標(biāo)題: Titlebook: Neurotransmitter Methods; Richard C. Rayne Book 1997 Humana Press 1997 [打印本頁(yè)] 作者: 萬靈藥 時(shí)間: 2025-3-21 17:56
書目名稱Neurotransmitter Methods影響因子(影響力)
作者: tenuous 時(shí)間: 2025-3-21 22:38
Alexandra I. M. Breukel,Elly Besselsen,Wim E. J. M. Ghijsen作者: 高貴領(lǐng)導(dǎo) 時(shí)間: 2025-3-22 01:55
Sophie Sarre,Katrien Thorré,Ilse Smolders,Yvette Michotte作者: cinder 時(shí)間: 2025-3-22 04:52 作者: 桶去微染 時(shí)間: 2025-3-22 11:40
David G. Watson,Richard A. Baines,John M. Midgley,Jonathan P. Bacon作者: 不能仁慈 時(shí)間: 2025-3-22 13:08
Preparation of Brain Slices,udied can be located, identified, and accessed easily. Use of the brain slice has greatly increased our knowledge of the mammalian central nervous system (CNS). This technique is continuously improving and will remain valuable for a long time.作者: cluster 時(shí)間: 2025-3-22 17:51
Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method, access to the slice. Most studies now employ variations of the membrane filter method (.). Brain slices are placed on a porous membrane filter that provides a suitable substratum and allows a thin film of media to cover the surface of the slice by capillary action (.). The membrane inserts are plac作者: Shuttle 時(shí)間: 2025-3-22 23:44
Synaptosomes,ion, synaptosomes maintain a normal membrane potential (which is regulated by a Na./K.-ATPase) and express functional uptake carriers and ion-channels in their plasma-membranes. On application of diverse depolarizing stimuli (e.g., potassium, veratridine, and 4-aminopyridine), Ca. enters synaptosome作者: Peak-Bone-Mass 時(shí)間: 2025-3-23 04:26
Synaptoneurosomes,perties and a maintained electrochemical gradient (.,.). Since its first description, this preparation has been used for the investigation of numerous phenomena, including neurotransmitter release (.), inositol phospholipid turnover (.,.), CAMP accumulation (.), as well as in functional studies of v作者: Lumbar-Stenosis 時(shí)間: 2025-3-23 07:32
Detection of Neuropeptides by Immunocytochemistry, relied on ICC, for example: Different neuropeptides have very specific distributions in small populations of neurons (.–.), neuropeptides are commonly colocalized with low-mol-wt neurotransmitters or other neuropeptides (.), the chemical diversity of neurons is far greater than previously suspected作者: Gentry 時(shí)間: 2025-3-23 10:58
cGMP-Immunocytochemistry,th serotonim (.–.) and also with small peptides (.). Because the covalent modification of the hapten may be part of the antigenic determinant, it is a prerequisite that antisera are raised against conjugates prepared using a fixative that is also suitable for tissue fixation (.–.).作者: 排出 時(shí)間: 2025-3-23 15:48
Microwave Antigen Retrieval in Formaldehyde-Fixed Human Brain Tissue,ormalin fixation, however, the loss of antigenicity is neither complete nor irreversible. Indeed, formaldehydefixed tissue has been successfully used for the detection of many different tissue antigens, such as neurotransmitters (.), neuropeptides (.), cell surface markers (.), and enzymes (.). The 作者: 描繪 時(shí)間: 2025-3-23 19:37
Book 1997lthough you may find absent any mention of your favorite neurotransmitter, many of the protocols are sufficiently general to be adapted to alternative uses. So, how does this book contribute usefiilly to the horde of methods volumes stampeding across our bookshelves? 1 hope that the strength oiNeuro作者: Mere僅僅 時(shí)間: 2025-3-24 00:29
Neurotransmitter Methods978-1-59259-558-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 出血 時(shí)間: 2025-3-24 06:00
Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/664573.jpg作者: 感激小女 時(shí)間: 2025-3-24 07:56 作者: 雪白 時(shí)間: 2025-3-24 11:55
Neuropeptide Expression Patterns as Determined by Matrix-Assisted Laser Desorption Ionization Mass tage of these methods, however, is that they give limited clues to the structural identities of processed and biologically active peptides contained in the cells. Moreover, these techniques do not reveal previously undescribed cotransmitters.作者: AVANT 時(shí)間: 2025-3-24 16:51
The Preparation and Use of Brain Slices and Dissociated Neurons for Patch-Clamp Studies of Neurotraevelopment of the patch-clamp technique has opened up the possibility of direct access to the intracellular environment via internal patch pipet solutions. In combination, these approaches have enabled detailed investigations of neuronal membrane properties, the cellular actions of neurotransmitters, and synaptic mechanisms.作者: 周興旺 時(shí)間: 2025-3-24 22:08 作者: 極大痛苦 時(shí)間: 2025-3-24 23:45
Microbore Liquid Chromatography Analysis of Monoamine Transmitters,ional LC: The sample injected on to a microbore column is diluted by about 20-fold less than when injected on to a “normal” bore (e.g., 4.6 mm id) column. In this way, the compounds of interest become easier to detect. Microbore LC offers several other advantages:作者: Nebulizer 時(shí)間: 2025-3-25 06:42 作者: misshapen 時(shí)間: 2025-3-25 08:02 作者: judicial 時(shí)間: 2025-3-25 15:06 作者: Collar 時(shí)間: 2025-3-25 16:28 作者: 廢除 時(shí)間: 2025-3-25 22:45 作者: Rankle 時(shí)間: 2025-3-26 00:49 作者: stress-response 時(shí)間: 2025-3-26 07:49
GC/MS Determination of Biogenic Amines in insect Neurons,ntage in the detection of such biomolecules over similarly sensitive techniques, such as HPLC-ECD, because GC-MS relies not only on chromatographic retention time but also on molecular mass to ascertain identity.作者: 檔案 時(shí)間: 2025-3-26 11:57 作者: bacteria 時(shí)間: 2025-3-26 15:50 作者: stress-test 時(shí)間: 2025-3-26 18:30 作者: CYN 時(shí)間: 2025-3-27 00:51
Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method,ents are performed using brain slices soon after their preparation. Thin brain slices have limited viability: Unless special culture conditions are employed, slices die after 1 d in vitro (DIV) (..). Longer viability is often desired to permit study of more extended processes, such as long-term modu作者: Encapsulate 時(shí)間: 2025-3-27 01:54 作者: 命令變成大炮 時(shí)間: 2025-3-27 05:28
Synaptosomes,sealed particles that contain small, clear vesicles and sometimes larger densecore vesicles, indicating their presynaptic origin. Occasionally, fractions of electron-dense, postsynaptic membranes remain attached to the synaptosomes, facing the active zone. Evidently, synaptosomes contain all the com作者: ELUDE 時(shí)間: 2025-3-27 09:53
Synaptoneurosomes,e not suited for the investigation of synaptic events mediated by postsynaptic mechanisms. In the past, isolated membrane fractions enriched in postsynaptic densities have been used for this purpose (.). However, this fraction of postsynaptic densities is not appropriate to study receptor-mediated s作者: 逃避系列單詞 時(shí)間: 2025-3-27 14:52
Preparation of Synaptosomal Plasma Membranes by Subcellular Fractionation,res some purification in order to study neuronal and glial functioning in isolation at the molecular level. The introduction of the ultracentrifuge by Svedberg in 1925 offered neurochemists for the first time the ability to fractionate homogenized brain tissue into its subcellular components. Furthe作者: 王得到 時(shí)間: 2025-3-27 21:24
Detection of Neuropeptides by Immunocytochemistry,t neuropeptides have important roles as neurohormones, neuromodulators, cytokines, morphogenetic factors, and possibly in some cases, as true neurotransmitters. Each neuropeptide may even be multifunctional and exist in several isoforms in a given animal species. In the search for functions of neuro作者: URN 時(shí)間: 2025-3-28 01:01 作者: Anecdote 時(shí)間: 2025-3-28 02:16
cGMP-Immunocytochemistry,protein antigens are relatively insensitive to the type of fixative used. Apparently, the epitopes are not altered in a decisive way by the fixative, immobilization is usually effective, and more than one eptiope may be recognized by polyclonal antisera. In studying by ICC the location of small, hyd作者: CHARM 時(shí)間: 2025-3-28 07:35
Microwave Antigen Retrieval in Formaldehyde-Fixed Human Brain Tissue,tuations, at varying concentrations and with virtually all tissues, it has remained one of the most widely used fixatives. Formaldehyde is a reactive electrophilic species (.,.) that reacts readily with primary amines, amide groups, aromatic rings, and alcoholic hydroxyl groups, crosslinking various作者: sperse 時(shí)間: 2025-3-28 13:53
Localization of Nitric Oxide Synthase Using NADPH Diaphorase Histochemistry,990, the enzyme responsible for synthesis of NO in the brain, NO synthase (NOS), was purified and antibodies to it were used to establish the distribution of NO-producing neurons in the rat brain (.). NOS was also found to be responsible for an enzyme activity in the brain known as NADPH diaphorase 作者: 沉積物 時(shí)間: 2025-3-28 18:25 作者: conquer 時(shí)間: 2025-3-28 22:48 作者: 欲望小妹 時(shí)間: 2025-3-29 01:31 作者: Adornment 時(shí)間: 2025-3-29 03:57
Microbore Liquid Chromatography Analysis of Amino Acid Transmitters,of amino acid transmitters in the microdialysates requires very sensitive methods of analysis owing to the subpicogram amounts of amino acids in small volumes of dialysates (in our experimental setup, the total volume is usually 40 μL). Often, conventional liquid chromatography (LC) systems are unab作者: Frequency-Range 時(shí)間: 2025-3-29 09:56 作者: Hemiplegia 時(shí)間: 2025-3-29 15:17
Neuropeptide Expression Patterns as Determined by Matrix-Assisted Laser Desorption Ionization Mass Currently, immunocytochemistry and . hybridization are the methods of choice to map neuropeptide expression patterns in the nervous system. A disadvantage of these methods, however, is that they give limited clues to the structural identities of processed and biologically active peptides contained i作者: 繁忙 時(shí)間: 2025-3-29 17:39
GC/MS Determination of Biogenic Amines in insect Neurons,ervous systems of almost all multicellular animals. In the insect central nervous system (CNS), the presence of some of these amines (dopamine and octopamine) has been inferred by a variety of sensitive, although somewhat nonspecific techniques, including immunohistochemical and histofluorimetric st作者: 流浪 時(shí)間: 2025-3-29 19:46 作者: 上漲 時(shí)間: 2025-3-30 01:30
6樓作者: tympanometry 時(shí)間: 2025-3-30 05:28
6樓作者: 信徒 時(shí)間: 2025-3-30 09:12
6樓作者: 無彈性 時(shí)間: 2025-3-30 13:18
7樓作者: GLARE 時(shí)間: 2025-3-30 18:55
7樓作者: ANT 時(shí)間: 2025-3-30 21:01
7樓作者: circumvent 時(shí)間: 2025-3-31 01:39
7樓作者: 史前 時(shí)間: 2025-3-31 05:57
8樓作者: modifier 時(shí)間: 2025-3-31 09:25
8樓作者: landfill 時(shí)間: 2025-3-31 15:40
8樓作者: 令人不快 時(shí)間: 2025-3-31 19:26
8樓作者: 不如屎殼郎 時(shí)間: 2025-4-1 00:37
9樓作者: 槍支 時(shí)間: 2025-4-1 02:15
9樓作者: Hemodialysis 時(shí)間: 2025-4-1 09:44
9樓作者: 不能仁慈 時(shí)間: 2025-4-1 11:11
9樓作者: 減去 時(shí)間: 2025-4-1 16:34
10樓作者: Phenothiazines 時(shí)間: 2025-4-1 20:23
10樓作者: 有害 時(shí)間: 2025-4-2 02:00
10樓作者: MUTED 時(shí)間: 2025-4-2 06:47
10樓
