標(biāo)題: Titlebook: Microinjection; Methods and Protocol Chengyu Liu,Yubin Du Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Gene [打印本頁] 作者: 無限 時(shí)間: 2025-3-21 17:39
書目名稱Microinjection影響因子(影響力)
書目名稱Microinjection影響因子(影響力)學(xué)科排名
書目名稱Microinjection網(wǎng)絡(luò)公開度
書目名稱Microinjection網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Microinjection被引頻次
書目名稱Microinjection被引頻次學(xué)科排名
書目名稱Microinjection年度引用
書目名稱Microinjection年度引用學(xué)科排名
書目名稱Microinjection讀者反饋
書目名稱Microinjection讀者反饋學(xué)科排名
作者: 不能仁慈 時(shí)間: 2025-3-21 21:05 作者: Arctic 時(shí)間: 2025-3-22 02:58 作者: EWER 時(shí)間: 2025-3-22 06:31
Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/633290.jpg作者: 寬敞 時(shí)間: 2025-3-22 12:05
https://doi.org/10.1007/978-1-4939-8831-0Gene editing; Animal model generation; CRISPR; TALEN; Embryo micromanipulations; Genome engineering作者: 違反 時(shí)間: 2025-3-22 15:01 作者: exclusice 時(shí)間: 2025-3-22 17:55 作者: Arthritis 時(shí)間: 2025-3-22 22:29
Xin-an Pu,Anthony P. Young,H. Michael Kubisch. All the chapters are based on untapped original sources, chiefly from the State Archive in Rome, the Vatican Archives, the Rome Municipal Archives, the école Fran?aise Library, the National Library, and the Capitoline Library..978-3-031-41262-2978-3-031-41260-8作者: 蝕刻 時(shí)間: 2025-3-23 02:30 作者: 逢迎白雪 時(shí)間: 2025-3-23 09:18
Yubin Du,Wen Xie,Fan Zhang,Uimook Choi,Chengyu Liu,Colin L. Sweeneyble relation between the concepts of Intellectual Capital, Resilience, Reliability, Sustainability, and Reputation. The research is supported by a data-driven approach, oriented by a path analysis model. This study contributes to the literature on resilience, and it can serve as a support to decisio作者: Recess 時(shí)間: 2025-3-23 11:05
Melissa A. Scott,Yueh-Chiang Huview of the literature and the contribution of experts from several areas for defining the analysis model. Knowledge engineering methods and techniques were used to enable data analysis on integrated operations of companies in the oil and gas sector, thus allowing a systemic view on the conditioning作者: Carcinogenesis 時(shí)間: 2025-3-23 17:23 作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-23 21:57 作者: Intractable 時(shí)間: 2025-3-24 00:34 作者: pulse-pressure 時(shí)間: 2025-3-24 04:33
Toru Takeo,Jorge Sztein,Naomi Nakagata conservation issues arising from extreme natural events and human-induced disturbances in the mediterranean regions. The proceed- ings were issued on the last day of the conference and provided two-page accounts of each of the contributed papers and posters (Dell, B. (ed. ) 1984 Proceedings of the 作者: 保守黨 時(shí)間: 2025-3-24 09:25
Takayuki Sakurai,Akiko Kamiyoshi,Masato Ohtsuka,Channabasavaiah B. Gurumurthy,Masahiro Sato,Takayuki-reorganization. We present a metadata framework implementation which is based on open-source Big Data technologies, describing its architecture and interconnections with external systems, data model, functions, quality metrics, and templates. A test execution of an experimental Airflow Directed Acy作者: Horizon 時(shí)間: 2025-3-24 12:31 作者: SLING 時(shí)間: 2025-3-24 15:21
Xiaoxia Cuimise, there are also concerns that arise from the deployment of robots. Those discussed relate to safety considerations, privacy infringement, cyber-security, and financial aspects, such as the cost of development and maintenance as well as impact on employment.作者: Harness 時(shí)間: 2025-3-24 21:09
Masumi Hirabayashi,Shinichi Hochi-reorganization. We present a metadata framework implementation which is based on open-source Big Data technologies, describing its architecture and interconnections with external systems, data model, functions, quality metrics, and templates. A test execution of an experimental Airflow Directed Acy作者: chapel 時(shí)間: 2025-3-25 02:46
Dongshan Yang,Jie Xu,Y. Eugene Chenprovement of decisions from data and simulations, i.e. the so-called Adaptive Monte Carlo optimization. The SQG methods are applicable in cases when traditional stochastic approximation, gradient or stochastic gradient methods do not work, in particular, to general two-stage problems with implicitly作者: Genteel 時(shí)間: 2025-3-25 03:48 作者: 心胸狹窄 時(shí)間: 2025-3-25 08:51 作者: Allege 時(shí)間: 2025-3-25 14:00 作者: 托運(yùn) 時(shí)間: 2025-3-25 19:33 作者: xanthelasma 時(shí)間: 2025-3-25 22:15
Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique,in pronuclear injection. Moreover, Cytochalasin B that is used to soften zygotes during piezo-driven microinjection has been suggested a role in improving the knockin efficiency, which provides an additional benefit to use this injection method.作者: 吊胃口 時(shí)間: 2025-3-26 01:45 作者: Processes 時(shí)間: 2025-3-26 08:02 作者: 蕁麻 時(shí)間: 2025-3-26 12:05
The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Speathione to the fertilization medium. These modifications greatly enhanced the fertility of frozen-thawed C57BL/6 sperm, resulting in a stable fertilization rate >80% in IVF. Our results indicate that this robust protocol for sperm cryopreservation may improve the archiving and distributing system fo作者: Anticlimax 時(shí)間: 2025-3-26 14:07
Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line,and single hepatocytes (both genome-edited and wild-type) can be isolated for analysis immediately following transient gene editing. Previously, we demonstrated successful isolation of genome-edited single hepatocytes, using mice expressing systemic Cas9 transgene (called “sCAT” mouse) and by tail-v作者: 強(qiáng)壯 時(shí)間: 2025-3-26 18:01 作者: Small-Intestine 時(shí)間: 2025-3-27 00:25 作者: 榨取 時(shí)間: 2025-3-27 03:01
Transgene Recombineering in Bacterial Artificial Chromosomes,ACs due to their size and complexity, thus a specialized field “recombineering” has developed to modify BAC clones through the use of homologous recombination in bacteria with short homology regions. Genetically engineered BACs can then be used in cell culture, mouse, or rat models to study cancer, neurology, and genetics.作者: 貧窮地活 時(shí)間: 2025-3-27 07:11 作者: Heart-Rate 時(shí)間: 2025-3-27 13:05
1064-3745 er half centered on a wide variety of other models.Contains This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impac作者: RALES 時(shí)間: 2025-3-27 15:47 作者: 腐蝕 時(shí)間: 2025-3-27 18:38
Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method,petent to produce offspring. This can be exploited for the generation of genetically modified mice, through the transplantation of genetically modified GS cells. In this section, we describe a method of genome editing-mediated GS cell modification and transplantation.作者: prostate-gland 時(shí)間: 2025-3-28 01:21 作者: 晚來的提名 時(shí)間: 2025-3-28 03:08 作者: Infirm 時(shí)間: 2025-3-28 06:51
Generation of Rabbit Models by Gene Editing Nucleases, now possible to produce gene targeting (i.e., knockout, knockin) rabbits with high success rates. In this chapter, we describe a comprehensive, step-by-step protocol for rabbit genome editing based on gene editing nucleases with specific emphasis of CRISPR/Cas9.作者: 施加 時(shí)間: 2025-3-28 10:24
Book 2019ut also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-edit作者: ARM 時(shí)間: 2025-3-28 16:06
Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation,oinjection has been the technology of choice, electroporation has also emerged and been proven to be effective delivering CRISPR-Cas9 reagents into the mouse zygote. Here, we describe the experimental protocol employing electroporation to deliver CRISPR-Cas9 reagents into mouse embryos and derive gene-edited mutant mice.作者: 拘留 時(shí)間: 2025-3-28 21:13
Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer,ce, including derivation and inactivation of MEF feeder cells, culturing and handling of mouse ES cells, collection and microinjection of blastocysts, and finally implantation of injected blastocysts into the uteri of pseudopregnant surrogate mothers.作者: inhibit 時(shí)間: 2025-3-29 02:28
Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombina as in classic gene targeting. As the result, knockin alleles across many target loci are created in mouse embryonic stem cells and readily transmitted through germline. The knockin alleles created in ES cells can also serve as valuable tools for in vitro stem cell differentiation.作者: habitat 時(shí)間: 2025-3-29 06:57
Wenhao Xu undertook in response to their key challenges. Regular online reflections about their transition into rural teaching were collected through ., a digital tool (see Gibson A, Willis J, Morrison C, Crosswell L, Not losing the plot: creating, collecting and curating qualitative data through a web-based作者: Host142 時(shí)間: 2025-3-29 09:54
Xin-an Pu,Anthony P. Young,H. Michael Kubischational relations, famine, and debt.Based on untapped origin.This book analyses the evolution of the city of Rome, in particular, papal Rome, from the plague of 1656 until 1870 when it became the capital of the Kingdom of Italy. The authors explore papal Rome as a resilient city that had to cope wit作者: 憤怒事實(shí) 時(shí)間: 2025-3-29 14:40 作者: flammable 時(shí)間: 2025-3-29 16:03
Yubin Du,Wen Xie,Fan Zhang,Uimook Choi,Chengyu Liu,Colin L. Sweeneyor challenges. In recent years, with the emergence of sustainability issues, particularly the climate issue, resilience has been associated with countries’ sustainability. Furthermore, many resilience attributes seem to be founded and reinforced through intellectual capital components. Nowadays, res作者: Gingivitis 時(shí)間: 2025-3-29 23:48 作者: 宮殿般 時(shí)間: 2025-3-30 00:41 作者: creditor 時(shí)間: 2025-3-30 06:07
Ye Liu,Yubin Du,Wen Xie,Fan Zhang,Douglas Forrest,Chengyu Liurception, particularly in terms of timeframe between parties in land use conflicts..A brief review is made of conflicts reported from Mediterranean climate ecosystems which indicate that there is no universal pattern and hence no universal prescription for resolving conflicts in land use. This appea作者: 分期付款 時(shí)間: 2025-3-30 08:16 作者: 水土 時(shí)間: 2025-3-30 13:31 作者: jovial 時(shí)間: 2025-3-30 17:18
Toru Takeo,Jorge Sztein,Naomi Nakagata Western Australia during August 1984. It thus follows three previous meetings, Chile (1971), California (1977) and South Africa (1980). There has been no formal international body to organize these meetings, merely a continuity of purpose provided by the common interests of the scientists concerned作者: expansive 時(shí)間: 2025-3-30 21:01 作者: 運(yùn)動性 時(shí)間: 2025-3-31 04:34 作者: GEST 時(shí)間: 2025-3-31 05:17 作者: 截?cái)?nbsp; 時(shí)間: 2025-3-31 11:16 作者: fibula 時(shí)間: 2025-3-31 17:02 作者: 多余 時(shí)間: 2025-3-31 18:43 作者: 歌唱隊(duì) 時(shí)間: 2025-4-1 00:16 作者: Barter 時(shí)間: 2025-4-1 04:04 作者: 有效 時(shí)間: 2025-4-1 08:54 作者: 混雜人 時(shí)間: 2025-4-1 11:38
Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method,ves by self-renewing proliferation while retaining the ability to differentiate into sperm. Thus, when transplanted into the seminiferous tubules of a host mouse testis, they settle in the basal compartment of the tubules and establish spermatogenenic colonies. The sperm produced in the host are com作者: 胰臟 時(shí)間: 2025-4-1 16:52 作者: 憂傷 時(shí)間: 2025-4-1 20:14 作者: Minuet 時(shí)間: 2025-4-2 00:40 作者: 無底 時(shí)間: 2025-4-2 06:21
