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標題: Titlebook: Methods in Protein Structure Analysis; M. Zouhair Atassi,Ettore Appella Book 1995 Springer Science+Business Media New York 1995 Amino acid [打印本頁]

作者: 宣告無效    時間: 2025-3-21 18:32
書目名稱Methods in Protein Structure Analysis影響因子(影響力)




書目名稱Methods in Protein Structure Analysis影響因子(影響力)學科排名




書目名稱Methods in Protein Structure Analysis網(wǎng)絡公開度




書目名稱Methods in Protein Structure Analysis網(wǎng)絡公開度學科排名




書目名稱Methods in Protein Structure Analysis被引頻次




書目名稱Methods in Protein Structure Analysis被引頻次學科排名




書目名稱Methods in Protein Structure Analysis年度引用




書目名稱Methods in Protein Structure Analysis年度引用學科排名




書目名稱Methods in Protein Structure Analysis讀者反饋




書目名稱Methods in Protein Structure Analysis讀者反饋學科排名





作者: Nonflammable    時間: 2025-3-21 22:20
A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vapord endoprotease contamination. Several chemical degradation methods have been reported (Stark, 1968; Yamashita,1971; Bailey et al., 1994), and a few automated C-terminal sequencers are almost available to the public.
作者: 朦朧    時間: 2025-3-22 00:59
Automated C-Terminal Sequencing of Polypeptides Containing C-Terminal Proline methods have been described, the method known as the “thiocyanate method” (Schlack and Kumpf, 1926), has been the most widely studied and appears to offer the most promise due to its similarity to current methods of N-terminal sequence analysis.
作者: nascent    時間: 2025-3-22 05:19

作者: 公式    時間: 2025-3-22 08:58
with emphasis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of the lOth MPSA decided to change the name to Methods in Protein Structu
作者: consolidate    時間: 2025-3-22 15:55
Book 1995sis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of the lOth MPSA decided to change the name to Methods in Protein Structure Analysi
作者: 換話題    時間: 2025-3-22 17:49
Pre-Electrophoretic Labelling of Proteins with a Coloured Water Soluble Edman Reagente peptides eluted for subsequent HPLC purification (Bauw 1989). Both these procedures require the proteins to be stained after electrophoresis in order to locate their position in the gel or on the membrane. The staining process generally fixes the proteins and leads to significant loss of material.
作者: parsimony    時間: 2025-3-23 00:21
https://doi.org/10.1007/978-1-4899-1031-8Amino acid; chemistry; preparation; protein; sequence analysis
作者: Prophylaxis    時間: 2025-3-23 03:00
High Sensitivity Analysis of PTH Amino AcidsCapillary electrophoresis and laser-based photothermal detection are used to analyze minute amounts of PTH amino acids. This technology is demonstrated for analysis of manual Edman degradation reactions. This technology is also used to analyze the products generated by a highly miniaturized automated protein sequencer.
作者: FADE    時間: 2025-3-23 08:50

作者: 文藝    時間: 2025-3-23 10:28
978-1-4899-1033-2Springer Science+Business Media New York 1995
作者: ARCHE    時間: 2025-3-23 17:45

作者: 做事過頭    時間: 2025-3-23 19:05

作者: LIMIT    時間: 2025-3-24 01:11

作者: RAG    時間: 2025-3-24 05:59
Victoria L. Boyd,MeriLisa Bozzini,Jindong Zhao,Robert J. DeFranco,Pau-Miau Yuan,G. Marc Loudon,Duy N
作者: CHOIR    時間: 2025-3-24 06:42

作者: 的闡明    時間: 2025-3-24 11:08

作者: 上流社會    時間: 2025-3-24 18:12
D. J. C. Pappin,D. Rahman,H. F. Hansen,W. Jeffery,C. W. Sutton
作者: 不要嚴酷    時間: 2025-3-24 22:03
n open-source degradation simulator for linear systems. This simulator can simulate the degradation in closed-loop machines whose dynamics are known. It is also possible to simulate different degradation models for different system parts simultaneously by adding different processes and output noise
作者: cluster    時間: 2025-3-25 02:56
n open-source degradation simulator for linear systems. This simulator can simulate the degradation in closed-loop machines whose dynamics are known. It is also possible to simulate different degradation models for different system parts simultaneously by adding different processes and output noise
作者: Airtight    時間: 2025-3-25 04:00

作者: 不給啤    時間: 2025-3-25 11:31

作者: 態(tài)度暖昧    時間: 2025-3-25 15:02
Robert L. Moritz,James Eddes,Richard J. Simpson to reveal its intrinsic properties by using nonparametric methods. This volume contains a selection of contributions presented at the XVIII Annual Congress of the Portuguese Statistical Society..978-3-642-42972-9978-3-642-32419-2Series ISSN 2194-7767 Series E-ISSN 2194-7775
作者: 間接    時間: 2025-3-25 19:25

作者: nostrum    時間: 2025-3-25 20:02
nstrate that the entire family is a flexible device that adapts to many interesting situations of modern scientific practice where the number of observations stays fixed or grows very slowly while the number of automatically measured features grows dramatically and only a small fraction of these fea
作者: chassis    時間: 2025-3-26 02:44

作者: instate    時間: 2025-3-26 04:50
High-Speed Chromatographic Separation of Proteins and Peptides for High Sensitivity Microsequence Anfluoride (Fernandez et al., 1992) and nitrocellulose (Aebersold et al., 1987), following electrotransfer from the gel. Protein identification can be achieved by microsequence analysis of the isolated peptides using either automated Edman degradation or tandem mass spectrometry (Hunt et al., 1986; Bu
作者: 使服水土    時間: 2025-3-26 09:19
Synthesis, Evaluation and Application of a Panel of Novel Reagents for Stepwise Degradation of Polyp et al, 1990, 1991; Aebersold et al, 1991; Gooley et al, 1991; Pisano et al, 1993), modified and unnatural amino acids of known structure are difficult to identify and de-novo characterization of such residues by UV absorbance detection alone is extremely difficult.
作者: 彩色的蠟筆    時間: 2025-3-26 13:07

作者: 束縛    時間: 2025-3-26 18:55
Deacetylation and Internal Cleavage of Polypeptides for N-Terminal Sequence Analysision, long handling times and inaccessibility of the N-terminal fragment to Edman degradation, are avoided. The protocol has been applied to both a synthetic peptide corresponding to the N-terminal segment of horse liver alcohol dehydrogenase and to the intact protein.
作者: Override    時間: 2025-3-26 21:52
Sequencing of Proteins from the C-Terminusdetect that residue when it is present in one nanomole of a protein sample. On average, it is possible to sequence 5 cycles on one nanomole of protein applied to polyvinylidene difluoride (PVDF) membrane if the amino acid sequence contains those residues listed in the “reliably called” column of Tab
作者: 昆蟲    時間: 2025-3-27 01:43
Tandem Mass Spectrometric Characterization of Modified Peptides and Proteinsdem MS of peptides using four-sector mass spectrometers generally involves high energy collisional activation of precursor ions (usually [M+H].) to promote fragmentations indicative of sequence and permitting the differentiation of isomeric/isobaric amino acid residues [1].
作者: gout109    時間: 2025-3-27 08:22
Direct Mass Spectrometric Analyses for Protein Chemistry StudiesMass spectrometry is a sensitive bioanalytical method (McCloskey, 1990), but it is often difficult for the method to selectively discriminate against . species found in a sample, in search of the few components the scientist is truly interested. MS analysis of a sample containing a small amount of p
作者: 男學院    時間: 2025-3-27 13:07
Peptide-Mass Fingerprinting as a Tool for the Rapid Identification and Mapping of Cellular Proteinsificant analytical challenges. The recent introduction of matrix-assisted laser-desorption (MALD) time-of-flight mass spectrometers (Karas and Hillenkamp, 1988) has led to the rapid analysis (at high sensitivity) of peptide mixtures. New strategies have been developed using a combination of protease
作者: 橫條    時間: 2025-3-27 15:26

作者: GILD    時間: 2025-3-27 18:32
ers, based on scientific standing and activity. The chairman, subject to approval of the Scientific Advisory Committee, appoints the Organizing Committee. It is this latter committee that puts the conference together. The lectures of the MPSA have traditionally been published in a special proceedings issue. T978-1-4899-1033-2978-1-4899-1031-8
作者: 千篇一律    時間: 2025-3-28 00:42
Characterization of Proteins Separated by Gel Electrophoresis at the Primary Structure Leveln the focus on complex, highly regulated systems consisting of numerous interacting elements. A complete understanding of such processes can only be achieved if the problem is approached globally, considering the temporal and spatial interactions of all the elements involved. This task is supported
作者: 聲音刺耳    時間: 2025-3-28 02:36
New Strategies in High Sensitivity Characterization of Proteins Separated from 1-D or 2-D Gelsom very complex mixtures (O’Farrell, 1975; Celis & Bravo, 1984). For structural analysis, proteins are electrotransferred from the gels onto immobilizing membranes for subsequent NH.-terminal sequence analysis (Vandekerckhove .., 1985; Aebersold .., 1986; Matsudaira, 1987). Alternatively, proteins c
作者: 使饑餓    時間: 2025-3-28 06:38

作者: 信徒    時間: 2025-3-28 11:02
Pre-Electrophoretic Labelling of Proteins with a Coloured Water Soluble Edman Reagentof proteins. However, it is generally used as an analytical rather than a preparative tool. With the advent of PVDF membranes which are stable under the conditions employed by the Edman degradation, it has become common to try and obtain N-terminal sequence data from proteins separated by SDS PAGE f
作者: BRUNT    時間: 2025-3-28 14:49
Synthesis, Evaluation and Application of a Panel of Novel Reagents for Stepwise Degradation of Polypf proteins and peptides. As a benefit of this distinction, over the last four decades the method has been refined to a high degree of perfection. Nevertheless, sequencing with phenyl isothiocyanate (PITC). suffers from a few practical limitations. First, the extinction coefficient of the phenylthioh
作者: 障礙物    時間: 2025-3-28 19:39

作者: microscopic    時間: 2025-3-29 01:03

作者: TIGER    時間: 2025-3-29 06:47
A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vaporynthesis of immunoreactive peptides. To date, amino(N)-terminal sequencing using the Edman degradation procedure has almost exclusively provided such data. Methodologies for sequencing proteins from their carboxy(C)-termini have remained relatively primitive requiring much protein in return for litt
作者: duplicate    時間: 2025-3-29 10:15
Sequencing of Proteins from the C-Terminus our investigations, including the mechanism of the initial activation of the C-terminal carboxyl group and the deliberate modifications of the reactive side-chains of the amino acids with the sequencing reagents. Through the selection of the reagents and reaction conditions used for our sequencing
作者: cliche    時間: 2025-3-29 13:52

作者: 包裹    時間: 2025-3-29 18:47
Automated C-Terminal Sequencing of Polypeptides Containing C-Terminal Prolinemethod would be analogous and complimentary to the Edman degradation commonly used for N-terminal sequence analysis (Edman, 1950). It would also be invaluable for the sequence analysis of proteins with naturally occurring N-terminal blocking groups, for the detection of post-translational processing
作者: fluffy    時間: 2025-3-29 22:55
Tandem Mass Spectrometric Characterization of Modified Peptides and Proteinsture determination. The value of MS derives from the accommodation of mixtures and the possibilities for characterization of modified amino acid residues. Tandem MS in particular is important in addressing both of these issues. The essential features of tandem MS are the promotion of ion fragmentati
作者: finale    時間: 2025-3-30 02:50
Direct Mass Spectrometric Analyses for Protein Chemistry Studies laboratory. Protein sequencing methods and mass spectrometry are often plagued with the same problems in that samples are frequently contaminated with other materials. Many buffer components, salts, and solubilizing detergents can interfere or prevent the successful application of both methodologie
作者: instate    時間: 2025-3-30 06:21

作者: 下垂    時間: 2025-3-30 09:08
st reduction. Using the available data with different machine learning methods, especially deep learning methods, the accuracy of estimation and prediction of faults and RUL have increased dramatically. However, due to the statistical nature of the machine learning methods and the limitations of ava
作者: faddish    時間: 2025-3-30 12:32

作者: Horizon    時間: 2025-3-30 17:20
Ruedi Aebersold,Lawrence N. Amankwa,Heinz Nika,David T. Chow,Edward J. Bures,Hamish D. Morrison,Daniel. Three different methodologies are applied with that purpose: (1) polls aggregation, using existing auxiliary polling; (2) application of multilevel regression methods, using the multilevel structure of the data; and (3) methods of small area estimation, making use of auxiliary information throug




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