作者: Blood-Clot 時(shí)間: 2025-3-21 22:10 作者: evince 時(shí)間: 2025-3-22 00:28 作者: 停止償付 時(shí)間: 2025-3-22 04:34
Antisense RNA Elements for Downregulating Expression,yl-carrier-protein] transacylase as an example to describe the asRNA design with reliable and controllable interference efficiency. Real-time PCR and fluorescence assay methods are introduced to detect the interference efficiency at RNA level and protein level, respectively.作者: 爆米花 時(shí)間: 2025-3-22 09:28
Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering, recombination. A strong selective pressure for gene duplication can be applied by increasing antibiotic concentration, and in a week’s time one can create a set of strains with a wide range of cassette copy numbers (upward of 20×), which can be made stable by deletion of .. Herein, we describe a generalized workflow for this methodology.作者: anthropologist 時(shí)間: 2025-3-22 16:15
Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant , detailed including methods to test promoter strength using the green fluorescent protein reporter. Furthermore, a method for markerless transformation of chromosomal DNA is presented. Sufficient details are provided to enable application by the novice investigator.作者: 憲法沒有 時(shí)間: 2025-3-22 19:33
1064-3745 ation advice from the expertsThis volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part T作者: CLASP 時(shí)間: 2025-3-22 23:46
Assembly of Complex Pathways Using Type IIs Restriction Enzymes,red for the construction strategy. Basic parts that are not yet available need to be made. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.作者: Defraud 時(shí)間: 2025-3-23 03:06
Checks and Balances with Use of the Keio Collection for Phenotype Testing,es that can be employed to ensure robust phenotype assessment of mutations in the library. These include procedures for thorough confirmation of gene deletions by PCR, phage transduction of mutated loci to new host strains, and strategies for genetic complementation.作者: 庇護(hù) 時(shí)間: 2025-3-23 05:53 作者: GEM 時(shí)間: 2025-3-23 12:32
Delta Integration CRISPR-Cas (Di-CRISPR) in , pathways into the . genome. Detailed protocols are provided on the entire workflow which includes pDi-CRISPR plasmid and donor DNA construction, Di-CRISPR-mediated integration and analysis of integration efficiencies and copy numbers through flow cytometry and quantitative polymerase chain reaction (qPCR).作者: 讓步 時(shí)間: 2025-3-23 14:33
Combinatorial Assembly of Multigene Pathways by Combining Single-Strand Assembly with Golden Gate Alucidated which enables one to modulate the expression via promoter and/or RBS randomization. Moreover, a new combinatorial multigene pathway assembly scheme based on single-strand assembly (SSA) methods and Golden Gate Assembly is introduced, exploiting the strengths of both assembly techniques.作者: GRACE 時(shí)間: 2025-3-23 19:04 作者: insecticide 時(shí)間: 2025-3-23 23:14
Bacterial Expression of Membrane-Associated Cytochrome P450s and Their Activity Assay in Nanodiscs,purify eukaryotic membrane bound cytochrome P450s. We also describe the measurement of the activity of the cytochrome P450 expressed by taking the example of cytochrome P450 2J2, the primary P450 expressed in the human heart and CYP725A4, the primary cytochrome P450 expressed in the first step of ta作者: Palliation 時(shí)間: 2025-3-24 04:13
Genetic Tools for Streamlined and Accelerated Pathway Engineering in ,imicrobial pigment violacein was rapidly assembled in 1?week to demonstrate the simplicity and effectiveness of integrating pathway-balancing strategies with our YaliBrick vectors. In the end, we incorporated CRISPR-Cas9 into our YaliBrick vectors and achieved indel mutation and frameshift gene dele作者: Highbrow 時(shí)間: 2025-3-24 10:15 作者: 幼兒 時(shí)間: 2025-3-24 11:00
A Protocol for the Construction and Curation of Genome-Scale Integrated Metabolic and Regulatory Netomics and phenomics data. GEMINI represents the first attempt at applying well-established curation tools that exist for metabolic networks to be applied for curating regulatory networks. The integrated network models generated by these approaches enable the mechanistic integration of diverse biolo作者: 使人入神 時(shí)間: 2025-3-24 14:59 作者: Leisureliness 時(shí)間: 2025-3-24 21:33 作者: evasive 時(shí)間: 2025-3-25 00:44 作者: lavish 時(shí)間: 2025-3-25 05:13 作者: 絕食 時(shí)間: 2025-3-25 07:52 作者: Mitigate 時(shí)間: 2025-3-25 14:41 作者: Fecundity 時(shí)間: 2025-3-25 16:35 作者: 言行自由 時(shí)間: 2025-3-25 21:01
1064-3745 terials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting edge and authoritative, .Microbial 978-1-4939-9142-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Pessary 時(shí)間: 2025-3-26 00:24
James Jeffryes,Jonathan Strutz,Chris Henry,Keith E. J. Tyo作者: Tracheotomy 時(shí)間: 2025-3-26 05:39 作者: 小木槌 時(shí)間: 2025-3-26 10:47 作者: Finasteride 時(shí)間: 2025-3-26 14:18
Jacob Sebesta,Allison Werner,Christie Ann Marie Peebles作者: charisma 時(shí)間: 2025-3-26 19:24
Pathway and Gene Discovery from Natural Hosts and Organisms,velopments in computational identification of biosynthetic gene clusters, molecular biology, synthetic biology, and analytical tools, this has revealed genes for enzymes with optimal and targeted function, as well as a rich pool of uncharacterized metabolic pathways. This chapter discusses different作者: 發(fā)怨言 時(shí)間: 2025-3-27 00:24
Metabolic In Silico Network Expansions to Predict and Exploit Enzyme Promiscuity,pacities often go unnoticed in the enzyme’s native context but can become apparent in engineered metabolism when the enzyme is exposed to novel substrates or high concentrations of pathway intermediates. This chapter describes how to use metabolic in silico network expansion (MINE) databases to pred作者: 退出可食用 時(shí)間: 2025-3-27 03:00 作者: Interlocking 時(shí)間: 2025-3-27 07:10
Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering,, flanking an antibiotic selection marker and a gene of interest with identical regions of homology permits gene duplication via . mediated homologous recombination. A strong selective pressure for gene duplication can be applied by increasing antibiotic concentration, and in a week’s time one can c作者: Mutter 時(shí)間: 2025-3-27 12:04
Bacterial Expression of Membrane-Associated Cytochrome P450s and Their Activity Assay in Nanodiscs,nction studies. Recently, there have been several instances of expressing eukaryotic membrane bound CYPs in bacteria after making various modifications to both the N-terminus membrane binding domains of the protein and to noncontiguous F-G membrane binding loop that is also implicated in substrate b作者: gruelling 時(shí)間: 2025-3-27 17:13 作者: TOXIN 時(shí)間: 2025-3-27 18:02 作者: 附錄 時(shí)間: 2025-3-27 21:57 作者: 壓艙物 時(shí)間: 2025-3-28 04:05
Checks and Balances with Use of the Keio Collection for Phenotype Testing,ce cassette in place of a single nonessential gene. This mutant library has proven to be incredibly useful in the fields of bacteriology, chemical genomics, biotechnology, and systems biology, which is evidenced by the greater than 3800 citations that the article describing its construction has garn作者: Nonthreatening 時(shí)間: 2025-3-28 10:15 作者: harbinger 時(shí)間: 2025-3-28 14:10
Genetic Tools for Streamlined and Accelerated Pathway Engineering in ,hough it has a long history of industrial applications, the number of genetic tools available to effectively and efficiently engineer . still falls behind the vast number of tools available for common organisms such as . and .. In this protocol, we have developed a complete and versatile genetic too作者: 迅速成長 時(shí)間: 2025-3-28 17:55
,Gas Chromatography–Mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization it has great potential for applications in metabolic engineering. While a number of the analytical techniques used widely in metabolomics are familiar to metabolic engineers performing post hoc analyses of product titers, the requirements for accurately capturing metabolism at a systems scale rathe作者: delta-waves 時(shí)間: 2025-3-28 19:36
Targeted Mass Spectrometry-Based Proteomics Tools for Strain Optimization,ity. The effectiveness of strain optimization relies on methodologies used to aid optimization of native or novel pathways within cells. Many different factors, including mRNA abundance, protein abundance, and enzyme activity/stability, will contribute to the strain performance, which is not often e作者: abstemious 時(shí)間: 2025-3-28 23:09
A Protocol for the Construction and Curation of Genome-Scale Integrated Metabolic and Regulatory Ne many organisms. Yet the complexity of metabolic regulation often limits the accuracy of these models. Integrative modeling approaches have recently been developed that combine metabolic and regulatory networks, thereby expanding the capabilities and accuracy of genome-scale modeling. This chapter p作者: 逢迎白雪 時(shí)間: 2025-3-29 06:13
13C-Fingerprinting and Metabolic Flux Analysis of Bacterial Metabolisms,obial hosts. This mapping of the intracellular network within microbes has proven to be essential for understanding the cell physiology. The approach is also a key to identifying central metabolic nodes, probing the rigidity of a metabolic network, revealing cofactor balances, and delineating hidden作者: 使混合 時(shí)間: 2025-3-29 08:29 作者: calumniate 時(shí)間: 2025-3-29 14:39
McKenzie L. Smith,April M. Miguez,Mark P. Styczynskinen über reelle beschr?nkte Intervalle folgt die Besprechung von Eigenschaften und Auswertungsm?glichkeiten. Inhalt des zweiten Teils dieses Paragraphen ist dann die Integration über allgemeine Wege der komplexen Zahlenebene. Die Definition reeller Wegintegrale schlie?t den ersten Paragraphen ab. Ze作者: 反饋 時(shí)間: 2025-3-29 16:05
Hsien-Chung Tseng,Christine Nicole S. Santosonalen Funktionen müssen wir zun?chst die Nullstellenmenge des Nennerpolynoms aus der Definitionsmenge ausgrenzen. Bei der Untersuchung der Umkehreigenschaften der Exponentialfunktion im zweiten Paragraphen sto?en wir zum ersten Mal auf das Problem der Mehrdeutigkeit. Anstatt auf einer geeigneten Ri作者: 華而不實(shí) 時(shí)間: 2025-3-29 23:39
Sriram Chandrasekaranationalen Funktionen müssen wir zun?chst die Nullstellenmenge des Nennerpolynoms aus der Definitionsmenge ausgrenzen. Bei der Untersuchung der Umkehreigenschaften der Exponentialfunktion im zweiten Paragraphen sto?en wir zum ersten Mal auf das Problem der Mehrdeutigkeit. Anstatt auf einer geeigneten作者: Allege 時(shí)間: 2025-3-30 03:17
Whitney Hollinshead,Lian He,Yinjie J. Tangeantwortet. Mit der umgekehrten Fragestellung, n?mlich der Entwickelbarkeit holomorpher Funktionen durch diese beiden Reihentypen, besch?ftigen sich nun die ersten beiden Paragraphen dieses sechsten Kapitels. Auf jeder Kreisscheibe, die im Holomorphiebereich liegt, kann eine holomorphe Funktion in e作者: GLOOM 時(shí)間: 2025-3-30 06:31
Manisha Wadhwa,Anand K. Bachhawattrie“ sowie der strukturierten curricularen Fortbildung ?Geriatrische Grundversorgung“ und wendet sich an alle ?rzte, die diese Zusatzqualifikationen anstreben. ?Die 2. Auflage erscheint bereits nach kurzer Zeit komplett aktualisiert und korrigiert.?.Das Werk vermittelt praxisnah und auf den Punkt g作者: Panacea 時(shí)間: 2025-3-30 09:19 作者: 相一致 時(shí)間: 2025-3-30 13:29
https://doi.org/10.1007/978-1-4939-9142-6biochemical pathways; promoter libraries; ribosome binding site design; CLIVA; genome editing作者: hermitage 時(shí)間: 2025-3-30 20:28 作者: Impugn 時(shí)間: 2025-3-30 22:32 作者: 僵硬 時(shí)間: 2025-3-31 00:54
Microbial Metabolic Engineering978-1-4939-9142-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 朦朧 時(shí)間: 2025-3-31 06:51
Book 2024deficiencies of the EU rule of law toolbox ten years after the first ad hoc measures were adopted, also offering some recommendations on how to turn dissensus into constructive mechanisms to improve the management of the rule of law in the EU..作者: dialect 時(shí)間: 2025-3-31 09:24 作者: 首創(chuàng)精神 時(shí)間: 2025-3-31 17:06
