 2025-3-25 05:05
Dimitrios Tsikas,Erik Hanffnd or to appreciate the Swiss contribution to international banking during recent years without first considering its past and its background. While it might seem odd that a non-Swiss economist should even consider researching and writing on the subject of Swiss banking history and theory, there is 作者: 北極人 時(shí)間: 2025-3-25 09:02
Sébastien Aimé,Siham Hichami,David Wendehenne,Olivier Lamottend or to appreciate the Swiss contribution to international banking during recent years without first considering its past and its background. While it might seem odd that a non-Swiss economist should even consider researching and writing on the subject of Swiss banking history and theory, there is 作者: BILIO 時(shí)間: 2025-3-25 15:35 作者: Cryptic 時(shí)間: 2025-3-25 19:04 作者: 狼群 時(shí)間: 2025-3-25 22:28
Azam Shekariesfahlan,Christian Lindermayrof the First and Second World Wars, Switzerland survived as a successfully neutral and independent small nation in the heart of war-torn Europe. At the end of this century its inhabitants are enjoying one of the highest living standards among industrialised countries. Switzerland lacks natural resou作者: 立即 時(shí)間: 2025-3-26 02:09
Adil Hussain,Byung-Wook Yun,Gary J. Loakeof the First and Second World Wars, Switzerland survived as a successfully neutral and independent small nation in the heart of wartorn Europe. At the beginning of this century its inhabitants are enjoying one of the highest living standards among industrialised countries. Switzerland lacks natural 作者: NEX 時(shí)間: 2025-3-26 05:00
A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems: Bell Pepper Fruits as a Modelnt, senescence, and fruit ripening. In the latter physiological process, NO has been reported to play an opposite role to ethylene. Thus, treatment of fruits with NO may lead to delay ripening independently of whether they are climacteric or nonclimacteric. In many cases different methods have been 作者: vitrectomy 時(shí)間: 2025-3-26 10:58
Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrodeer knowledge and develop treatment plans in certain disorders. The direct quantitative measurement of [NO] in situ in an intact cellular complex should be useful in tracking real-time and rapid changes at nanomolar levels. In this work, we describe the direct, real-time, and quantitative intracellul作者: enumaerate 時(shí)間: 2025-3-26 15:43 作者: 可憎 時(shí)間: 2025-3-26 19:20
Detection of Nitric Oxide by Membrane Inlet Mass Spectrometryit of detection of 10?nM and a linear response to 50?μM. MIMS utilizes a semipermeable membrane to partition analytes based on physicochemical properties from the bulk sample into the mass spectrometer. Silastic tubing allows the introduction of small gaseous molecules including nitric oxide (NO) in作者: Kinetic 時(shí)間: 2025-3-26 23:26 作者: alabaster 時(shí)間: 2025-3-27 04:38 作者: ADAGE 時(shí)間: 2025-3-27 07:24
Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complemnaling. The biotin switch technique for specifically labeling S-nitrosylated proteins opened the way to proteomic identification of these modifications. Since then, several variations and adaptations of the original method have been applied..We describe here the protocols of several techniques that 作者: TAG 時(shí)間: 2025-3-27 09:49 作者: 透明 時(shí)間: 2025-3-27 15:12
Surface Plasmon Resonance Spectroscopy for Detection of ,-Nitrosylated Proteins The biotin-switch technique (BST) has become a mainstay method for detection of .nitrosylated proteins in biological samples. On the basis of BST, we describe a surface plasmon resonance (SPR) spectroscopy method for detecting .nitrosylated proteins. This method can be applied for the indirect dete作者: palliate 時(shí)間: 2025-3-27 18:31
Measurement of , -Nitrosoglutathione in Plasma by Liquid Chromatography–Tandem Mass Spectrometry.-nitrosoglutathione (GSNO) in human plasma. .-[.N]Nitrosoglutathione (GS.NO) serves as the internal standard. The protocol involves inactivation of plasma γ-glutamyltransferase activity by serine-borate, stabilization of GSNO with EDTA, and avoidance of .-transnitrosylation reactions by blocking SH作者: 庇護(hù) 時(shí)間: 2025-3-27 23:31
Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Techniqueanslational modifications of proteins through its direct or indirect reaction with specific amino acid residues. Among them, S-nitrosylation has been shown to be involved in a broad range of cellular signaling pathways both in animals and plants. The identification of S-nitrosylated proteins has bee作者: 輕而薄 時(shí)間: 2025-3-28 06:11 作者: Canyon 時(shí)間: 2025-3-28 10:07
Identification of Tyrosine and Nitrotyrosine with a Mixed-Mode Solid-Phase Extraction Cleanup Followoteins. Different methods have been developed for identifying nitrotyrosine in biological samples. However, these analyses are difficult because tyrosine nitration is a very low-abundance posttranslational protein modification (PTM) and the lack of efficient enrichment methods for detection. The ide作者: 性上癮 時(shí)間: 2025-3-28 12:04
Electrophoretic Detection and Confocal Microscopic Imaging of Tyrosine Nitrated Proteins in Plant Tiscopy (CLSM) imaging. One-dimensional polyacrylamide gel electrophoresis (1D PAGE) followed by Western blotting using polyclonal antibody against 3-nitrotyrosine residues enables detection of tyrosine nitrated proteins in plant cells. Here we describe detection of tyrosine nitrated proteins in the h作者: 騙子 時(shí)間: 2025-3-28 18:06 作者: liposuction 時(shí)間: 2025-3-28 18:50 作者: 不要嚴(yán)酷 時(shí)間: 2025-3-28 23:44
Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiolsis involved in vasodilation and neurotransmission and is present in exhaled breath. In plants, it regulates both plant immune function and numerous developmental programs. The high reactivity and short half-life of NO and cross-reactivity of its various derivatives make its quantification difficult.作者: 表示向下 時(shí)間: 2025-3-29 04:55 作者: Entrancing 時(shí)間: 2025-3-29 08:17 作者: 不安 時(shí)間: 2025-3-29 11:31
Electrophoretic Detection and Confocal Microscopic Imaging of Tyrosine Nitrated Proteins in Plant Tiick wax sections of tissue followed by incubation in primary anti-nitrotyrosine antibody (dilution 1:200) and secondary Cy-3 labeled anti-rabbit IgG antibody (dilution 1:1500). Confocal laser scanning microscopy analysis is undertaken using argon lasers (ex: 530–550 nm and em: 570?nm) at pinhole 1. 作者: conduct 時(shí)間: 2025-3-29 18:57 作者: ABYSS 時(shí)間: 2025-3-29 21:19 作者: 喪失 時(shí)間: 2025-3-29 23:55 作者: Acquired 時(shí)間: 2025-3-30 05:47
1064-3745 tative, .Nitric Oxide: Methods and Protocols. is a valuable resource for anyone interested in learning more about this evolving field..978-1-4939-9260-7978-1-4939-7695-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: FAZE 時(shí)間: 2025-3-30 10:17 作者: cliche 時(shí)間: 2025-3-30 14:14 作者: 廢止 時(shí)間: 2025-3-30 17:13
Alicia Izquierdo-álvarez,Daniel Tello,J. Daniel Cabrera-García,Antonio Martínez-Ruiz作者: 冰雹 時(shí)間: 2025-3-30 21:41
Mounira Chaki,Beatriz Sánchez-Calvo,Alfonso Carreras,Raquel Valderrama,Juan C. Begara-Morales,Franci作者: 鋼筆尖 時(shí)間: 2025-3-31 02:11 作者: 使苦惱 時(shí)間: 2025-3-31 05:08
José M. Palma,Carmelo Ruiz,Francisco J. Corpasble science able to meet the standards of the time. However, his attempt to found the new science on arguments starting from dynamical assumptions failed. In 1604 Galileo started an afresh attack on the problem of the foundation, availing himself of a new approach which perceived naturally accelerat作者: Customary 時(shí)間: 2025-3-31 09:33
Sana N. Khan,Roohi Jeelani,Husam M. Abu-Soudble science able to meet the standards of the time. However, his attempt to found the new science on arguments starting from dynamical assumptions failed. In 1604 Galileo started an afresh attack on the problem of the foundation, availing himself of a new approach which perceived naturally accelerat作者: MARS 時(shí)間: 2025-3-31 17:16
Emrah Eroglu,Helmut Bischof,Suphachai Charoensin,Markus Waldeck-Weiermaier,Wolfgang F. Graier,Rolande pendulum as Galileo would have expected but differed from it by about 20%. Galileo’s considerations had, moreover, yielded strong indications that this difference would not vanish if polygonal paths composed of an even higher number of sides were considered. The difference finds its explanation in作者: RECUR 時(shí)間: 2025-3-31 18:29
Heaseung Sophia Chung,Christopher I. Murray,Jennifer E. Van Eyknot have. They lack, in a word, the objectivity of the foreigner. They also lack the theoretical framework within which Swiss banking may be compared with other systems. It is, after all, only by such comparison and contrast that the Swiss banking achievements may be appreciated.
