標(biāo)題: Titlebook: Nanotechnology for Nucleic Acid Delivery; Methods and Protocol Manfred Ogris,Haider Sami Book 2019Latest edition Springer Science+Business [打印本頁] 作者: Disaster 時(shí)間: 2025-3-21 16:05
書目名稱Nanotechnology for Nucleic Acid Delivery影響因子(影響力)
書目名稱Nanotechnology for Nucleic Acid Delivery影響因子(影響力)學(xué)科排名
書目名稱Nanotechnology for Nucleic Acid Delivery網(wǎng)絡(luò)公開度
書目名稱Nanotechnology for Nucleic Acid Delivery網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Nanotechnology for Nucleic Acid Delivery被引頻次
書目名稱Nanotechnology for Nucleic Acid Delivery被引頻次學(xué)科排名
書目名稱Nanotechnology for Nucleic Acid Delivery年度引用
書目名稱Nanotechnology for Nucleic Acid Delivery年度引用學(xué)科排名
書目名稱Nanotechnology for Nucleic Acid Delivery讀者反饋
書目名稱Nanotechnology for Nucleic Acid Delivery讀者反饋學(xué)科排名
作者: FACET 時(shí)間: 2025-3-21 23:17
Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery,icroscopy, and diffusion ordered spectroscopy. Moreover, we describe standardized biological tests to evaluate the silencing efficiency by the use of a cell line constitutively expressing the luciferase reporter gene.作者: Plaque 時(shí)間: 2025-3-22 04:04 作者: 開頭 時(shí)間: 2025-3-22 06:44
Layer-by-Layer Assembled Nanoparticles for siRNA Delivery,e the manufacturing of poly(lactic-.-glycolic acid) NPs coated with siRNA for gene silencing. Positively charged polyethyleneimine and negatively charged nucleic acids form the polyelectrolyte shell. Finally, the NPs are functionalized with hyaluronic acid, a polysaccharide which targets the CD44 receptor.作者: 疲勞 時(shí)間: 2025-3-22 12:25 作者: 致命 時(shí)間: 2025-3-22 16:57 作者: Latency 時(shí)間: 2025-3-22 20:30 作者: BARGE 時(shí)間: 2025-3-22 23:16
Book 2019Latest editionnucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealin作者: 濃縮 時(shí)間: 2025-3-23 04:17
Synthesis of Bioreducible Polycations with Controlled Topologies,ations include decreased cytotoxicity due to intracellular reducing environment in the cytoplasm that contains high levels of reducing molecules such as glutathione. Here, we describe the synthesis of bioreducible polycations with emphasis on methods to control their topology.作者: 破布 時(shí)間: 2025-3-23 07:12 作者: milligram 時(shí)間: 2025-3-23 12:16
Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection,tives under aqueous conditions. Here we describe an optimized cleavage protocol (precooling cleavage solution to 4?°C, 20?min cleavage at 22?°C), which minimizes TFA adduct formation, retains the unsaturated hydrocarbon chain character, and ensures high yields of the synthesis.作者: prosperity 時(shí)間: 2025-3-23 16:57 作者: 江湖騙子 時(shí)間: 2025-3-23 19:27
Layer-By-Layer Film Engineering for Sequential Gene Delivery,rescence spectroscopy. Insertion of barrier layers in bioreducible LbL films is necessary to stabilize the interior layer structure and slow down the film degradation rate to achieve sequential gene delivery. Localized gene delivery from the LbL films is demonstrated using human embryonic kidney 293 (HEK 293) cells.作者: 無節(jié)奏 時(shí)間: 2025-3-24 01:40
1064-3745 etailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and establish作者: 無法治愈 時(shí)間: 2025-3-24 03:36
Manfred Ogris,Haider SamiIncludes cutting-edge techniques.Provides step-by-step details essential for reproducible results.Contains key advice from the experts作者: investigate 時(shí)間: 2025-3-24 07:13
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: 托運(yùn) 時(shí)間: 2025-3-24 12:49
Nanotechnology for Nucleic Acid Delivery978-1-4939-9092-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Thyroiditis 時(shí)間: 2025-3-24 17:29
Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formaeprotection, isolation, and purification. We also present methods for oligonucleotides/In. complex formulation with single and double stranded oligonucleotides of RNA nature and give an example of preparation method for one supramolecular drug delivery system (DDS) consisting of radiolabeled siRNA and positively charged peptide.作者: 輕快來事 時(shí)間: 2025-3-24 23:03 作者: Inferior 時(shí)間: 2025-3-25 00:04 作者: 責(zé)任 時(shí)間: 2025-3-25 05:51
https://doi.org/10.1007/978-1-4939-9092-4Delivery systems; Toxicology; Bioconjugates; Nanocarriers; Nanoformulations; Nucleic acid therapies; Bioco作者: Yag-Capsulotomy 時(shí)間: 2025-3-25 10:36
questionable value with France and Britain, Sweden-Norway looked to Denmark to form a barrier against Russia. Meanwhile, Denmark’s relations with the German Confederation deteriorated rapidly as the conflict over the duchies of Schleswig and Holstein—left unresolved by the First Schleswig War—escala作者: ALLEY 時(shí)間: 2025-3-25 13:15
S?ren Reinhard,Ernst Wagnerheral and export-oriented countries with abundant natural resources for producing and exporting goods derived from livestock rearing. Despite these similarities, the three countries experienced different trajectories in terms of income per capita. This chapter examines, from a comparative perspectiv作者: 同謀 時(shí)間: 2025-3-25 17:47 作者: 瑪瑙 時(shí)間: 2025-3-25 23:35
Antoine Kichler,A. James Mason,Arnaud Marquette,Burkhard Bechingerica experienced such different trajectories during the twentieth century. While Scandinavia converged with the rich countries, South America fell behind. This bifurcation occurred even though the two regions developed a similar role as suppliers of natural resources as they were drawn into the emerg作者: shrill 時(shí)間: 2025-3-26 00:24 作者: 證實(shí) 時(shí)間: 2025-3-26 08:07
Maxim Antopolskymovement basis of the Scandinavian voluntary sector model, making it democratically less important, or whether we are witnesses to a reconfiguration of this model. To what degree has the organizations’ integral role in the Scandinavian societies been weakened vertically (links between the local and 作者: 高度贊揚(yáng) 時(shí)間: 2025-3-26 10:43
Wolfgang R?dl,Alexander Taschauer,David Schaffert,Ernst Wagner,Manfred Ogrisd historians present case studies that focus on the scope and impact of Scandinavian colonial expansion in the North, Africa, Asia and America as well as within Scandinavia itsself. They discuss early modern thinking and theories made valid and developed in early modern Scandinavia that justified an作者: Blemish 時(shí)間: 2025-3-26 13:37 作者: 額外的事 時(shí)間: 2025-3-26 17:18
Michel Bessodes,Helene Dhotel,Nathalie Mignetof Scandinavian colonial expansion in the North, Africa, Asi? .??????????????? ??In .Scandinavian Colonialism and the Rise of Modernity: Small Time Agents in a Global Arena., archaeologists, anthropologists, and historians present case studies that focus on the scope and impact of Scandinavian colon作者: PAGAN 時(shí)間: 2025-3-27 00:35
Kaushik Thanki,Xianghui Zeng,Camilla Fogedof Scandinavian colonial expansion in the North, Africa, Asi? .??????????????? ??In .Scandinavian Colonialism and the Rise of Modernity: Small Time Agents in a Global Arena., archaeologists, anthropologists, and historians present case studies that focus on the scope and impact of Scandinavian colon作者: 貝雷帽 時(shí)間: 2025-3-27 03:30 作者: 針葉 時(shí)間: 2025-3-27 08:58 作者: Prosaic 時(shí)間: 2025-3-27 12:04
Angela K. Pannier,Tyler Kozisek,Tatiana Seguraof Scandinavian colonial expansion in the North, Africa, Asi? .??????????????? ??In .Scandinavian Colonialism and the Rise of Modernity: Small Time Agents in a Global Arena., archaeologists, anthropologists, and historians present case studies that focus on the scope and impact of Scandinavian colon作者: 攤位 時(shí)間: 2025-3-27 16:08 作者: 分開如此和諧 時(shí)間: 2025-3-27 20:39
Julia Christina Kasper,Sarah Hedtrich,Wolfgang Friessd historians present case studies that focus on the scope and impact of Scandinavian colonial expansion in the North, Africa, Asia and America as well as within Scandinavia itsself. They discuss early modern thinking and theories made valid and developed in early modern Scandinavia that justified an作者: surrogate 時(shí)間: 2025-3-28 00:11
Katharina Müller,Manfred Ogris,Haider Samid historians present case studies that focus on the scope and impact of Scandinavian colonial expansion in the North, Africa, Asia and America as well as within Scandinavia itsself. They discuss early modern thinking and theories made valid and developed in early modern Scandinavia that justified an作者: legacy 時(shí)間: 2025-3-28 05:04
Heleen Dewitte,Silke Roovers,Stefaan C. De Smedt,Ine Lentackerd historians present case studies that focus on the scope and impact of Scandinavian colonial expansion in the North, Africa, Asia and America as well as within Scandinavia itsself. They discuss early modern thinking and theories made valid and developed in early modern Scandinavia that justified an作者: HUSH 時(shí)間: 2025-3-28 06:59 作者: Mnemonics 時(shí)間: 2025-3-28 12:48
Ladan Parhamifar,Helene Andersen,S. Moein Moghimid historians present case studies that focus on the scope and impact of Scandinavian colonial expansion in the North, Africa, Asia and America as well as within Scandinavia itsself. They discuss early modern thinking and theories made valid and developed in early modern Scandinavia that justified an作者: hemophilia 時(shí)間: 2025-3-28 18:27 作者: 代理人 時(shí)間: 2025-3-28 22:20
Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection,d synthesis. However, the trifluoroacetic acid (TFA)-mediated removal of acid-labile protecting groups and cleavage from the resin can be accompanied by side products caused by an addition of TFA to the double bonds of unsaturated fatty acids. These TFA adducts are converted into hydroxylated deriva作者: amputation 時(shí)間: 2025-3-29 00:33
Synthesis of Bioreducible Polycations with Controlled Topologies, stability in extracellular physiological condition and bioreduction-triggered release of the genetic material. Further benefits of bioreducible polycations include decreased cytotoxicity due to intracellular reducing environment in the cytoplasm that contains high levels of reducing molecules such 作者: 滔滔不絕地講 時(shí)間: 2025-3-29 03:44
Histidine-Rich Cationic Cell-Penetrating Peptides for Plasmid DNA and siRNA Delivery,rfering RNA (siRNA) into mammalian cells. Their ability to bind and protect nucleic acids and then disrupt membranes when activated at low pH enables them to harness the endocytic machinery to deliver cargo efficiently and with low associated toxicity. This chapter describes protocols for the chemic作者: Carcinoma 時(shí)間: 2025-3-29 08:12
Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugration of oligonucleotide conjugates depends critically on the chemical nature of the used ligand and linker, there is no general and universal procedure. Here, we present a detailed, quick, and facile protocol for attaching fluorescent dyes or cross-linkers of variable chemical stability to oligonu作者: Decimate 時(shí)間: 2025-3-29 12:18 作者: JADED 時(shí)間: 2025-3-29 15:53 作者: Musket 時(shí)間: 2025-3-29 21:09
Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery,ained interest as stabilized monodisperse systems showing potential for the delivery of hydrophobic drugs as well as of larger biomolecules such as nucleic acids. Introduction of pH-sensitive histidine groups at the surface of the micellar PDA systems allows for efficient delivery of siRNA resulting作者: 夾死提手勢(shì) 時(shí)間: 2025-3-30 01:14 作者: Incompetent 時(shí)間: 2025-3-30 05:55 作者: Lipoprotein 時(shí)間: 2025-3-30 11:10
Layer-by-Layer Assembled Nanoparticles for siRNA Delivery,layer technique has resulted from its accessibility to every lab; to generate nanoscale structures, layer-by-layer processes require common lab equipment of only modest quality and do not involve the use of organic solvents. In addition, a wide range of different starting materials can be flexibly c作者: Slit-Lamp 時(shí)間: 2025-3-30 12:52 作者: 工作 時(shí)間: 2025-3-30 20:08 作者: 疲憊的老馬 時(shí)間: 2025-3-30 22:39
In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nanofilms,dynamics is key to the understanding and control of gene delivery from nanosystems. Here we describe atomic force microscope (AFM) application to the understanding of DNA release dynamics from bioreducible polycation-based nanosystems. The two nanosystems are polyplex nanoparticles and layer-by-laye作者: Bucket 時(shí)間: 2025-3-31 03:24 作者: impaction 時(shí)間: 2025-3-31 07:18 作者: drusen 時(shí)間: 2025-3-31 11:58
Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles,ne only has to reach a subset of cells in the body. Therefore, targeted delivery of genes to the desired tissue is a major issue in gene delivery. Many different possibilities of targeted gene delivery have been studied. A physical approach to target nucleic acids and other drugs to specific regions作者: CAGE 時(shí)間: 2025-3-31 16:28 作者: 蝕刻術(shù) 時(shí)間: 2025-3-31 19:03
Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity,icity is essential for design and construction of more effective polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput作者: 斑駁 時(shí)間: 2025-4-1 00:22 作者: Digitalis 時(shí)間: 2025-4-1 05:31
,Preparation, Characterization, and In Vitro Evaluation of Lipidoid–Polymer Hybrid Nanoparticles for aqueous buffer is emulsified by sonication with an organic phase containing lipid and polymer into a primary emulsion. Subsequently, the primary emulsion is emulsified with a secondary water phase containing polyvinyl alcohol by sonication, and the organic phase is evaporated, eventually resulting 作者: COLON 時(shí)間: 2025-4-1 08:08
Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles,ffolds (i.e., hydrogel-mediated delivery) to efficiently deliver genes. This chapter includes protocols for surface-mediated DNA delivery focusing on the simplest and most effective methods, which include nonspecific immobilization of DNA complexes (both polymer and lipid vectors) onto serum-coated 作者: anaerobic 時(shí)間: 2025-4-1 13:30 作者: 針葉樹 時(shí)間: 2025-4-1 15:15
Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery,netic responsiveness and ultrasound contrast effects. Protocols are given for the transfection procedure in adherent cells, evaluation of the association of the magnetic vectors with the cells, reporter gene expression analysis, and cell viability assessment.作者: 懲罰 時(shí)間: 2025-4-1 18:47 作者: Agility 時(shí)間: 2025-4-2 02:01 作者: CUR 時(shí)間: 2025-4-2 03:29
