標題: Titlebook: Metabolic Signaling; Methods and Protocol Sarah-Maria Fendt,Sophia Y. Lunt Book 2019 Springer Science+Business Media, LLC, part of Springer [打印本頁] 作者: 分類 時間: 2025-3-21 18:16
書目名稱Metabolic Signaling影響因子(影響力)
書目名稱Metabolic Signaling影響因子(影響力)學科排名
書目名稱Metabolic Signaling網(wǎng)絡(luò)公開度
書目名稱Metabolic Signaling網(wǎng)絡(luò)公開度學科排名
書目名稱Metabolic Signaling被引頻次
書目名稱Metabolic Signaling被引頻次學科排名
書目名稱Metabolic Signaling年度引用
書目名稱Metabolic Signaling年度引用學科排名
書目名稱Metabolic Signaling讀者反饋
書目名稱Metabolic Signaling讀者反饋學科排名
作者: coddle 時間: 2025-3-21 20:15 作者: 嘮叨 時間: 2025-3-22 01:23 作者: Gerontology 時間: 2025-3-22 07:13 作者: FRAUD 時間: 2025-3-22 08:50 作者: Enrage 時間: 2025-3-22 15:29
Measuring the Nutrient Metabolism of Adherent Cells in Culture,tured adherent mammalian cells to collect both polar and nonpolar intracellular metabolites. This chapter also describes experimental design considerations for performing stable isotope labeling experiments, and the use of chemical derivatization to increase the number of compounds that can be detected using one chromatography method.作者: Flounder 時間: 2025-3-22 18:26
13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells,orage-independent growth, spheroid formation, and extracellular matrix production by (cancer) cells. Here, we describe how to perform metabolomics and .C tracer analysis in 3D cultures of cancer cells.作者: 冥界三河 時間: 2025-3-22 23:28
Book 2019ucible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .?Authoritative and cutting-edge,?.Metabolic Signaling: Methods and Protocols.?aims to provide researchers with methods to study, perturb, and functionally interpret?metabolism and?metabolic signaling from the sub-cellular to the whole-body level..作者: 不知疲倦 時間: 2025-3-23 03:03
Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers,e, but also for the identification of anti-angiogenic metabolic targets. This chapter describes in detail the radioactive tracer substrate assays that have been used for the determination of EC metabolic flux in vitro.作者: 集聚成團 時間: 2025-3-23 08:17
Development of Patient-Derived Tumor Xenograft Models,ed mice, allowing for the progressive growth and amplification of cancer tissue for in vivo testing. Here, we describe the detailed methods we developed to establish PDX from both surgically removed endometrial cancer fragments (endometrial cancer) and fine-needle aspiration biopsies (pancreatic cancer).作者: 六邊形 時間: 2025-3-23 13:25
High-Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry,tography-mass spectrometry (LC-MS) for non-targeted measurement of over 7000 bioactive lipids, including eicosanoids and eicosanoid-related metabolites, in human biosamples. These methods may be applied to the study of population scale cohorts to uncover previously unrecognized roles for bioactive lipid species in human biology.作者: Culpable 時間: 2025-3-23 16:56 作者: 安撫 時間: 2025-3-23 19:40 作者: 閑逛 時間: 2025-3-23 22:27
Assessing the Impact of the Nutrient Microenvironment on the Metabolism of Effector CD8+ T Cells,tudy the impact of the nutrient microenvironment on the metabolism of effector CD8. T cells, based on performing stable .C isotope labeling measurements on in vitro-differentiated murine effector CD8. T cells.作者: 爵士樂 時間: 2025-3-24 02:42
1064-3745 ation advice from the experts.This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling?using experimental and computational approaches.? Chapters detail?protocols to quantify metabolism, i作者: HILAR 時間: 2025-3-24 07:49 作者: 苦惱 時間: 2025-3-24 11:25 作者: 侵略主義 時間: 2025-3-24 16:47
Martin P. Ogrodzinski,Shao Thing Teoh,Lei Yu,Deanna Broadwater,Elliot Ensink,Sophia Y. Lunt作者: 沐浴 時間: 2025-3-24 22:27 作者: 砍伐 時間: 2025-3-25 00:57
Tong Zhang,Christiaan F. Labuschagne,Karen H. Vousden,Oliver D. K. Maddocks作者: 水槽 時間: 2025-3-25 07:16
Yannic Nonnenmacher,Roberta Palorini,Karsten Hiller作者: 抵押貸款 時間: 2025-3-25 11:34
Jessica D. Guillaume,Stephanie L. Celano,Katie R. Martin,Jeffrey P. MacKeigan作者: BROTH 時間: 2025-3-25 12:26 作者: 不在灌木叢中 時間: 2025-3-25 16:54
Fabio Stanchi,Ken Matsumoto,Holger Gerhardt due attention to contraindications and complications. In addition, a novel classification of management parameters is suggested, and a systematic approach to management, formulated. In the final chapter this approach is applied to nine cases that serve as clinical examples.978-3-662-50753-7978-3-642-21840-8作者: 尊敬 時間: 2025-3-25 22:28 作者: Friction 時間: 2025-3-26 02:40 作者: 變異 時間: 2025-3-26 04:24 作者: LEVER 時間: 2025-3-26 10:05
A Protocol to Compare Methods for Untargeted Metabolomics,move the latter from untargeted metabolomic datasets without having to identify metabolite structures. This protocol can be used to compare or optimize methods pertaining to any step of the untargeted metabolomic workflow (e.g., extraction, chromatography, mass spectrometer, informatic software, etc作者: 媒介 時間: 2025-3-26 15:24
Determining the Impact of Metabolic Nutrients on Autophagy,peration with Unc-51 like autophagy activating kinase 1 (ULK1). In this way, a lack of metabolic nutrients plays a critical role in inducing autophagy, while the products of autophagy also serve as readily available fuel for the cell. In this chapter, we describe methods to visualize and quantify au作者: 鳴叫 時間: 2025-3-26 19:25
Determining Macrophage Polarization upon Metabolic Perturbation,ns in macrophages. Here, we describe a basic protocol for examining how glutamine metabolism affects macrophage polarization by using the Extracellular Flux (XF.96) Analyzer (Seahorse Bioscience), which takes real-time measurements of oxidative phosphorylation and glycolysis. We also present a detai作者: Femish 時間: 2025-3-27 00:38
1064-3745 .Metabolic Signaling: Methods and Protocols.?aims to provide researchers with methods to study, perturb, and functionally interpret?metabolism and?metabolic signaling from the sub-cellular to the whole-body level..978-1-4939-9387-1978-1-4939-8769-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 自作多情 時間: 2025-3-27 03:50 作者: ascend 時間: 2025-3-27 07:32
Metabolic Signaling978-1-4939-8769-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: ETHER 時間: 2025-3-27 11:27
https://doi.org/10.1007/978-1-4939-8769-6nutrient metabolism; cancer cells; tumors; deuterium tracing; lipectomized mice作者: 進取心 時間: 2025-3-27 14:22 作者: Ovulation 時間: 2025-3-27 21:27
Measuring Rates of ATP Synthesis,nked to the mitochondrial’ electron transport chain components of your interest being either through complex I (with or without a linkage to pyruvate dehydrogenase activity), through complex II, or through the electron transport flavoprotein and complex I (β-oxidation of fatty acids).作者: 推延 時間: 2025-3-27 23:36
Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism,four simple methods to measure cell death in culture by using microscopy, western blot, and flow cytometry. We also provide tools to differentiate between different forms of cell death like apoptosis and necrosis by using chemical inhibitors.作者: hazard 時間: 2025-3-28 02:29 作者: 報復(fù) 時間: 2025-3-28 07:01 作者: Spinal-Tap 時間: 2025-3-28 10:30
Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/631257.jpg作者: reserve 時間: 2025-3-28 16:55
A Protocol to Compare Methods for Untargeted Metabolomics,d and optimized for a small number of select metabolites, very few have been assessed on the basis of global metabolite coverage. Thus, when performing untargeted metabolomics, researchers often question which combination of extraction techniques, chromatographic separations, and mass spectrometers 作者: CYT 時間: 2025-3-28 22:34 作者: 思想靈活 時間: 2025-3-29 00:11
Measuring the Nutrient Metabolism of Adherent Cells in Culture,using techniques such as liquid chromatography-mass spectrometry (LC-MS). This chapter describes in detail a method for metabolite extraction from cultured adherent mammalian cells to collect both polar and nonpolar intracellular metabolites. This chapter also describes experimental design considera作者: frenzy 時間: 2025-3-29 03:46 作者: guardianship 時間: 2025-3-29 10:18 作者: Oratory 時間: 2025-3-29 13:55 作者: Anthrp 時間: 2025-3-29 15:44 作者: 使激動 時間: 2025-3-29 21:32 作者: Irrigate 時間: 2025-3-30 03:00 作者: linguistics 時間: 2025-3-30 07:05
Determining Compartment-Specific Metabolic Fluxes,cells. Mitochondria of adherent cell cultures are made accessible by the addition of digitonin—a compound that selectively permeabilizes the cytosolic membrane without affecting mitochondrial integrity. The generated in situ mitochondria are subsequently used in a stable isotope labeling assay in wh作者: 疾馳 時間: 2025-3-30 11:03
Determining the Impact of Metabolic Nutrients on Autophagy,tion. However, as a result of increased metabolite consumption and poor vascularization, most cancer cells must survive in a nutrient poor and high cellular stress microenvironment. Cancer cells undergo metabolic reprogramming to evade cell death and ensure proliferation; in particular, cancer cells作者: corpus-callosum 時間: 2025-3-30 16:01 作者: Engulf 時間: 2025-3-30 20:22 作者: elucidate 時間: 2025-3-30 21:32 作者: blight 時間: 2025-3-31 01:09 作者: finite 時間: 2025-3-31 05:50 作者: 搖擺 時間: 2025-3-31 10:54
Lipectomizing Mice for Applications in Metabolism,e adipose tissue, a dynamic tissue that has many biological functions. Specifically visceral adipose tissue (VAT) is an active endocrine organ producing hormones that control systemic metabolism. VAT accumulates immune cells that produce cytokines that drive chronic inflammation and promote insulin 作者: trigger 時間: 2025-3-31 14:05
Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Cultf systemic and local signaling molecules. Here, we describe quantitative multiplex immunoassay analysis of supernatants collected from cultured human bone tissue fragments to profile local factors associated with the bone turnover process.作者: 虛度 時間: 2025-3-31 17:48 作者: preeclampsia 時間: 2025-3-31 22:24
Fabio Stanchi,Ken Matsumoto,Holger Gerhardtlassification of the disease, and suggests a novel systematiThis book, designed for ophthalmologists and particularly for refractive surgeons, aims to develop practical and clinical skills in readers so that they are able to make the right decisions in managing keratoconus. There are four main chapt
