作者: URN 時(shí)間: 2025-3-21 22:26
Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction,f EGFP or the transduced cell surface marker. Alternatively, cells expressing the transduced cDNAs can be selected for by treatment with neomycin, puromycin, or hygromycin. Using this method, the sorted/selected cells uniformly express the gene of interest and the expression is stable for many weeks of culture.作者: 繁忙 時(shí)間: 2025-3-22 00:26
Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils,elopment, as they exist in vivo, through analysis of NK cell maturational intermediates found in situ. Herein we describe a detailed, yet simple, four-step protocol for the viable enrichment and purification of human NK cell developmental intermediates from LNs and tonsils.作者: etiquette 時(shí)間: 2025-3-22 04:34 作者: Anterior 時(shí)間: 2025-3-22 12:00 作者: Alienated 時(shí)間: 2025-3-22 14:40 作者: 預(yù)防注射 時(shí)間: 2025-3-22 18:08
Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry,equently cross-linked by addition of streptavidin. The increase in intracellular calcium is quantified by measuring a shift in the indo-1 emission spectrum that takes place when the dye becomes bound to calcium.作者: 睨視 時(shí)間: 2025-3-22 23:32 作者: 繁榮中國(guó) 時(shí)間: 2025-3-23 02:58 作者: 征稅 時(shí)間: 2025-3-23 08:07 作者: accomplishment 時(shí)間: 2025-3-23 11:45 作者: Modicum 時(shí)間: 2025-3-23 15:34
Studying NK Cell/Dendritic Cell Interactions,vide an experimental guide to the analysis of NK cell/DC interactions in vitro and in vivo. We have put special emphasis on the recently developed mouse models allowing the inducible and specific ablation of various subsets of DCs and other myeloid cells.作者: alabaster 時(shí)間: 2025-3-23 20:16 作者: 為寵愛(ài) 時(shí)間: 2025-3-24 00:04
Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus,troviral vectors. After a single transduction with retrovirus, shRNA-containing cells can be selected with drug treatment or sorted for enhanced green fluorescent protein (EGFP) expression. With this method, protein expression can be stably decreased to less than 10% of wild-type levels.作者: 打谷工具 時(shí)間: 2025-3-24 04:19
1064-3745 ay of molecular and cellular techniques for manipulating and.As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addit作者: Omnipotent 時(shí)間: 2025-3-24 08:29
Book 2010Latest editionplaying important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functi作者: ELUC 時(shí)間: 2025-3-24 14:03 作者: lavish 時(shí)間: 2025-3-24 16:42 作者: Consensus 時(shí)間: 2025-3-24 22:15 作者: 徹底明白 時(shí)間: 2025-3-25 01:03 作者: 保守 時(shí)間: 2025-3-25 05:41 作者: conquer 時(shí)間: 2025-3-25 07:53
Identification of NK Cell Receptor Ligands Using a Signaling Reporter System,ands on target and effector cell surfaces. The identification of ligands for orphan NK cell receptors is key to enhancing our understanding of NK cell biology. Here we describe a strategy (protocol) for the identification of ligands for orphan NK cell receptors using signaling reporter cells in combination with a virus rescue system.作者: 錯(cuò) 時(shí)間: 2025-3-25 15:20 作者: negotiable 時(shí)間: 2025-3-25 19:21 作者: LARK 時(shí)間: 2025-3-25 22:49 作者: faction 時(shí)間: 2025-3-26 03:22 作者: 可互換 時(shí)間: 2025-3-26 06:10
Michael E. March,Catharina C. Gross,Eric O. Longentication for wireless sensor networks. Several practical tree-based broadcast authentication techniques were developed to support multiple senders, distribute parameters for .TESLA instances, and revoke the broadcast authentication capabilities of compromised senders in wireless sensor networks. O作者: synovium 時(shí)間: 2025-3-26 08:31
Deborah N. Burshtyn,Chelsea Davidsonodes usually have limited resources such as storage, bandwidth, computation and energy. It is often undesirable to implement expensive algorithms (e. g. , frequent public key operations) on sensor nodes. Second, sensor nodes are usually deployed unattended and built without compromise prevention in 作者: CLAP 時(shí)間: 2025-3-26 12:46
Mathias Lucas,Cedric Vonarbourg,Peter Aichele,Andreas Diefenbachentication for wireless sensor networks. Several practical tree-based broadcast authentication techniques were developed to support multiple senders, distribute parameters for .TESLA instances, and revoke the broadcast authentication capabilities of compromised senders in wireless sensor networks. O作者: 阻塞 時(shí)間: 2025-3-26 16:47
Alexander W. MacFarlane IV,James F. Oesterling,Kerry S. Campbellles in road traffic and the prevention of attacks against sensor networks positions. We show how this feature can be implemented by an appropriate combination of distance bounding and multilateration..The slides of the talk are available at http://lcawww.epfl.ch/hubaux/作者: pulmonary-edema 時(shí)間: 2025-3-26 21:06
Takuya Miyagi,Seung-Hwan Lee,Christine A. Biron and flexible architecture for service centric sensor networks. Key areas of application of the architecture are gradually extendable service centric sensor networks where sensors and actuators jointly perform various user defined tasks, e.g. in the field of an office environment or health care.作者: 忘恩負(fù)義的人 時(shí)間: 2025-3-27 04:11
Lukasz K. Chlewicki,Vinay Kumar1990 they were shocked by Iraq’s occupation of Kuwait and unanimous in seeking the pull-back of Iraqi forces. In Northern Ireland the parties sought a political accommodation which would end violence and diminish hostility between the two communities.作者: Somber 時(shí)間: 2025-3-27 09:17
S. M. Shahjahan Miah,Kerry S. Campbell1990 they were shocked by Iraq’s occupation of Kuwait and unanimous in seeking the pull-back of Iraqi forces. In Northern Ireland the parties sought a political accommodation which would end violence and diminish hostility between the two communities.作者: 逗它小傻瓜 時(shí)間: 2025-3-27 11:23
Ram Savan,Tim Chan,Howard A. Young1990 they were shocked by Iraq’s occupation of Kuwait and unanimous in seeking the pull-back of Iraqi forces. In Northern Ireland the parties sought a political accommodation which would end violence and diminish hostility between the two communities.作者: 喚起 時(shí)間: 2025-3-27 14:07 作者: 半球 時(shí)間: 2025-3-27 19:56
Sam K.P. Kung1990 they were shocked by Iraq’s occupation of Kuwait and unanimous in seeking the pull-back of Iraqi forces. In Northern Ireland the parties sought a political accommodation which would end violence and diminish hostility between the two communities.作者: synovium 時(shí)間: 2025-3-28 01:46
Dikla Lankry,Roi Gazit,Ofer Mandelboim1990 they were shocked by Iraq’s occupation of Kuwait and unanimous in seeking the pull-back of Iraqi forces. In Northern Ireland the parties sought a political accommodation which would end violence and diminish hostility between the two communities.作者: deforestation 時(shí)間: 2025-3-28 02:14
Alon Zilka,Michal Mendelson,Benyamin Rosental,Oren Hershkovitz,Angel Porgador1990 they were shocked by Iraq’s occupation of Kuwait and unanimous in seeking the pull-back of Iraqi forces. In Northern Ireland the parties sought a political accommodation which would end violence and diminish hostility between the two communities.作者: Between 時(shí)間: 2025-3-28 07:42
Yoshie-Matsubayashi Iizuka,Nikunj V. Somia,Koho Iizuka1990 they were shocked by Iraq’s occupation of Kuwait and unanimous in seeking the pull-back of Iraqi forces. In Northern Ireland the parties sought a political accommodation which would end violence and diminish hostility between the two communities.作者: 心神不寧 時(shí)間: 2025-3-28 12:06 作者: 猛然一拉 時(shí)間: 2025-3-28 14:37
Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and induced during viral infections as a result of IFN exposure, and that this change acts to promote the activation of STAT1 but limit both the activation of STAT4 and IFNγ expression. The intracellular staining approaches used for the studies described here have utility in characterizing other mechan作者: generic 時(shí)間: 2025-3-28 19:21 作者: infantile 時(shí)間: 2025-3-29 02:14
Book 2010Latest editiond techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume 作者: 倔強(qiáng)不能 時(shí)間: 2025-3-29 06:28
ned for a professional audience composed of practitioners and researchers in industry. This book is also suitable as a secondary text book or reference for advanced-level students in computer science and electrical engineering...?.978-1-4419-4438-2978-0-387-72442-3Series ISSN 1568-2633 Series E-ISSN 2512-2193 作者: Ganglion 時(shí)間: 2025-3-29 09:29 作者: BILL 時(shí)間: 2025-3-29 12:26 作者: 能得到 時(shí)間: 2025-3-29 16:59
1064-3745 ludes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume 978-1-4939-5624-1978-1-60761-362-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: certain 時(shí)間: 2025-3-29 20:37 作者: SPER 時(shí)間: 2025-3-30 03:13 作者: 最高峰 時(shí)間: 2025-3-30 04:41 作者: 譏諷 時(shí)間: 2025-3-30 12:00 作者: Herd-Immunity 時(shí)間: 2025-3-30 13:38
Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NKcells, the establishment of a well-defined protocol to replicate in vitro the major steps corresponding to the process of NK cell differentiation and maturation has enabled us to dissect the molecular events governing certain aspects of NK cell development. This chapter describes a protocol that com作者: EXALT 時(shí)間: 2025-3-30 20:32
Use of Transfected , S2 Cells to Study NK Cell Activation,ory NK cell receptors. Mammalian target cells typically express a variety of ligands for NK cell receptors. Engagement of NK cell receptors by antibodies may not mimic activation by natural ligands. To define requirements for activation and dissect the contribution of receptors to NK cell function, 作者: 廢除 時(shí)間: 2025-3-30 23:32 作者: Intend 時(shí)間: 2025-3-31 04:46
Analysis of the NK Cell Immunological Synapse, NK cell and the cell with which it is interacting. This interface is also known as the immunological synapse and is characterized by an extraordinary number of dynamic molecular events that have the potential to result in NK cell function. Here we describe microscopy-based methods for evaluating an作者: crumble 時(shí)間: 2025-3-31 08:54
Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry,ometer fitted with a UV laser. One major advantage of this method is that the calcium response of the minority NK cell population and even smaller NK cell subpopulations can be measured simultaneously from a mixture of freshly prepared total splenocytes without resorting to prior cell sorting or exp作者: 淘氣 時(shí)間: 2025-3-31 10:27 作者: lesion 時(shí)間: 2025-3-31 17:20 作者: 全國(guó)性 時(shí)間: 2025-3-31 18:23 作者: 儲(chǔ)備 時(shí)間: 2025-3-31 22:24
Lentiviral Gene Transduction in Human and Mouse NK Cell Lines,ce of these cells to gene transfer. Considering over-expression and gene knockdown studies are crucial tools to study the biology of cells, technologies suitable for transfering genes into NK cells are invaluable. Among various technologies available for gene transfer, lentiviral-mediated transducti作者: ASTER 時(shí)間: 2025-4-1 01:53 作者: Esophagus 時(shí)間: 2025-4-1 07:07
Introduction of shRNAs into Primary NK Cells with Lentivirus,ions of primary NK cell ex vivo had limited our studies of protein function(s) in NK cell differentiation, acquisition of self-tolerance, and induction of anti-tumor responses. We used VSV-G-pseudotyped, EGFP-expressing lentiviral vectors to develop an efficient gene transfer system to modify gene e作者: exclusice 時(shí)間: 2025-4-1 13:13
Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands,surface receptors. Similarly, the killing of NK cells is controled by multiple activating and inhibitory surface receptors. In humans, the major NK triggering receptors, identified so far, include NKp80, 2B4 NKG2D, and CD16 and the natural cytotoxic receptors (collectively named NCRs) include NKp46,作者: 發(fā)源 時(shí)間: 2025-4-1 18:01 作者: 粗野 時(shí)間: 2025-4-1 22:19
Identification of NK Cell Receptor Ligands Using a Signaling Reporter System,ands on target and effector cell surfaces. The identification of ligands for orphan NK cell receptors is key to enhancing our understanding of NK cell biology. Here we describe a strategy (protocol) for the identification of ligands for orphan NK cell receptors using signaling reporter cells in comb
