作者: 不可思議 時(shí)間: 2025-3-22 00:03
Using Electrophoretic Immunoassay to Monitor Hormone Secretiontrophoresis assay that allows cell culture and hormone monitoring. An online gradient generator can control cell culture condition precisely. This system has been applied for Pancreas islets’ glucose sensitivity studies.作者: 欺騙手段 時(shí)間: 2025-3-22 03:03
On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnosticsnd methodology for performing rapid immunoassays on magnetic particles in continuous flow via their deflection through multiple laminar flow streams of reagents and washing solutions. In particular, we focus on the use of the microfluidic platform for a C-reactive protein (CRP) sandwich immunoassay in less than 60 s.作者: moratorium 時(shí)間: 2025-3-22 04:39
Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic ChipCTCs would be enriched from very small samples, with minimal handling, high recovery, and no requirement for the expression of specific surface markers. Here, we describe negative enrichment as the preferred approach for cancer cell isolation using a microfluidic platform.作者: lanugo 時(shí)間: 2025-3-22 10:32
Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reactionll array patterned in the Teflon-AF. surface of the grounding plate of the chip. Finally, the potential of the device for its application to single-molecule detection is demonstrated by the ultrasensitive detection of the biotinylated enzyme β-Galactosidase captured on streptavidin-coated particles in the described platform.作者: 精致 時(shí)間: 2025-3-22 13:59
Microfluidic DNA Stretching Device for Single-Molecule Diagnosticsrooves in a densely-arranged molecular patterning apparatus in a microfluidic channel. By syringing through the microchannel, over 1500 DNA molecules can be arrayed simultaneously in the microgrooves. It will therefore serve as a template preparation for DNA molecular diagnosis by high-resolution imaging.作者: 討人喜歡 時(shí)間: 2025-3-22 18:48 作者: Legion 時(shí)間: 2025-3-22 21:39 作者: 殘酷的地方 時(shí)間: 2025-3-23 02:17
Droplet Microfluidic and Magnetic Particles Platform for Cancer Typingown to the single cell level..We report on a droplet microfluidic platform based on the manipulation of magnetic particles that allows the clinical analysis of tumor tissues. In particular, it allows the extraction of mRNA from the total-RNA sample, Reverse Transcription, and cDNA amplification, all in droplets.作者: RENIN 時(shí)間: 2025-3-23 08:53
Book 2017t, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..作者: 生銹 時(shí)間: 2025-3-23 12:22
Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealinganing, and drying steps. Then, integration of reagents (if any) can be done, followed by lamination of a sealing cover. Breaking by hand the partially diced wafer yields individual chips ready for use.作者: 陪審團(tuán)每個(gè)人 時(shí)間: 2025-3-23 15:14
Book 2017hip Diagnostics: Methods and Protocols is .divided into four sections describing the business aspect.s. of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis作者: 積云 時(shí)間: 2025-3-23 19:33
1064-3745 ation advice from the experts.This volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. .Microchip Diagnostics: Methods and Protocols is .divided into four sections describing the business aspect.s. of the microfluid作者: engrossed 時(shí)間: 2025-3-24 01:54 作者: Substance-Abuse 時(shí)間: 2025-3-24 06:04
Droplet-Based Microfluidics Digital PCR for the Detection of KRAS MutationsWe demonstrate an accurate and sensitive quantification of mutated . oncogene in genomic DNA, using droplet-based microfluidics and digital PCR.作者: Kinetic 時(shí)間: 2025-3-24 07:46 作者: amenity 時(shí)間: 2025-3-24 12:50 作者: TAIN 時(shí)間: 2025-3-24 15:21 作者: 特征 時(shí)間: 2025-3-24 23:02
https://doi.org/10.1007/978-1-4939-6734-6microfluidic approaches; molecular biomarkers; cellular biomarkers; cell analysis; microfluidics; protei作者: 瑣碎 時(shí)間: 2025-3-24 23:10
978-1-4939-8286-8Springer Science+Business Media LLC 2017作者: 小畫像 時(shí)間: 2025-3-25 05:07
Microchip Diagnostics978-1-4939-6734-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Perceive 時(shí)間: 2025-3-25 11:23 作者: 嬉耍 時(shí)間: 2025-3-25 12:30
Yuksel Temiz,Robert D. Lovchik,Emmanuel Delamarcheoodnature research. Educational studies in an Anthropocene era have often positioned “childhoodnature” research in the more familiar humanist perspective according prominence to the centered subject, representation, and interpretation. This approach to research has been the source of criticism for n作者: NORM 時(shí)間: 2025-3-25 17:09
Toshihiro Kasama,Noritada Kaji,Manabu Tokeshi,Yoshinobu Babanally designed research, invoking epistemological and ontological shifts. Both epistemological and ontological shifts have brought to attention the complexity and plurality of children and childhoods and highlighted its epistemologically unstable structures. In this chapter, we follow this trend to 作者: Jejune 時(shí)間: 2025-3-25 23:43
Shusheng Lu,Robert T Kennedyn a journey from how the UK Research Excellence Framework assesses impact to real examples of outstanding research impact case studies.? Along the way, Prof. Hugh McKenna describes and explains the case for research impact, the challenges, the link between research impact and evidence informed pract作者: hedonic 時(shí)間: 2025-3-26 00:54 作者: exclusice 時(shí)間: 2025-3-26 05:39 作者: moratorium 時(shí)間: 2025-3-26 09:05 作者: 笨拙的我 時(shí)間: 2025-3-26 13:29
Davide Ferraro,Jér?me Champ,Bruno Teste,M. Serra,Laurent Malaquin,Stéphanie Descroix,Patricia de Creeum settings, and will be of considerable interest to the muMuseums are institutions of both education and learning in service of society, that is, they are sites where educational experiences are designed and facilitated, and also places where visitors learn in broad and diverse ways. As such, the 作者: 群島 時(shí)間: 2025-3-26 18:19
Feng Sheneum settings, and will be of considerable interest to the muMuseums are institutions of both education and learning in service of society, that is, they are sites where educational experiences are designed and facilitated, and also places where visitors learn in broad and diverse ways. As such, the 作者: 大包裹 時(shí)間: 2025-3-26 22:27
Deniz Pekin,Valerie Taly PhDeum settings, and will be of considerable interest to the muMuseums are institutions of both education and learning in service of society, that is, they are sites where educational experiences are designed and facilitated, and also places where visitors learn in broad and diverse ways. As such, the 作者: FILTH 時(shí)間: 2025-3-27 04:43 作者: 好色 時(shí)間: 2025-3-27 08:15 作者: V切開 時(shí)間: 2025-3-27 09:36 作者: Agnosia 時(shí)間: 2025-3-27 16:08
Séverine Le Gac PhDfacilitated, and also places where visitors learn in broad and diverse ways. As such, the role of public education in museums today is highly important, if not at the centre of museum activity. As museums contemplate the growing significance of their educational roles and mandate within a changing s作者: Seminar 時(shí)間: 2025-3-27 21:33 作者: forebear 時(shí)間: 2025-3-27 23:43 作者: STING 時(shí)間: 2025-3-28 04:41 作者: invulnerable 時(shí)間: 2025-3-28 08:27 作者: gusher 時(shí)間: 2025-3-28 13:50 作者: Coronation 時(shí)間: 2025-3-28 17:20 作者: 飲料 時(shí)間: 2025-3-28 22:20
Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reactionil how to build up an electrowetting-on-dielectric-based digital microfluidic chip with unique advantages for performing single-molecule detection. We illustrate how superparamagnetic particles can be printed with very high loading efficiency (over 98 %) and single-particle resolution in the microwe作者: 倔強(qiáng)不能 時(shí)間: 2025-3-28 23:58
Microfluidic DNA Stretching Device for Single-Molecule Diagnosticsodification of the DNA or surface modification of the substrate. To detect a signal variation caused by sequence-specific dye binding or partial melting, it is crucial that the DNA molecules are arrayed in a parallel direction inside the narrow microscopic field. The method uses zigzag-shaped microg作者: 原諒 時(shí)間: 2025-3-29 05:07 作者: 匯總 時(shí)間: 2025-3-29 11:10
SlipChip Device for Digital Nucleic Acid Amplificationpartitions and performing parallel nucleic acid amplification, such as polymerase chain reaction (PCR) or isothermal amplification reactions. With the counts of positive wells, total number of wells, and volumes of wells, the concentration of the target nucleic acid in the sample can be quantified. 作者: Emg827 時(shí)間: 2025-3-29 12:23
Multiplex Detection of , Mutations Using Passive Droplet Fusionnomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the . oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly showing that the technique is quantitat作者: Cleave 時(shí)間: 2025-3-29 16:30
Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chipspecifically as a companion diagnostic for new anticancer drugs, and for research into the mechanisms of disease progression and metastases. Ideally, CTCs would be enriched from very small samples, with minimal handling, high recovery, and no requirement for the expression of specific surface marker作者: 確定無疑 時(shí)間: 2025-3-29 23:39
Microfluidic-Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection shock, and multi organ failure syndrome. Blood culturing is currently the gold standard for the identification of microorganisms and, although it has been automated over the decade, the process still requires 24–72 h to complete. This long turnaround time, especially for the identification of antim作者: fledged 時(shí)間: 2025-3-30 01:03 作者: 滔滔不絕的人 時(shí)間: 2025-3-30 06:08 作者: Ornithologist 時(shí)間: 2025-3-30 08:54
Microfluidic Platform for Parallel Single Cell Analysis for Diagnostic Applicationsr personalized medicine, not only because of the heterogeneity of the cell sample, but also due to the availability of a reduced amount of cells, or even unique cells. This chapter presents a versatile platform meant for the parallel analysis of individual cells, with a particular focus on diagnosti作者: 假裝是你 時(shí)間: 2025-3-30 15:37 作者: 桉樹 時(shí)間: 2025-3-30 19:54
