作者: effrontery 時(shí)間: 2025-3-22 00:02
Fidan Sumbul,Arin Marchesi,Hirohide Takahashi,Simon Scheuring,Felix Ricocoastline regions. Use of open canals to transport waste water and use of evaporation ponds to reduce waste water volume are standard engineering practices. Engineered designs tend to focus on practicality and efficiency without regard to principles of landscape ecology. The concept of a saline ecos作者: 杠桿支點(diǎn) 時(shí)間: 2025-3-22 02:02 作者: harpsichord 時(shí)間: 2025-3-22 08:01 作者: pessimism 時(shí)間: 2025-3-22 12:25
High-Resolution Atomic Force Microscopy Imaging of Nucleic Acids has beaten the barrier of resolution continuously, thanks to technical developments, miniaturization of tips, and implementation of new imaging modes. The double helix structure of DNA has been always at the horizon of resolution. Today, this milestone has been reached, not only imaging DNA but als作者: 圓錐 時(shí)間: 2025-3-22 15:03
Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensingwith controversial results. Here, we designed molecular tools to measure the interaction forces between the serotonin transporter (SERT) and S-citalopram on the single-molecule level by means of atomic force microscopy. Force spectroscopy allows for the extraction of dynamic information under physio作者: 天真 時(shí)間: 2025-3-22 19:42
AFM-Based Single-Molecule Force Spectroscopy of Proteinsand manipulating the mechanical properties of single molecules. The past 20?years of AFM-SMFS has provided numerous breakthroughs in the understanding of the mechanical properties and force-induced structural rearrangements of sugars, DNA, and proteins. Here, we focus on the application of AFM-SMFS 作者: 相容 時(shí)間: 2025-3-22 22:59 作者: 痛打 時(shí)間: 2025-3-23 05:18 作者: 小臼 時(shí)間: 2025-3-23 07:27 作者: 絕緣 時(shí)間: 2025-3-23 10:14 作者: PACT 時(shí)間: 2025-3-23 15:56
Supported Lipid Bilayers for Atomic Force Microscopy Studieslipid bilayer (SLB), which itself is a traditional model for cellular membranes. Success in these studies is based on the availability of a stable SLB for the required observation period, which can extend several hours. The application of AFM requires that the SLB have a smooth morphology, thus enab作者: Asseverate 時(shí)間: 2025-3-23 20:27 作者: Graduated 時(shí)間: 2025-3-24 01:35
Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy conditions. One of the key factors leading to successful HS-AFM observations is the selection of an appropriate substrate depending on molecules to be observed. For the HS-AFM imaging, a target molecule must be absorbed on a substrate by controlling its orientation without impairing the dynamics or作者: AGONY 時(shí)間: 2025-3-24 05:47 作者: membrane 時(shí)間: 2025-3-24 10:31 作者: CHURL 時(shí)間: 2025-3-24 14:32 作者: Chauvinistic 時(shí)間: 2025-3-24 17:28
Assembly of Centromere Chromatin for Characterization by High-Speed Time-Lapse Atomic Force Microscoging in ambient conditions can provide details of the conformational states and interactions of a population of molecules which is well complemented by single-molecule imaging of the systems dynamics using time-lapse AFM imaging, in which images are capture at rates of 10–15 frames per second in an 作者: Blatant 時(shí)間: 2025-3-24 19:37 作者: Fierce 時(shí)間: 2025-3-25 01:40
Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assayswith high spatiotemporal resolution. However, it remains challenging to obtain functional eukaryotic protein complexes and cost-effective fluorescently labeled RNAs to study their interactions at the single-molecule level. Here, we describe protocols combining single-molecule fluorescence with vario作者: 你不公正 時(shí)間: 2025-3-25 03:57 作者: 使激動(dòng) 時(shí)間: 2025-3-25 10:27 作者: 賞錢(qián) 時(shí)間: 2025-3-25 11:48
Yuri L. LyubchenkoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: exhilaration 時(shí)間: 2025-3-25 18:27
978-1-4939-9336-9Springer Science+Business Media, LLC, part of Springer Nature 2018作者: LATHE 時(shí)間: 2025-3-25 22:27 作者: MANIA 時(shí)間: 2025-3-26 02:05
High-Resolution Atomic Force Microscopy Imaging of Nucleic Acidso its close relative double-stranded RNA. Here, we provide a comprehensive description of the methods employed and the steps required to image the helical periodicity of these two nucleic acids with the sample immersed in a buffer solution.作者: 孤僻 時(shí)間: 2025-3-26 06:04
Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy physiological function of the molecule. In this chapter, we describe protocols for preparation of substrates that have been used for HS-AFM and then introduce observation examples on dynamic processes of biological molecules.作者: 彩色的蠟筆 時(shí)間: 2025-3-26 11:16
High-Resolution and High-Speed Atomic Force Microscope Imagingt only a few labs in the world had access to (including ours) to an established commercialized technology that is present in tens of labs around the world. In this protocol chapter we share with the readers our practical know-how on high resolution HS-AFM imaging.作者: 慢慢啃 時(shí)間: 2025-3-26 16:25 作者: rheumatism 時(shí)間: 2025-3-26 18:41
High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assembliesctural dynamics of relevant biomolecules for various biological phenomena. Here, we describe the methods for HS-AFM observation and the analysis of the structural dynamics of individual amyloidogenic protein assemblies using amyloid β 1–42 as an example.作者: 殘暴 時(shí)間: 2025-3-27 00:26
1064-3745 ation advice from the experts.This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules i作者: 最高峰 時(shí)間: 2025-3-27 04:58
Book 2018cessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and through, .Nanoscale Imaging: Methods and Protocols. is a valuable resource for anyone interested in learning more about this developing and expanding field..作者: 賞心悅目 時(shí)間: 2025-3-27 07:56 作者: semiskilled 時(shí)間: 2025-3-27 10:26
Supported Lipid Bilayers for Atomic Force Microscopy Studiesefect-free POPC and POPS SLBs that remain stable for at least 8?h. The application of such smooth and stable surfaces is illustrated by monitoring of the on-surface aggregation of amyloid proteins with the use of time-lapse AFM.作者: Nonconformist 時(shí)間: 2025-3-27 14:32
Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assaysf the utility of our single-molecule approaches, we show how human Dicer and its cofactor TRBP orchestrate the biogenesis of microRNA in real time. These single-molecule pull-down and fluorescence assays provide sub-second time resolution and can be applied to various ribonucleoprotein complexes that are essential for cellular processes.作者: Ornithologist 時(shí)間: 2025-3-27 17:54
AFM-Based Single-Molecule Force Spectroscopy of Proteinsng. In this chapter we describe the experimental procedures for conducting a SMFS-AFM experiment—including purification of protein samples, setup and calibration of the AFM instrumentation, and the thorough and unbiased analysis of resulting AFM data.作者: 割公牛膨脹 時(shí)間: 2025-3-27 22:06
High-Resolution AFM-Based Force Spectroscopyain high force precision at enhanced bandwidths. Both modification methods allow site-specific attachment of biomolecules onto the apex area of the tips for force spectroscopy. The chapter concludes with a comparative demonstration using the two cantilever modification methods to study a lipid-protein interaction.作者: 有毒 時(shí)間: 2025-3-28 05:04
AFM-Based Characterization of Electrical Properties of Materialsce gradients are explained. The applications of these techniques are illustrated on metals, surfactant compounds, semiconductors, and different polymers. Practical recommendations on use of the AFM-based electrical methods and the related challenges are given in the last section.作者: 虛度 時(shí)間: 2025-3-28 07:01
Quantifying Small Molecule Binding Interactions with DNA Nanostructuresle stranded DNA fragments, and arbitrary small molecules. The described methods include microscale thermophoresis, ligand completion assays, circular dichroism spectroscopy, and AutoDock simulations. The protocols use organophosphates and model chemical nerve agents as examples, but the methods described here are broadly applicable.作者: Promotion 時(shí)間: 2025-3-28 11:04 作者: Esalate 時(shí)間: 2025-3-28 15:09 作者: 云狀 時(shí)間: 2025-3-28 22:45 作者: VERT 時(shí)間: 2025-3-28 23:21 作者: SEED 時(shí)間: 2025-3-29 05:14
ecular techniques which can be found in the ever increasing numbers of "cloning manuals. " By necessity, much of what is described in this volume concentrates on one particular species of Saccharomyces, namely Saccharomyces cerevisiae. This is not just a reflection of the authors‘ interests, but indicates the978-1-4899-2643-2978-1-4899-2641-8作者: keloid 時(shí)間: 2025-3-29 08:34 作者: Magnitude 時(shí)間: 2025-3-29 13:03 作者: 拋物線 時(shí)間: 2025-3-29 16:16
Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezersd interactions of DNA and RNA. Here, we provide step-by-step protocols to carry out FOMT and MTT measurements. In particular, we focus on multiplexed measurements, i.e., measurements that record data for multiple nucleic acid tethers at the same time, to improve statistics and to facilitate the obse作者: 領(lǐng)帶 時(shí)間: 2025-3-29 22:54 作者: 木質(zhì) 時(shí)間: 2025-3-30 03:15 作者: 玷污 時(shí)間: 2025-3-30 07:45
Pablo Ares,Julio Gomez-Herrero,Fernando Moreno-Herrero作者: 廢止 時(shí)間: 2025-3-30 11:37 作者: diabetes 時(shí)間: 2025-3-30 14:31
Sibaprasad Maity,Ekaterina Viazovkina,Alexander Gall,Yuri L. Lyubchenko作者: faction 時(shí)間: 2025-3-30 18:06 作者: BOAST 時(shí)間: 2025-3-31 00:04
Zhengjian Lv,Siddhartha Banerjee,Karen Zagorski,Yuri L. Lyubchenko作者: 盡管 時(shí)間: 2025-3-31 03:40
Takayuki Uchihashi,Hiroki Watanabe,Noriyuki Kodera作者: emission 時(shí)間: 2025-3-31 07:48
