標題: Titlebook: Next Generation Culture Platforms for Reliable In Vitro Models; Methods and Protocol Tiziana A.L. Brevini,Alireza Fazeli,Kursad Turksen Boo [打印本頁] 作者: FERAL 時間: 2025-3-21 17:55
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models影響因子(影響力)
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models影響因子(影響力)學科排名
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models網絡公開度
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models網絡公開度學科排名
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models被引頻次
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models被引頻次學科排名
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models年度引用
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models年度引用學科排名
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models讀者反饋
書目名稱Next Generation Culture Platforms for Reliable In Vitro Models讀者反饋學科排名
作者: 有害 時間: 2025-3-21 23:30
978-1-0716-1248-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: REP 時間: 2025-3-22 02:35
Next Generation Culture Platforms for Reliable In Vitro Models978-1-0716-1246-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 痛苦一下 時間: 2025-3-22 06:46 作者: Intercept 時間: 2025-3-22 12:07
https://doi.org/10.1007/978-1-0716-1246-0Cell culture; 3D culture; Exosomes; Organ development; Drug testing; Functional scaffolds; Nanoparticles作者: ABYSS 時間: 2025-3-22 16:42
Tiziana A.L. Brevini,Alireza Fazeli,Kursad TurksenIncludes cutting-edge techniques.Provides step-by-step details essential for reproducible results.Contains key implementation advice from the experts作者: 故意釣到白楊 時間: 2025-3-22 20:22
to simulation results from the Vensim program, whose main pr.Supply Chain Simulation. allows readers to practice modeling and simulating a multi-level supply chain. The chapters are a combination of the practical and the theoretical, covering:.knowledge of simulation methods and techniques,.the conc作者: Vaginismus 時間: 2025-3-22 21:27
Alberto Maria Luciano,Rodrigo Garcia Barros,Ana Caroline Silva Soares,Jose Buratini,Valentina Lodde,to simulation results from the Vensim program, whose main pr.Supply Chain Simulation. allows readers to practice modeling and simulating a multi-level supply chain. The chapters are a combination of the practical and the theoretical, covering:.knowledge of simulation methods and techniques,.the conc作者: invulnerable 時間: 2025-3-23 04:13 作者: amenity 時間: 2025-3-23 09:17 作者: 軌道 時間: 2025-3-23 10:14
Antonio Murillo,Marta Mu?ozichen S?ulen Prozesse, Information und Softwareunterstützung. Das Ziel s?mtlicher Aktivit?ten ist die Bereitstellung und Verarbeitung der für die unternehmensübergreifende Planung und Steuerung ben?tigten Informationen. Daraus ergeben sich für die Implementierung des SCM die folgenden Handlungsfelde作者: amygdala 時間: 2025-3-23 16:36
Matteo Ghiringhelli,Yousef Abboud,Snizhanna V. Chorna,Irit Huber,Gil Arbel,Amira Gepstein,Georgia Pegegangen, erarbeitet und in gro?en Teilen ausgesch?pft worden sind, gilt es nun unternehmensübergreifenden Verbesserungen zu erzielen. Diese weltweite Entwicklung im Supply Network führt auch zu neuen Herausforderungen für die Logistik. Durch Leistungsverlagerungen auf die Partner des Supply Network作者: Arb853 時間: 2025-3-23 19:12 作者: Formidable 時間: 2025-3-24 00:25 作者: 魔鬼在游行 時間: 2025-3-24 02:28 作者: 浪費時間 時間: 2025-3-24 09:49 作者: atopic 時間: 2025-3-24 13:04 作者: Osteons 時間: 2025-3-24 16:13
Keerthie Dissanayake,Kasun Godakumara,Alireza Fazeli of the nearly one billion households with purchasing power will live in these economies. This scenario not only provides opportunities for multinationals, it also creates a sense of urgency. Unilever, GE, and P&G, among others, already conduct a large percent of their R&D and business transactions 作者: recession 時間: 2025-3-24 19:06 作者: Gene408 時間: 2025-3-25 01:39
Keerthie Dissanayake,Getnet Midekessa,Freddy L?ttekivi,Alireza Fazelie advantage. While value creation is shaped by external drivers such as market volatility, technology, product and service offering and disruption, it can be stymied by the internal stresses arising from the need to minimize costs, limitations in process redesign, waste minimization and the unavaila作者: ABOUT 時間: 2025-3-25 05:45
Karina Ca?ón-Beltrán,Meriem Hamdi,Rosane Mazzarella,Yulia N. Cajas,Claudia L. V. Leal,Alfonso Gutiér examples useful for benchmarking.Today, supply chain transformation for creating customer value continues to be a priority for many companies, as it enables them to gain a competitive advantage. While value creation is shaped by external drivers such as market volatility, technology, product and se作者: 笨重 時間: 2025-3-25 10:43 作者: –FER 時間: 2025-3-25 14:27 作者: DEFER 時間: 2025-3-25 18:01
Nicole Verdile,Anna Szabó,Rolando Pasquariello,Tiziana A. L. Brevini,Sandra Van Vlierberghe,Fulvio G some advanced logistics and supply chain management topics, recently mentioned by academic and industrial personnel. This book has been organized in 12 chapters such that the reader can study each chapter not only independently as shown in Fig. 1; but also as part of a whole. If someone wants to st作者: Genetics 時間: 2025-3-25 21:42 作者: 埋伏 時間: 2025-3-26 01:51
Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproduco be changed is the geometry: cells (whether primary, induced pluripotent, transformed or immortal) simply have to be grown in conditions that promote cell–cell adhesion while allowing gas, nutrient, signal, and metabolite exchange. Downstream analysis can become more complicated because many assays作者: 柳樹；枯黃 時間: 2025-3-26 08:02 作者: Hemiparesis 時間: 2025-3-26 11:43
A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydropf assisted reproduction techniques such as in vitro fertilization or even cloning of domestic animals by nuclear transfer from somatic cells. Currently, procedures of isolated immature ovarian follicles in vitro culture are becoming the prominent technology aimed to preserve or restore fertility esp作者: fringe 時間: 2025-3-26 13:24
Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres,culture technologies and materials. Presently, this approach can be applied to model human organ development and function “in a dish” and can be used to predict drug response in a patient specific fashion..Here we describe a protocol that allows for the derivation of functional cardiac mini organoid作者: 碎石 時間: 2025-3-26 20:16
Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Celouse is key to develop useful 3D culture tissue models to investigate uterine physiology and pathology without the need of performing invasive procedures to recover tissue samples..Here we provide a detail methodology that gives consistently pure and viable populations of distinct primary bovine end作者: 分期付款 時間: 2025-3-26 23:54
Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissuesaffolds to promote tissue development is the hallmark of the tissue engineering. Among the different types of scaffolds (derived from either natural or synthetic polymers) used in the field, the use of decellularized tissues/organs is specifically attractive. The decellularization process involves t作者: 乏味 時間: 2025-3-27 03:54 作者: 臆斷 時間: 2025-3-27 08:43
Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of e represented by the development of a bioprosthetic ovary, obtained through the combination of tissue engineering and regenerative medicine..We here describe the two main steps required for bioengineering the ovary and for its ex vivo functional reassembling. The first step aims at producing a 3D bi作者: 在駕駛 時間: 2025-3-27 13:05
A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells,of the placenta and supports the fetus during intrauterine life. Our understanding of these events is limited, particularly in human, because of ethical and legal restrictions and availability of adequate in vitro models would be very advantageous. Here we describe a method that converts human fibro作者: 左右連貫 時間: 2025-3-27 16:45 作者: 努力趕上 時間: 2025-3-27 21:23
Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometr adherent cells in cell biology are tailored to this format of cell culturing. Limitations and disadvantages of monolayer cultures, however, have resulted in the ongoing development of advanced cell culturing techniques. One such technique is culturing cells as multicellular spheroids, that had been作者: Entrancing 時間: 2025-3-28 00:39 作者: ARBOR 時間: 2025-3-28 02:05
Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columnells into the extracellular space. The population of EVs being heterogeneous poses a challenge in their efficient separation. Several methods have been employed for EV isolation. However, there is no single conventional method that could recover a high amount of EVs while retaining their purity and 作者: LEVY 時間: 2025-3-28 07:17
Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanopa potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) is a commonly used technique to measure the size and concentration of nanoparticles, such as EVs. Here, we present two protocols for the analysis of size?profile concentration, and zeta potential (ZP) of well-characterized EVs作者: avenge 時間: 2025-3-28 11:10
Isolation, Characterization, and MicroRNA Analysis of Extracellular Vesicles from Bovine Oviduct ancell type and tissue, and are involved in physiological and pathological processes. In recent years, EVs have been identified in reproductive tissues, such as oviduct and uterus, and have been shown to be related to several events important for reproductive success. The understanding of their functi作者: 顯而易見 時間: 2025-3-28 18:37 作者: FRAX-tool 時間: 2025-3-28 21:06 作者: 影響 時間: 2025-3-29 01:05
Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Modele the opportunity of studying complex biological systems in vitro, thereby reducing the need for animal experiments. Artificial intestine models are at the core of Fish-AI, an EU FET-Open research project dedicated to the development of a 3D in vitro platform that is intended to enable the aquacultu作者: Baffle 時間: 2025-3-29 07:06 作者: Armory 時間: 2025-3-29 08:15 作者: 忙碌 時間: 2025-3-29 14:20 作者: Oligarchy 時間: 2025-3-29 16:15
Book 2021ratory that mimic cell guidance conditions as they occur .in vivo.. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other se作者: glomeruli 時間: 2025-3-29 21:38 作者: 螢火蟲 時間: 2025-3-30 00:03
A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells,ne morphogenetic protein 4 (BMP4) with inhibitors of the Activin/Nodal/ERK signaling pathways, to drive erased fibroblasts into the trophoblastic differentiation. This innovative method uses very easily accessible cells to derive trophoblast-like cells and it can be useful to study embryo implantation disorders related to aging.作者: 該得 時間: 2025-3-30 04:24
Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columnication as it recovers a sufficient amount of EVs while retaining their functionality. Here, we describe a method of purification of EVs from bovine follicular fluid samples using benchtop SEC columns. In conclusion, the EV purification method should be chosen based on the downstream applications, as every method poses its own limitations.作者: Observe 時間: 2025-3-30 10:26 作者: 遺傳學 時間: 2025-3-30 15:50 作者: –吃 時間: 2025-3-30 20:37 作者: Pert敏捷 時間: 2025-3-30 22:44
Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissuesropriate biological signals and mechanical properties of the original tissue. Here, we describe different methods to create viable scaffolds from decellularized heart and liver as useful tools to study and exploit ECM biological key factors for the generation of engineered tissues with enhanced regenerative properties.作者: faultfinder 時間: 2025-3-31 01:11
3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids,eated from HepG2/C3A human hepatocytes or coculture of HepG2/C3A and endothelial cell line HMEC-1. It includes protocol for spheroids disassembling, labeling of cells with fluorescein diacetate and propidium iodide and instructions for flow cytometry gating optimized for analysis of heterogeneous cell populations form spheroids.作者: 匍匐 時間: 2025-3-31 08:19
Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometrcomet assay, transcriptomics, proteomics and lipidomic. This fast and gentle disassembly of intact spheroids into rim and core fractions, and further into viable single-cell suspension provides an opportunity to bridge the gap from 3D cell culture to current state-of-the-art analysis methods.作者: gnarled 時間: 2025-3-31 12:50 作者: negligence 時間: 2025-3-31 17:21 作者: Mawkish 時間: 2025-3-31 18:13 作者: 畢業(yè)典禮 時間: 2025-3-31 23:40 作者: investigate 時間: 2025-4-1 03:14
Matteo Ghiringhelli,Yousef Abboud,Snizhanna V. Chorna,Irit Huber,Gil Arbel,Amira Gepstein,Georgia Pe, Kontrolle und Optimierung des gesamten Supply Network vom Endkunden bis hin zu den Sublieferanten der Vormaterialien. Nur in Verbindung mit effizienten und durchg?ngigen Logistiksystemen k?nnen die heutigen Auftr?ge auch termintreu und zuverl?ssig bew?ltigt werden. Daher ist die Einbindung leistun作者: Insensate 時間: 2025-4-1 08:33
Georgia Pennarossa,Alireza Fazeli,Sergio Ledda,Fulvio Gandolfi,Tiziana A. L. Brevini, Kontrolle und Optimierung des gesamten Supply Network vom Endkunden bis hin zu den Sublieferanten der Vormaterialien. Nur in Verbindung mit effizienten und durchg?ngigen Logistiksystemen k?nnen die heutigen Auftr?ge auch termintreu und zuverl?ssig bew?ltigt werden. Daher ist die Einbindung leistun作者: prick-test 時間: 2025-4-1 14:01
Georgia Pennarossa,Matteo Ghiringhelli,Fulvio Gandolfi,Tiziana A. L. Breviniic Kanbans, inventory models, scheduling, purchasing and contracts. To facilitate easy reading, the chapters that deal with supply chain related issues are first, followed by studies on inventory, scheduling, purchasing and logistics. .This book can be used as a useful resource of supply chain manag作者: Latency 時間: 2025-4-1 15:39
