作者: GULLY 時(shí)間: 2025-3-21 21:00 作者: 津貼 時(shí)間: 2025-3-22 03:33
Min Jeong KyeIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts作者: ENNUI 時(shí)間: 2025-3-22 08:06
978-1-4939-8407-7Springer Science+Business Media LLC 2017作者: Jejune 時(shí)間: 2025-3-22 11:09
MicroRNA Technologies978-1-4939-7175-6Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: 使混合 時(shí)間: 2025-3-22 16:15
0893-2336 oals. . . Cutting-edge and practical, .MicroRNA Technologies. is a valuable resource for students and researchers who are interested about microRNAs in nervous systems..978-1-4939-8407-7978-1-4939-7175-6Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: Digest 時(shí)間: 2025-3-22 17:04 作者: 斥責(zé) 時(shí)間: 2025-3-22 23:44 作者: 野蠻 時(shí)間: 2025-3-23 01:42 作者: sparse 時(shí)間: 2025-3-23 06:43
Anna Emde,Natalia Rivkin,Mark A. Behlke,Eran Hornstein作者: 身心疲憊 時(shí)間: 2025-3-23 10:15 作者: 蜿蜒而流 時(shí)間: 2025-3-23 16:15 作者: Proclaim 時(shí)間: 2025-3-23 18:39
Functional Analysis of Cortical Neuron Migration Using miRNA Silencing,loss of function approaches that support a role for miR-22 and miR-124 in radial migration and multipolar–bipolar transition of cortical projection neurons. These distinct loss of function methods provide complementary information and results indicate that, depending of the scientific question, one 作者: 消息靈通 時(shí)間: 2025-3-23 22:17
Experimental Methods for Functional Studies of microRNAs in Animal Models of Psychiatric Disorders,c experimental approaches we apply to study the role of miRNAs in this model and demonstrate that miR-33 regulates the susceptibility to state-dependent learning induced by inhibitory neurotransmission.作者: 離開 時(shí)間: 2025-3-24 05:06 作者: CERE 時(shí)間: 2025-3-24 07:22
Pierre-Paul Prévot,Marie-Laure Volvert,Alexander Deiters,Laurent Nguyenighlighted. The proposed approach along with conducted measurements to evaluate it are described in the remaining chapters of the book.978-3-662-59792-7978-3-662-59793-4Series ISSN 2522-8579 Series E-ISSN 2522-8587 作者: heart-murmur 時(shí)間: 2025-3-24 12:07 作者: NUDGE 時(shí)間: 2025-3-24 17:41
Erratum to: Experimental Methods for Functional Studies of microRNAs in Animal Models of Psychiatri作者: obscurity 時(shí)間: 2025-3-24 20:33 作者: 舊式步槍 時(shí)間: 2025-3-25 03:08
Lara Stevanato,Caroline Hicks,Lavaniya Thanabalasundaram,John D. Sindenen jedoch einen unterschiedlichen Zeitrahmen voraus (episodenbezogene bzw. aktuelle Symptomatik) und orientieren sich an unterschiedlichen Validierungskriterien. Die Pr?diktion des Therapieansprechens und des Langzeitverlaufs sowie das Ausma? der famili?ren Belastung sind die allgemein akzeptierten 作者: PRISE 時(shí)間: 2025-3-25 06:27 作者: 致命 時(shí)間: 2025-3-25 11:20
Min Jeong Kyeconstructed to test the viability of these techniques by executing programs containing recovery blocks on an emulator for the proposed architecture. Experience in running this system are recounted with respect to the execution of programs based on erroneous algorithms and also with respect to errors作者: 切割 時(shí)間: 2025-3-25 13:48
Nicole H. Wilson,Esther T. Stoeckli issues and techniques, with an emphasis on replication applied in Web Services settings...With its well-focused approach and clarity of presentation, this book is an excellent resource for both advanced students and practitioners in computer science, computer networks and distributed systems. Anyon作者: Biofeedback 時(shí)間: 2025-3-25 16:33
Vladimir Jovasevic,Jelena Radulovic issues and techniques, with an emphasis on replication applied in Web Services settings...With its well-focused approach and clarity of presentation, this book is an excellent resource for both advanced students and practitioners in computer science, computer networks and distributed systems. Anyon作者: 安撫 時(shí)間: 2025-3-25 20:02 作者: 煩躁的女人 時(shí)間: 2025-3-26 01:31 作者: Aqueous-Humor 時(shí)間: 2025-3-26 06:30
Lara Stevanato,Caroline Hicks,Lavaniya Thanabalasundaram,John D. Sindennnten ?tiologie und Pathophysiologie depressiver Syndrome konfrontiert. Daher müssen Beurteilungssysteme zur Diagnosestellung und Schweregradmessung weitgehend auf psychopathologische Merkmale gestützt werden; die Validierung dieser Beurteilungssysteme mu? sich auf indirekte Indikatoren (Validierung作者: enumaerate 時(shí)間: 2025-3-26 10:52 作者: 新星 時(shí)間: 2025-3-26 14:15 作者: medium 時(shí)間: 2025-3-26 18:59
Ilya A. Vinnikov,Andrii Domanskyi,Witold Konopka-based insights into the reliability of today’s InternetThis thesis describes the research done regarding communication reliability when using the Internet to realize the communications for cyber-physical systems (CPSs). In a nutshell, the book first presents the obtained results from real-world mea作者: AMPLE 時(shí)間: 2025-3-27 00:21
Min Jeong Kyeesidual errors. Recovery blocks allow a software designer to include tests on the acceptability of the various phases of a system’s operation, and to specify alternative actions should the acceptance tests fail. This approach relies on certain architectural features, ideally implemented in hardware,作者: 序曲 時(shí)間: 2025-3-27 01:39 作者: 使無(wú)效 時(shí)間: 2025-3-27 07:36 作者: disciplined 時(shí)間: 2025-3-27 12:36 作者: Felicitous 時(shí)間: 2025-3-27 14:38
Vladimir Jovasevic,Jelena Radulovicse involved in designing and deploying the next generation of mission-critical applications and Web Services. .Reliable Distributed Systems .reviews and describes the key concepts, principles and applications of modern distributed computing systems and architectures. This self-contained book consist作者: MOTTO 時(shí)間: 2025-3-27 21:49 作者: Influx 時(shí)間: 2025-3-27 23:37
Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord, miRNAs play numerous roles in neuro-development and are crucial for the proper homeostatic function of neurons in adults. The detection of miRNAs in specific cell types sheds light on differential regulation of their expression and on the potential functional roles of miRNAs in the brain. In this c作者: Dawdle 時(shí)間: 2025-3-28 06:03
Protocol for High-Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor NeurRNA (miRNA) impact on primary motor neuron. High-content screening (HCS) platforms facilitate superior precision in research and are scalable to study in parallel multiple genetic, molecular, or cellular conditions. miRNAs are critical for neuronal function and for brain integrity and are considered作者: Increment 時(shí)間: 2025-3-28 09:57
Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-radioactive Fluorescence Metds and improves the ability to quantitatively measure the catalytic efficacy of the Dicer complex under various cellular conditions via non-radioactive, emitted fluorescence signal readout. Because radioactive labeling is substituted by measuring fluorescent signal the method is easy to handle. Addi作者: Adj異類的 時(shí)間: 2025-3-28 10:32
Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treaerm memory and learning disabilities in animal models. The underlying mechanisms of anesthetic-induced neurotoxicity are complex and not well understood. In addition, there is no direct clinical evidence of the detrimental effects of anesthetics in human fetuses, infants, or children. Development of作者: ectropion 時(shí)間: 2025-3-28 16:07
MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Trmination (Ambros, Nature 431(7006):350–355, 2004). Here, we evaluated appropriate 3 three dimensional (3D) substrates to differentiate human neural stem cells (hNSCs). We identified and quantified hNSC miRNA contents by PCR array. By using computational algorithms we predicted miRNA target mRNA whi作者: fiction 時(shí)間: 2025-3-28 21:00 作者: 抑制 時(shí)間: 2025-3-28 23:38
Functional Analysis of Cortical Neuron Migration Using miRNA Silencing,ors of gene expression. The validation of miRNA-target interactions in animal model systems is not trivial, especially in the developing cerebral cortex. Induction of miRNAs loss-of-function is the ideal way to study their physiological role in vivo. Although it has been accepted that the dramatic b作者: 欺騙手段 時(shí)間: 2025-3-29 06:05 作者: 連接 時(shí)間: 2025-3-29 08:36 作者: 流動(dòng)性 時(shí)間: 2025-3-29 15:09 作者: hysterectomy 時(shí)間: 2025-3-29 15:41
Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs,egulated in a spatial and temporal manner. Recent studies have uncovered important roles of small noncoding RNAs in injury responses of axons during regeneration, particularly via translational regulation of axonally localized mRNAs. Several different approaches have been primarily focused on in vit作者: Mawkish 時(shí)間: 2025-3-29 22:19 作者: Commission 時(shí)間: 2025-3-30 00:16 作者: 拾落穗 時(shí)間: 2025-3-30 05:44 作者: EWER 時(shí)間: 2025-3-30 09:31
Book 2017 nervous systems. .MicroRNA Technologies .also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression 作者: 輕而薄 時(shí)間: 2025-3-30 15:41
MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and T and dual luciferase assay. Despite the inherent differences between cultures, we were able to consistently show that 3D topography promotes differentiation of hNSCs through modulation of miRNAs associated with cell proliferation and maintenance of stemness.作者: 思想靈活 時(shí)間: 2025-3-30 18:32
Isolating and Screening Subcellular miRNAs in Neuron,r distribution in neuron varies. This suggests that there are cellular mechanisms actively regulating miRNA expression and localization in subcellular compartments of neurons. In this chapter, we review currently available methods of synaptic miRNA profiling from isolating samples, purifying RNAs, and measuring expression.作者: 值得 時(shí)間: 2025-3-30 21:12 作者: grenade 時(shí)間: 2025-3-31 03:53
Measuring miRNA Mediated Translational Regulation with Live Cell Imaging,ry after photobleaching (FRAP) has been widely used to measure local protein synthesis rate in axons. This technique allows us to measure speed and efficiency of translation of certain mRNAs. We modified this method to measure how miRNAs influence on local synthesis of proteins in neurons.作者: 洞察力 時(shí)間: 2025-3-31 06:17
Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord,specific cell types sheds light on differential regulation of their expression and on the potential functional roles of miRNAs in the brain. In this chapter, we present a simple and robust protocol for the detection of miRNAs by in situ hybridization in formalin-fixed paraffin-embedded spinal cords of mature mice and developing embryos.作者: 內(nèi)疚 時(shí)間: 2025-3-31 10:36 作者: nocturia 時(shí)間: 2025-3-31 13:54
0893-2336 tion advice from the experts.This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. .MicroRNA Technologies .also explores newly established techniques to study the role of miRNAs in the ne作者: crescendo 時(shí)間: 2025-3-31 19:18 作者: Suppository 時(shí)間: 2025-4-1 01:11
