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標(biāo)題: Titlebook: Nanoscale Imaging of Synapses; New Concepts and Opp U. Valentin N?gerl,Antoine Triller Book 2014 Springer Science+Business Media New York 2 [打印本頁]

作者: 誓約    時間: 2025-3-21 19:33
書目名稱Nanoscale Imaging of Synapses影響因子(影響力)




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書目名稱Nanoscale Imaging of Synapses網(wǎng)絡(luò)公開度學(xué)科排名




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書目名稱Nanoscale Imaging of Synapses被引頻次學(xué)科排名




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書目名稱Nanoscale Imaging of Synapses讀者反饋




書目名稱Nanoscale Imaging of Synapses讀者反饋學(xué)科排名





作者: Aqueous-Humor    時間: 2025-3-21 20:39
Flash-and-Freeze Electron Microscopy: Coupling Optogenetics with High-Pressure Freezing,s. We demonstrated that synaptic vesicle fusion intermediates could be captured using this technique. This method can be readily applied to other light-activatable molecules, such as caged compounds, light-switchable ligands, and photoactivatable proteins, to study dynamic changes in cells.
作者: 饒舌的人    時間: 2025-3-22 02:09

作者: seruting    時間: 2025-3-22 06:53
Electron Tomography for the Study of Synaptic Ultrastructure in Fixed Brain Sections,e describe an approach to the study of ultrastructure that relies on plastic-embedded aldehyde-fixed material stabilized with tannic acid instead of osmium tetroxide. This approach offers a new window into the structural basis of synaptic processing in the mammalian brain.
作者: 一再煩擾    時間: 2025-3-22 11:26
Book 2014ion in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.
作者: labile    時間: 2025-3-22 13:21
Book 2014ease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even dow
作者: absolve    時間: 2025-3-22 18:23

作者: 不真    時間: 2025-3-22 21:16
Nanoscale Imaging of Synapses978-1-4614-9179-8Series ISSN 0893-2336 Series E-ISSN 1940-6045
作者: 確定無疑    時間: 2025-3-23 03:12

作者: 控制    時間: 2025-3-23 09:10

作者: mercenary    時間: 2025-3-23 10:50
https://doi.org/10.1007/978-1-4614-9179-8live-cell fluorescence microsopy; nanoscopy; neurons and glia; physiology; synapses
作者: 傻瓜    時間: 2025-3-23 16:06
Nanoscale Imaging of Protein Molecules at the Postsynaptic Density,ld EM coupled with tomography identifies a class of membrane-associated vertical filaments in the PSD core as PSD-95, the major scaffolding protein. Acute knockdown of PSD-95 with RNAi leads to loss of patches of electron-dense PSD material, including loss of vertical filaments and their associated
作者: 饑荒    時間: 2025-3-23 18:04

作者: glans-penis    時間: 2025-3-23 22:45
Live-Cell PALM of Intracellular Proteins in Neurons,vantages over other imaging modalities. First, it permits the localization of single molecules with precision on the order of tens of nanometers and the rendering of neuronal structures at high resolution. As this technique utilizes expressed proteins tagged with photoconvertible fluorescent protein
作者: forager    時間: 2025-3-24 05:43

作者: esculent    時間: 2025-3-24 08:00

作者: delusion    時間: 2025-3-24 13:52
Imaging-Based Measures of Synaptic Tenacity,ar dynamics of synaptic components and on quantitative measures of synaptic tenacity—the capacity of synapses to maintain their characteristics over time. These include fluorescence recovery after photobleaching (FRAP), fluorescence recovery after photoactivation (FRAPA), and several analytical tool
作者: MORPH    時間: 2025-3-24 16:43
Zooming in on the (Peri)synaptic Extracellular Matrix,artite system including the pre- and postsynapse, associated astroglial terminals, and the ECM, in which there is an exchange of signals between all four components. This review is to give a short introduction to neural ECM; to summarize available electron microscopy data on expression of perisynapt
作者: 膽小鬼    時間: 2025-3-24 20:26
Two-Photon Excitation STED Microscopy for Imaging Synapses and Glia in Acute Brain Slices,with a powerful array of complementary techniques, such as photo-uncaging, fluorescence recovery after photobleaching (FRAP), and patch-clamp electrophysiology, giving experimental access to a rich set of molecular, structural, and functional data..Here, we present a primer on two-photon excitation
作者: LIMN    時間: 2025-3-25 02:35
0893-2336 he various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.978-1-4939-4918-2978-1-4614-9179-8Series ISSN 0893-2336 Series E-ISSN 1940-6045
作者: Choreography    時間: 2025-3-25 06:40
Svetlana Korotchenko,Francesca Cella Zanacchi,Alberto Diaspro,Alexander Dityatev
作者: induct    時間: 2025-3-25 10:53

作者: foreign    時間: 2025-3-25 13:42

作者: 闡釋    時間: 2025-3-25 15:58

作者: collateral    時間: 2025-3-25 20:31
Shigeki Watanabe,M. Wayne Davis,Erik M. Jorgensen geschichtlichen Entwicklung eine Vielzahl von Versuchen voraus, die Interessen des Eigentümers und die der Verkehrssicherheit zufriedenstellend zu regeln. Gegen die M?glichkeit des gutgl?ubigen Erwerbs kann man nicht geltend machen, sie versto?e gegen die Logik, weil niemand etwas geben k?nne, was
作者: Stagger    時間: 2025-3-26 03:05

作者: collateral    時間: 2025-3-26 05:28
Kaiyu Zheng,James A. Levitt,Klaus Suhling,Dmitri A. Rusakov Aufstellung eines allgemeinen Teils für alle Pfandrechte zwar für m?glich, doch meinte man, da? damit die Regelung weder übersichtlicher noch brauchbarer werdet. Statt dessen regelte man zun?chst die Hypothek und verwies bei der Grundschuld unter Hervorhebung der Abweichungen hierauf, § 1192 I; für
作者: gusher    時間: 2025-3-26 10:10

作者: 冰雹    時間: 2025-3-26 13:22

作者: 惰性氣體    時間: 2025-3-26 19:54
Alain C. Burette,Richard J. Weinbergbesitzt, herausverlangen: Vindikationsprinzip. Dieser Anspruch macht die St?rke des Eigentums aus, gef?hrdet aber auf der anderen Seite die Sicherheit des Rechtsverkehrs: Wer eine Sache erworben hat, mu? jederzeit damit rechnen, da? ein Dritter sich als Eigentümer legitimiert und ihm die Sache wegni
作者: PRO    時間: 2025-3-26 21:21
Shigeki Watanabe,M. Wayne Davis,Erik M. Jorgensenbesitzt, herausverlangen: Vindikationsprinzip. Dieser Anspruch macht die St?rke des Eigentums aus, gef?hrdet aber auf der anderen Seite die Sicherheit des Rechtsverkehrs: Wer eine Sache erworben hat, mu? jederzeit damit rechnen, da? ein Dritter sich als Eigentümer legitimiert und ihm die Sache wegni
作者: concubine    時間: 2025-3-27 01:58
Nicholas A. Frost,Harold D. MacGillavry,Hsiangmin E. Lu,Thomas A. BlanpiedJohows Vorentwurf kannte nur die Hypothek, die erste Kommission schuf in Anlehnung an das preu?ische Recht die nicht akzessorische Grundschuld und fa?te das Mobiliarpfand, die Hypothek und die Grundschuld im 9. Abschnitt unter der überschrift ?Pfandrecht und Grundschuld“ zusammen.. Dabei war man sic
作者: 嬉耍    時間: 2025-3-27 05:37

作者: 健談    時間: 2025-3-27 11:17
Nanoscale Imaging of Protein Molecules at the Postsynaptic Density,smission, signal transduction, and processing and provides a substrate for synaptic plasticity. Here we describe approaches to unravel the molecular organization of the PSD to gain insights into its synaptic function. Dissociated cultures of rat hippocampal neurons are prepared by high-pressure free
作者: 蒙太奇    時間: 2025-3-27 16:49

作者: 接合    時間: 2025-3-27 20:18
Flash-and-Freeze Electron Microscopy: Coupling Optogenetics with High-Pressure Freezing, method to combine optogenetics with electron microscopy to capture dynamic changes in synaptic morphology during neurotransmission. First we generated transgenic . animals expressing channelrhodopsin in specific neurons. We stimulated these neurons in intact animals using a home-built light stimula
作者: Intuitive    時間: 2025-3-28 01:17

作者: Diskectomy    時間: 2025-3-28 04:40
Visualizing the Ultrastructures and Dynamics of Synapses by Single-Molecule Nanoscopy,ng these super-resolution techniques, photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) are based on the detection of single fluorophores, whose signals are localized with great precision. The strength of single-molecule approaches lies in their h
作者: PAEAN    時間: 2025-3-28 09:39
Live-Cell PALM of Intracellular Proteins in Neurons,lecules critical to synaptic maintenance and plasticity. The small spatial scales of the synapse and the dendritic spines which house the postsynaptic machinery have limited the direct visualization of their structure in living cells. A number of imaging methods have evolved in recent years with the
作者: Aerate    時間: 2025-3-28 11:00
Monitoring Nanoscale Mobility of Small Molecules in Organized Brain Tissue with Time-Resolved Fluorof the brain. How fast such molecules can move in the crowded protein environment of live issue is, however, poorly understood. To enable the monitoring of molecular mobility on the nanoscale in situ, we have combined two-photon excitation microscopy and patch-clamp electrophysiology with time-resol
作者: Lament    時間: 2025-3-28 15:47
Imaging Signaling Transduction in Single Dendritic Spines,eds of protein species. As synaptic plasticity is caused by signaling coordinated differentially in space and time, understanding how the various proteins differ in their spatial and temporal signaling profiles is key to our understanding of synaptic plasticity as a whole. However, acquiring such in
作者: Brain-Imaging    時間: 2025-3-28 19:57
Imaging-Based Measures of Synaptic Tenacity,tional properties of neuronal networks, possibly providing the basis for phenomena collectively referred to as “l(fā)earning and memory.” This belief has an important corollary: It implies that synapses, when . driven to change their characteristics by physiologically relevant stimuli, should retain the
作者: CRACK    時間: 2025-3-28 23:17
Zooming in on the (Peri)synaptic Extracellular Matrix,e diverse forms of the ECM. The most conspicuous aggregates of ECM molecules, containing chondroitin sulfate proteoglycans, hyaluronic acid, link proteins, and tenascin-R, are found around perisomatic GABAergic synapses on fast-spiking interneurons. However, also the perisynaptic extracellular space
作者: Demulcent    時間: 2025-3-29 03:39
Two-Photon Excitation STED Microscopy for Imaging Synapses and Glia in Acute Brain Slices,f living cells with a spatial resolution well below 100 nm, even down to a few nanometers. By breaking the classical diffraction barrier of optical microscopy, it offers the chance to close the gap in spatial resolution between electron microscopy and conventional light microscopy and is attracting
作者: palpitate    時間: 2025-3-29 11:16
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