標(biāo)題: Titlebook: Microinjection; Methods and Applicat David J. Carroll Book 2009 Humana Press 2009 Antibodies.Expression.Gene function.Nucleotide.Protein ex [打印本頁] 作者: dejected 時間: 2025-3-21 18:40
書目名稱Microinjection影響因子(影響力)
書目名稱Microinjection影響因子(影響力)學(xué)科排名
書目名稱Microinjection網(wǎng)絡(luò)公開度
書目名稱Microinjection網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Microinjection被引頻次
書目名稱Microinjection被引頻次學(xué)科排名
書目名稱Microinjection年度引用
書目名稱Microinjection年度引用學(xué)科排名
書目名稱Microinjection讀者反饋
書目名稱Microinjection讀者反饋學(xué)科排名
作者: frozen-shoulder 時間: 2025-3-21 23:10 作者: 阻擋 時間: 2025-3-22 04:28 作者: 小官 時間: 2025-3-22 05:05
Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans,intracytoplasmic sperm injection (ICSI) in humans. The following protocols will provide a detailed description of how to perform immunodetection and imaging of human eggs, zygotes, and sperm by fluorescence (confocal and epifluorescence) and electron microscopy.作者: 勉勵 時間: 2025-3-22 11:06
,Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs, The use of luciferase to monitor protein expression after injection of cRNA is a sensitive and effective method that efficiently allows for sets of eggs to be used for PLCζ quantitation, Ca. imaging, and studies of embryo development.作者: BARGE 時間: 2025-3-22 15:42
Analysis of 14-3-3 Family Member Function in , Embryos by Microinjection of Antisense Morpholino Olng 14-3-3 τ exhibited gastrulation and axial patterning defects, but embryos lacking 14-3-3 γ exhibited eye defects without other abnormalities, and embryos lacking 14-3-3 ζ appeared completely normal. These and other results demonstrate the power and specificity of the morpholino antisense oligo microinjection technique.作者: Cardiac-Output 時間: 2025-3-22 19:41
Quantitative Microinjection of Mouse Oocytes and Eggs, an inverted microscope. The techniques are applicable to the oocytes, eggs, and early embryos of most mammalian species. Included are some general instructions on fabrication of transfer pipettes, holding pipettes, beveled injection pipettes, and equipment for quantitative injection.作者: irreparable 時間: 2025-3-22 23:55
1064-3745 bditis elegans, and tissue culture cells.Contains detailed m.As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In .Microinjection: Methods and Applica作者: inundate 時間: 2025-3-23 01:24 作者: 食品室 時間: 2025-3-23 06:09
A Microinjectable Biological System, the , Oocyte, as an Approach to Understanding Signal Transduct of the cell cycle and easily amenable to biochemical, electrophysiological, and cytological studies. We describe how to microinject proteins or peptides in this model and we study, as an example, the Grb2 transduction cascade.作者: voluble 時間: 2025-3-23 11:04
,Bacteriophage φC31 Integrase Mediated Transgenesis in , for Protein Expression at Endogenous Levelsfect sequence fidelity for recognition and recombination between these attachment sites. These imperfect attachment sites, or pseudo-attachment sites, are present in many organisms and have been used to insert transgenes in a variety of species. Here we describe the φC31 integrase approach to make transgenic .. embryos.作者: 上坡 時間: 2025-3-23 16:42
Microinjection of Follicle-Enclosed Mouse Oocytes,e physiology is best studied within an intact follicle. Here we describe methods for quantitative microinjection of follicle-enclosed mouse oocytes, thus allowing the introduction of signaling molecules as well as optical probes into the oocyte within its physiological environment.作者: 吞吞吐吐 時間: 2025-3-23 19:10 作者: 分發(fā) 時間: 2025-3-24 01:34
1064-3745 ctions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls978-1-4939-6090-3978-1-59745-202-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: larder 時間: 2025-3-24 02:40
Jill C. Sible,Brian N. Wrobleo become nodes of the system by themselves. Hence, some pundits prefer to speak of them as ‘nuclear states’ of broader ‘civilizations’ (Huntington, 2002), as parts of ‘a(chǎn) world of regions’ (Katzenstein, 2005) or of broader ‘regional states’ (Ohmae, 1995).作者: anagen 時間: 2025-3-24 06:58 作者: 沒花的是打擾 時間: 2025-3-24 14:15
David J. Carroll,Wei Huaention design measures of different specialties providing a safe barrier against epidemic disasters when the next epidemic strikes. Finally, the specific case application of the epidemic prevention design of the Qianxihui project in Putian, Fujian Province is elaborated in detail.作者: 陶醉 時間: 2025-3-24 16:53
William H. Kinsey a few questions, to be completed before and after an experience in nature of 20/30 min. The investigation requires to be carried out in private gardens, balconies and/or terraces with greenery, public green areas, etc. 225 participants took part to the investigation. Data analysis highlighted the h作者: Fibrin 時間: 2025-3-24 20:24 作者: Ornament 時間: 2025-3-25 01:32
Sebastien Chenuet,Madiha Derouazi,David Hacker,Florian WurmSupply and Open Spaces). The analysis is done at a granular level taking wards which is the smallest administrative unit of a city. The analysis is done using the multiple linear regression method and finding the coefficient of correlation between the dependent variable (infection rate) and independ作者: 用手捏 時間: 2025-3-25 06:25 作者: Scleroderma 時間: 2025-3-25 09:41
Pavan Kadandale,Indrani Chatterjee,Andrew Singsonf the analysis show that the scope and magnitude of metropolitan activity triggered by the pandemic in its early phase have been rather limited and mostly of a reactive nature. At the same time, there is evidence of a growing metropolitan engagement, especially in the framework of the EU Recovery an作者: Conserve 時間: 2025-3-25 14:51 作者: 許可 時間: 2025-3-25 16:48 作者: 反應(yīng) 時間: 2025-3-25 21:48 作者: Integrate 時間: 2025-3-26 00:42
Vanesa Y. Rawe,Héctor Chemess of the value provided to the different stakeholders involved. The chapter provides evidence of the various activities that have led to cost savings in the running of the municipality, efficiency in serving communities, and collaboration between the municipality and its stakeholders. The chapter al作者: Galactogogue 時間: 2025-3-26 04:18 作者: covert 時間: 2025-3-26 08:55
Analysis of Signaling Pathways in Zebrafish Development by Microinjection, cortex from that in the central regions of the cell. The results demonstrate the highly localized nature of calcium signaling in the zebrafish zygote and the implications of this signaling for embryonic development.作者: 減震 時間: 2025-3-26 15:17
Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complemhile the focus of our research is on the organization of the mitotic spindle during cell division and thus the examples utilized are from that system, the approaches described here should be readily applicable to multiple experimental models.作者: Fissure 時間: 2025-3-26 17:50 作者: MELON 時間: 2025-3-26 21:00
Book 2009y successful .Methods in Molecular Biology.? series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls作者: 北極人 時間: 2025-3-27 01:07
Jill C. Sible,Brian N. Wrobleorld War II, geopolitics was associated with Nazi-oriented anti-liberal ideas and, later on, with realist right-wing military circles. Yet, in recent years, the concept of geopolitics has become more widespread as an analytical dimension. A reason for this can be found in the shifts of the internati作者: definition 時間: 2025-3-27 07:17
Karl Swann,Karen Campbell,Yuansong Yu,Christopher Saunders,F. Anthony Laiinuity between the foreign policy of the first years of the Republic of Brazil, particularly from 1902 to 1912, when José Maria da Silva Paranhos Júnior, known as the Baron of Rio Branco, was Brazil’s Minister of External Relations (MRE is its acronym in Portuguese), and the present day. We seek the作者: 使堅(jiān)硬 時間: 2025-3-27 10:54
David J. Carroll,Wei Huagn. The paper compared the number of casualties caused by epidemics and geological and meteorological disasters in history, and analyzed the ways of transmission of novel coronavirus and the building response strategies, concluding the necessity and importance of compiling epidemic prevention standa作者: Indigence 時間: 2025-3-27 16:47 作者: CLEFT 時間: 2025-3-27 19:26 作者: LAPSE 時間: 2025-3-28 01:53
Sebastien Chenuet,Madiha Derouazi,David Hacker,Florian Wurmrought to a grinding halt. It is important therefore for cities to understand the factors which build urban resilience to be in a better state of preparedness. The study aims to study some of the determinants of urban resilience against Covid-19 in the context of 4 large Indian cities across the fir作者: Lamina 時間: 2025-3-28 06:10 作者: 保守 時間: 2025-3-28 06:18
Pavan Kadandale,Indrani Chatterjee,Andrew Singsonave contributed to accelerate and maximise the diffusion of COVID-19. The impact of the pandemic spread outside the core municipalities following fluid functional patterns, in turn calling for actions that do not respond to traditional administrative units but should be tailored to softer functional作者: Bridle 時間: 2025-3-28 12:04 作者: Alveoli 時間: 2025-3-28 15:43
Laurinda A. Jaffe,Rachael P. Norris,Marina Freudzon,William J. Ratzan,Lisa M. Mehlmannca. The communities of Cape Town responded in a timely way to the risks associated with the pandemic to limit its dangerous potential and find solutions to support people in critical situations. These are due to existing critical medical conditions (TB and HIV rates), impossibility to practice physi作者: 我就不公正 時間: 2025-3-28 19:26
Vincenzo Cavalieri,Maria Di Bernardo,Giovanni Spinelliainable cities by development agencies, governments, and academics. Urbanisation brings about challenges in various aspects of living, from transportation and mobility to housing. Factors like uneven economic development, mass consumerism, and excessive utilisation of natural resources, which are co作者: triptans 時間: 2025-3-29 00:37 作者: olfction 時間: 2025-3-29 06:57
Satoshi Kishigami,Teruhiko Wakayama public safety, transportation and poor water supply. Small businesses can take advantage of these opportunities to promote Smart Communities in cities. Although Smart City and Smart Community initiatives in South Africa are in their infancy, they can complement existing Integrated Development Plans作者: Blood-Vessels 時間: 2025-3-29 09:50 作者: 混雜人 時間: 2025-3-29 13:02
,Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs,ein that is expressed in eggs after injection of cRNA is effective in causing Ca. oscillations at very low concentrations. In order to measure the amount and timecourse of protein expression we have tagged PLCζ with firefly luciferase. The expression of the luciferase protein tag in eggs is then mea作者: 持久 時間: 2025-3-29 16:44 作者: 蛛絲 時間: 2025-3-29 21:22
A Microinjectable Biological System, the , Oocyte, as an Approach to Understanding Signal Transductertebrate cell, the . oocyte, is a perfect model system to perform these studies. Oocytes are numerous and synchronous cells, arrested in the G2 phase of the cell cycle and easily amenable to biochemical, electrophysiological, and cytological studies. We describe how to microinject proteins or pepti作者: Contort 時間: 2025-3-30 02:05 作者: 反饋 時間: 2025-3-30 07:01 作者: 背帶 時間: 2025-3-30 09:25
Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complemout the roles of individual proteins in the cell. For many non-genetic model organisms researchers have relied on the use of inhibitory reagents, such as antibodies that can be microinjected into cells. More recently, the advent of RNA-mediated interference (RNAi) has allowed scientists to knockdown作者: 影響深遠(yuǎn) 時間: 2025-3-30 13:49 作者: 先驅(qū) 時間: 2025-3-30 18:21 作者: adhesive 時間: 2025-3-30 23:28 作者: Preserve 時間: 2025-3-31 03:13
Quantitative Microinjection of Mouse Oocytes and Eggs,y large mammalian oocyte or egg is easily manipulated and can be injected with impermeant reagents including a variety of signaling molecules and fluorescent probes. Techniques have been developed to inject picoliter quantities of solution into oocytes and eggs with precision and reliability. The me作者: Rebate 時間: 2025-3-31 06:19
Microinjection of Follicle-Enclosed Mouse Oocytes,and signals from the oocyte regulate the somatic cells. Because isolation of the oocyte from the follicle disrupts these communication pathways, oocyte physiology is best studied within an intact follicle. Here we describe methods for quantitative microinjection of follicle-enclosed mouse oocytes, t
