標題: Titlebook: Microinjection and Transgenesis; Strategies and Proto Angel Cid-Arregui,Alejandro García-Carrancá Book 1998 Springer-Verlag Berlin Heidelbe [打印本頁] 作者: 哄笑 時間: 2025-3-21 18:55
書目名稱Microinjection and Transgenesis影響因子(影響力)
書目名稱Microinjection and Transgenesis影響因子(影響力)學科排名
書目名稱Microinjection and Transgenesis網(wǎng)絡公開度
書目名稱Microinjection and Transgenesis網(wǎng)絡公開度學科排名
書目名稱Microinjection and Transgenesis被引頻次
書目名稱Microinjection and Transgenesis被引頻次學科排名
書目名稱Microinjection and Transgenesis年度引用
書目名稱Microinjection and Transgenesis年度引用學科排名
書目名稱Microinjection and Transgenesis讀者反饋
書目名稱Microinjection and Transgenesis讀者反饋學科排名
作者: Digitalis 時間: 2025-3-21 23:25 作者: 蛙鳴聲 時間: 2025-3-22 02:13
https://doi.org/10.1007/978-3-642-80343-7Vivo; biochemistry; cell; cell culture; chromosome; electron microscopy; gene; gene expression; gene transfe作者: Axillary 時間: 2025-3-22 05:50 作者: 出血 時間: 2025-3-22 10:42
Sperm-Mediated Gene Transfer widely studied, the biological process of fertilization remains still obscure in many aspects, and the molecular steps leading to the formation of the diploid zygote after the interaction of the gametes are not yet entirely clear.作者: 吹牛大王 時間: 2025-3-22 16:31 作者: 后天習得 時間: 2025-3-22 20:31
Supply and Husbandry of Mice for Transgenic ScienceThe use of healthy mice kept under good husbandry conditions is of paramount importance for reliable results in transgenic studies. The health status of mice and their husbandry conditions can have a significant impact on experimental results, and therefore must be considered initially when designing the experiment.作者: 除草劑 時間: 2025-3-22 21:48 作者: coalition 時間: 2025-3-23 01:59
Angel Cid-Arregui,Alejandro García-CarrancáA practical tool presenting the latest techniques developed in renowned laboratories.Easy to follow step-by-step protocols.Background information and full practical details (materials needed, exact re作者: vertebrate 時間: 2025-3-23 08:53
Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysansfer into fertilized eggs and a cloned gene was soon reported to be expressed in mouse somatic tissues (Gordon et al. 1980). Later, recombinant genes were stably introduced into the mouse germ line (Brinster et al. 1981; Wagner et al. 1981). Since then, microinjection has been used routinely to ge作者: 不理會 時間: 2025-3-23 10:46
Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cellsion of a significant number of molecules (Melan and Sluder 1992; Griffiths et al. 1993). Therefore, if the precise localization of a protein is being studied, it will always be necessary to look at the fine structure of the cell, using immunoelectron microscopy techniques. Nevertheless, whenever pos作者: 桉樹 時間: 2025-3-23 14:16 作者: Tremor 時間: 2025-3-23 20:58
Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis?bably been a rare event. One experimental model is provided by a strain of laboratory mice (SWR/J), where efficient provirus acquisition in the female germline has been reported (Jenkins et al. 1985; Panthier et al. 1988).作者: 逃避責任 時間: 2025-3-24 00:36
The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem the mutated allele (see for review Capecchi 1989). ES cell technology has also allowed the use of gene trap strategies to isolate and mutate genes relevant in mouse development (Gossler et al. 1989). Methods for derivation, culture, and genetic modification of ES cells, either by gene targeting or 作者: BLINK 時間: 2025-3-24 03:59
The Generation of Transgenic Mice with Yeast Artificial Chromosomescontaining all the necessary elements for stable maintenance of the artificial chromosome in yeast cells. Telomeric sequences guarantee chromosomal stability. The long vector arm harbors a centromer and an autonomously replicating sequence required for chromosomal segregation and replication, respec作者: 積習已深 時間: 2025-3-24 09:55 作者: ERUPT 時間: 2025-3-24 14:03 作者: Mhc-Molecule 時間: 2025-3-24 15:34 作者: inflate 時間: 2025-3-24 19:43
Gustavo Egea,Teresa Babia,Roser Pagan,Roser Busca,Inmaculada Ayala,Ferran Valderrama,Manuel Reina,Seironment. Thus, monitoring and analysis of these compounds become critical; however, it is very challenging and requires tedious sample preparation and sophisticated analytical instrumentation, such as gas/liquid chromatography coupled with mass spectroscopy (GC/LC-MS). The current chapter focuses o作者: Anterior 時間: 2025-3-25 02:17 作者: mucous-membrane 時間: 2025-3-25 03:44 作者: 影響深遠 時間: 2025-3-25 10:37
Jaime Berumen and performances of the air transport system—airports, airlines, and ATC, disruptive events categorized as external and internal impacting the system’s planned/regular performances, models of the reliability, resilience, robustness, and vulnerability of airports and an air freight/cargo network bas作者: Myofibrils 時間: 2025-3-25 11:42
Robert Hormes,Georg Sczakielice it provides. Indicators linked to each cell can be used to describe system performance and identify areas for further investigation. Additionally, the matrix framework can be used to organize the many government agencies and community institutions across different spatial scales that contribute 作者: 親愛 時間: 2025-3-25 19:11 作者: leniency 時間: 2025-3-25 21:27 作者: insurgent 時間: 2025-3-26 03:52 作者: Meditate 時間: 2025-3-26 06:41
Jane Morrellproach to resilience in extreme situations, as ethical questions run through all disciplines. Building on Paul Ricoeur’s practical philosophy, we describe what an ethical approach to decision-making in extreme situations could look like, as well as its implications for organizations. We show that su作者: 繁重 時間: 2025-3-26 12:13
Minoo Rassoulzadegan,Julien Sage,Valérie Grandjeans actually observed to dramatically change the situation of energy demand and supply in Japan. This manuscript aims to overview the transition of energy supply and demand in Japan after the Fukushima and to discuss the possibility of considering nuclear accident in energy modeling analysis by applyi作者: 蠟燭 時間: 2025-3-26 13:25
Marialuisa Lavitrano,Valentina Lulli,Barbara Maione,Sabina Sperandio,Corrado Spadaforapting them can have much more detrimental effects to individuals, communities, and even societies. We have established the Institute for Resilient Communities in Berkeley, CA in collaboration with Japanese partners to address the needs for better scientific and technological capabilities to assess, 作者: 陪審團 時間: 2025-3-26 17:35
Pierre-Olivier Angrand,Catherine P. Woodroofe,A. Francis Stewartproach to resilience in extreme situations, as ethical questions run through all disciplines. Building on Paul Ricoeur’s practical philosophy, we describe what an ethical approach to decision-making in extreme situations could look like, as well as its implications for organizations. We show that su作者: atopic-rhinitis 時間: 2025-3-26 22:21
Carol Murphy,Luis Martin-Parras,Ulrich Rüthernominal and worst-case operating conditions in modern microprocessor designs grows, the inefficiencies of worst-case design are too large to ignore. Recognizing the inefficiencies in such a design style, researchers have begun to propose architecture-level solutions that address worst-case condition作者: Charitable 時間: 2025-3-27 01:52 作者: bronchiole 時間: 2025-3-27 07:27 作者: 加強防衛(wèi) 時間: 2025-3-27 11:50
tion of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods978-3-540-61895-9978-3-642-80343-7作者: sultry 時間: 2025-3-27 14:39 作者: Hypomania 時間: 2025-3-27 20:16
Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Exesired amount of oligonucleotides inside the cells. Oligonucleotides are injected into cells to inhibit gene expression (Kleuss et al. 1994; Kola and Sumarsono 1995; Moulds et al. 1995) and to analyze the fate of antisense oligonucleotides in cells (Chin et al. 1990; Dagle et al. 1991; Leonetti et al. 1991).作者: 爭吵 時間: 2025-3-28 01:47
Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tis-1 replication was quantified by measurement of the release of infectious HIV-1 particles after amplification of HIV-1 produced in initially microinjected SW480 cells by cocultivated CD4. MT-4 cells (Sczakiel et al. 1990).作者: Outmoded 時間: 2025-3-28 03:22 作者: Jubilation 時間: 2025-3-28 09:31 作者: 招待 時間: 2025-3-28 13:35
Videomicroscopy of Living Microinjected Hippocampal Neurons in Cultureris et al. 1988), and actin dynamics in growth cones (Forscher and Smith 1988). Beside these methods, organelle transport can be also specifically visualized and documented by fluorescent microscopy using specific fluorescent probes (Lee and Chen 1988).作者: 飛鏢 時間: 2025-3-28 16:05 作者: engender 時間: 2025-3-28 19:01
ation and full practical details (materials needed, exact reThe establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ- enced many fields of biology作者: 不規(guī)則的跳動 時間: 2025-3-28 23:17
Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysular recording were developed in the late 1940s. Less than one decade later, similar techniques were applied to perform direct transfer of biological material into cells (see Chambers and Chambers 1961). Initially, microinjection was designed for transplantation of mammalian nuclei (Graessmann 1970)作者: EVEN 時間: 2025-3-29 04:11
Automated Computer-Assisted Microinjection into cultured somatic cellsn (for an overview see Celis 1986), and is now established as one of the most versatile methods of introducing substances into such living cells. Microinjection made it possible to use single cells as objects to study complex cellular processes, structure and function in vivo. A large variety of mol作者: Mortar 時間: 2025-3-29 08:10 作者: SPURN 時間: 2025-3-29 11:58
Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture types of videomicroscopy will be described: videomicroscopy with normal phase contrast, and video enhanced contrast differential interference contrast (VECDIC) microscopy. The first is especially suited to study morphological changes of the cell such as cell movement, retraction, outgrowth, filopod作者: Unsaturated-Fat 時間: 2025-3-29 16:36 作者: 鞭子 時間: 2025-3-29 22:08 作者: Vulnerary 時間: 2025-3-30 00:46 作者: murmur 時間: 2025-3-30 05:12
Cellular Microbiochemistryfer can occur at well-defined stages of the cell cycle, and modifications of culture conditions are possible before, during, and after injection. The number of cells which can be injected per experiment is, however, limited. Therefore, in the past, biochemical analyses of microinjected cells has bee作者: 要求比…更好 時間: 2025-3-30 09:22
A New Strategy for Studying Microtubule Transport and Assembly During Axon Growthrning the mechanisms by which this occurs. The earliest model held that preassembled microtubules are transported from the cell body of the neuron down the growing axon, while subsequent models held that new polymer is added at the distal region of the growing axon via local microtubule assembly. Si作者: 笨拙處理 時間: 2025-3-30 15:04 作者: CRATE 時間: 2025-3-30 19:03
Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis? to methods based on the use of fertilized zygotes, early embryos, or ES cells. These powerful techniques are time consuming and costly. Moreover, they are applicable only to a limited number of species. The search for alternative methods appears therefore as a worthwhile long term prospect. Since t作者: 猛然一拉 時間: 2025-3-31 00:06 作者: 露天歷史劇 時間: 2025-3-31 02:56
A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryoscribed in Hogan et al. (1986). Therefore, here we will only mention steps which deviate from those widely described. We have, over the years, simplified several steps, and consider it worthwhile to document this procedure.作者: GREG 時間: 2025-3-31 06:59
The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stemindefinitely in culture (see for review Robertson 1987). When incorporated into preimplantation stage embryos, ES cells are able to form chimeras. Such embryos develop into adult mice in which ES cells contribute to all tissues, including the germline (reviewed by Robertson 1986). These unique chara作者: 議程 時間: 2025-3-31 11:23 作者: 血統(tǒng) 時間: 2025-3-31 15:02
Angel Cid-Arregui,Carla Santana,Miriam Guido,Nestor Morales-Peza,Maria Victoria Juarez,Roger Wepf,Altural activities, unplanned urbanization, and imprudent management. In addition to these, inevitable climate change may further increase pressure on the water resources. An assessment of the water sources by using the agent-based modeling is necessary in order to understand stress on the available w
