標(biāo)題: Titlebook: Methods for Fragments Screening Using Surface Plasmon Resonance; Sameer Mahmood Zaheer,Ramachandraiah Gosu Book 2021 The Editor(s) (if app [打印本頁] 作者: 聲音會爆炸 時間: 2025-3-21 17:49
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance影響因子(影響力)
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance影響因子(影響力)學(xué)科排名
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance網(wǎng)絡(luò)公開度
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance被引頻次
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance被引頻次學(xué)科排名
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance年度引用
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance年度引用學(xué)科排名
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance讀者反饋
書目名稱Methods for Fragments Screening Using Surface Plasmon Resonance讀者反饋學(xué)科排名
作者: 受人支配 時間: 2025-3-21 20:46 作者: 清澈 時間: 2025-3-22 03:24
Preparation of Protein with Histidine Tag for Amine Couplingg with a relevant tag is critical. These tags can be used for ligating the protein onto the sensor with specific sensor chemistry. This chapter will outline the preparation of histidine tagged protein and can be used for the amine coupling technique.作者: BRINK 時間: 2025-3-22 06:58 作者: OCTO 時間: 2025-3-22 12:35
Protein Stability Using Thermal Shift Assay (TSA): pH Toleranceto the sensors, protein of interest need to be evaluated for pH tolerability and stability. Buffer mismatch is an important aspect of SPR assay due to its high sensitivity for small changes. Protein storage buffer is relatively different from immobilization and running buffer. To perform amine coupl作者: 字形刻痕 時間: 2025-3-22 14:08
Protein Stability Using Thermal Shift Assay (TSA): DMSO Toleranceto the sensors, protein of interest need to be evaluated for DMSO tolerability and stability. Buffer composition is an important aspect of SPR assay due to its high sensitivity for small changes in refractive indices. DMSO is one of the major factors that influence buffer mismatch leading to failure作者: osculate 時間: 2025-3-22 17:17
Selection and Identification of Fragment Librarys essential for starting fragment-based drug discovery efforts as multiple libraries are available commercially built on different clustering criteria, which are redundant to an extent. Although many criteria can be applied to select a diverse set of fragments toward specific targets, we describe ap作者: 食道 時間: 2025-3-22 21:34
Preparation of SPR Sensorstrument. Improper sensitization or activation of biosensors can lead to nonuniform ligation of ligands, which result in variations between triplicates during SPR assays. Inadequate activation is also responsible for the ligand drift post immobilization. This chapter provides information to prepare 作者: Latency 時間: 2025-3-23 01:25 作者: 袋鼠 時間: 2025-3-23 08:48 作者: 擴(kuò)張 時間: 2025-3-23 13:14
Preparation of Fragments for Screening in SPRe for successfully screening small molecule fragments lie with the proper sample preparation before injecting into the SPR system. One of the major limitations of the HTS campaign using SPR with small molecules is solubility. Fragments are more soluble than small molecules. However, if even one or a作者: obsolete 時間: 2025-3-23 17:11
Identification of Fragment Hitssponse curves for kinetic and affinity analysis. The user-friendly interface guides the user through a series of data transformations that produce data sets that are then fitted with standard kinetic or affinity models. A key feature is the ability to rapidly perform single or double referencing of 作者: 你敢命令 時間: 2025-3-23 20:29 作者: ALT 時間: 2025-3-24 00:16
Ramachandraiah Gosu,Sameer Mahmood Zaheergemony’ in South America, sub-Saharan Africa, Eurasia, SouthWhen do rising powers fail to establish legitimate regional leadership and instead face contestation by their regional challengers? This book investigates how and why the BRICS (Brazil, Russia, India, China, and South Africa) project leader作者: SOW 時間: 2025-3-24 05:39
gemony’ in South America, sub-Saharan Africa, Eurasia, SouthWhen do rising powers fail to establish legitimate regional leadership and instead face contestation by their regional challengers? This book investigates how and why the BRICS (Brazil, Russia, India, China, and South Africa) project leader作者: acquisition 時間: 2025-3-24 08:31 作者: 易受刺激 時間: 2025-3-24 12:49
Saravanan Kandan,Dinesh Thiagaraj,Rashmi Rekha Devigemony’ in South America, sub-Saharan Africa, Eurasia, SouthWhen do rising powers fail to establish legitimate regional leadership and instead face contestation by their regional challengers? This book investigates how and why the BRICS (Brazil, Russia, India, China, and South Africa) project leader作者: expeditious 時間: 2025-3-24 18:51
gemony’ in South America, sub-Saharan Africa, Eurasia, SouthWhen do rising powers fail to establish legitimate regional leadership and instead face contestation by their regional challengers? This book investigates how and why the BRICS (Brazil, Russia, India, China, and South Africa) project leader作者: 我悲傷 時間: 2025-3-24 23:00
Srinivasan Swaminathan,A. L. Theerthangemony’ in South America, sub-Saharan Africa, Eurasia, SouthWhen do rising powers fail to establish legitimate regional leadership and instead face contestation by their regional challengers? This book investigates how and why the BRICS (Brazil, Russia, India, China, and South Africa) project leader作者: Crayon 時間: 2025-3-25 02:05 作者: 破布 時間: 2025-3-25 04:34
economic, social, and political pressure on the state, the Kingdom remains immune from the surprisingly widespread political upheavals; this immunity reveals mainly that the Saudi state’s sources of legitimacy are deeper than is usually conceived abroad. To put things into a wider context, changes w作者: Salivary-Gland 時間: 2025-3-25 11:01
Rajendra Kristam,Ramachandraiah Gosuuld even call it an aggressive role as a regional player in the Middle East. In relative contrast to the first decade of the twenty-first century, when many observers have lamented about a largely stagnant foreign policy decision-making process in Riyadh, pointing to the overly gerontocratic structu作者: facilitate 時間: 2025-3-25 13:57 作者: Decrepit 時間: 2025-3-25 16:19 作者: 針葉 時間: 2025-3-25 23:58 作者: ticlopidine 時間: 2025-3-26 03:06
Sameer Mahmood Zaheer,Aswathy Pillaieconomic, social, and political pressure on the state, the Kingdom remains immune from the surprisingly widespread political upheavals; this immunity reveals mainly that the Saudi state’s sources of legitimacy are deeper than is usually conceived abroad. To put things into a wider context, changes w作者: Affection 時間: 2025-3-26 04:52
Sameer Mahmood Zaheer,Aswathy Pillaiuld even call it an aggressive role as a regional player in the Middle East. In relative contrast to the first decade of the twenty-first century, when many observers have lamented about a largely stagnant foreign policy decision-making process in Riyadh, pointing to the overly gerontocratic structu作者: 抒情短詩 時間: 2025-3-26 12:19 作者: 阻塞 時間: 2025-3-26 15:54
Sameer Mahmood Zaheer,Ramachandraiah Gosuwith other countries. MEs as well as PEs, and that they bear only a tenuous relationship with domestic prices in any of the CMEA members. At the very least, this situation is highly paradoxical when placed against the backdrop of the time and effort devoted to the gradual elaboration of common TRP f作者: prosthesis 時間: 2025-3-26 17:28
with other countries. MEs as well as PEs, and that they bear only a tenuous relationship with domestic prices in any of the CMEA members. At the very least, this situation is highly paradoxical when placed against the backdrop of the time and effort devoted to the gradual elaboration of common TRP f作者: 構(gòu)想 時間: 2025-3-27 00:47
https://doi.org/10.1007/978-981-16-1536-8Surface plasmon resonance; Protein stability; Fragment library; Thermal shift assay; Amine coupling作者: conservative 時間: 2025-3-27 03:52 作者: Reverie 時間: 2025-3-27 08:18 作者: 遍及 時間: 2025-3-27 10:15
Preparation of Protein with AviTag? for Biotin-Based Capturelecules by avidin–biotin-based methods is very common due to the robustness of the interaction. Preparation of AviTag? protein for SPR is the primary work to initiate the binding studies. This chapter will outline the preparation of AviTag? protein.作者: –LOUS 時間: 2025-3-27 14:58
Sameer Mahmood Zaheer,Ramachandraiah GosuDiscusses real-time and label-free measurement of biomolecular interaction.Present protocols to calculate kinetics and affinities with single analyte concentration.Examines techniques for the optimum 作者: cocoon 時間: 2025-3-27 21:31
http://image.papertrans.cn/m/image/632243.jpg作者: FLIP 時間: 2025-3-27 23:41
Screening of Fragments in SPRHigh throughput screening using the SPR system has revolutionized drug discovery in identifying hits. Several SPR platforms are available with their respective software to setup the screening cascade. We used Pioneer software to input various parameters required for a successful screening.作者: Middle-Ear 時間: 2025-3-28 05:03 作者: 天文臺 時間: 2025-3-28 06:49 作者: infantile 時間: 2025-3-28 11:32
Selection and Identification of Fragment Libraryplications of criteria that in general result in the selection of a diverse set of fragments keeping in mind their later expansion into full small molecules and their development into possible drugs with good profiles.作者: 會議 時間: 2025-3-28 17:00
Optimization and Validation of Amine Coupling of Ligande protein is usually easily achieved without denaturation and inactivation of the protein. Optimization and validation protocol is presented here with important criteria and troubleshooting with action taken considered at each step.作者: Asymptomatic 時間: 2025-3-28 21:23
Preparation of Fragments for Screening in SPR small fraction of fragments in a screening plate are less soluble, it can lead to blockage of SPR system capillaries. This chapter provides optimal details required for the preparation of fragments to minimize clogging.作者: 失誤 時間: 2025-3-29 01:50
Data Analysis and Confirmation of Hitsing using SPR. An error at this point in identifying a true hit will have a significant impact on the generation of starting material and mislead medicinal chemists. This final chapter provides in detail, the generation of kinetic parameters of these hits.作者: 牽索 時間: 2025-3-29 05:59 作者: 實(shí)現(xiàn) 時間: 2025-3-29 07:49
Book 2021f the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-st作者: 結(jié)果 時間: 2025-3-29 13:48 作者: Femish 時間: 2025-3-29 16:50 作者: 背心 時間: 2025-3-29 23:47
Optimization and Validation of Strepavidin/Biotin-Based Capture of Ligands chapter highlights the affinity capture (non-covalent) capture method used in SPR assay. The most frequent affinity capture technique is streptavidin-biotin capture. Here we provide the protocol that can guide the optimization and validation of streptavidin-biotin capture method using Biocap (specific to PioneerFE SPR).作者: 老人病學(xué) 時間: 2025-3-30 03:10 作者: 種屬關(guān)系 時間: 2025-3-30 06:50 作者: charisma 時間: 2025-3-30 08:38
Ramachandraiah Gosu,Sameer Mahmood Zaheerive regions that are increasingly pivotal for the emerging world order, the volume offers a global comparative analysis of contemporary regional contested leadership that will interest scholars and students of international affairs, foreign policy, and area studies.?978-3-030-08837-8978-3-319-73691-4作者: menopause 時間: 2025-3-30 14:31 作者: Mediocre 時間: 2025-3-30 20:30
Dinesh Thiagaraj,Ponni Vijayanive regions that are increasingly pivotal for the emerging world order, the volume offers a global comparative analysis of contemporary regional contested leadership that will interest scholars and students of international affairs, foreign policy, and area studies.?978-3-030-08837-8978-3-319-73691-4作者: Hemoptysis 時間: 2025-3-31 00:21
Saravanan Kandan,Dinesh Thiagaraj,Rashmi Rekha Deviive regions that are increasingly pivotal for the emerging world order, the volume offers a global comparative analysis of contemporary regional contested leadership that will interest scholars and students of international affairs, foreign policy, and area studies.?978-3-030-08837-8978-3-319-73691-4
