標題: Titlebook: NF-κB Transcription Factors; Methods and Protocol Guido Franzoso,Francesca Zazzeroni Book 2021 Springer Science+Business Media, LLC, part o [打印本頁] 作者: 選民 時間: 2025-3-21 16:38
書目名稱NF-κB Transcription Factors影響因子(影響力)
書目名稱NF-κB Transcription Factors影響因子(影響力)學科排名
書目名稱NF-κB Transcription Factors網(wǎng)絡公開度
書目名稱NF-κB Transcription Factors網(wǎng)絡公開度學科排名
書目名稱NF-κB Transcription Factors被引頻次
書目名稱NF-κB Transcription Factors被引頻次學科排名
書目名稱NF-κB Transcription Factors年度引用
書目名稱NF-κB Transcription Factors年度引用學科排名
書目名稱NF-κB Transcription Factors讀者反饋
書目名稱NF-κB Transcription Factors讀者反饋學科排名
作者: 不可侵犯 時間: 2025-3-21 21:32 作者: exceptional 時間: 2025-3-22 03:23 作者: Pruritus 時間: 2025-3-22 04:39 作者: BILE 時間: 2025-3-22 12:37 作者: 步兵 時間: 2025-3-22 15:15
Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionatiing their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.作者: 芭蕾舞女演員 時間: 2025-3-22 18:25 作者: 有效 時間: 2025-3-22 23:34
Methods for Modulating the Pathway of NF-κB Using Short Hairpin RNA (ShRNA)lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect primary cell cultures.作者: 大暴雨 時間: 2025-3-23 04:22 作者: cardiovascular 時間: 2025-3-23 06:54
Measuring NF-κB Phosphorylation and Acetylationcontrol the transcriptional outcomes of this important transcription factor. These modifications work independently, sequentially or in combination to modulate the diverse biological functions of NF-κB in cancer and inflammatory response. Here, we describe some experimental methods to detect the in 作者: 吸引人的花招 時間: 2025-3-23 12:35
Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionatiing their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.作者: Carcinoma 時間: 2025-3-23 16:44
Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proter molecules. This technique allows a snapshot of the structure of tissue and determines the cellular and subcellular localization of a target antigen. This chapter describes how to identify and localize NF-κB proteins in human tissue using immunohistochemical staining on formalin-fixed paraffin-emb作者: orthodox 時間: 2025-3-23 18:35 作者: 主動 時間: 2025-3-23 22:56
Molecular and Biochemical Approaches to Study the Evolution of NF-κB Signaling in Basal Metazoansexample, in members of the phyla Cnidaria (e.g., sea anemones, corals, hydra, jellyfish) and Porifera (sponges), and in several single-celled protists (e.g., ., some choanoflagellates). Therefore, methods are required to study the function of NF-κB and its pathway members in early branching organism作者: Aerophagia 時間: 2025-3-24 04:03
Methods for Modulating the Pathway of NF-κB Using Short Hairpin RNA (ShRNA)lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect 作者: LVAD360 時間: 2025-3-24 08:52 作者: 經(jīng)典 時間: 2025-3-24 10:42
Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-κB Activation in Jurkat T Cellsor (TCR)/CD28 co-stimulation. The critical function of the key regulators identified in Jurkat T cells has subsequently been verified in primary murine and human T cells. CRISPR/Cas9-mediated genomic editing techniques in combination with viral reconstitution are powerful tools that now enable the i作者: sclera 時間: 2025-3-24 15:29 作者: exacerbate 時間: 2025-3-24 22:22 作者: Hyperalgesia 時間: 2025-3-25 01:49 作者: objection 時間: 2025-3-25 04:24
Analysis of the Contribution of NF-κB in the Regulation of Chemotherapy-Induced Cell Senescence by Echemotherapy with genotoxic drugs, both in cancer cells and in normal cells, whose activation critically affects the outcome of cancer therapy. Drug-induced senescent cells undergo a permanent cell cycle arrest, acquire distinctive morphological and biochemical alterations, and an enhanced secretory作者: 泛濫 時間: 2025-3-25 10:52 作者: 歌劇等 時間: 2025-3-25 12:29 作者: predict 時間: 2025-3-25 16:23
Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-κB Signaling in Hypoxia and NormoxiaHIF and NF-κB. To investigate transcription factor function, an important aspect is its ability to bind DNA. The most appropriate method to study this property in cells is the use of chromatin immunoprecipitation followed by qPCR and/or next generation sequencing. This allows identification of poten作者: 無能力之人 時間: 2025-3-25 22:41 作者: Neutral-Spine 時間: 2025-3-26 02:25
Nikita Tushar Modi,Lin-Feng Chenh ‘broader criminological thought’ to enrich analyses of ser.Between 1975 and 1980, Peter Sutcliffe, who became known as the Yorkshire Ripper, murdered 13 women in the North of England. The murders provoked widespread fear amongst women and impacted the public consciousness at both the local and nat作者: 商業(yè)上 時間: 2025-3-26 06:03
Davide Vecchiotti,Daniela Verzella,Daria Capece,Mauro Di Vito Nolfi,Barbara Di Francesco,Jessica Corh ‘broader criminological thought’ to enrich analyses of ser.Between 1975 and 1980, Peter Sutcliffe, who became known as the Yorkshire Ripper, murdered 13 women in the North of England. The murders provoked widespread fear amongst women and impacted the public consciousness at both the local and nat作者: Etching 時間: 2025-3-26 11:42
Pablo J. Aguirre Carrión,Leah M. Williams,Thomas D. Gilmorerural areas of Hungary.Overview of the most important local With the transformation of the role of the welfare state, the “social engagement” and “active participation” were taking the place of passive benefits. The importance of individual actions is becoming more and more prominent..With the reduc作者: 沒有希望 時間: 2025-3-26 13:51 作者: 過份好問 時間: 2025-3-26 18:21 作者: 不透明 時間: 2025-3-26 21:20
Eugene Varfolomeev,Tatiana Goncharov,Domagoj Vucicbaus im Hinblick auf deren allt?gliche Auseinandersetzung mit dem Einflüssen des Bergbaus. Zu den gravierendsten z?hlt die Devastierung von Ortschaften, in deren Vorfeld die Einwohner an andere Wohnstandorte umgesiedelt wurden. Die vielschichtige Problematik bergbaubedingter Umsiedlungen infolge der作者: LUCY 時間: 2025-3-27 04:47 作者: FILTH 時間: 2025-3-27 07:05 作者: 哺乳動物 時間: 2025-3-27 13:20 作者: 態(tài)度暖昧 時間: 2025-3-27 14:23 作者: Defraud 時間: 2025-3-27 20:03
. In Islam, land was the first waqf, which has been randomly used in different countries of the world, including Bangladesh. But, according to 1986 census of waqf estate, there are thousands of waqf estates in Bangladesh which are neglected and sloppily maintained, underdeveloped and are devoid of a作者: CYN 時間: 2025-3-27 23:23
Francesco Pacifico,Elvira Crescenzi,Antonio Leonardis of Islamic banks using the waqf. The data for the study was collected through a self-administered questionnaire from 317 randomly selected depositors of two Islamic banks. The structural equation modelling approach was used to establish the significant determinants and validate the Modified Key Me作者: 頭盔 時間: 2025-3-28 03:25 作者: commodity 時間: 2025-3-28 07:08
Alessandra Pescatore,Carmela Casale,Francesca Fusco,Matilde Valeria Ursinily during contemporary times. The paper assesses the latent status of waqf by examining the legal constraints that waqf faces relative to other non-profit organizations. While Shariah principles and national laws affect the nature of waqf in contemporary nation states, the latter ultimately determin作者: Outshine 時間: 2025-3-28 11:45
Dilem Shakir,Michael Batie,Sonia Rochaly during contemporary times. The paper assesses the latent status of waqf by examining the legal constraints that waqf faces relative to other non-profit organizations. While Shariah principles and national laws affect the nature of waqf in contemporary nation states, the latter ultimately determin作者: 憤慨一下 時間: 2025-3-28 16:11
Gaurav Swarnkar,Manoj Arra,Suresh Adapala,Yousef Abu-Amers of Islamic banks using the waqf. The data for the study was collected through a self-administered questionnaire from 317 randomly selected depositors of two Islamic banks. The structural equation modelling approach was used to establish the significant determinants and validate the Modified Key Me作者: 駁船 時間: 2025-3-28 18:46
Book 2021provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pathways of NF-κB activation and contextualize them within organismal physiology and disease pathogenesis, using genetic and biochemical techniques and some of 作者: Blazon 時間: 2025-3-29 02:20
Methods to Study the Effect of IKK Inhibition on TNF-Inducing Apoptosis and Necroptosis in Cultured armacological interventions with NF-kappaB and death inhibitors. We provide protocol to detect death-specific proteins using immunoblot and to dissect necrosome complex by sequential co-immunoprecipitation of death-specific components during necroptosis.作者: MELON 時間: 2025-3-29 03:18
Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-κB Signaling in Hypoxia and Normoxia property in cells is the use of chromatin immunoprecipitation followed by qPCR and/or next generation sequencing. This allows identification of potentially directly regulated genes as well as enhancer regions. Here we describe the ChIP-qPCR method in detail, including key aspects important for the success of the technique.作者: 松馳 時間: 2025-3-29 08:55 作者: Little 時間: 2025-3-29 12:59 作者: 演講 時間: 2025-3-29 19:26 作者: Foment 時間: 2025-3-29 23:33
Analysis of the Contribution of NF-κB in the Regulation of Chemotherapy-Induced Cell Senescence by Estem for the investigation of the role of NF-κB in TIS. We also describe protocols routinely used in our laboratory, to investigate TIS in this Tet-On inducible expression system. Finally, we describe techniques for the validation of TIS induction.作者: cluster 時間: 2025-3-30 00:02
1064-3745 expertsThis detailed book serves as a systematic examination of the analytical methods to study the transcription factor NF-κB in physiology and disease. It provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pa作者: NOMAD 時間: 2025-3-30 06:41 作者: 創(chuàng)造性 時間: 2025-3-30 11:52
High-Throughput Analysis of the Cell and DNA Site-Specific Binding of Native NF-κB Dimers Using Nuclcharacterize the DNA binding of transcription factors present in cell nuclear extracts. NextPBMs allow for sensitive analysis of the DNA binding of NF-κB dimers and their interactions with cell-specific cofactors.作者: Disk199 時間: 2025-3-30 13:16
1064-3745 ratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .NF-κB Transcription Factors: Methods and Protocols. 978-1-0716-1671-0978-1-0716-1669-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Free-Radical 時間: 2025-3-30 18:09 作者: bleach 時間: 2025-3-31 00:10
Heather Hook,Rose W. Zhao,David Bray,Jessica L. Keenan,Trevor Siggers作者: 油氈 時間: 2025-3-31 03:51
Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-κB Activation in Jurkat T Cellsiral transduction protocol. In addition, we explain the use of a stable NF-κB-dependent EGFP reporter system that enables a reliable quantification of NF-κB transcriptional activation in the reconstituted KO Jurkat T cells.作者: 充滿裝飾 時間: 2025-3-31 08:57
Analysis of Calcium Control of Canonical NF-κB Signaling in B Lymphocytescells (NFAT). NFAT is expressed ubiquitously and the mechanism by which Ca. regulates NFAT activation and signaling is well known. By contrast, a mechanistic understanding of how Ca. signals also shape the activation and specificity of NF-κB to control the expression of pro-inflammatory genes has la作者: immunity 時間: 2025-3-31 09:45
