標(biāo)題: Titlebook: Metabolic Reprogramming; Methods and Protocol Salvatore Papa,Concetta Bubici Book 2023 The Editor(s) (if applicable) and The Author(s), und [打印本頁] 作者: Defect 時(shí)間: 2025-3-21 19:03
書目名稱Metabolic Reprogramming影響因子(影響力)
作者: 擴(kuò)音器 時(shí)間: 2025-3-21 23:44 作者: 佛刊 時(shí)間: 2025-3-22 01:24
Measurement of Fatty Acid Oxidation by High-Resolution Respirometry: Special Considerations for Anatissues/fibers are suspended in oxygenated respiration medium within a closed chamber and substrates or inhibitors added in a stepwise manner. The dissolved oxygen concentration decreases as aerobic metabolism in the specimen proceeds, recorded by an oxygen sensor within the chamber to give a quanti作者: 治愈 時(shí)間: 2025-3-22 07:36 作者: 匍匐 時(shí)間: 2025-3-22 12:49 作者: 用肘 時(shí)間: 2025-3-22 13:24 作者: 節(jié)約 時(shí)間: 2025-3-22 17:22 作者: inscribe 時(shí)間: 2025-3-22 22:41 作者: 透明 時(shí)間: 2025-3-23 05:19
Quantification of Intracellular ATP Content in Ex Vivo GC B Cells,changes in the cell, leading to an increase in ATP production to sustain cell proliferation and differentiation. Current methods available to determine the amount of ATP are time-consuming, require extensive sample processing, and need a large amount of starting material. We set up an easy follow-up作者: caldron 時(shí)間: 2025-3-23 07:13
UHPLC-HRMS-Based Analysis of S-Hydroxymethyl-Glutathione, GSH, and GSSG in Human Cells,tasis. This moiety can react with cellular electrophiles such as formaldehyde yielding the compound S-hydroxymethyl-GSH (HSMGSH). HSMGSH is the substrate of the enzyme alcohol dehydrogenase 5 (ADH5) and thus a key intermediate in formaldehyde metabolism. In this work, we describe a method for the ch作者: Gyrate 時(shí)間: 2025-3-23 12:38 作者: 畢業(yè)典禮 時(shí)間: 2025-3-23 16:41
Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells,and hypoxia response. H.O. generation and signaling are highly localized processes. Understanding the dynamics of this molecule inside intact cells with subcompartmental resolution is instrumental to unravel its role in cellular signaling. Different genetically encoded fluorescent sensors have been 作者: Amorous 時(shí)間: 2025-3-23 19:31
Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue,er disease (NAFLD) are available, but they fail to fully recreate the metabolic and cellular features of human disease. Thus, it is imperative to understand the metabolic interplay in human cells in the context of disease. We have applied nuclear magnetic resonance (NMR) spectroscopy approaches to e作者: emulsify 時(shí)間: 2025-3-23 22:46
13C Isotope Labeling and Mass Spectrometric Isotope Enrichment Analysis in Acute Brain Slices,arious experimental conditions. This information is particularly relevant for the study and functional understanding of brain metabolic heterogeneity. We here describe a protocol for the analysis of metabolic enrichment analysis upon feeding of murine acute cerebellar slices with .C-labeled substrat作者: 表示問 時(shí)間: 2025-3-24 05:07
Metabolite Analyses Using Nuclear Magnetic Resonance (NMR) Spectroscopy in Plasma of Patients with ht metabolites and lipids. NMR spectroscopy is a powerful approach when applied to the high-throughput analysis of plasma or serum samples allowing, in addition, the detection of total proteins, lipoproteins, and signals arising from the glycosylation of circulating acute-phase proteins. Here, we de作者: 使混合 時(shí)間: 2025-3-24 10:27
Screening Kinase-Dependent Phosphorylation of Key Metabolic Reprogramming Regulators,thesis of new cell constituents needed for cell growth and division. The M2 isoform of pyruvate kinase (PKM2) catalyzes the last reaction of the glycolytic process. PKM2 promotes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, generating ATP and releasing pyruvate. This rate作者: 多余 時(shí)間: 2025-3-24 14:33
Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPsis. GAPDH catalyzes the nicotinamide adenine dinucleotide (NAD.)- and inorganic phosphate-dependent oxidation and phosphorylation of glyceraldehyde phosphate (GAP) to form 1,3-bisphosphoglycerate (BPG). As part of its mechanism of action, GAPDH employs a redox-sensitive cysteine that serves as a nu作者: 指令 時(shí)間: 2025-3-24 18:24 作者: SNEER 時(shí)間: 2025-3-24 20:12 作者: Flat-Feet 時(shí)間: 2025-3-25 00:25 作者: Orchiectomy 時(shí)間: 2025-3-25 05:37
Methods for Monitoring Mitochondrial Biogenesis and Turnover in Cultured Hepatocytes and Mouse Liveoscopy analysis revealed that majority of mitochondria were spatially heterogeneous with proteins from simultaneous new synthesis, maturation, and turnover in hepatocytes. MitoTimer reporter assay can specifically target to mitochondria and be used to monitor mitochondrial biogenesis and maturation 作者: obeisance 時(shí)間: 2025-3-25 07:28 作者: 運(yùn)氣 時(shí)間: 2025-3-25 14:23
Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPce decreased GAPDH activity results in the rerouting of carbon flux away from glycolysis and toward the pentose phosphate pathway to produce the key cellular reductant and antioxidant, NADPH. Thus, the ability to probe GAPDH oxidation and activity provides an important snapshot of the intracellular 作者: 擋泥板 時(shí)間: 2025-3-25 17:37 作者: collagen 時(shí)間: 2025-3-25 20:36 作者: erythema 時(shí)間: 2025-3-26 03:17 作者: cringe 時(shí)間: 2025-3-26 07:02 作者: heterogeneous 時(shí)間: 2025-3-26 09:03 作者: carotid-bruit 時(shí)間: 2025-3-26 13:10
Marta Iborra Pernichi,Jonathan Ruiz García,Nuria Martínez-Martín作者: 增長(zhǎng) 時(shí)間: 2025-3-26 17:07
María Eugenia Monge,Manuela R. Martinefski,Mariela Bollini,Lucas B. Pontel作者: 溫室 時(shí)間: 2025-3-27 00:51 作者: BRIBE 時(shí)間: 2025-3-27 03:00
Raquel Saborano,Emma Shepherd,Ulrich L. Günther,Patricia F. Lalor作者: excursion 時(shí)間: 2025-3-27 08:56 作者: CANE 時(shí)間: 2025-3-27 10:47 作者: 細(xì)胞 時(shí)間: 2025-3-27 16:39 作者: 流浪 時(shí)間: 2025-3-27 20:58 作者: 幻想 時(shí)間: 2025-3-28 00:23
1064-3745 ation advice from the experts.This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-product作者: Tailor 時(shí)間: 2025-3-28 02:53 作者: 我們的面粉 時(shí)間: 2025-3-28 07:32 作者: Recessive 時(shí)間: 2025-3-28 13:51
Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells,developed over the last few years that enable such non-disruptive monitoring with high spatiotemporal resolution. In this chapter, we describe the use of these genetically encoded sensors to directly monitor H.O. dynamics in yeast and cultured mammalian cells.作者: 沉積物 時(shí)間: 2025-3-28 15:54 作者: 刀鋒 時(shí)間: 2025-3-28 19:26 作者: 無法破譯 時(shí)間: 2025-3-29 01:43
In Vivo Tissue Lipid Uptake in Antisense Oligonucleotide (ASO)-Treated Mice,al gavage. To ascertain more precisely the capacity of each tissue for fatty acid uptake, not considering the intestinal barrier, the intravenous (IV) administration of radiolabeled lipids is also described.作者: Inkling 時(shí)間: 2025-3-29 05:02 作者: Serenity 時(shí)間: 2025-3-29 10:10 作者: 頌揚(yáng)國(guó)家 時(shí)間: 2025-3-29 14:14
Quantitation of Glutamine Synthetase 1 Activity in ,formation with FeCl.. Fixed-time assays and those based on monitoring the time-course of product formation at different reaction times are described. The protocols can be adapted to quantify glutamine synthetase activity on biological materials other than ..作者: 細(xì)查 時(shí)間: 2025-3-29 17:48 作者: 女上癮 時(shí)間: 2025-3-29 23:09
Isolation of Mitochondria from Mouse Tissues for Functional Analysis,ation from different tissues to optimize mitochondrial yield and function. This protocol offers a unified resource for preparations of isolated mitochondria from mouse liver, kidney, heart, brain, skeletal muscle, and brown and white adipose tissue suitable for functional analysis.作者: gonioscopy 時(shí)間: 2025-3-30 00:54
Quantitation of Glutathione and Oxidized Glutathione Ratios from Biological Matrices Using LC-MS/MSs an ideal method to measure the GSH/GSSG ratio and can be applied to a variety of biological matrices and disease models. The following chapter details the design, optimization, and execution of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay to measure the GSH/GSSG ratio.作者: 我還要背著他 時(shí)間: 2025-3-30 05:48
Metabolite Analyses Using Nuclear Magnetic Resonance (NMR) Spectroscopy in Plasma of Patients with n addition, the detection of total proteins, lipoproteins, and signals arising from the glycosylation of circulating acute-phase proteins. Here, we describe the usage of NMR spectroscopy for profiling the plasma or serum of patients with prostate cancer.作者: DRILL 時(shí)間: 2025-3-30 09:29
Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation,Hypoxia and its signalling pathway play a key role in human physiology and a variety of diseases. Alterations in histone methylation coordinate transcriptional responses to hypoxia. Here, we detail a fixed cell immunofluorescence method for quantifying hypoxia-induced changes in histone methylation, exemplified by the measurement of H3K27me3.作者: Cryptic 時(shí)間: 2025-3-30 15:53
Salvatore Papa,Concetta BubiciIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: MAL 時(shí)間: 2025-3-30 17:37 作者: Harrowing 時(shí)間: 2025-3-30 20:56 作者: mastoid-bone 時(shí)間: 2025-3-31 01:20 作者: Engaged 時(shí)間: 2025-3-31 05:31 作者: Acquired 時(shí)間: 2025-3-31 10:29 作者: aggressor 時(shí)間: 2025-3-31 16:04
13C Isotope Labeling and Mass Spectrometric Isotope Enrichment Analysis in Acute Brain Slices,arious experimental conditions. This information is particularly relevant for the study and functional understanding of brain metabolic heterogeneity. We here describe a protocol for the analysis of metabolic enrichment analysis upon feeding of murine acute cerebellar slices with .C-labeled substrates.作者: 結(jié)構(gòu) 時(shí)間: 2025-3-31 21:31
Rainer T?lle,Klaus WindgassenrbeiterInnen in der Gemeindepastoral umsetzen l??t (vgl. dazu Behrenberg, Fa?nacht, 1994a, S. 195–199). Die nunmehr sechsj?hrigen Erfahrungen mit unserem ?.“ und den derzeitigen Stand des Konzeptes m?chten wir im folgenden in zwei Schritten vorstellen. Zun?chst werden wir etwas zur ?Geschichte” unse作者: Ankylo- 時(shí)間: 2025-4-1 00:21 作者: Defense 時(shí)間: 2025-4-1 05:53
Wilbert S. Aronow,William H. Frishmanhood in the shadow of churches, families, and a small southern town in the 1990’s. Alongside academic and media portrayals that generally only acknowledge binary sexual and gender options, Cigarettes & Wine off978-94-6300-929-4作者: 不真 時(shí)間: 2025-4-1 08:08 作者: 左右連貫 時(shí)間: 2025-4-1 13:18 作者: 反抗者 時(shí)間: 2025-4-1 16:11
