標(biāo)題: Titlebook: New Nucleic Acid Techniques; John M. Walker Book 1988 Springer Science+Business Media New York 1988 [打印本頁(yè)] 作者: 熱情美女 時(shí)間: 2025-3-21 16:16
書(shū)目名稱(chēng)New Nucleic Acid Techniques影響因子(影響力)
作者: 權(quán)宜之計(jì) 時(shí)間: 2025-3-21 21:25 作者: 亂砍 時(shí)間: 2025-3-22 03:31
New Nucleic Acid Techniques978-1-59259-491-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Obliterate 時(shí)間: 2025-3-22 08:25 作者: escalate 時(shí)間: 2025-3-22 11:26
Use of Elutips to Purify DNA,l techniques for manipulation of DNA. The Elutip-d columns commercially available are manufactured by Schleicher and Schuell. Each column consists of a pipet tip, such as may be used on a 0–200 μL automatic pipet, that is filled with an insoluble matrix.作者: 獨(dú)特性 時(shí)間: 2025-3-22 14:24
Jeffrey W. Pollard,Caroline R. Perry,Christopher F. Thurstongrable submartingales are considered, and only elementary facts ofthe square integrable functions are used in the proof. In stochastic differential equations, the Euler–Maruyama approximation is used mainly to prove the uniqueness of martingale problems and the smoothness of solutions of stochastic 作者: 搬運(yùn)工 時(shí)間: 2025-3-22 17:38
Adrian Slatergrable submartingales are considered, and only elementary facts ofthe square integrable functions are used in the proof. In stochastic differential equations, the Euler–Maruyama approximation is used mainly to prove the uniqueness of martingale problems and the smoothness of solutions of stochastic 作者: 發(fā)生 時(shí)間: 2025-3-23 00:47
Christopher F. Thurston,Caroline R. Perry,Jeffrey W. Pollard作者: nocturnal 時(shí)間: 2025-3-23 03:45 作者: epidermis 時(shí)間: 2025-3-23 06:03
Direct Dideoxy DNA Sequencing,eoxynucleoside triphosphates (ddNTPs), specific termination occurs at each of the four different nucleotides, and if this is carried out separately with each of the ddNTPs, then the four sets of reaction products can be analyzed on DNA sequencing gels.作者: Bucket 時(shí)間: 2025-3-23 12:22
Oligonucleotide Synthesis Using the Manual Phosphotriester Method,is because of the availability of highly reactive mononucleotides and specially developed coupling catalysts. In optimized systems, this allows for synthesis of oligomers greater than 50 bases in length.作者: condone 時(shí)間: 2025-3-23 15:53
Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde,uction allowed for major advances, most particularly in the molecular biology of eukaryotic organisms. The method had, nevertheless, two significant disadvantages in that the gels (composed of acrylamide at very low concentrations) were mechanically fragile, and the migration of RNA molecules did no作者: Militia 時(shí)間: 2025-3-23 19:51
Northern Blotting,A is first separated by electrophoresis under denaturing conditions (..), followed by blotting onto a suitable filter. Specific sequences are then detected by hybridization. The RNA species are both immobilized on the filter and denatured so that when the filter is immersed in a solution containing 作者: 叢林 時(shí)間: 2025-3-23 23:14 作者: sulcus 時(shí)間: 2025-3-24 06:10
Hybrid-Arrested Translation,, a specific messenger RNA in a cell-free system. In this respect the approach is similar to “hybrid selection,” also known as “hybrid release” (..), in which messenger RNAs homologous to cDNA clones are specifically trapped as RNA/DNA hybrids on nitrocelloulose filters. By washing the filters at sp作者: 無(wú)畏 時(shí)間: 2025-3-24 07:18
In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyties). Both these systems have a number of advantages, including the ease and cheapness of preparation, the relative absence of RNAse, the high level of reinitiation of ribosomes onto mRNA, the high fidelity of translation, and the maintenance of activity upon long-term storage. Both of them have als作者: 鑲嵌細(xì)工 時(shí)間: 2025-3-24 13:40
Use of Elutips to Purify DNA,l techniques for manipulation of DNA. The Elutip-d columns commercially available are manufactured by Schleicher and Schuell. Each column consists of a pipet tip, such as may be used on a 0–200 μL automatic pipet, that is filled with an insoluble matrix.作者: defendant 時(shí)間: 2025-3-24 18:47
Plasmid Preparation on Sephacryl S1000, for plasmid purification, all of which have common features; namely, (.) growth of plasmid-containing bacteria, (.) lysis of bacteria, (.) low-speed centrifugation to remove the majority of bacterial DNA and protein, (4) an ultracentrifugation step in cesium chloride and ethidium bromide to separat作者: 參考書(shū)目 時(shí)間: 2025-3-24 21:42
Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels,ly adequate. As the size of the fragments of interest decreases to below 1000 base pairs (bp), however, the resolution deteriorates significantly, and an alternative is required. Polyacrylamide gels from 5% acrylamide upward provide a convenient system for analysis of fragments down to 10 bp. This t作者: Inclement 時(shí)間: 2025-3-24 23:42
Use of 35S Nucleotides for DNA Sequencing,d are known as the Maxam-Gilbert method of chemical cleavage (.) and the dideoxy chain termination method developed originally by Sanger (.). One of the most critical steps in either of these two methods is the radiolabeling of the DNA such that the sequencing gel “l(fā)adder” of reaction products may b作者: 母豬 時(shí)間: 2025-3-25 07:14 作者: 考得 時(shí)間: 2025-3-25 08:44
Computer Applications to Molecular Biology,ed, and the most recent issue of the . (release 10, 1987) contains almost 10 million base pairs in almost 9000 entries compared with the first release from 1982 containing half a million base pairs in 500 entries. The requirement for computers is obvious not only from the total amount of DNA sequenc作者: Chronological 時(shí)間: 2025-3-25 13:04 作者: Perigee 時(shí)間: 2025-3-25 18:00 作者: 加強(qiáng)防衛(wèi) 時(shí)間: 2025-3-25 23:01
Manual Oligonucleotide Synthesis Using the Phosphoramidite Method,exocyclic amino groups on cytosine and the purines are protected as the alkali-labile benzoyl or .-butyryl amides. The 3′-OH group of the first nucleoside is attached to the solid support by a spacer chain, and the synthesis procedes by coupling the 5′-OH group of the growing chain to the 3′-phospho作者: 聯(lián)邦 時(shí)間: 2025-3-26 02:45
Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis,uction of novel linkers, site-directed mutagenesis, and many other techniques. The material obtained from a particular synthesis contains traces of premature termination products and other unwanted byproducts of the organic reactions. For most uses in genetic engineering, the material must be purifi作者: libertine 時(shí)間: 2025-3-26 06:44 作者: 小卒 時(shí)間: 2025-3-26 11:53
,Construction of Mammalian Genomic Libraries Using λ Replacement Vectors,ideal state can be approached by cutting the DNA randomly and cloning large pieces of this DNA into a suitable vector (.). The DNA can be cleaved either by mechanical shearing, in which case the DNA is fragmented in a truely random fashion, but with the introduction of problems associated with blunt作者: 責(zé)難 時(shí)間: 2025-3-26 14:57
Cosmid Library Construction,ely from bacteriophage λ, where nonessential intergenic regions were identified. It had been noted that only λ DNA 37–52 kb in length (or 78–102% of the wild-type length) were efficiently packaged and passaged in vivo. Thus vectors from which the intergenic region had been removed could be positivel作者: 倫理學(xué) 時(shí)間: 2025-3-26 17:15 作者: 銀版照相 時(shí)間: 2025-3-26 21:47
Jeffrey W. Pollard,Caroline R. Perry,Christopher F. Thurstonlts in probability theory are reviewed. Then, it?follows a discussion of discrete-time martingales, continuous time square integrable martingales (particularly, continuous martingales of continuous paths), stochastic integrations with respect to continuous local martingales, and stochastic different作者: 終端 時(shí)間: 2025-3-27 02:00
Adrian Slaterlts in probability theory are reviewed. Then, it?follows a discussion of discrete-time martingales, continuous time square integrable martingales (particularly, continuous martingales of continuous paths), stochastic integrations with respect to continuous local martingales, and stochastic different作者: START 時(shí)間: 2025-3-27 09:10 作者: 不整齊 時(shí)間: 2025-3-27 11:42 作者: GEON 時(shí)間: 2025-3-27 17:02 作者: Occipital-Lobe 時(shí)間: 2025-3-27 20:45 作者: 格言 時(shí)間: 2025-3-28 00:11 作者: opprobrious 時(shí)間: 2025-3-28 02:23 作者: aristocracy 時(shí)間: 2025-3-28 08:47
Plasmid Preparation on Sephacryl S1000,5) of this procedure are expensive, time-consuming, and require expensive machinery [ultracentrifuge, rotor(s)]. In addition, incomplete extraction of ethidium bromide can result in plasmid DNA that is not manipulable by many enzymes commonly used in DNA research.作者: 猛烈責(zé)罵 時(shí)間: 2025-3-28 13:24
Manual Oligonucleotide Synthesis Using the Phosphoramidite Method,ions. When the synthesis is completed, the chain must be cleaved from the support and the base, phosphorus, and terminal 5′-OH-protecting groups removed. The desired oligonucleotide is then separated from the mixture of shorter chains and modified chains (.).作者: 希望 時(shí)間: 2025-3-28 16:42 作者: 瘋狂 時(shí)間: 2025-3-28 20:48 作者: lanugo 時(shí)間: 2025-3-28 22:59 作者: A簡(jiǎn)潔的 時(shí)間: 2025-3-29 03:47
Computer Applications to Molecular Biology,ed, but also when taking into account the number of bases needed in order to organize a gene. The size of a standard gene with no intervening sequences is 500–1000 base pairs, whereas interrupted genes may be 10 times longer.作者: 設(shè)想 時(shí)間: 2025-3-29 10:29 作者: 獸群 時(shí)間: 2025-3-29 13:39
Hybrid-Arrested Translation,s is that, whereas in hybrid selection translation reveals one or a few polypeptides, hybrid-arrested translation identifies a gene product by its disappearance from the pattern of translation products.作者: 極大的痛苦 時(shí)間: 2025-3-29 17:44 作者: 通知 時(shí)間: 2025-3-29 21:10
6樓作者: Serenity 時(shí)間: 2025-3-30 00:22
6樓作者: 付出 時(shí)間: 2025-3-30 06:51
7樓作者: SEMI 時(shí)間: 2025-3-30 08:20
7樓作者: groggy 時(shí)間: 2025-3-30 12:34
7樓作者: BROW 時(shí)間: 2025-3-30 19:58
7樓作者: geometrician 時(shí)間: 2025-3-30 23:44
8樓作者: 賞錢(qián) 時(shí)間: 2025-3-31 02:27
8樓作者: neolith 時(shí)間: 2025-3-31 06:55
8樓作者: occult 時(shí)間: 2025-3-31 10:03
8樓作者: 的染料 時(shí)間: 2025-3-31 16:47
9樓作者: 艦旗 時(shí)間: 2025-3-31 19:29
9樓作者: 表臉 時(shí)間: 2025-3-31 22:43
9樓作者: 大酒杯 時(shí)間: 2025-4-1 05:32
9樓作者: Pituitary-Gland 時(shí)間: 2025-4-1 07:44
10樓作者: 尖叫 時(shí)間: 2025-4-1 13:54
10樓作者: ECG769 時(shí)間: 2025-4-1 16:41
10樓作者: Graduated 時(shí)間: 2025-4-1 21:54
10樓
