作者: forestry 時(shí)間: 2025-3-21 23:41 作者: innovation 時(shí)間: 2025-3-22 03:19
Jens Heyn,Ludwig Christian Hinske,Carola Ledderose,Elisabeth Limbeck,Simone Krethnt for the success of a product, they are not the sole domainsin realizing its competitive edge. Improved manufacturing andoperating quality and performance coupled with reduced acquisitioncost and in-service cost of ownership are important in achievingbusiness success. It is the early phase of desi作者: Dissonance 時(shí)間: 2025-3-22 06:02
Jianzhen Xu PhD,Chi-Wai Wongies of raw materials maintenance and replacement of worn tools. When any part of the system changes from state of functioning.to a state of failure.the system loses a certain amount of functionality. Restoration of the system’s functionality is invariably achieved through maintenance. And, all maint作者: 天氣 時(shí)間: 2025-3-22 11:05
Botao Zhao,Bing Huang,Wei Li,Youxin Jin systems with requirements in the areas of safety and reliability - especially for distributed, real-time and embedded systems. Thus, much research work is under progress in these fields, since software process improvement impinges directly on achieved levels of quality, and many application experim作者: 熱情的我 時(shí)間: 2025-3-22 16:27
Yi Jin,Zujian Chen,Xiqiang Liu,Xiaofeng Zhou. Depending on personal goals, interests and background, the interpretation of the reliability concept is, different per individual. Industrial organisations try to fulfil user-needs regarding reliability but those reliability requirements are not as unambiguous as they should be. Therefore most ind作者: dry-eye 時(shí)間: 2025-3-22 20:47 作者: Flirtatious 時(shí)間: 2025-3-22 22:54 作者: 使厭惡 時(shí)間: 2025-3-23 02:16
Abdol Hossein Rezaeian,Yuan Gao,Hui-Kuan Lin PhDith requirements in the areas of safety and reliability - especially for distributed, real-time and embedded systems. Thus, much research work is under progress in these fields, since software process improvement impinges directly on achieved levels of quality, and many application experiments aim t作者: Affirm 時(shí)間: 2025-3-23 09:36 作者: 主動(dòng) 時(shí)間: 2025-3-23 11:53
Scott L. Pratt,T. Ashley Burns,Marcy D. Owens,Susan K. Duckett available information. This has made the World Wide Web one of the key fields for companies advertisement. On the other hand, advertisement on the web depends crucially on its ., i.e. on the possibility to be noticed by as many users as possible. The backbone of information management in the WWW is作者: 咽下 時(shí)間: 2025-3-23 15:33
Ping Wu,Melissa A. Wilmarth,Feng Zhang,Guangwei Du simultaneous failure of ECCS and containment isolation failure) in typical 220 MWe Indian PHWR is simulated in this study to know the impact of accident. The principal phenomenon for atmospheric transport of effluent is considered using a Gaussian plume model, MUSEMET which takes hourly variation o作者: prodrome 時(shí)間: 2025-3-23 18:28
Shi-Lung Lin,Shao-Yao Ying all plant operating states. Shutdown Probabilistic Safety Assessment (SPSA) is a systematic methodology for assessment of the safety of nuclear power plant at shutdown mode of operation. A comprehensive Shutdown PSA has been conducted for a typical boiling water reactor (BWR) to demonstrate the saf作者: Allure 時(shí)間: 2025-3-24 00:42
Angeliki Pantazi,Athanasios Zovoilislability of offsite power, by having diverse route for two independent offsite power buses. Three emergency diesel generators (EDGs) of 100% capacity for a twin-unit station have been provided along with a dedicated station blackout diesel generator (SBO DG) with 100% capacity, which will provide a 作者: Charitable 時(shí)間: 2025-3-24 02:35
Amit Sharma,Joseph C. Wug and brownfield deployment on an existing interlocking infrastructure. Octys is already in operation on several metro lines in Paris, and RATP plans its deployment on several other lines in the forthcoming years. Besides the size and complexity of the system, the main technical challenges of the an作者: Friction 時(shí)間: 2025-3-24 10:13
The MicroRNA,n based on sequence complementarity between the miRNA and its targeted mRNA. Different names have been used to describe various types of miRNA. During evolution, RNA retroviruses or transgenes invaded the eukaryotic genome and inserted itself in the noncoding regions of DNA, conceivably acting as tr作者: Circumscribe 時(shí)間: 2025-3-24 11:54 作者: Obloquy 時(shí)間: 2025-3-24 14:54 作者: 貧困 時(shí)間: 2025-3-24 20:33 作者: 刺激 時(shí)間: 2025-3-24 23:22 作者: 天文臺(tái) 時(shí)間: 2025-3-25 03:51 作者: ANIM 時(shí)間: 2025-3-25 08:47 作者: Chipmunk 時(shí)間: 2025-3-25 14:32 作者: 拋物線 時(shí)間: 2025-3-25 19:52
MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells,ls. Here, we describe an miRNA microarray method to monitor the miRNA expression profiles during the course of P19 cells neuronal differentiation. The results indicated that there were significant differences between the miRNA expression in P19 EC cells and the resultant differentiated neural stem c作者: Initiative 時(shí)間: 2025-3-25 22:45 作者: 飛鏢 時(shí)間: 2025-3-26 03:09
Expression Pattern Analysis of MicroRNAs in ,,nscriptional level by basepairing with target messenger RNA and blocking its translation or inducing its degradation. miRNAs play important roles in a variety of biological processes, including development, proliferation, differentiation, cell fate determination, apoptosis, signal transduction, host作者: 挑剔小責(zé) 時(shí)間: 2025-3-26 06:45
Use of Viral Systems to Study miRNA-Mediated Regulation of Gene Expression in Human Cells, viruses also encode miRNAs and these viral miRNAs target cellular and viral mRNAs to regulate virus replication and latent infection. In particular, herpesviruses encode a large number of miRNAs. Herpesvirus infection also changes the normal expression profile of cellular miRNAs. New genetic tools 作者: 作繭自縛 時(shí)間: 2025-3-26 10:00
Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Celof endogenous small RNAs have advanced our knowledge in cancer progression and metastasis. Among many tools, small RNA cloning is a powerful method to identify new microRNAs (miRNAs) and to profile miRNA expression and function. Retroviral system is also the minimum requirement for the studying of m作者: 記憶 時(shí)間: 2025-3-26 16:15
Preparing Synaptoneurosomes from Adult Mouse Forebrain, of synaptic membranes on sucrose or Percoll gradients. Alternatively, synaptoneurosomes are prepared by filtration of tissue homogenate through a series of filters to obtain a fraction that is enriched in pinched-off dendritic spines. Whereas the protocol for making synaptosomes is reasonably well 作者: Sarcoma 時(shí)間: 2025-3-26 18:07 作者: Ballerina 時(shí)間: 2025-3-26 22:28
miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing,s triggered by double-stranded small interfering RNAs, which can be processed from naturally or artificially expressed microRNAs (miRNAs) or small hairpin RNAs (shRNAs). The development of reliable RNAi vectors encoding artificial and natural miRNAs would be useful tools for basic research and thera作者: 單色 時(shí)間: 2025-3-27 05:10 作者: Overdose 時(shí)間: 2025-3-27 08:26 作者: Resign 時(shí)間: 2025-3-27 09:26 作者: 種植,培養(yǎng) 時(shí)間: 2025-3-27 13:52
Ludwig Christian Hinske,Jens Heyn,Pedro A. F. Galante,Lucila Ohno-Machado,Simone Krethof dislocations within the crystaline lattice, the fracture of polymeric bonds in the material, the microscopic extension of a fatigue crack in some metallic sub-component, the abrasion or galling of some surface or any other form of deterioration for which there is no self-recovery or healing. The 作者: Kidnap 時(shí)間: 2025-3-27 18:12
Chih-Hao Kuo,Mark D. Goldberg,Shi-Lung Lin,Shao-Yao Ying,Jiang F. Zhongust operate; the owner-operator of the system has needfully operated it outside its design envelope; the manufacturer failed to eliminate minor defects from the system either during construction or inspection; the supplier has substituted inferior material, causing inherent weaknesses in a component作者: intricacy 時(shí)間: 2025-3-28 00:46 作者: graphy 時(shí)間: 2025-3-28 02:14
Jianzhen Xu PhD,Chi-Wai Wong oil pressure warning lights, magnetic oil filter plugs through to highly complex equipment such as engine health and usage monitoring systems.these all play their part in allowing the operator to decide the best time to perform preventive maintenance.作者: 壓迫 時(shí)間: 2025-3-28 07:32
Yi Jin,Zujian Chen,Xiqiang Liu,Xiaofeng Zhouvolved is therefore enabled. Based on consensus the appropriate measures can be selected. The model should operationalise the basic relation between process and product, it enables the definition of metrics to evaluate process improvements, and links engineering activities to user requirements. The 作者: 喪失 時(shí)間: 2025-3-28 11:57
Meltem Isik,Eugene Berezikovvolved is therefore enabled. Based on consensus the appropriate measures can be selected. The model should operationalise the basic relation between process and product, it enables the definition of metrics to evaluate process improvements, and links engineering activities to user requirements. The 作者: PARA 時(shí)間: 2025-3-28 18:12
Eleonora Forte,Micah A. Luftigvolved is therefore enabled. Based on consensus the appropriate measures can be selected. The model should operationalise the basic relation between process and product, it enables the definition of metrics to evaluate process improvements, and links engineering activities to user requirements. The 作者: 價(jià)值在貶值 時(shí)間: 2025-3-28 18:59 作者: Perennial長(zhǎng)期的 時(shí)間: 2025-3-29 02:44 作者: Blasphemy 時(shí)間: 2025-3-29 05:09
Scott L. Pratt,T. Ashley Burns,Marcy D. Owens,Susan K. Duckettke them rank higher: this relatively new phenomenon (called ., after search engine .ersuasion) has now become so widespread to be a great problem: Indeed, on the one hand it provokes a great loss in advertisement profits for search engines maintainers, which are unwillingly giving free advertisement作者: irritation 時(shí)間: 2025-3-29 10:12
Ping Wu,Melissa A. Wilmarth,Feng Zhang,Guangwei Dut of emergency response planning, affected sector and area are determined under variety of meteorological conditions. The protection offered by iodine prophylaxis and sheltering has been emphasized in this study. Sheltering can be effective by keeping the public out of the plume exposure pathway dur作者: 很是迷惑 時(shí)間: 2025-3-29 13:38
Shi-Lung Lin,Shao-Yao Yingplant operating states. To reduce the effects of dynamic nature, refueling shutdown (RSD) duration is divided into 7 plant operating states (POS) and plant configuration is assumed constant during each POS. Six transients are considered as initiating events (IEs) in this study. For each IE, a separa作者: 相反放置 時(shí)間: 2025-3-29 17:32
Angeliki Pantazi,Athanasios Zovoilist progression of SBO is highly influenced by the EC operation/availability. This paper discusses the results obtained from study of postulated station blackout (SBO) scenarios with both the availability and unavailability of EC in TAPS-1&2. Following SBO, emergency condenser (EC) acts as heat sink b作者: overhaul 時(shí)間: 2025-3-29 20:24
Amit Sharma,Joseph C. Wuwe obtain a rigorous mathematical proof of the safety at system level—a level that is usually covered by informal reasoning and domain expert knowledge only. Such proof is thus feasible and it brings to light and precisely records the knowledge and know-how of the domain experts that have designed t作者: Paleontology 時(shí)間: 2025-3-30 03:12
The MicroRNA,e in embryonic development. They might play a pivotal role in diverse biological systems in various organisms, facilitating a quick response and accurate plotting of body physiology and structures. Based on these unique properties, manufactured intronic miRNAs have been developed for in vitro evalua作者: MANIA 時(shí)間: 2025-3-30 05:42 作者: Muffle 時(shí)間: 2025-3-30 08:29
Identify Intronic MicroRNA with Bioinformatics,n developed to identify novel miRNA. In human miRNA study, many studies search for the mostly complete human genomic sequence. Here, we report a rapid bioinformatics approach to mine miRNA from gene specific introns. Intronic miRNA may directly regulate the expression of its target genes during deve作者: Fabric 時(shí)間: 2025-3-30 15:58
Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Celaintain the necessary motifs of native enhancer for enhancement of mature miRNA expression. Furthermore, the procedure eliminates miRNA extraction and cDNA synthesis before cloning and sequential cloning of more than one miRNA makes this protocol cost- and time-effective to eliminate many frequent c作者: Neuropeptides 時(shí)間: 2025-3-30 19:20
miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing, this chapter the protocols for cloning artificial and natural miRNAs (or shRNAs), evaluating their efficiency in downregulating gene expression, and also discuss the potential applications of these vectors.作者: Ingratiate 時(shí)間: 2025-3-30 21:02
Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs,est in clinical and circumstantial malfunction; for example, type 2 myotonic dystrophy and fragile X syndrome were found to be associated with miRNAs derived from introns. Intronic miRNA is a new class of miRNAs derived from the processing of nonprotein-coding regions of gene transcripts. The intron作者: insular 時(shí)間: 2025-3-31 01:13 作者: gerrymander 時(shí)間: 2025-3-31 06:04
Botao Zhao,Bing Huang,Wei Li,Youxin Jinware that safety is an issue. Therefore, there is a growing evidence for encouraging greater understanding of safety and reliability requirements issues, right 978-1-4757-6399-7978-0-387-35097-4Series ISSN 1868-4238 Series E-ISSN 1868-422X 作者: 漂白 時(shí)間: 2025-3-31 12:22
1064-3745 ghly successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible labora978-1-4939-6261-7978-1-62703-083-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 現(xiàn)暈光 時(shí)間: 2025-3-31 17:10 作者: 我不死扛 時(shí)間: 2025-3-31 19:12 作者: Sedative 時(shí)間: 2025-3-31 22:29
Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer,d RNA and visualization of Dicer cleavage products by autoradiography. The point-by-point steps of substrate preparation, recombinant Dicer cleavage assay, and northern blotting are described in this manuscript.作者: 繞著哥哥問(wèn) 時(shí)間: 2025-4-1 02:29
Enrichment Analysis of miRNA Targets,ational tools in a step-by-step manner. Important details are also provided to help researchers choose more appropriate tools for a given type of analysis. Available Web-based resources for enrichment analysis of miRNA targets are summarized.作者: hereditary 時(shí)間: 2025-4-1 08:20
Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay,understanding microRNA functions. The luciferase reporter gene assay has recently been adapted to test the effect of microRNAs. In this chapter, we use a previously identified miR-138 targeting site in the 3′-untranslated region (3′-UTR) of the RhoC mRNA as an example to describe a quick method for testing the interaction of microRNA and mRNA.作者: micturition 時(shí)間: 2025-4-1 10:23
