標(biāo)題: Titlebook: Metabolic Flux Analysis in Eukaryotic Cells; Methods and Protocol Deepak Nagrath Book 2020 Springer Science+Business Media, LLC, part of Sp [打印本頁] 作者: gloomy 時(shí)間: 2025-3-21 16:37
書目名稱Metabolic Flux Analysis in Eukaryotic Cells影響因子(影響力)
書目名稱Metabolic Flux Analysis in Eukaryotic Cells影響因子(影響力)學(xué)科排名
書目名稱Metabolic Flux Analysis in Eukaryotic Cells網(wǎng)絡(luò)公開度
書目名稱Metabolic Flux Analysis in Eukaryotic Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Metabolic Flux Analysis in Eukaryotic Cells被引頻次
書目名稱Metabolic Flux Analysis in Eukaryotic Cells被引頻次學(xué)科排名
書目名稱Metabolic Flux Analysis in Eukaryotic Cells年度引用
書目名稱Metabolic Flux Analysis in Eukaryotic Cells年度引用學(xué)科排名
書目名稱Metabolic Flux Analysis in Eukaryotic Cells讀者反饋
書目名稱Metabolic Flux Analysis in Eukaryotic Cells讀者反饋學(xué)科排名
作者: 組成 時(shí)間: 2025-3-21 22:18 作者: 引起痛苦 時(shí)間: 2025-3-22 01:29 作者: Diastole 時(shí)間: 2025-3-22 05:51
Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13Cmetabolites significantly add to the number of analytes. In this protocol chapter, we discuss two liquid chromatography methods, namely, reverse phase ion-pairing and hydrophilic interaction chromatography (HILIC) that together can separate a wide variety of metabolites that are typically used for .C metabolic flux analysis.作者: 發(fā)酵 時(shí)間: 2025-3-22 11:33 作者: CRUC 時(shí)間: 2025-3-22 14:47
Inferring Metabolic Flux from Time-Course Metabolomics,hm, a genome-scale modeling approach that uses time-course metabolomics to predict dynamic flux rewiring during transitions between metabolic states. This chapter provides a protocol for applying DFA to characterize the dynamic metabolic activity of various cancer cell lines.作者: 竊喜 時(shí)間: 2025-3-22 19:09 作者: 公社 時(shí)間: 2025-3-23 00:19
Quantifying Nitric Oxide Flux Distributions,ed protocol, which includes experimental measurements and computational modeling, to estimate the NO flux distribution in an . culture. Those fluxes will have uncertainty associated with them and we also discuss how further experiments and modeling can be employed for flux refinement.作者: CANON 時(shí)間: 2025-3-23 01:46 作者: 凹處 時(shí)間: 2025-3-23 08:07 作者: 被告 時(shí)間: 2025-3-23 13:40
Metabolic Network Reconstructions to Predict Drug Targets and Off-Target Effects,ent off-target effects. Here, we summarize available methods to identify drug targets and off-target effects using either reaction-centric, gene-centric, or metabolite-centric approaches with genome-scale metabolic network reconstructions.作者: 天然熱噴泉 時(shí)間: 2025-3-23 16:28
Thermodynamic Approaches in Flux Analysis,x balance analysis. Some of these methods have focused on adding thermodynamics constraints to eliminate non-physical fluxes or inconsistencies in the metabolic system. Here, we review the main different approaches and how they tackle the different class of problems.作者: insincerity 時(shí)間: 2025-3-23 19:54
1064-3745 Eukaryotic Cells: Methods and Protocols.?is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases..978-1-0716-0161-7978-1-0716-0159-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: MARS 時(shí)間: 2025-3-23 22:25 作者: 吼叫 時(shí)間: 2025-3-24 06:00
lly, the comoving density ..(.). The bias therefore has implications for the histories of gas consumption, star formation, and metal production in the damped Ly. systems. In particular, it solves a puzzle that has arisen in attempts to understand the chemical evolution in the damped Ly. systems (the作者: Enzyme 時(shí)間: 2025-3-24 09:53
Teresa Mairinger,Stephen Hannlly, the comoving density ..(.). The bias therefore has implications for the histories of gas consumption, star formation, and metal production in the damped Ly. systems. In particular, it solves a puzzle that has arisen in attempts to understand the chemical evolution in the damped Ly. systems (the作者: GNAW 時(shí)間: 2025-3-24 11:35 作者: gentle 時(shí)間: 2025-3-24 17:06 作者: 2否定 時(shí)間: 2025-3-24 20:49 作者: LITHE 時(shí)間: 2025-3-25 00:52
Patricia Altea-Manzano,Dorien Broekaert,Jo?o A. G. Duarte,Juan Fernández-García,Mélanie Planque,Saracally large energy barriers . which force the system to get trapped, depending on its history (initial configuration), in one of its degenerate (local) minima. The system thus becomes “nonergodic” and a spin glass may be described by a nontrivial order parameter distribution [.] in the thermodynamic作者: 享樂主義者 時(shí)間: 2025-3-25 04:26
Diana Széliová,Harald Schoeny,?pela Knez,Christina Troyer,Cristina Coman,Evelyn Rampler,Gunda Koelleactions in set-ups of realistic experiments which allow for preparation and detection, before we can decide whether or not these systems describe reality. As this question is of central importance I insert here a first chapter on the description of scattering processes in what is called ., before tu作者: Genistein 時(shí)間: 2025-3-25 08:30
Darshan M. Sivaloganathan,Xuanqing Wan,Mark P. Brynildsen of translation in time and space. The origin of this somewhat ascetic formula (9.1) was explained in Sect. 6.3.4. For this reason a short summary may be sufficient at this point: The first factor expi. · (. . represents the action of the translation.作者: Cougar 時(shí)間: 2025-3-25 14:08 作者: Sinus-Rhythm 時(shí)間: 2025-3-25 16:14
Abhinav Achreja,Noah Meurs,Deepak Nagrath of translation in time and space. The origin of this somewhat ascetic formula (9.1) was explained in Sect. 6.3.4. For this reason a short summary may be sufficient at this point: The first factor expi. · (. . represents the action of the translation.作者: Oration 時(shí)間: 2025-3-25 20:36 作者: fertilizer 時(shí)間: 2025-3-26 01:04
Vitaly A. Selivanov,Silvia Marin,Josep Tarragó-Celada,Andrew N. Lane,Richard M. Higashi,Teresa W.-M.ies in lakes and rivers, through the drinking water supply, or through water used in renal dialysis. Examples of human illness from these three sources occur. Water treatment can be developed to extract or destroy the toxins. Human epidemiological studies are needed to support the experimental anima作者: MELD 時(shí)間: 2025-3-26 07:19
Scott Campit,Sriram Chandrasekaranrom a model consisting of two-level systems, and describe the oscillation and amplification processes in the context of quantum mechanical interaction between the electromagnetic wave and the atomic system in a cavity. The photon number in an oscillating mode increases with the degree of population 作者: Macronutrients 時(shí)間: 2025-3-26 12:04
Kristopher Rawls,Bonnie V. Dougherty,Jason Papinare discussed in this chapter can be classified according to how many times this interaction works in each nonlinear optical response. The lowest-order nonlinear optical responses are sum-frequency and second-harmonic generation (SHG). These will be discussed in Sect. 6.1. This sum-frequency and hig作者: Figate 時(shí)間: 2025-3-26 16:05 作者: Cleave 時(shí)間: 2025-3-26 19:19 作者: FLACK 時(shí)間: 2025-3-27 01:00
g. Not only do these phenomena rely on the concepts presented in this book, but magnetic propertiesare often an important clue to our understanding of new materials (e.g., high-temperature superconductors). Their magnetic properties, studied by susceptibility measurements, nuclear magnetic resonance作者: 中世紀(jì) 時(shí)間: 2025-3-27 02:43
Book 2020, and tips on troubleshooting and avoiding known pitfalls..?.Cutting-edge and comprehensive,?.Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols.?is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases..作者: 不如屎殼郎 時(shí)間: 2025-3-27 07:25
Determination of Isotopologue and Tandem Mass Isotopologue Ratios Using Gas Chromatography Chemicalization steps and the resulting need for interference correction for natural abundant isotopes..Here, we describe a workflow based on GC-high resolution mass spectrometry with chemical ionization for the analysis of carbon-isotopologue distributions and some positional labeling information of primar作者: MOCK 時(shí)間: 2025-3-27 10:34 作者: 過份艷麗 時(shí)間: 2025-3-27 14:08 作者: foreign 時(shí)間: 2025-3-27 21:16
Quantifying Metabolic Transfer Mediated by Extracellular Vesicles Using Exo-MFA: An Integrated Empion a recipient cell type. This is accomplished by creating EV-depleted culture medium, producing isotope-labeled EVs from the source cells, isolating the labeled EVs from the culture supernatant, culturing the recipient cells in the presence of the labeled EVs, and measuring the resulting metabolite作者: Offstage 時(shí)間: 2025-3-27 23:55
Single-cell Digital Twins for Cancer Preclinical Investigation,tumour heterogeneous dependency on nutrients consumption and metabolism by multiple co-existing subclones. Currently, fluxomics approaches just match with the necessity of characterizing the overall flux distribution of cells within given samples, by disregarding possible heterogeneous behaviors. Fo作者: 令人苦惱 時(shí)間: 2025-3-28 02:21 作者: 該得 時(shí)間: 2025-3-28 10:10
Damini Jaiswal,Anjali Mittal,Deepak Nagrath,Pramod P. Wangikartems: in other words, it is a phenomenon persistent under small Hamiltonian perturbations. Moreover, generally, for any such system the union of quasi-periodic 978-3-540-62025-9978-3-540-49613-7Series ISSN 0075-8434 Series E-ISSN 1617-9692 作者: 預(yù)兆好 時(shí)間: 2025-3-28 13:09 作者: 分貝 時(shí)間: 2025-3-28 16:57 作者: 省略 時(shí)間: 2025-3-28 19:36 作者: 集中營(yíng) 時(shí)間: 2025-3-29 00:01 作者: 神秘 時(shí)間: 2025-3-29 07:04
Deepak NagrathIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: intoxicate 時(shí)間: 2025-3-29 09:54 作者: exceptional 時(shí)間: 2025-3-29 14:51
Determination of Isotopologue and Tandem Mass Isotopologue Ratios Using Gas Chromatography Chemicalof assessing absolute intracellular metabolic fluxes. Resulting from feeding the organism of interest with a specifically isotope-labeled substrate, the principal characteristics of these labeling experiments are the metabolites’ non-naturally distributed isotopologue patterns. For the purpose of in作者: 個(gè)阿姨勾引你 時(shí)間: 2025-3-29 19:35 作者: BRINK 時(shí)間: 2025-3-29 23:47
Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C). Liquid chromatography coupled with mass spectrometry (LC/MS) has emerged as a frontrunner technique that combines two orthogonal separation strategies. While metabolomics requires separation of monoisotopic peaks, .C-MFA imposes additional demands for chromatographic separation as isotopologs of 作者: Defense 時(shí)間: 2025-3-30 02:23
Deuterium Tracing to Interrogate Compartment-Specific NAD(P)H Metabolism in Cultured Mammalian Cellfer between molecules and organelles is commonly facilitated by pyridine nucleotides such as nicotinamide adenine dinucleotide phosphate (NADPH) and nicotinamide adenine dinucleotide (NADH). While often taken for granted, these metabolic reactions are critically important for maintaining redox homeo作者: bile648 時(shí)間: 2025-3-30 05:28 作者: Coronation 時(shí)間: 2025-3-30 09:02 作者: 教育學(xué) 時(shí)間: 2025-3-30 14:11
Robust Analytical Methods for the Accurate Quantification of the Total Biomass Composition of Mammas often rely on generic data for biomass composition, e.g. collected from similar organisms. This leads to inaccurate predictions, because biomass composition varies between different cell lines, conditions, and growth phases. In this chapter we present protocols for the determination of the biomass作者: anticipate 時(shí)間: 2025-3-30 18:36 作者: 階層 時(shí)間: 2025-3-30 21:26 作者: 大包裹 時(shí)間: 2025-3-31 04:02
Quantifying Metabolic Transfer Mediated by Extracellular Vesicles Using Exo-MFA: An Integrated Empi DNA, RNA, miRNA, and, most recently, metabolites. These particles can travel through the intercellular space and bloodstream to have regulatory effects on distant recipients. When an EV reaches a target cell, it is taken up and degraded to release its contents for utilization within the cell. In ad作者: Exuberance 時(shí)間: 2025-3-31 05:04 作者: 微塵 時(shí)間: 2025-3-31 10:48 作者: 開頭 時(shí)間: 2025-3-31 15:37
Inferring Metabolic Flux from Time-Course Metabolomics,ell differentiation and proliferation. While experimental tools like time-course metabolomics and flux tracing can measure the dynamics of a few pathways, they are unable to infer fluxes at the whole network level. To address this limitation, we have developed the Dynamic Flux Activity (DFA) algorit作者: HATCH 時(shí)間: 2025-3-31 19:48
Metabolic Network Reconstructions to Predict Drug Targets and Off-Target Effects, methods, such as the use of metabolic network reconstructions, may provide a cost-effective platform to test new hypotheses for drug targets and prevent off-target effects. Here, we summarize available methods to identify drug targets and off-target effects using either reaction-centric, gene-centr作者: Mettle 時(shí)間: 2025-4-1 00:56
