標(biāo)題: Titlebook: Microtubules; Methods and Protocol Hiroshi Inaba Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri [打印本頁(yè)] 作者: concession 時(shí)間: 2025-3-21 20:04
書(shū)目名稱(chēng)Microtubules影響因子(影響力)
作者: 口音在加重 時(shí)間: 2025-3-21 21:16 作者: 紅潤(rùn) 時(shí)間: 2025-3-22 02:14 作者: Gourmet 時(shí)間: 2025-3-22 07:43 作者: 向下 時(shí)間: 2025-3-22 11:14
Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay Usinnterference Contrast (FLIC) microscopy can illuminate the height of the microtubule above a surface, which, at sufficiently low surface densities of kinesin, also reveals the number, locations, and dynamics of the bound motors.作者: heterogeneous 時(shí)間: 2025-3-22 14:31
Assembling Microtubule-Based Active Matterls and associated isotropic fluids. We describe the purification of each protein and the assembly process of a two-dimensional active nematic on a water–oil interface. Finally, we show examples of nematic formation and describe methods for quantifying their non-equilibrium dynamics.作者: 可以任性 時(shí)間: 2025-3-22 18:40
Book 2022ections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules, comprehensive approaches to analyze properties of microtubules, and functionalization of microtubul作者: CLASP 時(shí)間: 2025-3-23 00:50
Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modificationis required. Additionally, fluorescently labeled tubulin has become a powerful approach for extensively studying the dynamics of these components. In this chapter, the process of purifying the heterodimeric αβ-tubulin from porcine brain will be described, as well as the process of labeling of the purified tubulin with fluorescence dye.作者: 有偏見(jiàn) 時(shí)間: 2025-3-23 02:11
Construction of Molecular Robots from Microtubules for Programmable Swarming of molecular robots through conjugation of DNA to MTs and demonstration of swarming of the MTs. The swarming is mediated by DNA-based interaction and photoirradiation which act as processors and sensors respectively for the robots. Furthermore, the required protocols to utilize the swarming of MTs for molecular computation is also described.作者: 燕麥 時(shí)間: 2025-3-23 07:58 作者: 功多汁水 時(shí)間: 2025-3-23 11:45
1064-3745 ation advice from the experts.This volume provides state-of-the-art methodology to functionalize microtubules and construct microtubule-based materials. Chapters consists of six sections detailing modification of microtubule structures, observation and control of microtubule movement, material appli作者: intuition 時(shí)間: 2025-3-23 16:10
1064-3745 mation for construction of microtubule-based materials and help to create new ideas for the next-generation of materials and applications through detailed protocols..978-1-0716-1985-8978-1-0716-1983-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 幸福愉悅感 時(shí)間: 2025-3-23 19:04 作者: 細(xì)微的差異 時(shí)間: 2025-3-24 01:27
Satyajit Ghosh,Shubham Garg,Nabanita Mukherjee,Surajit Ghosh作者: 遺傳學(xué) 時(shí)間: 2025-3-24 05:46
Book 2022ng known pitfalls...?..Authoritative and cutting-edge, .Microtubules: Methods and Protocols .aims to provide useful information for construction of microtubule-based materials and help to create new ideas for the next-generation of materials and applications through detailed protocols..作者: 全國(guó)性 時(shí)間: 2025-3-24 08:33 作者: tackle 時(shí)間: 2025-3-24 14:00 作者: Volatile-Oils 時(shí)間: 2025-3-24 16:13 作者: peptic-ulcer 時(shí)間: 2025-3-24 22:06 作者: Generosity 時(shí)間: 2025-3-25 00:50 作者: 自制 時(shí)間: 2025-3-25 06:30
Kazuya Fujimoto,Ryota Iino,Ryuji Yokokawaulation. ICG angiography is useful in the diagnosis and management of occult choroidal neovascularization in age-related macular degeneration and may be used in other inflammatory conditions with central serous chorioretinopathy. ICG angiography offers advanced imaging for improved monitoring and tr作者: SUE 時(shí)間: 2025-3-25 10:02 作者: 友好關(guān)系 時(shí)間: 2025-3-25 14:47
In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Refleence microscope at low cost and can be combined with fluorescence techniques like total-internal-reflection-fluorescence (TIRF) microscopy. Here we describe the experimental procedure to image microtubule dynamics and severing using IRM, providing practical tips and guidelines to resolve possible ex作者: Generalize 時(shí)間: 2025-3-25 18:45 作者: OASIS 時(shí)間: 2025-3-25 22:49
Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-M as an expansion of zero-mode waveguides (ZMWs). Conventional ZMWs equip apertures with a diameter smaller than the wavelength of light to suppress background noise from the labeled molecules diffusing outside of the apertures. While they are not compatible with filamentous objects, our linear-ZMWs 作者: transient-pain 時(shí)間: 2025-3-26 02:41
Xiao Hu,Cerasela Zoica Dinupproach to the can- to treatment. We hope that in subsequent editions we cer patient; state of the art retinoblastoma treatment will be able to continue to provide updates on such can only be conceived in the context of the multidis- exciting subjects.978-1-4899-7753-3978-0-387-89072-2Series ISSN 1613-5318 Series E-ISSN 2191-0812 作者: TOM 時(shí)間: 2025-3-26 04:47
Alexandra M. Tayar,Linnea M. Lemma,Zvonimir Dogicpproach to the can- to treatment. We hope that in subsequent editions we cer patient; state of the art retinoblastoma treatment will be able to continue to provide updates on such can only be conceived in the context of the multidis- exciting subjects.978-1-4899-7753-3978-0-387-89072-2Series ISSN 1613-5318 Series E-ISSN 2191-0812 作者: Ossification 時(shí)間: 2025-3-26 10:38 作者: fallible 時(shí)間: 2025-3-26 15:33
Jakia Jannat Keya,Arif Md. Rashedul Kabir,Mousumi Akter,Akira Kakugopproach to the can- to treatment. We hope that in subsequent editions we cer patient; state of the art retinoblastoma treatment will be able to continue to provide updates on such can only be conceived in the context of the multidis- exciting subjects.978-1-4899-7753-3978-0-387-89072-2Series ISSN 1613-5318 Series E-ISSN 2191-0812 作者: 搖曳 時(shí)間: 2025-3-26 17:39 作者: motivate 時(shí)間: 2025-3-26 23:42 作者: Occlusion 時(shí)間: 2025-3-27 02:23
Jakia Jannat Keya,Mousumi Akter,Arif?Md.?Rashedul Kabir,Satsuki Ishii,Akira Kakugopproach to the can- to treatment. We hope that in subsequent editions we cer patient; state of the art retinoblastoma treatment will be able to continue to provide updates on such can only be conceived in the context of the multidis- exciting subjects.978-1-4899-7753-3978-0-387-89072-2Series ISSN 1613-5318 Series E-ISSN 2191-0812 作者: 發(fā)現(xiàn) 時(shí)間: 2025-3-27 07:02
Hiroshi InabaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 良心 時(shí)間: 2025-3-27 12:07 作者: GRIN 時(shí)間: 2025-3-27 15:14 作者: etidronate 時(shí)間: 2025-3-27 18:01 作者: LITHE 時(shí)間: 2025-3-28 00:46
https://doi.org/10.1007/978-1-0716-1983-4motor proteins; cytoskeletons; microtubule-binding molecules; microtubule-based materials; microtubule-b作者: 熒光 時(shí)間: 2025-3-28 04:27 作者: 綁架 時(shí)間: 2025-3-28 09:34 作者: Oration 時(shí)間: 2025-3-28 12:44
Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification develop the feature of those devices, it is important to understand the function of microtubule in ., hence, the availability of purified αβ-tubulin is required. Additionally, fluorescently labeled tubulin has become a powerful approach for extensively studying the dynamics of these components. In 作者: Cognizance 時(shí)間: 2025-3-28 15:32 作者: insular 時(shí)間: 2025-3-28 21:17 作者: 溺愛(ài) 時(shí)間: 2025-3-28 23:00
Electro-Modulation of Tubulin Properties and Functions cell division. Microtubules can grow or shrink in response to environmental signals, principally chemical cues. Here, we provide an alternative—physical—strategy to modulate tubulin properties and its self-assembly process. The conformation and electrical properties of tubulin subunits are modulat作者: constellation 時(shí)間: 2025-3-29 06:58
In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Refleles—growth, shrinkage, nucleation, and severing—are regulated by an arsenal of microtubule-associated proteins (MAPs). The activities of many of these MAPs have been reconstituted in vitro using microscope assays. As an alternative to fluorescence microscopy, interference-reflection microscopy (IRM)作者: Interstellar 時(shí)間: 2025-3-29 09:57
Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay Usins. This active transport system can be reconstituted in vitro with the surface-adhered motors transporting the microtubules across a planar surface. In this geometry, the kinesin-microtubule system has been used to study active self-assembly, to power microdevices, and to perform analyte detection. 作者: Ingest 時(shí)間: 2025-3-29 14:26 作者: Osteoporosis 時(shí)間: 2025-3-29 19:20 作者: mastopexy 時(shí)間: 2025-3-29 21:54
Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bi environments. Specifically, by using inherited self-assembly and self-recognition properties of tubulin cytoskeletal protein and by interfacing this protein with lab synthesized carbon nanodots, bio-nano hybrid interfaces were formed. Further manipulation of such biohybrids under the mechanical cyc作者: LINE 時(shí)間: 2025-3-30 01:45
Assembling Microtubule-Based Active Mattertions such as display technology. Active nematics are a new class of liquid crystal materials that are driven out of equilibrium by continuous motion of the constituent anisotropic units. A versatile experimental realization of active nematic liquid crystals is based on rod-like cytoskeletal filamen作者: 人類(lèi)學(xué)家 時(shí)間: 2025-3-30 04:03
Spontaneous Alignment of Microtubules Via Tubulin Polymerization in a Narrow Space Under a Temperatuace and under a temperature gradient are presented for tubulin solutions and tubulin–polymer mixtures. These protocols provide an easy route for hierarchical MT assembly and may extend our current understanding of cytoskeletal protein self-assembly under dissipative conditions.作者: 手術(shù)刀 時(shí)間: 2025-3-30 12:15 作者: 客觀 時(shí)間: 2025-3-30 14:35
Spontaneously Beating Biomimetic Structures perform periodic oscillations. These structures are flagella and cilia. Their beating is driven by the interaction between microtubules and motor proteins and the mechanism regulating this is still a puzzle. One approach to address this issue is the assembling of synthetic minimal systems by using 作者: SEMI 時(shí)間: 2025-3-30 19:31 作者: 放逐某人 時(shí)間: 2025-3-31 00:00
Fabrication of Artificial Muscle from Microtubules, Kinesins, and DNA Origami Nanostructuresrogrammable material offers an opportunity to realize complicated operations through the designing of various nanostructures such as DNA origami. Here we describe the methods to use DNA origami for the self-assembly of the biomolecular motor system, microtubule (MT)-kinesin. A rodlike DNA origami mo
