標題: Titlebook: Natural Killer Cells; Methods and Protocol Srinivas S. Somanchi Book 2016 Springer Science+Business Media New York 2016 cytotoxicity.genoty [打印本頁] 作者: Boldfaced 時間: 2025-3-21 16:29
書目名稱Natural Killer Cells影響因子(影響力)
書目名稱Natural Killer Cells影響因子(影響力)學科排名
書目名稱Natural Killer Cells網(wǎng)絡公開度
書目名稱Natural Killer Cells網(wǎng)絡公開度學科排名
書目名稱Natural Killer Cells被引頻次
書目名稱Natural Killer Cells被引頻次學科排名
書目名稱Natural Killer Cells年度引用
書目名稱Natural Killer Cells年度引用學科排名
書目名稱Natural Killer Cells讀者反饋
書目名稱Natural Killer Cells讀者反饋學科排名
作者: 總 時間: 2025-3-22 00:18
Aimee M. Beaulieu,Joseph C. Sun Ph.D.used in cryptographic primitives and certificates used by the web servers have been reported. The approach was to probe all web servers using a tool - TestSSLServer. There were sets of two experiments carried out. One in which top 500 most popular websites in India were probed and other in which 50 作者: FER 時間: 2025-3-22 03:04
Alexander W. Kay,Dara M. Strauss-Albee,Catherine A. Blish proposed scheme is based on encryption of data, keyword generation and search based on bilinear pairing. The system provides a proxy server which manipulates user queries making the system faster and more secure. The search is performed on keyword ordered list which makes the search faster as compa作者: 乳白光 時間: 2025-3-22 06:48 作者: 情感 時間: 2025-3-22 11:16 作者: 大廳 時間: 2025-3-22 16:54
Sourindra N. Maiti Ph.D.the data that comes from weather stations owned by the residents of the monitored area, that was designed using Internet of Things technology. This approach creates new, unprecedented threats to information security and system continuity. In addition to threats resulting from the use of the Internet作者: 裙帶關系 時間: 2025-3-22 19:26 作者: Entropion 時間: 2025-3-22 21:32 作者: 壯麗的去 時間: 2025-3-23 03:41 作者: impaction 時間: 2025-3-23 05:54
The Planar Lipid Bilayer System Serves as a Reductionist Approach for Studying NK Cell Immunologicaudying the dynamics of synapse formation, and (3) they allow investigators to determine the exact composition of the bilayer, thus in turn allowing them to answer very specific questions about the IS. It is our hope that this chapter will furnish readers with an awareness of the applications of the 作者: euphoria 時間: 2025-3-23 11:59
Expansion of NK Cells Using Genetically Engineered K562 Feeder Cells, and 4-1BBL, coexpressed by K562 cells, acted synergistically to augment K562-specific NK stimulatory capacity, resulting in vigorous expansion of peripheral blood CD56. CD3. NK cells without concomitant growth of T lymphocytes. Here, we describe an in vitro expansion method of human NK cells among 作者: 內向者 時間: 2025-3-23 16:20 作者: 專心 時間: 2025-3-23 19:50 作者: 疲勞 時間: 2025-3-24 01:17
Book 2016r chemokine receptors using?retroviral vectors,electroporation of mRNA, and the natural phenomenon of trogocytosis.?Written in the highly successful?.Methods in Molecular Biology?.series format, these chapters include introductions to their respective topics, lists of the necessary materials and rea作者: 發(fā)微光 時間: 2025-3-24 02:41 作者: 獨裁政府 時間: 2025-3-24 09:14 作者: meretricious 時間: 2025-3-24 12:00 作者: 墊子 時間: 2025-3-24 16:15
Minh-Trang Thi Phan,Seung-Hwan Lee,Sang-Ki Kim D.V.M., Ph.D.,Duck Cho M.D., Ph.D.作者: BALK 時間: 2025-3-24 22:26
Natalia Lapteva Ph.D.,Robin Parihar,Lisa A. Rollins,Adrian P. Gee,Cliona M. Rooney作者: 解決 時間: 2025-3-25 01:20 作者: hematuria 時間: 2025-3-25 06:59
978-1-4939-8116-8Springer Science+Business Media New York 2016作者: MAG 時間: 2025-3-25 09:18 作者: 裝勇敢地做 時間: 2025-3-25 15:08 作者: compassion 時間: 2025-3-25 16:34
https://doi.org/10.1007/978-1-4939-3684-7cytotoxicity; genotyping single nucleotide polymorphisms; chemeric antigen receptors; retroviral vector作者: 摻和 時間: 2025-3-25 21:20 作者: Pcos971 時間: 2025-3-26 01:57
Tracking Effector and Memory NK Cells During MCMV Infection,ansion of Ly49H. NK cells peaks at approximately 1 week post-infection, and is then followed by a distinct contraction phase that ultimately leaves a small but long-lived pool of MCMV-experienced Ly49H. NK cells that are capable of mediating enhanced memory-like responses during subsequent encounter作者: EXTOL 時間: 2025-3-26 06:21
Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cellconjugated to monoclonal antibodies to evaluate up to 42 parameters simultaneously on individual cells with minimal overlap between channels. The platform can be customized for analysis of both phenotypic and functional markers. Here, we will describe methods to stain, collect, and analyze intracell作者: 粘土 時間: 2025-3-26 11:33
Assessment of NK Cell Metabolism, others have begun to investigate the metabolic requirements for NK cell differentiation and activation. Here, we describe approaches for the in vitro assessment of NK cell metabolism. We present methods for using inhibitors to alter cellular metabolism, measurement of intracellular ATP in NK cells,作者: Contort 時間: 2025-3-26 13:06 作者: 強制令 時間: 2025-3-26 17:43
Measurement of Average Telomere Length in Ex Vivo Expanded Natural Killer Cells by Fluorescence In through cytokine secretion and target cell lysis. NK cells function without any need for prior antigen exposure. Thus, more recently NK cells are considered a promising source of lymphocytes for adoptive tumor therapy. However, because NK cells represent only a small lymphocyte fraction, expand poor作者: 煩憂 時間: 2025-3-26 23:14
In Vitro Assessment of Human Natural Killer Cell Migration and Invasion,r invasion assays represent a reductionist system that allows for easy manipulation using various extracellular matrix (ECM) components addressing migratory functions or invasion into/through a three-dimensional matrix where migration and invasion inhibitors as well as stimulators can be added. Pres作者: Mitigate 時間: 2025-3-27 04:59
Microfluidic-Based Live-Cell Analysis of NK Cell Migration In Vitro,ments. NK cells express specific chemokine and non-chemokine receptors to aid preferential migrations or localizations in tissues. Good understanding of how NK-cell migratory properties are regulated in physiological and pathological microenvironments will provide further insights into the developme作者: aggressor 時間: 2025-3-27 05:49
Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotmetry, providing static snapshots. These approaches have certain drawbacks, not enabling dynamic observations of single cells over extended periods of time. One of the primary limitations of single cell imaging has been throughput; it has been challenging to collect data for many cells due to their 作者: 兩種語言 時間: 2025-3-27 11:18
Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry Method, cells is the direct cytolysis of their targets. Hence analyzing the cytotoxic potential of these lymphocytes is fundamental to understanding their biology and their clinical impact. We have previously shown that release-based cytotoxicity assays, such as calcein release assay, could potentially und作者: Communal 時間: 2025-3-27 15:37
Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry, cytokine production. Upon cellular activation, intracellular mobilization of Ca. ions initially involves depletion of endoplasmic reticulum stores, leading to subsequent Ca. influx through specific plasma membrane Ca. release activated Ca. channels. Multiple probes and assays for detecting intracel作者: wreathe 時間: 2025-3-27 18:17
Using NK Cell Lipid Raft Fractionation to Understand the Role of Lipid Rafts in NK Cell Receptor Sid not be confused with lipid rafts, DRMs are a valuable starting point for the study of these membrane domains and the interactions of proteins with rafts..Here we describe the isolation of DRMs by ultracentrifugation on a sucrose gradient, a method we have used to study the role of lipid rafts in N作者: 不可侵犯 時間: 2025-3-28 01:27 作者: Ostrich 時間: 2025-3-28 02:24
The Planar Lipid Bilayer System Serves as a Reductionist Approach for Studying NK Cell Immunologica or a tumor cell). The formation of the IS is crucial for cell-mediated immunity, and as such, an understanding of both the composition of the IS and the factors that drive its formation are essential for understanding how and when NK cells eliminate susceptible target cells. The supported lipid bil作者: acolyte 時間: 2025-3-28 07:08 作者: 基因組 時間: 2025-3-28 13:00
Ex Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21,rder to evaluate the therapeutic efficacy of NK cell adoptive immunotherapy in preclinical models with a potential for clinical translation, there is a need for a reliable platform for ex vivo expansion of NK cells. Numerous methods are reported in literature using cytokines and feeder cells to acti作者: Lime石灰 時間: 2025-3-28 15:16
Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy,nical protocols. Further, the ability to genetically modify primary human NK cells following rapid expansion allows targeting and enhancement of their immune function. We have successfully adapted an expansion method for primary NK cells from peripheral blood mononuclear cells or from apheresis prod作者: 擔憂 時間: 2025-3-28 21:12 作者: 榨取 時間: 2025-3-29 02:37 作者: 竊喜 時間: 2025-3-29 03:10
mRNA Transfection to Improve NK Cell Homing to Tumors,roenvironment where tumors reside. Recent studies have shown that cytokine-activated and ex vivo-expanded NK cells lack or express at low levels homing receptors required to achieve tissue-specific tumor targeting by cells administered intravenously. In this chapter, we describe a method to enhance 作者: corpus-callosum 時間: 2025-3-29 10:41
ed for the development of fast and reliable methods for primality testing to determine whether a given integer is prime or composite. Many algorithms were proposed by to address the efficient method of testing the primality of the integer number. In this paper, we propose a pipelined reconfigurable 作者: MEEK 時間: 2025-3-29 12:25 作者: circumvent 時間: 2025-3-29 19:35
Alexander W. Kay,Dara M. Strauss-Albee,Catherine A. Blishased solutions. Cloud computing provides the best alternative, providing storage as a service and ensures efficient data operations. Data outsourcing reduces high cost and increases efficiency but it is vulnerable to leakage and manipulation hence rendering it unusable for most of the practical appl作者: Anthrp 時間: 2025-3-29 22:17
Molly P. Keppel,Megan A. Cooper M.D., Ph.D.their servers and web browsers. It is very important to analyze the security of this protocol because the compromise of the banking accounts, health care directories, information of national importance, even vital information about business competitors is unacceptable..SSL/TLS is not a simple encryp作者: Hiatus 時間: 2025-3-30 02:48 作者: Kidnap 時間: 2025-3-30 07:08 作者: 感染 時間: 2025-3-30 09:19 作者: instill 時間: 2025-3-30 15:29
High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse,nts and the role they play in the regulation and execution of NK cell directed secretion. Here we describe a protocol for the recapitulation of the NK cell lytic synapse on glass, the acquisition of microscopy images, and suggested approaches for the processing and analysis of microscopy data.作者: labile 時間: 2025-3-30 19:04
Tracking Effector and Memory NK Cells During MCMV Infection,s with MCMV. Here we describe an adoptive transfer model in which the expansion, contraction, and memory cell persistence of transferred Ly49H. NK cells are tracked in congenic C57BL/6 hosts following MCMV infection.作者: 宏偉 時間: 2025-3-30 21:22
Assessment of NK Cell Metabolism, assessment of real-time glycolysis and oxidative phosphorylation by an extracellular flux assay from Seahorse Biosciences, and some basic protocols for stimulation of NK cells via cytokines and receptors.作者: 偉大 時間: 2025-3-31 02:06 作者: ostrish 時間: 2025-3-31 06:26
Book 2016new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study作者: infarct 時間: 2025-3-31 09:45
Ex Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21,K562 mb.IL21). We had previously demonstrated that this platform enables the highest fold expansion of NK cells reported in the literature to date (>47,000-folds in 21 days), and produces highly activated and pure NK cells without signs of senescence, as determined by telomere shortening.作者: 矛盾 時間: 2025-3-31 16:41 作者: Abrade 時間: 2025-3-31 19:46 作者: 特別容易碎 時間: 2025-3-31 22:49
Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry,pecific NK cell subsets is possible. Here, a flow cytometric method for dynamic measurements of intracellular Ca. concentrations in human NK cells subsets is detailed and discussed. This assay can be further adapted for specific scientific and diagnostic questions, with implications for various immunopathological conditions.
