標題: Titlebook: Next Generation Sequencing; Methods and Protocol Steven R. Head,Phillip Ordoukhanian,Daniel R. Salo Book 2018 Springer Science+Business Med [打印本頁] 作者: 不能平庸 時間: 2025-3-21 17:34
書目名稱Next Generation Sequencing影響因子(影響力)
作者: 重畫只能放棄 時間: 2025-3-21 22:19 作者: Custodian 時間: 2025-3-22 02:13
ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Ctrates how robust, bio-orthogonal chemistry can be harnessed in vitro to capture and dissect complex biological processes. Here, we describe an updated protocol for the synthesis of “ClickSeq” libraries.作者: 易怒 時間: 2025-3-22 06:33
A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures,opment of a means of estimating the cell type composition of blood samples from their bulk RNA-Seq profiles. We also illustrate the importance of adjusting for the potential confounding effect of cellular heterogeneity in the context of statistical inference in a whole blood RNA-Seq dataset.作者: Esophagus 時間: 2025-3-22 10:50
Next-Generation Sequencing of Genome-Wide CRISPR Screens,ens using high-throughput next-generation sequencing (NGS) allows for the adoption of the technology in a variety of laboratories interested in diverse biologic questions. Here, we describe the protocol to generate next-generation sequencing libraries from genome-wide CRISPR genomic screens.作者: 憎惡 時間: 2025-3-22 13:03
An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome,ing purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with these two approaches can generate a comprehensive picture on how translational regulation determines protein output.作者: jettison 時間: 2025-3-22 17:27
Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis,hole genome amplification (WGA) on isolated blastomere(s), up to data analysis for CNA detection. The process is described generically and can also be used to perform CNA analysis on a limited number of cells (down to a single cell) in other applications. This unique description also includes some tips and tricks to increase the chance of success.作者: EVEN 時間: 2025-3-22 23:46 作者: 不公開 時間: 2025-3-23 04:51
Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Dg, nascent RNA sequencing, and other protocols where polymerase termination sites need to be profiled. The utilization of solid-phase bead chemistries facilitates simple workflow and efficient library yields.作者: 屈尊 時間: 2025-3-23 08:06
Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Apprpre-amplified with concomitant integration of the sequencing adapters, and libraries are subsequently amplified and indexed by PCR. Using scBS-Seq we can accurately measure DNA methylation at up to 50% of individual CpG sites and 70% of CpG islands.作者: emission 時間: 2025-3-23 12:30
1064-3745 ation advice from the experts.This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic 作者: 打折 時間: 2025-3-23 17:48
ous courses available at that time did not fully serve their needs. While a few large companies had developed in-house training arrangements, the many medium and smaller fi.rms in the raw material supply, paint manufacturing or paint user industries, were unable to provide their own comprehensive tr作者: defray 時間: 2025-3-23 20:54
Lieselot Deleye,Dieter De Coninck,Dieter Deforce,Filip Van Nieuwerburghcharged polymers migrate electrophoretically to the electrode (workpiece) of opposite charge. At the electrode the polymers become insoluble and form an insulating film. Technical development based on this phenomenon began early in this century. In 1919 a patent. was granted for painting of conducti作者: Nostalgia 時間: 2025-3-24 00:26 作者: maculated 時間: 2025-3-24 04:28 作者: evasive 時間: 2025-3-24 10:14
Elizabeth Jaworski,Andrew Routhcreasingly important and prevalent activity, is the primary topic of this book. Our goals are to outline the fundamentals of surface computing (a still maturing technology), review relevant work on collaborative data analysis, describe frameworks for understanding collaborative processes, and provid作者: 珊瑚 時間: 2025-3-24 11:52
Heather J. Lee,Sébastien A. Smallwood important and prevalent activity, is the primary topic of this book. Our goals are to outline the fundamentals of surface computing (a still maturing technology), review relevant work on collaborative data analysis, describe frameworks for understanding collaborative processes, and provide a better作者: 無法破譯 時間: 2025-3-24 15:32
Robert M. Bowers,Janey Lee,Tanja Woykecreasingly important and prevalent activity, is the primary topic of this book. Our goals are to outline the fundamentals of surface computing (a still maturing technology), review relevant work on collaborative data analysis, describe frameworks for understanding collaborative processes, and provid作者: Type-1-Diabetes 時間: 2025-3-24 19:17
Phillip A. Morin,Andrew D. Foote,Christopher M. Hill,Benoit Simon-Bouhet,Aimee R. Lang,Marie Louis Control held in Washington, D.C., September 10-13, 1978. This Symposium was a part of the 4th International Symposium on Contamination Control held under the auspices of the International Committee of Contamination Control Societies, and the Institute of Environmental Sciences (U.S.A.) was the offi作者: 專心 時間: 2025-3-24 23:40
Sabrina Shore,Jordana M. Henderson,Anton P. McCaffreyeld in Washington, D.C., September 10-13, 1978. This Symposium was a part of the 4th International Symposium on Contamination Control held under the auspices of the International Committee of Contamination Control Societies, and the Institute of Environmental Sciences (U.S.A.) was the official host.作者: Resection 時間: 2025-3-25 03:46 作者: 彩色 時間: 2025-3-25 08:19 作者: 透明 時間: 2025-3-25 11:55
Edwin H. Yau,Tariq M. Ranarations at surfaces: yet the number is not so great that cataloguing these structures is too daunting a task. We felt that now was the right moment to begin a compilation that could be updated at frequent intervals to give a comprehensive picture of the known surface world. The following pages are t作者: irreducible 時間: 2025-3-25 18:53 作者: 放棄 時間: 2025-3-25 22:18 作者: 牛的細微差別 時間: 2025-3-26 00:42
tion-induced amorphization, crystallization, and various microstructural developments arising from defect production and annealing, the book delves into controlled modifications of physical and chemical properties, micro and nano-structuring of surfaces, and the creation and behavior of structures f作者: 博愛家 時間: 2025-3-26 06:02
An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome, functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with作者: FLIRT 時間: 2025-3-26 08:33 作者: 粗糙 時間: 2025-3-26 16:03 作者: 改革運動 時間: 2025-3-26 19:46
Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedur nanotechnology to generate extra-long reads from libraries prepared using only standard molecular biology lab equipment. The flexibility and the portability of the platform makes it ideal for point-of-interest and real-time surveillance applications. However, MinION’s limited capacity is not enough作者: occurrence 時間: 2025-3-26 21:02
Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing,techniques 58:33–36, 2015). At its core is the principle of using gene-specific primers (GSPs) to “seed” (or target) the reaction and universal primers to “drive” the majority of the reaction. In this manner, effects on amplification efficiencies across the target amplicons can, to a large extent, b作者: 轉(zhuǎn)換 時間: 2025-3-27 04:35
ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Cick-chemistry in place of enzymes for the ligation of sequencing adaptors. We found that this approach dramatically reduces artifactual chimera formation, allowing the study of rare recombination events that include viral replication intermediates and defective-interfering viral RNAs. ClickSeq illus作者: CRUDE 時間: 2025-3-27 05:55
Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Apprlfite-based approaches, it is possible to obtain comprehensive genome-wide maps of the mammalian DNA methylation landscape with a single-nucleotide resolution and absolute quantification. However, these methods were only applicable to bulk populations of cells. Here, we present a protocol to perform作者: definition 時間: 2025-3-27 09:48 作者: 一再煩擾 時間: 2025-3-27 13:57 作者: 解脫 時間: 2025-3-27 21:36 作者: 用手捏 時間: 2025-3-28 00:26
Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral tudy of as-yet uncultured microbes and viruses. Studies of complex microbial communities require high-quality data to generate valid results. Here, we detail current methods of microbial and viral community sample acquisition, DNA extraction, sample preparation, and sequencing on Illumina high-throu作者: Watemelon 時間: 2025-3-28 05:51
A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures,terogeneous tissues, cell admixtures with relative proportions that can vary several fold across samples, poses a significant challenge. Blood is perhaps the most egregious example. Here, we describe in detail a computational pipeline for RNA-Seq data preparation and statistical analysis, with devel作者: Arbitrary 時間: 2025-3-28 09:58
Next-Generation Sequencing of Genome-Wide CRISPR Screens,a powerful tool for systematic genomic perturbation in mammalian cells and provide an alternative to previous screens utilizing RNA interference technology. The wide availability of these libraries through public plasmid repositories as well as the decreasing cost and speed in quantifying these scre作者: 領(lǐng)袖氣質(zhì) 時間: 2025-3-28 11:30 作者: 放逐 時間: 2025-3-28 14:50 作者: genesis 時間: 2025-3-28 20:54
the Sydney Technical College. The scope of the course was designed to cover all aspects of surface coatings technology ranging from raw materials and formulations to the production, testing, evaluation, application and use of finished products. The course proved to be highly successful and in 1973 a similar s978-94-011-6942-4978-94-011-6940-0作者: climax 時間: 2025-3-28 23:04 作者: 某人 時間: 2025-3-29 04:51 作者: 膝蓋 時間: 2025-3-29 10:29 作者: Paraplegia 時間: 2025-3-29 15:12 作者: 潰爛 時間: 2025-3-29 17:18
Phillip A. Morin,Andrew D. Foote,Christopher M. Hill,Benoit Simon-Bouhet,Aimee R. Lang,Marie Louisways been a bete noire. In short, people dealing with surfaces are afflicted with molysmophobiat, and rightfully so. In the past, the subject of surface contamination had been discussed in various meetings, but this symposium was hailed as the most comprehensive symposium ever held on this important topic, as978-1-4684-3508-5978-1-4684-3506-1作者: 長處 時間: 2025-3-29 22:43
Sabrina Shore,Jordana M. Henderson,Anton P. McCaffreya bete noire. In short, people dealing with surfaces are afflicted with molysmophobiat, and rightfully so. In the past, the subject of surface contamination had been discussed in various meetings, but this symposium was hailed as the most comprehensive symposium ever held on this important topic, as作者: 絆住 時間: 2025-3-30 00:24
Pedro J. Torres,Scott T. Kelley Ph.D.a bete noire. In short, people dealing with surfaces are afflicted with molysmophobiat, and rightfully so. In the past, the subject of surface contamination had been discussed in various meetings, but this symposium was hailed as the most comprehensive symposium ever held on this important topic, as作者: TOM 時間: 2025-3-30 07:20
Casey P. Shannon,Chen Xi Yang,Scott J. Tebbuttno doubt have its critics, and in subsequent editions may well be improved, but it has been arrived at after extensive consultation. A problem that we faced in putting structures into standard format was the diversity of conventions used in the literature. It is to be hoped that our system will have sufficien978-90-277-2554-7978-94-009-3935-6作者: FRONT 時間: 2025-3-30 08:41
Edwin H. Yau,Tariq M. Ranaave its critics, and in subsequent editions may well be improved, but it has been arrived at after extensive consultation. A problem that we faced in putting structures into standard format was the diversity of conventions used in the literature. It is to be hoped that our system will have sufficien作者: Arresting 時間: 2025-3-30 15:54 作者: Flatter 時間: 2025-3-30 17:04
Phillip Ordoukhanian,Jessica Nichols,Steven R. HeadEnergy Electron Diffraction (LEED): this technique has now determined the atomic positions for nearly a hundred surfaces, whether in the clean state or with additional foreign atoms or molecules. The main aim of this book is to publish a set of computer pro- grams that has been specifically designed作者: 膝蓋 時間: 2025-3-30 22:11
licon energy and semiconductor electronics, providing practical knowledge for professionals engaged in these fields. Additionally, the work is of significance to a broad spectrum of specialists and managers in various organizations involved in the development of lithium-ion batteries for energy stor作者: 少量 時間: 2025-3-31 02:56 作者: gout109 時間: 2025-3-31 07:33
Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing,orithms (Pope et al., Source Code Biol Med 9:3, 2014; Park et al., BMC Bioinformatics 17:165, 2016). Hi-Plex offers a streamlined workflow that is suitable for testing large numbers of specimens without the need for automation.
